Biochemical properties of the 75-kDa tumor necrosis factor receptor. Characterization of ligand binding, internalization, and receptor phosphorylation

An expression plasmid encoding the human 75-kDa tumor necrosis factor (TNF) type 2 receptor (TNF-R2) was constructed and used to generate a stable human cell line (293/TNF-R2) overexpressing TNF-R2. Ligand binding analysis revealed high affinity binding (Kd = 0.2 nM) with approximately 94,000 +/- 7,...

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Veröffentlicht in:	The Journal of biological chemistry 1992-10, Vol.267 (29), p.21172-21178
Hauptverfasser:	PENNICA, D, LAM, V. T, MIZE, N. K, WEBER, R. F, LEWIS, M, FENDLY, B. M, LIPARI, M. T, GOEDDEL, D. V
Format:	Artikel
Sprache:	eng
Schlagworte:	biochemical characteristics Biological and medical sciences Cell receptors Cell structures and functions Chromatography, Affinity Fundamental and applied biological sciences. Psychology Gene Library Humans Kinetics Lymphotoxin-alpha - pharmacology man Miscellaneous Molecular and cellular biology Molecular Weight Phosphorylation Plasmids receptors Receptors, Cell Surface - genetics Receptors, Cell Surface - isolation & purification Receptors, Cell Surface - metabolism Receptors, Tumor Necrosis Factor Recombinant Proteins - metabolism RNA, Messenger - genetics Tetradecanoylphorbol Acetate - pharmacology Transfection Tumor Cells, Cultured tumor necrosis factor Tumor Necrosis Factor-alpha - metabolism Tumor Necrosis Factor-alpha - pharmacology
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