Identification of a natural intergenotypic recombinant hepatitis delta virus genotype 1 and 2 in Vietnamese HBsAg-positive patients
Summary Hepatitis D virus (HDV) infection is acquired as a co‐ /superinfection of Hepatitis B virus (HBV) and can modulate the pathophysiology of chronic hepatitis B and related liver diseases including hepatocellular carcinoma. Among the eight distinct HDV genotypes reported, relatively few studies...
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| Veröffentlicht in: | Journal of viral hepatitis 2015-01, Vol.22 (1), p.55-63 |
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| creator | Sy, B. T. Nguyen, H. M. Toan, N. L. Song, L. H. Tong, H. V. Wolboldt, C. Binh, V. Q. Kremsner, P. G. Velavan, T. P. Bock, C.-T. |
| description | Summary
Hepatitis D virus (HDV) infection is acquired as a co‐ /superinfection of Hepatitis B virus (HBV) and can modulate the pathophysiology of chronic hepatitis B and related liver diseases including hepatocellular carcinoma. Among the eight distinct HDV genotypes reported, relatively few studies have attempted to investigate the prevalence of HDV mixed genotypes and RNA recombination of HDV. With a recorded prevalence of 10–20% HBV infection in Vietnam, this study investigated the HDV variability, HDV genotypes and HDV recombination among twenty‐one HDV isolates in Vietnamese HBsAg‐positive patients. HDV subgenomic and full‐length genome sequences were obtained using newly established HDV‐specific RT‐PCR techniques. The nucleotide homology was observed from 74.6% to 99.4% among the investigated full‐length genome of the HDV isolates. We observed HDV genotype 1 and HDV genotype 2 in the investigated Vietnamese patients. Although no HDV genotype mixtures were observed, we report here a newly identified recombinant of HDV genotypes (HDV 1 and HDV 2). The identified recombinant HDV isolate C03 revealed sequence homology to both HDV genotype 1 (nt1 to nt907) and HDV genotype 2 (nt908 to nt1675; HDAg coding region) with a breakpoint at nt908. Our findings demonstrate the prevalence of intergenotypic recombination between HDV genotypes 1 and 2 in a Vietnamese HBsAg‐positive patient. Extended investigation on the distribution and prevalence of HDV, HDV mixed genotypes and recombinant HDV genotypes in a larger Vietnamese population offers vital insights into understanding of the micro‐epidemiology of HDV and subsequent pathophysiology in chronic HBV‐ /HDV‐related liver diseases. |
| doi_str_mv | 10.1111/jvh.12228 |
| format | Article |
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Hepatitis D virus (HDV) infection is acquired as a co‐ /superinfection of Hepatitis B virus (HBV) and can modulate the pathophysiology of chronic hepatitis B and related liver diseases including hepatocellular carcinoma. Among the eight distinct HDV genotypes reported, relatively few studies have attempted to investigate the prevalence of HDV mixed genotypes and RNA recombination of HDV. With a recorded prevalence of 10–20% HBV infection in Vietnam, this study investigated the HDV variability, HDV genotypes and HDV recombination among twenty‐one HDV isolates in Vietnamese HBsAg‐positive patients. HDV subgenomic and full‐length genome sequences were obtained using newly established HDV‐specific RT‐PCR techniques. The nucleotide homology was observed from 74.6% to 99.4% among the investigated full‐length genome of the HDV isolates. We observed HDV genotype 1 and HDV genotype 2 in the investigated Vietnamese patients. Although no HDV genotype mixtures were observed, we report here a newly identified recombinant of HDV genotypes (HDV 1 and HDV 2). The identified recombinant HDV isolate C03 revealed sequence homology to both HDV genotype 1 (nt1 to nt907) and HDV genotype 2 (nt908 to nt1675; HDAg coding region) with a breakpoint at nt908. Our findings demonstrate the prevalence of intergenotypic recombination between HDV genotypes 1 and 2 in a Vietnamese HBsAg‐positive patient. Extended investigation on the distribution and prevalence of HDV, HDV mixed genotypes and recombinant HDV genotypes in a larger Vietnamese population offers vital insights into understanding of the micro‐epidemiology of HDV and subsequent pathophysiology in chronic HBV‐ /HDV‐related liver diseases.</description><identifier>ISSN: 1352-0504</identifier><identifier>EISSN: 1365-2893</identifier><identifier>DOI: 10.1111/jvh.12228</identifier><identifier>PMID: 24548489</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Adult ; Aged ; Asian Continental Ancestry Group ; Female ; Genetic Variation ; Genomes ; Genotype ; Genotype & phenotype ; HDV genotype ; HDV genotype mixture ; HDV recombinant ; Hepatitis ; Hepatitis B - complications ; Hepatitis B Surface Antigens - blood ; Hepatitis D - virology ; Hepatitis D virus ; Hepatitis Delta Virus - classification ; Hepatitis Delta Virus - genetics ; Hepatitis Delta Virus - isolation & purification ; Humans ; Liver diseases ; Male ; Middle Aged ; Molecular Sequence Data ; phylogenetic analysis ; Phylogeny ; Recombination, Genetic ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Viral - genetics ; Sequence Analysis, DNA ; Sequence Homology ; Studies ; variability ; Young Adult</subject><ispartof>Journal of viral hepatitis, 2015-01, Vol.22 (1), p.55-63</ispartof><rights>2014 John Wiley & Sons Ltd</rights><rights>2014 John Wiley & Sons Ltd.</rights><rights>Copyright © 2015 John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjvh.12228$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjvh.12228$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24548489$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sy, B. T.</creatorcontrib><creatorcontrib>Nguyen, H. M.</creatorcontrib><creatorcontrib>Toan, N. L.</creatorcontrib><creatorcontrib>Song, L. H.</creatorcontrib><creatorcontrib>Tong, H. V.</creatorcontrib><creatorcontrib>Wolboldt, C.</creatorcontrib><creatorcontrib>Binh, V. Q.</creatorcontrib><creatorcontrib>Kremsner, P. G.</creatorcontrib><creatorcontrib>Velavan, T. P.</creatorcontrib><creatorcontrib>Bock, C.-T.</creatorcontrib><title>Identification of a natural intergenotypic recombinant hepatitis delta virus genotype 1 and 2 in Vietnamese HBsAg-positive patients</title><title>Journal of viral hepatitis</title><addtitle>J Viral Hepat</addtitle><description>Summary
Hepatitis D virus (HDV) infection is acquired as a co‐ /superinfection of Hepatitis B virus (HBV) and can modulate the pathophysiology of chronic hepatitis B and related liver diseases including hepatocellular carcinoma. Among the eight distinct HDV genotypes reported, relatively few studies have attempted to investigate the prevalence of HDV mixed genotypes and RNA recombination of HDV. With a recorded prevalence of 10–20% HBV infection in Vietnam, this study investigated the HDV variability, HDV genotypes and HDV recombination among twenty‐one HDV isolates in Vietnamese HBsAg‐positive patients. HDV subgenomic and full‐length genome sequences were obtained using newly established HDV‐specific RT‐PCR techniques. The nucleotide homology was observed from 74.6% to 99.4% among the investigated full‐length genome of the HDV isolates. We observed HDV genotype 1 and HDV genotype 2 in the investigated Vietnamese patients. Although no HDV genotype mixtures were observed, we report here a newly identified recombinant of HDV genotypes (HDV 1 and HDV 2). The identified recombinant HDV isolate C03 revealed sequence homology to both HDV genotype 1 (nt1 to nt907) and HDV genotype 2 (nt908 to nt1675; HDAg coding region) with a breakpoint at nt908. Our findings demonstrate the prevalence of intergenotypic recombination between HDV genotypes 1 and 2 in a Vietnamese HBsAg‐positive patient. Extended investigation on the distribution and prevalence of HDV, HDV mixed genotypes and recombinant HDV genotypes in a larger Vietnamese population offers vital insights into understanding of the micro‐epidemiology of HDV and subsequent pathophysiology in chronic HBV‐ /HDV‐related liver diseases.</description><subject>Adult</subject><subject>Aged</subject><subject>Asian Continental Ancestry Group</subject><subject>Female</subject><subject>Genetic Variation</subject><subject>Genomes</subject><subject>Genotype</subject><subject>Genotype & phenotype</subject><subject>HDV genotype</subject><subject>HDV genotype mixture</subject><subject>HDV recombinant</subject><subject>Hepatitis</subject><subject>Hepatitis B - complications</subject><subject>Hepatitis B Surface Antigens - blood</subject><subject>Hepatitis D - virology</subject><subject>Hepatitis D virus</subject><subject>Hepatitis Delta Virus - classification</subject><subject>Hepatitis Delta Virus - genetics</subject><subject>Hepatitis Delta Virus - isolation & purification</subject><subject>Humans</subject><subject>Liver diseases</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>phylogenetic analysis</subject><subject>Phylogeny</subject><subject>Recombination, Genetic</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Viral - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology</subject><subject>Studies</subject><subject>variability</subject><subject>Young Adult</subject><issn>1352-0504</issn><issn>1365-2893</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc1O3DAUha2qqPy0i74AssSmm4B_E2cJqJ2hjGgXLZW6sZzkBjwkTmo7A7Pmxethpizqja_k7zu2fBD6SMkpTetsubo_pYwx9QYdUJ7LjKmSv93MkmVEErGPDkNYEkI5k_Qd2mdCCiVUeYCerxpw0ba2NtEODg8tNtiZOHnTYesi-DtwQ1yPtsYe6qGvrDMu4nsYkxBtwA100eCV9VPAOxYwxcY1mKUEfGshOtNDADy_COd32TiEJK4AbxLS5eE92mtNF-DDbj9CP798_nE5zxbfZleX54vMclWoDGQjgFHB6rJUhufQMAZcgRFVzpkylDQKWqgMMRWRsoJClaxULQBvi5bl_Ah92uaOfvgzQYi6t6GGrjMOhilomgsiFJFUJvTkP3Q5TN6l1yWKl0LJxCXqeEdNVQ-NHr3tjV_rf9-bgLMt8Gg7WL-eU6I3venUm37pTX-9nb8Myci2hg0Rnl4N4x90XvBC6l83My1mYnHz_TfV1_wvZ9-a8Q</recordid><startdate>201501</startdate><enddate>201501</enddate><creator>Sy, B. T.</creator><creator>Nguyen, H. M.</creator><creator>Toan, N. L.</creator><creator>Song, L. H.</creator><creator>Tong, H. V.</creator><creator>Wolboldt, C.</creator><creator>Binh, V. Q.</creator><creator>Kremsner, P. G.</creator><creator>Velavan, T. P.</creator><creator>Bock, C.-T.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>201501</creationdate><title>Identification of a natural intergenotypic recombinant hepatitis delta virus genotype 1 and 2 in Vietnamese HBsAg-positive patients</title><author>Sy, B. T. ; Nguyen, H. M. ; Toan, N. L. ; Song, L. H. ; Tong, H. V. ; Wolboldt, C. ; Binh, V. Q. ; Kremsner, P. G. ; Velavan, T. P. ; Bock, C.-T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i3878-e5d4e2142c998a36ed22e38ea4b6328a10d8efeba0ab055be789298fee3f7f263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Asian Continental Ancestry Group</topic><topic>Female</topic><topic>Genetic Variation</topic><topic>Genomes</topic><topic>Genotype</topic><topic>Genotype & phenotype</topic><topic>HDV genotype</topic><topic>HDV genotype mixture</topic><topic>HDV recombinant</topic><topic>Hepatitis</topic><topic>Hepatitis B - complications</topic><topic>Hepatitis B Surface Antigens - blood</topic><topic>Hepatitis D - virology</topic><topic>Hepatitis D virus</topic><topic>Hepatitis Delta Virus - classification</topic><topic>Hepatitis Delta Virus - genetics</topic><topic>Hepatitis Delta Virus - isolation & purification</topic><topic>Humans</topic><topic>Liver diseases</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Molecular Sequence Data</topic><topic>phylogenetic analysis</topic><topic>Phylogeny</topic><topic>Recombination, Genetic</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Viral - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology</topic><topic>Studies</topic><topic>variability</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sy, B. T.</creatorcontrib><creatorcontrib>Nguyen, H. M.</creatorcontrib><creatorcontrib>Toan, N. L.</creatorcontrib><creatorcontrib>Song, L. H.</creatorcontrib><creatorcontrib>Tong, H. V.</creatorcontrib><creatorcontrib>Wolboldt, C.</creatorcontrib><creatorcontrib>Binh, V. Q.</creatorcontrib><creatorcontrib>Kremsner, P. G.</creatorcontrib><creatorcontrib>Velavan, T. P.</creatorcontrib><creatorcontrib>Bock, C.-T.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of viral hepatitis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sy, B. T.</au><au>Nguyen, H. M.</au><au>Toan, N. L.</au><au>Song, L. H.</au><au>Tong, H. V.</au><au>Wolboldt, C.</au><au>Binh, V. Q.</au><au>Kremsner, P. G.</au><au>Velavan, T. P.</au><au>Bock, C.-T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of a natural intergenotypic recombinant hepatitis delta virus genotype 1 and 2 in Vietnamese HBsAg-positive patients</atitle><jtitle>Journal of viral hepatitis</jtitle><addtitle>J Viral Hepat</addtitle><date>2015-01</date><risdate>2015</risdate><volume>22</volume><issue>1</issue><spage>55</spage><epage>63</epage><pages>55-63</pages><issn>1352-0504</issn><eissn>1365-2893</eissn><abstract>Summary
Hepatitis D virus (HDV) infection is acquired as a co‐ /superinfection of Hepatitis B virus (HBV) and can modulate the pathophysiology of chronic hepatitis B and related liver diseases including hepatocellular carcinoma. Among the eight distinct HDV genotypes reported, relatively few studies have attempted to investigate the prevalence of HDV mixed genotypes and RNA recombination of HDV. With a recorded prevalence of 10–20% HBV infection in Vietnam, this study investigated the HDV variability, HDV genotypes and HDV recombination among twenty‐one HDV isolates in Vietnamese HBsAg‐positive patients. HDV subgenomic and full‐length genome sequences were obtained using newly established HDV‐specific RT‐PCR techniques. The nucleotide homology was observed from 74.6% to 99.4% among the investigated full‐length genome of the HDV isolates. We observed HDV genotype 1 and HDV genotype 2 in the investigated Vietnamese patients. Although no HDV genotype mixtures were observed, we report here a newly identified recombinant of HDV genotypes (HDV 1 and HDV 2). The identified recombinant HDV isolate C03 revealed sequence homology to both HDV genotype 1 (nt1 to nt907) and HDV genotype 2 (nt908 to nt1675; HDAg coding region) with a breakpoint at nt908. Our findings demonstrate the prevalence of intergenotypic recombination between HDV genotypes 1 and 2 in a Vietnamese HBsAg‐positive patient. Extended investigation on the distribution and prevalence of HDV, HDV mixed genotypes and recombinant HDV genotypes in a larger Vietnamese population offers vital insights into understanding of the micro‐epidemiology of HDV and subsequent pathophysiology in chronic HBV‐ /HDV‐related liver diseases.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>24548489</pmid><doi>10.1111/jvh.12228</doi><tpages>9</tpages></addata></record> |
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| subjects | Adult Aged Asian Continental Ancestry Group Female Genetic Variation Genomes Genotype Genotype & phenotype HDV genotype HDV genotype mixture HDV recombinant Hepatitis Hepatitis B - complications Hepatitis B Surface Antigens - blood Hepatitis D - virology Hepatitis D virus Hepatitis Delta Virus - classification Hepatitis Delta Virus - genetics Hepatitis Delta Virus - isolation & purification Humans Liver diseases Male Middle Aged Molecular Sequence Data phylogenetic analysis Phylogeny Recombination, Genetic Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - genetics Sequence Analysis, DNA Sequence Homology Studies variability Young Adult |
| title | Identification of a natural intergenotypic recombinant hepatitis delta virus genotype 1 and 2 in Vietnamese HBsAg-positive patients |
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