A close connection between the PERK and IRE arms of the UPR and the transcriptional regulation of autophagy
•Microarray of HCT116 cells.•Autophagy genes upregulated.•PERK and IRE1 important for regulation. Endoplasmic reticulum (ER) stress is known to lead to activation of both the unfolded protein response (UPR) and autophagy. Although regulatory connections have been identified between the UPR and autop...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2015-01, Vol.456 (1), p.305-311 |
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| creator | Deegan, Shane Koryga, Izabela Glynn, Sharon A. Gupta, Sanjeev Gorman, Adrienne M. Samali, Afshin |
| description | •Microarray of HCT116 cells.•Autophagy genes upregulated.•PERK and IRE1 important for regulation.
Endoplasmic reticulum (ER) stress is known to lead to activation of both the unfolded protein response (UPR) and autophagy. Although regulatory connections have been identified between the UPR and autophagy, it is still unclear to what extent the UPR regulates the genes involved at the different stages of the autophagy pathway. Here, we carried out a microarray analysis of HCT116 cells subjected to ER stress and observed the transcriptional upregulation of a large cohort of autophagy-related genes. Of particular interest, we identified the transcriptional upregulation of the autophagy receptor genes SQSTM1/p62, NBR1 and BNIP3L/NIX in response to ER stress and show that the inhibition of the UPR transmembrane receptors, PERK and IRE1, abrogates this upregulation. |
| doi_str_mv | 10.1016/j.bbrc.2014.11.076 |
| format | Article |
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Endoplasmic reticulum (ER) stress is known to lead to activation of both the unfolded protein response (UPR) and autophagy. Although regulatory connections have been identified between the UPR and autophagy, it is still unclear to what extent the UPR regulates the genes involved at the different stages of the autophagy pathway. Here, we carried out a microarray analysis of HCT116 cells subjected to ER stress and observed the transcriptional upregulation of a large cohort of autophagy-related genes. Of particular interest, we identified the transcriptional upregulation of the autophagy receptor genes SQSTM1/p62, NBR1 and BNIP3L/NIX in response to ER stress and show that the inhibition of the UPR transmembrane receptors, PERK and IRE1, abrogates this upregulation.</description><subject>Adaptor Proteins, Signal Transducing - metabolism</subject><subject>Autophagy</subject><subject>Autophagy - genetics</subject><subject>DNA, Complementary - metabolism</subject><subject>eIF-2 Kinase - metabolism</subject><subject>Endoplasmic Reticulum Stress</subject><subject>Endoribonucleases - metabolism</subject><subject>ER stress</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>HCT116 Cells</subject><subject>Humans</subject><subject>IRE1</subject><subject>Membrane Proteins - metabolism</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>PERK</subject><subject>Protein-Serine-Threonine Kinases - metabolism</subject><subject>Proteins - metabolism</subject><subject>Proto-Oncogene Proteins - metabolism</subject><subject>Sequestosome-1 Protein</subject><subject>Tumor Suppressor Proteins - metabolism</subject><subject>Unfolded Protein Response</subject><subject>Unfolded protein response (UPR)</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1P3DAQhi0Egi3wB3qofOSSdMYfyUbqBaEtRUUCrUDiZjnOBLLNxls7odp_T7JLOXIaa_y8rzQPY18RUgTMvq_SsgwuFYAqRUwhzw7YDKGARCCoQzYDgCwRBT6dsC8xrgAQVVYcsxOhVa5zLGbszyV3rY_Ene86cn3jO15S_4-o4_0L8fvF8je3XcVvlgtuwzpyX-8-Hu-Xu_307oPtogvNZorblgd6Hlq76xppO_R-82Kft2fsqLZtpPP3ecoefy4ern4lt3fXN1eXt4lTheiTGjTMXZELJJm7WmZElSw0aKnneeWk1lUpMykKlWOpBIoSc5QZVqTBOiXkKbvY926C_ztQ7M26iY7a1nbkh2gwU6DmAhFHVOxRF3yMgWqzCc3ahq1BMJNkszKTZDNJNohmlDyGvr33D-Waqo_If6sj8GMP0Hjla0PBRNdQ56hqwujYVL75rP8NSwCMEA</recordid><startdate>20150102</startdate><enddate>20150102</enddate><creator>Deegan, Shane</creator><creator>Koryga, Izabela</creator><creator>Glynn, Sharon A.</creator><creator>Gupta, Sanjeev</creator><creator>Gorman, Adrienne M.</creator><creator>Samali, Afshin</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150102</creationdate><title>A close connection between the PERK and IRE arms of the UPR and the transcriptional regulation of autophagy</title><author>Deegan, Shane ; Koryga, Izabela ; Glynn, Sharon A. ; Gupta, Sanjeev ; Gorman, Adrienne M. ; Samali, Afshin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c492t-f0508c9721e37cf36eed395053587dc355db36329471b4212b171361de50ac423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adaptor Proteins, Signal Transducing - metabolism</topic><topic>Autophagy</topic><topic>Autophagy - genetics</topic><topic>DNA, Complementary - metabolism</topic><topic>eIF-2 Kinase - metabolism</topic><topic>Endoplasmic Reticulum Stress</topic><topic>Endoribonucleases - metabolism</topic><topic>ER stress</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>HCT116 Cells</topic><topic>Humans</topic><topic>IRE1</topic><topic>Membrane Proteins - metabolism</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>PERK</topic><topic>Protein-Serine-Threonine Kinases - metabolism</topic><topic>Proteins - metabolism</topic><topic>Proto-Oncogene Proteins - metabolism</topic><topic>Sequestosome-1 Protein</topic><topic>Tumor Suppressor Proteins - metabolism</topic><topic>Unfolded Protein Response</topic><topic>Unfolded protein response (UPR)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deegan, Shane</creatorcontrib><creatorcontrib>Koryga, Izabela</creatorcontrib><creatorcontrib>Glynn, Sharon A.</creatorcontrib><creatorcontrib>Gupta, Sanjeev</creatorcontrib><creatorcontrib>Gorman, Adrienne M.</creatorcontrib><creatorcontrib>Samali, Afshin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deegan, Shane</au><au>Koryga, Izabela</au><au>Glynn, Sharon A.</au><au>Gupta, Sanjeev</au><au>Gorman, Adrienne M.</au><au>Samali, Afshin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A close connection between the PERK and IRE arms of the UPR and the transcriptional regulation of autophagy</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2015-01-02</date><risdate>2015</risdate><volume>456</volume><issue>1</issue><spage>305</spage><epage>311</epage><pages>305-311</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>•Microarray of HCT116 cells.•Autophagy genes upregulated.•PERK and IRE1 important for regulation.
Endoplasmic reticulum (ER) stress is known to lead to activation of both the unfolded protein response (UPR) and autophagy. Although regulatory connections have been identified between the UPR and autophagy, it is still unclear to what extent the UPR regulates the genes involved at the different stages of the autophagy pathway. Here, we carried out a microarray analysis of HCT116 cells subjected to ER stress and observed the transcriptional upregulation of a large cohort of autophagy-related genes. Of particular interest, we identified the transcriptional upregulation of the autophagy receptor genes SQSTM1/p62, NBR1 and BNIP3L/NIX in response to ER stress and show that the inhibition of the UPR transmembrane receptors, PERK and IRE1, abrogates this upregulation.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>25475719</pmid><doi>10.1016/j.bbrc.2014.11.076</doi><tpages>7</tpages></addata></record> |
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| subjects | Adaptor Proteins, Signal Transducing - metabolism Autophagy Autophagy - genetics DNA, Complementary - metabolism eIF-2 Kinase - metabolism Endoplasmic Reticulum Stress Endoribonucleases - metabolism ER stress Gene Expression Profiling Gene Expression Regulation HCT116 Cells Humans IRE1 Membrane Proteins - metabolism Oligonucleotide Array Sequence Analysis PERK Protein-Serine-Threonine Kinases - metabolism Proteins - metabolism Proto-Oncogene Proteins - metabolism Sequestosome-1 Protein Tumor Suppressor Proteins - metabolism Unfolded Protein Response Unfolded protein response (UPR) |
| title | A close connection between the PERK and IRE arms of the UPR and the transcriptional regulation of autophagy |
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