Circulating tumor cell enumeration with a combination of epithelial cell adhesion molecule- and cell-surface vimentin-based methods for monitoring breast cancer therapeutic response
Detection, isolation, and enumeration of circulating tumor cells (CTCs) from cancer patients has become an important modality in clinical management of patients with breast cancer. Although CellSearch, an epithelial cell adhesion molecule (EpCAM)-based method that is used to isolate epithelial CTCs,...
Gespeichert in:
| Veröffentlicht in: | Clinical chemistry (Baltimore, Md.) Md.), 2015-01, Vol.61 (1), p.259-266 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 266 |
|---|---|
| container_issue | 1 |
| container_start_page | 259 |
| container_title | Clinical chemistry (Baltimore, Md.) |
| container_volume | 61 |
| creator | Satelli, Arun Brownlee, Zachary Mitra, Abhisek Meng, Qing H Li, Shulin |
| description | Detection, isolation, and enumeration of circulating tumor cells (CTCs) from cancer patients has become an important modality in clinical management of patients with breast cancer. Although CellSearch, an epithelial cell adhesion molecule (EpCAM)-based method that is used to isolate epithelial CTCs, has gained prominence, its inability to detect mesenchymal CTCs from breast cancer patients raises concerns regarding its utility in clinical management.
To address this gap in technology, we recently discovered the utility of cell-surface vimentin (CSV) as a marker for detecting mesenchymal CTCs from sarcoma tumors. In the present study, we tested the sensitivity and specificity of detecting CTCs from blood collected at a random time during therapy from each of 58 patients with metastatic breast cancer by use of 84-1 (a monoclonal antibody against CSV to detect epithelial/mesenchymal-transition CTCs) and CellSearch methods. Additionally, we tested the possibility of improving the sensitivity and specificity of detection by use of additional parameters including nuclear EpCAM localization and epithelial mesenchymal ratios.
CTC counts with CSV were significant (P = 0.0053) in differentiating populations responsive and nonresponsive to treatment compared with CTC counts with CellSearch (P = 0.0564). The specificity of CTC detection was found to be highest when the sum of CTC counts from the 2 methods was above a threshold of 8 CTCs/7.5 mL.
The sum of CTC counts from the CellSearch and CSV methods appears to provide new insights for assessment of therapeutic response and thus provides a new approach to personalized medicine in breast cancer patients. |
| doi_str_mv | 10.1373/clinchem.2014.228122 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1641427197</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1641427197</sourcerecordid><originalsourceid>FETCH-LOGICAL-c381t-352298fe1a80449620147570d13d42b7cad03e413fe8311ab570e59d5c17db693</originalsourceid><addsrcrecordid>eNpdUctu1TAUtBCIXlr-ACFLbNjk1sd24mSJrspDqsSGriPHPuG6iu1gJyA-rP-HQ1oWrCyfmTkz9hDyBtgRhBLXZnLBnNEfOQN55LwFzp-RA9SCVW3dwHNyYIx1VQdSXZBXOd-Xq1Rt85Jc8FqIRoE6kIeTS2ad9OLCd7qsPiZqcJoohtVjKuMY6C-3nKmmJvrBhX0UR4pzGePk9LQrtD1j3jAfJywrsaI62L9Yldc0aoP0p_MYilU16IyWelzO0WY6Flcfg1ti2mIMCXVeqNHBYKLFJOkZ18UZmjDPMWS8Ii9GPWV8_XhekruPN99On6vbr5--nD7cVka0sFSi5rxrRwTdMim7ZvspVStmQVjJB2W0ZQIliBFbAaCHgmHd2dqAskPTiUvyft87p_hjxbz03uXtRTpgXHMPjQTJFXSqUN_9R72PawolXWE1UKuublhhyZ1lUsw54djPyXmdfvfA-q3W_qnWfgvb77UW2dvH5evg0f4TPfUo_gB9W6PZ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1661579560</pqid></control><display><type>article</type><title>Circulating tumor cell enumeration with a combination of epithelial cell adhesion molecule- and cell-surface vimentin-based methods for monitoring breast cancer therapeutic response</title><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Satelli, Arun ; Brownlee, Zachary ; Mitra, Abhisek ; Meng, Qing H ; Li, Shulin</creator><creatorcontrib>Satelli, Arun ; Brownlee, Zachary ; Mitra, Abhisek ; Meng, Qing H ; Li, Shulin</creatorcontrib><description>Detection, isolation, and enumeration of circulating tumor cells (CTCs) from cancer patients has become an important modality in clinical management of patients with breast cancer. Although CellSearch, an epithelial cell adhesion molecule (EpCAM)-based method that is used to isolate epithelial CTCs, has gained prominence, its inability to detect mesenchymal CTCs from breast cancer patients raises concerns regarding its utility in clinical management.
To address this gap in technology, we recently discovered the utility of cell-surface vimentin (CSV) as a marker for detecting mesenchymal CTCs from sarcoma tumors. In the present study, we tested the sensitivity and specificity of detecting CTCs from blood collected at a random time during therapy from each of 58 patients with metastatic breast cancer by use of 84-1 (a monoclonal antibody against CSV to detect epithelial/mesenchymal-transition CTCs) and CellSearch methods. Additionally, we tested the possibility of improving the sensitivity and specificity of detection by use of additional parameters including nuclear EpCAM localization and epithelial mesenchymal ratios.
CTC counts with CSV were significant (P = 0.0053) in differentiating populations responsive and nonresponsive to treatment compared with CTC counts with CellSearch (P = 0.0564). The specificity of CTC detection was found to be highest when the sum of CTC counts from the 2 methods was above a threshold of 8 CTCs/7.5 mL.
The sum of CTC counts from the CellSearch and CSV methods appears to provide new insights for assessment of therapeutic response and thus provides a new approach to personalized medicine in breast cancer patients.</description><identifier>ISSN: 0009-9147</identifier><identifier>EISSN: 1530-8561</identifier><identifier>DOI: 10.1373/clinchem.2014.228122</identifier><identifier>PMID: 25336717</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Adhesion ; Antibodies, Monoclonal - immunology ; Antigens, Neoplasm - blood ; Biomarkers, Tumor - blood ; Breast cancer ; Breast Neoplasms - blood ; Breast Neoplasms - drug therapy ; Breast Neoplasms - metabolism ; Breast Neoplasms - pathology ; Cell Adhesion Molecules - blood ; Cell Count ; Cell Membrane - metabolism ; Epithelial Cell Adhesion Molecule ; Epithelial-Mesenchymal Transition - drug effects ; Female ; Humans ; Medical research ; Metastasis ; Methods ; Monitoring methods ; Neoplasm Metastasis ; Neoplastic Cells, Circulating - drug effects ; Neoplastic Cells, Circulating - pathology ; Patients ; Pilot Projects ; Retrospective Studies ; Sarcoma ; Studies ; Treatment Outcome ; Vimentin - immunology ; Vimentin - metabolism</subject><ispartof>Clinical chemistry (Baltimore, Md.), 2015-01, Vol.61 (1), p.259-266</ispartof><rights>2014 American Association for Clinical Chemistry.</rights><rights>Copyright American Association for Clinical Chemistry Jan 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-352298fe1a80449620147570d13d42b7cad03e413fe8311ab570e59d5c17db693</citedby><cites>FETCH-LOGICAL-c381t-352298fe1a80449620147570d13d42b7cad03e413fe8311ab570e59d5c17db693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25336717$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Satelli, Arun</creatorcontrib><creatorcontrib>Brownlee, Zachary</creatorcontrib><creatorcontrib>Mitra, Abhisek</creatorcontrib><creatorcontrib>Meng, Qing H</creatorcontrib><creatorcontrib>Li, Shulin</creatorcontrib><title>Circulating tumor cell enumeration with a combination of epithelial cell adhesion molecule- and cell-surface vimentin-based methods for monitoring breast cancer therapeutic response</title><title>Clinical chemistry (Baltimore, Md.)</title><addtitle>Clin Chem</addtitle><description>Detection, isolation, and enumeration of circulating tumor cells (CTCs) from cancer patients has become an important modality in clinical management of patients with breast cancer. Although CellSearch, an epithelial cell adhesion molecule (EpCAM)-based method that is used to isolate epithelial CTCs, has gained prominence, its inability to detect mesenchymal CTCs from breast cancer patients raises concerns regarding its utility in clinical management.
To address this gap in technology, we recently discovered the utility of cell-surface vimentin (CSV) as a marker for detecting mesenchymal CTCs from sarcoma tumors. In the present study, we tested the sensitivity and specificity of detecting CTCs from blood collected at a random time during therapy from each of 58 patients with metastatic breast cancer by use of 84-1 (a monoclonal antibody against CSV to detect epithelial/mesenchymal-transition CTCs) and CellSearch methods. Additionally, we tested the possibility of improving the sensitivity and specificity of detection by use of additional parameters including nuclear EpCAM localization and epithelial mesenchymal ratios.
CTC counts with CSV were significant (P = 0.0053) in differentiating populations responsive and nonresponsive to treatment compared with CTC counts with CellSearch (P = 0.0564). The specificity of CTC detection was found to be highest when the sum of CTC counts from the 2 methods was above a threshold of 8 CTCs/7.5 mL.
The sum of CTC counts from the CellSearch and CSV methods appears to provide new insights for assessment of therapeutic response and thus provides a new approach to personalized medicine in breast cancer patients.</description><subject>Adhesion</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigens, Neoplasm - blood</subject><subject>Biomarkers, Tumor - blood</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - blood</subject><subject>Breast Neoplasms - drug therapy</subject><subject>Breast Neoplasms - metabolism</subject><subject>Breast Neoplasms - pathology</subject><subject>Cell Adhesion Molecules - blood</subject><subject>Cell Count</subject><subject>Cell Membrane - metabolism</subject><subject>Epithelial Cell Adhesion Molecule</subject><subject>Epithelial-Mesenchymal Transition - drug effects</subject><subject>Female</subject><subject>Humans</subject><subject>Medical research</subject><subject>Metastasis</subject><subject>Methods</subject><subject>Monitoring methods</subject><subject>Neoplasm Metastasis</subject><subject>Neoplastic Cells, Circulating - drug effects</subject><subject>Neoplastic Cells, Circulating - pathology</subject><subject>Patients</subject><subject>Pilot Projects</subject><subject>Retrospective Studies</subject><subject>Sarcoma</subject><subject>Studies</subject><subject>Treatment Outcome</subject><subject>Vimentin - immunology</subject><subject>Vimentin - metabolism</subject><issn>0009-9147</issn><issn>1530-8561</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdUctu1TAUtBCIXlr-ACFLbNjk1sd24mSJrspDqsSGriPHPuG6iu1gJyA-rP-HQ1oWrCyfmTkz9hDyBtgRhBLXZnLBnNEfOQN55LwFzp-RA9SCVW3dwHNyYIx1VQdSXZBXOd-Xq1Rt85Jc8FqIRoE6kIeTS2ad9OLCd7qsPiZqcJoohtVjKuMY6C-3nKmmJvrBhX0UR4pzGePk9LQrtD1j3jAfJywrsaI62L9Yldc0aoP0p_MYilU16IyWelzO0WY6Flcfg1ti2mIMCXVeqNHBYKLFJOkZ18UZmjDPMWS8Ii9GPWV8_XhekruPN99On6vbr5--nD7cVka0sFSi5rxrRwTdMim7ZvspVStmQVjJB2W0ZQIliBFbAaCHgmHd2dqAskPTiUvyft87p_hjxbz03uXtRTpgXHMPjQTJFXSqUN_9R72PawolXWE1UKuublhhyZ1lUsw54djPyXmdfvfA-q3W_qnWfgvb77UW2dvH5evg0f4TPfUo_gB9W6PZ</recordid><startdate>201501</startdate><enddate>201501</enddate><creator>Satelli, Arun</creator><creator>Brownlee, Zachary</creator><creator>Mitra, Abhisek</creator><creator>Meng, Qing H</creator><creator>Li, Shulin</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4U-</scope><scope>7QO</scope><scope>7RV</scope><scope>7TM</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>S0X</scope><scope>7X8</scope></search><sort><creationdate>201501</creationdate><title>Circulating tumor cell enumeration with a combination of epithelial cell adhesion molecule- and cell-surface vimentin-based methods for monitoring breast cancer therapeutic response</title><author>Satelli, Arun ; Brownlee, Zachary ; Mitra, Abhisek ; Meng, Qing H ; Li, Shulin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-352298fe1a80449620147570d13d42b7cad03e413fe8311ab570e59d5c17db693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adhesion</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigens, Neoplasm - blood</topic><topic>Biomarkers, Tumor - blood</topic><topic>Breast cancer</topic><topic>Breast Neoplasms - blood</topic><topic>Breast Neoplasms - drug therapy</topic><topic>Breast Neoplasms - metabolism</topic><topic>Breast Neoplasms - pathology</topic><topic>Cell Adhesion Molecules - blood</topic><topic>Cell Count</topic><topic>Cell Membrane - metabolism</topic><topic>Epithelial Cell Adhesion Molecule</topic><topic>Epithelial-Mesenchymal Transition - drug effects</topic><topic>Female</topic><topic>Humans</topic><topic>Medical research</topic><topic>Metastasis</topic><topic>Methods</topic><topic>Monitoring methods</topic><topic>Neoplasm Metastasis</topic><topic>Neoplastic Cells, Circulating - drug effects</topic><topic>Neoplastic Cells, Circulating - pathology</topic><topic>Patients</topic><topic>Pilot Projects</topic><topic>Retrospective Studies</topic><topic>Sarcoma</topic><topic>Studies</topic><topic>Treatment Outcome</topic><topic>Vimentin - immunology</topic><topic>Vimentin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Satelli, Arun</creatorcontrib><creatorcontrib>Brownlee, Zachary</creatorcontrib><creatorcontrib>Mitra, Abhisek</creatorcontrib><creatorcontrib>Meng, Qing H</creatorcontrib><creatorcontrib>Li, Shulin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>University Readers</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Science Journals</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Satelli, Arun</au><au>Brownlee, Zachary</au><au>Mitra, Abhisek</au><au>Meng, Qing H</au><au>Li, Shulin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Circulating tumor cell enumeration with a combination of epithelial cell adhesion molecule- and cell-surface vimentin-based methods for monitoring breast cancer therapeutic response</atitle><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle><addtitle>Clin Chem</addtitle><date>2015-01</date><risdate>2015</risdate><volume>61</volume><issue>1</issue><spage>259</spage><epage>266</epage><pages>259-266</pages><issn>0009-9147</issn><eissn>1530-8561</eissn><abstract>Detection, isolation, and enumeration of circulating tumor cells (CTCs) from cancer patients has become an important modality in clinical management of patients with breast cancer. Although CellSearch, an epithelial cell adhesion molecule (EpCAM)-based method that is used to isolate epithelial CTCs, has gained prominence, its inability to detect mesenchymal CTCs from breast cancer patients raises concerns regarding its utility in clinical management.
To address this gap in technology, we recently discovered the utility of cell-surface vimentin (CSV) as a marker for detecting mesenchymal CTCs from sarcoma tumors. In the present study, we tested the sensitivity and specificity of detecting CTCs from blood collected at a random time during therapy from each of 58 patients with metastatic breast cancer by use of 84-1 (a monoclonal antibody against CSV to detect epithelial/mesenchymal-transition CTCs) and CellSearch methods. Additionally, we tested the possibility of improving the sensitivity and specificity of detection by use of additional parameters including nuclear EpCAM localization and epithelial mesenchymal ratios.
CTC counts with CSV were significant (P = 0.0053) in differentiating populations responsive and nonresponsive to treatment compared with CTC counts with CellSearch (P = 0.0564). The specificity of CTC detection was found to be highest when the sum of CTC counts from the 2 methods was above a threshold of 8 CTCs/7.5 mL.
The sum of CTC counts from the CellSearch and CSV methods appears to provide new insights for assessment of therapeutic response and thus provides a new approach to personalized medicine in breast cancer patients.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>25336717</pmid><doi>10.1373/clinchem.2014.228122</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0009-9147 |
| ispartof | Clinical chemistry (Baltimore, Md.), 2015-01, Vol.61 (1), p.259-266 |
| issn | 0009-9147 1530-8561 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1641427197 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Adhesion Antibodies, Monoclonal - immunology Antigens, Neoplasm - blood Biomarkers, Tumor - blood Breast cancer Breast Neoplasms - blood Breast Neoplasms - drug therapy Breast Neoplasms - metabolism Breast Neoplasms - pathology Cell Adhesion Molecules - blood Cell Count Cell Membrane - metabolism Epithelial Cell Adhesion Molecule Epithelial-Mesenchymal Transition - drug effects Female Humans Medical research Metastasis Methods Monitoring methods Neoplasm Metastasis Neoplastic Cells, Circulating - drug effects Neoplastic Cells, Circulating - pathology Patients Pilot Projects Retrospective Studies Sarcoma Studies Treatment Outcome Vimentin - immunology Vimentin - metabolism |
| title | Circulating tumor cell enumeration with a combination of epithelial cell adhesion molecule- and cell-surface vimentin-based methods for monitoring breast cancer therapeutic response |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T01%3A52%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Circulating%20tumor%20cell%20enumeration%20with%20a%20combination%20of%20epithelial%20cell%20adhesion%20molecule-%20and%20cell-surface%20vimentin-based%20methods%20for%20monitoring%20breast%20cancer%20therapeutic%20response&rft.jtitle=Clinical%20chemistry%20(Baltimore,%20Md.)&rft.au=Satelli,%20Arun&rft.date=2015-01&rft.volume=61&rft.issue=1&rft.spage=259&rft.epage=266&rft.pages=259-266&rft.issn=0009-9147&rft.eissn=1530-8561&rft_id=info:doi/10.1373/clinchem.2014.228122&rft_dat=%3Cproquest_cross%3E1641427197%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1661579560&rft_id=info:pmid/25336717&rfr_iscdi=true |