Expression of glucoamylase gene using SUC2 promoter in Saccharomyces cerevisiae
The cloning of glucoamylase gene STA using the SUC2 promoter into Saccharomyces cerevisiae was performed. The signal sequence of STA gene was used for the secretion of glucoamylase protein. The plasmid constructed in this way was named YEpSUCSTA and its expression was identified. The expression of Y...
Gespeichert in:
| Veröffentlicht in: | Biotechnology letters 1992-09, Vol.14 (9), p.747-752 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 752 |
|---|---|
| container_issue | 9 |
| container_start_page | 747 |
| container_title | Biotechnology letters |
| container_volume | 14 |
| creator | Cha, H.J Yoo, Y.J Ahn, J.H Kang, H.S |
| description | The cloning of glucoamylase gene STA using the SUC2 promoter into Saccharomyces cerevisiae was performed. The signal sequence of STA gene was used for the secretion of glucoamylase protein. The plasmid constructed in this way was named YEpSUCSTA and its expression was identified. The expression of YEpSUCSTA was repressed in the presence of glucose in growth medium, but derepressed when glucose became depleted. YEpSUCSTA showed the similar efficiency of glucoamylase secretion as YEpSTA-F which has the entire STA gene. Glucoamylase activity in starch-glucose medium was largely increased because cell mass and plasmid stability were high in biosynthesis phase compared to extracellular glucoamylase activities in media which starch or glucose was the only carbon source. |
| doi_str_mv | 10.1007/BF01029132 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pasca</sourceid><recordid>TN_cdi_proquest_miscellaneous_16407580</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>16407580</sourcerecordid><originalsourceid>FETCH-LOGICAL-f237t-1a4cddaf858d514b2ba1fc7eeaee89599b34a2ddf98e45f365adf570b60fb4a13</originalsourceid><addsrcrecordid>eNotzl9LwzAUBfAgCs7pi1_APIhv1ZsmaZpHHZsKgz3MPZfb9KZW-mcmq7hvb2E-HTj8OBzGbgU8CgDz9LICAakVMj1jM6GNTDJjsnM2A6FEopVNL9lVjF8AYA2YGdssf_eBYmyGng-e1-3oBuyOLUbiNfXEx9j0Nd_uFinfh6EbDhR40_MtOveJU3F0FLmjQD9NbJCu2YXHNtLNf87ZbrX8WLwl683r--J5nfhUmkMiULmqQp_rvNJClWmJwjtDhES51daWUmFaVd7mpLSXmcbKawNlBr5UKOScPZx2p1PfI8VD0TXRUdtiT8MYC5EpMDqHCd7_Q4wOWx-wd00s9qHpMBwLpaQxwk7s7sQ8DgXWYSK7bQpCgjBZZiXIP0rxaDY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16407580</pqid></control><display><type>article</type><title>Expression of glucoamylase gene using SUC2 promoter in Saccharomyces cerevisiae</title><source>Springer Nature - Complete Springer Journals</source><creator>Cha, H.J ; Yoo, Y.J ; Ahn, J.H ; Kang, H.S</creator><creatorcontrib>Cha, H.J ; Yoo, Y.J ; Ahn, J.H ; Kang, H.S</creatorcontrib><description>The cloning of glucoamylase gene STA using the SUC2 promoter into Saccharomyces cerevisiae was performed. The signal sequence of STA gene was used for the secretion of glucoamylase protein. The plasmid constructed in this way was named YEpSUCSTA and its expression was identified. The expression of YEpSUCSTA was repressed in the presence of glucose in growth medium, but derepressed when glucose became depleted. YEpSUCSTA showed the similar efficiency of glucoamylase secretion as YEpSTA-F which has the entire STA gene. Glucoamylase activity in starch-glucose medium was largely increased because cell mass and plasmid stability were high in biosynthesis phase compared to extracellular glucoamylase activities in media which starch or glucose was the only carbon source.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/BF01029132</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Biological and medical sciences ; biosynthesis ; Biotechnology ; chimeric plasmids ; enzyme activity ; Fundamental and applied biological sciences. Psychology ; gene expression ; Genetic engineering ; genetic recombination ; Genetic technics ; glucan 1,4-alpha-glucosidase ; Methods. Procedures. Technologies ; plasmids ; promoter regions ; protein secretion ; Saccharomyces cerevisiae ; structural genes ; Vectors (cloning, transfer, expression). Insertion sequences and transposons</subject><ispartof>Biotechnology letters, 1992-09, Vol.14 (9), p.747-752</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4437719$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Cha, H.J</creatorcontrib><creatorcontrib>Yoo, Y.J</creatorcontrib><creatorcontrib>Ahn, J.H</creatorcontrib><creatorcontrib>Kang, H.S</creatorcontrib><title>Expression of glucoamylase gene using SUC2 promoter in Saccharomyces cerevisiae</title><title>Biotechnology letters</title><description>The cloning of glucoamylase gene STA using the SUC2 promoter into Saccharomyces cerevisiae was performed. The signal sequence of STA gene was used for the secretion of glucoamylase protein. The plasmid constructed in this way was named YEpSUCSTA and its expression was identified. The expression of YEpSUCSTA was repressed in the presence of glucose in growth medium, but derepressed when glucose became depleted. YEpSUCSTA showed the similar efficiency of glucoamylase secretion as YEpSTA-F which has the entire STA gene. Glucoamylase activity in starch-glucose medium was largely increased because cell mass and plasmid stability were high in biosynthesis phase compared to extracellular glucoamylase activities in media which starch or glucose was the only carbon source.</description><subject>Biological and medical sciences</subject><subject>biosynthesis</subject><subject>Biotechnology</subject><subject>chimeric plasmids</subject><subject>enzyme activity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>Genetic engineering</subject><subject>genetic recombination</subject><subject>Genetic technics</subject><subject>glucan 1,4-alpha-glucosidase</subject><subject>Methods. Procedures. Technologies</subject><subject>plasmids</subject><subject>promoter regions</subject><subject>protein secretion</subject><subject>Saccharomyces cerevisiae</subject><subject>structural genes</subject><subject>Vectors (cloning, transfer, expression). Insertion sequences and transposons</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNotzl9LwzAUBfAgCs7pi1_APIhv1ZsmaZpHHZsKgz3MPZfb9KZW-mcmq7hvb2E-HTj8OBzGbgU8CgDz9LICAakVMj1jM6GNTDJjsnM2A6FEopVNL9lVjF8AYA2YGdssf_eBYmyGng-e1-3oBuyOLUbiNfXEx9j0Nd_uFinfh6EbDhR40_MtOveJU3F0FLmjQD9NbJCu2YXHNtLNf87ZbrX8WLwl683r--J5nfhUmkMiULmqQp_rvNJClWmJwjtDhES51daWUmFaVd7mpLSXmcbKawNlBr5UKOScPZx2p1PfI8VD0TXRUdtiT8MYC5EpMDqHCd7_Q4wOWx-wd00s9qHpMBwLpaQxwk7s7sQ8DgXWYSK7bQpCgjBZZiXIP0rxaDY</recordid><startdate>19920901</startdate><enddate>19920901</enddate><creator>Cha, H.J</creator><creator>Yoo, Y.J</creator><creator>Ahn, J.H</creator><creator>Kang, H.S</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>19920901</creationdate><title>Expression of glucoamylase gene using SUC2 promoter in Saccharomyces cerevisiae</title><author>Cha, H.J ; Yoo, Y.J ; Ahn, J.H ; Kang, H.S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f237t-1a4cddaf858d514b2ba1fc7eeaee89599b34a2ddf98e45f365adf570b60fb4a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Biological and medical sciences</topic><topic>biosynthesis</topic><topic>Biotechnology</topic><topic>chimeric plasmids</topic><topic>enzyme activity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>Genetic engineering</topic><topic>genetic recombination</topic><topic>Genetic technics</topic><topic>glucan 1,4-alpha-glucosidase</topic><topic>Methods. Procedures. Technologies</topic><topic>plasmids</topic><topic>promoter regions</topic><topic>protein secretion</topic><topic>Saccharomyces cerevisiae</topic><topic>structural genes</topic><topic>Vectors (cloning, transfer, expression). Insertion sequences and transposons</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cha, H.J</creatorcontrib><creatorcontrib>Yoo, Y.J</creatorcontrib><creatorcontrib>Ahn, J.H</creatorcontrib><creatorcontrib>Kang, H.S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biotechnology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cha, H.J</au><au>Yoo, Y.J</au><au>Ahn, J.H</au><au>Kang, H.S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of glucoamylase gene using SUC2 promoter in Saccharomyces cerevisiae</atitle><jtitle>Biotechnology letters</jtitle><date>1992-09-01</date><risdate>1992</risdate><volume>14</volume><issue>9</issue><spage>747</spage><epage>752</epage><pages>747-752</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><coden>BILED3</coden><abstract>The cloning of glucoamylase gene STA using the SUC2 promoter into Saccharomyces cerevisiae was performed. The signal sequence of STA gene was used for the secretion of glucoamylase protein. The plasmid constructed in this way was named YEpSUCSTA and its expression was identified. The expression of YEpSUCSTA was repressed in the presence of glucose in growth medium, but derepressed when glucose became depleted. YEpSUCSTA showed the similar efficiency of glucoamylase secretion as YEpSTA-F which has the entire STA gene. Glucoamylase activity in starch-glucose medium was largely increased because cell mass and plasmid stability were high in biosynthesis phase compared to extracellular glucoamylase activities in media which starch or glucose was the only carbon source.</abstract><cop>Dordrecht</cop><pub>Springer</pub><doi>10.1007/BF01029132</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0141-5492 |
| ispartof | Biotechnology letters, 1992-09, Vol.14 (9), p.747-752 |
| issn | 0141-5492 1573-6776 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16407580 |
| source | Springer Nature - Complete Springer Journals |
| subjects | Biological and medical sciences biosynthesis Biotechnology chimeric plasmids enzyme activity Fundamental and applied biological sciences. Psychology gene expression Genetic engineering genetic recombination Genetic technics glucan 1,4-alpha-glucosidase Methods. Procedures. Technologies plasmids promoter regions protein secretion Saccharomyces cerevisiae structural genes Vectors (cloning, transfer, expression). Insertion sequences and transposons |
| title | Expression of glucoamylase gene using SUC2 promoter in Saccharomyces cerevisiae |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T13%3A56%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pasca&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20glucoamylase%20gene%20using%20SUC2%20promoter%20in%20Saccharomyces%20cerevisiae&rft.jtitle=Biotechnology%20letters&rft.au=Cha,%20H.J&rft.date=1992-09-01&rft.volume=14&rft.issue=9&rft.spage=747&rft.epage=752&rft.pages=747-752&rft.issn=0141-5492&rft.eissn=1573-6776&rft.coden=BILED3&rft_id=info:doi/10.1007/BF01029132&rft_dat=%3Cproquest_pasca%3E16407580%3C/proquest_pasca%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16407580&rft_id=info:pmid/&rfr_iscdi=true |