Isolation of Contractile Cardiomyocytes from Human Pluripotent Stem-Cell-Derived Cardiomyogenic Cultures Using a Human NCX1-EGFP Reporter

The prospective isolation of defined contractile human pluripotent stem cell (hPSC)–derived cardiomyocytes is advantageous for regenerative medicine and drug screening applications. Currently, enrichment of cardiomyocyte populations from such cultures can be achieved by combinations of cell surface...

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Veröffentlicht in:	Stem cells and development 2015-01, Vol.24 (1), p.11-20
Hauptverfasser:	Ovchinnikov, Dmitry A., Hidalgo, Alejandro, Yang, Seung-Kwon, Zhang, Xinli, Hudson, James, Mazzone, Stuart B., Chen, Chen, Cooper-White, Justin J., Wolvetang, Ernst J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Culture Techniques Cell Line Flow Cytometry Genes, Reporter Green Fluorescent Proteins - biosynthesis Green Fluorescent Proteins - genetics Humans Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - metabolism Myocardial Contraction Myocytes, Cardiac - cytology Myocytes, Cardiac - metabolism Original Research Reports Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - genetics Sodium-Calcium Exchanger - biosynthesis Sodium-Calcium Exchanger - genetics
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