Myoblast-Mediated Gene Therapy Improves Functional Collateralization in Chronic Cerebral Hypoperfusion
BACKGROUND AND PURPOSE—Direct extracranial–intracranial bypass surgery for treatment of cerebral hemodynamic compromise remains hindered by complications but alternative simple and safe indirect revascularization procedures, such as an encephalomyosynangiosis (EMS), lack hemodynamic efficiency. Here...
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| Veröffentlicht in: | Stroke (1970) 2015-01, Vol.46 (1), p.203-211 |
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| container_title | Stroke (1970) |
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| creator | Hecht, Nils Marushima, Aiki Nieminen, Melina Kremenetskaia, Irina von Degenfeld, Georges Woitzik, Johannes Vajkoczy, Peter |
| description | BACKGROUND AND PURPOSE—Direct extracranial–intracranial bypass surgery for treatment of cerebral hemodynamic compromise remains hindered by complications but alternative simple and safe indirect revascularization procedures, such as an encephalomyosynangiosis (EMS), lack hemodynamic efficiency. Here, the myoblast-mediated transfer of angiogenic genes presents an approach for induction of therapeutic collateralization. In this study, we tested the effect of myoblast-mediated delivery of vascular endothelial growth factor-A (VEGF) to the muscle/brain interface of an EMS in a model of chronic cerebral hypoperfusion.
METHODS—Permanent unilateral internal carotid artery-occlusion was performed in adult C57/BL6 mice with or without (no EMS) surgical grafting of an EMS followed by implantation of monoclonal mouse myoblasts expressing either VEGF164 or an empty vector (EV). Cerebral hemodynamic impairment, transpial collateralization, angiogenesis, mural cell investment, microvascular permeability, and cortical infarction after ipsilateral stroke were assessed by real-time laser speckle blood flow imaging, 2- and 3-dimensional immunofluorescence and MRI.
RESULTS—VEGF-expressing myoblasts improved hemodynamic rescue by day 14 (no EMS 37±21%, EV 42±9%, VEGF 48±12%; P |
| doi_str_mv | 10.1161/STROKEAHA.114.006712 |
| format | Article |
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METHODS—Permanent unilateral internal carotid artery-occlusion was performed in adult C57/BL6 mice with or without (no EMS) surgical grafting of an EMS followed by implantation of monoclonal mouse myoblasts expressing either VEGF164 or an empty vector (EV). Cerebral hemodynamic impairment, transpial collateralization, angiogenesis, mural cell investment, microvascular permeability, and cortical infarction after ipsilateral stroke were assessed by real-time laser speckle blood flow imaging, 2- and 3-dimensional immunofluorescence and MRI.
RESULTS—VEGF-expressing myoblasts improved hemodynamic rescue by day 14 (no EMS 37±21%, EV 42±9%, VEGF 48±12%; P<0.05 for VEGF versus no EMS and versus EV), together with the EMS take rate (VEGF 60%, EV 18.2%; P<0.05) and angiogenesis of mature cortical microvessels below the EMS (P<0.05 for VEGF versus EV). Importantly, functional and morphological results were paralleled by a 25% reduction of cortical infarction after experimental stroke on the side of the EMS.
CONCLUSIONS—Myoblast-mediated VEGF supplementation at the target site of an EMS could help overcome the clinical dilemma of poor surgical revascularization results and provide protection from ischemic stroke.</description><identifier>ISSN: 0039-2499</identifier><identifier>EISSN: 1524-4628</identifier><identifier>DOI: 10.1161/STROKEAHA.114.006712</identifier><identifier>PMID: 25388423</identifier><language>eng</language><publisher>United States: American Heart Association, Inc</publisher><subject>Animals ; Brain Ischemia ; Cerebral Revascularization ; Cerebrovascular Circulation - genetics ; Cerebrovascular Disorders ; Chronic Disease ; Collateral Circulation - genetics ; Genetic Therapy - methods ; Genetic Vectors ; Mice ; Mice, Inbred C57BL ; Myoblasts ; Neovascularization, Physiologic - genetics ; Vascular Endothelial Growth Factor A - genetics</subject><ispartof>Stroke (1970), 2015-01, Vol.46 (1), p.203-211</ispartof><rights>2015 American Heart Association, Inc.</rights><rights>2014 American Heart Association, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4682-2ceff72545b2d9aea07118359d7fb97a9b6ff22ab4c524bd4bc6a326ece10cd3</citedby><cites>FETCH-LOGICAL-c4682-2ceff72545b2d9aea07118359d7fb97a9b6ff22ab4c524bd4bc6a326ece10cd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3687,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25388423$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hecht, Nils</creatorcontrib><creatorcontrib>Marushima, Aiki</creatorcontrib><creatorcontrib>Nieminen, Melina</creatorcontrib><creatorcontrib>Kremenetskaia, Irina</creatorcontrib><creatorcontrib>von Degenfeld, Georges</creatorcontrib><creatorcontrib>Woitzik, Johannes</creatorcontrib><creatorcontrib>Vajkoczy, Peter</creatorcontrib><title>Myoblast-Mediated Gene Therapy Improves Functional Collateralization in Chronic Cerebral Hypoperfusion</title><title>Stroke (1970)</title><addtitle>Stroke</addtitle><description>BACKGROUND AND PURPOSE—Direct extracranial–intracranial bypass surgery for treatment of cerebral hemodynamic compromise remains hindered by complications but alternative simple and safe indirect revascularization procedures, such as an encephalomyosynangiosis (EMS), lack hemodynamic efficiency. Here, the myoblast-mediated transfer of angiogenic genes presents an approach for induction of therapeutic collateralization. In this study, we tested the effect of myoblast-mediated delivery of vascular endothelial growth factor-A (VEGF) to the muscle/brain interface of an EMS in a model of chronic cerebral hypoperfusion.
METHODS—Permanent unilateral internal carotid artery-occlusion was performed in adult C57/BL6 mice with or without (no EMS) surgical grafting of an EMS followed by implantation of monoclonal mouse myoblasts expressing either VEGF164 or an empty vector (EV). Cerebral hemodynamic impairment, transpial collateralization, angiogenesis, mural cell investment, microvascular permeability, and cortical infarction after ipsilateral stroke were assessed by real-time laser speckle blood flow imaging, 2- and 3-dimensional immunofluorescence and MRI.
RESULTS—VEGF-expressing myoblasts improved hemodynamic rescue by day 14 (no EMS 37±21%, EV 42±9%, VEGF 48±12%; P<0.05 for VEGF versus no EMS and versus EV), together with the EMS take rate (VEGF 60%, EV 18.2%; P<0.05) and angiogenesis of mature cortical microvessels below the EMS (P<0.05 for VEGF versus EV). Importantly, functional and morphological results were paralleled by a 25% reduction of cortical infarction after experimental stroke on the side of the EMS.
CONCLUSIONS—Myoblast-mediated VEGF supplementation at the target site of an EMS could help overcome the clinical dilemma of poor surgical revascularization results and provide protection from ischemic stroke.</description><subject>Animals</subject><subject>Brain Ischemia</subject><subject>Cerebral Revascularization</subject><subject>Cerebrovascular Circulation - genetics</subject><subject>Cerebrovascular Disorders</subject><subject>Chronic Disease</subject><subject>Collateral Circulation - genetics</subject><subject>Genetic Therapy - methods</subject><subject>Genetic Vectors</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Myoblasts</subject><subject>Neovascularization, Physiologic - genetics</subject><subject>Vascular Endothelial Growth Factor A - genetics</subject><issn>0039-2499</issn><issn>1524-4628</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMlOwzAQQC0EomX5A4R85JJiO852rKLSVhQhQe-R7YzVgBsHO6EqX49RoUdOoxm_WfwQuqFkQmlK71_XL8-Ps-liGlI-ISTNKDtBY5owHvGU5adoTEhcRIwXxQhdeP9GCGFxnpyjEUviPOcsHiP9tLfSCN9HT1A3oocaz6EFvN6AE90eL7eds5_g8cPQqr6xrTC4tMYE0gnTfImfGm5aXG6cbRuFS3AgwxNe7DvbgdODD8QVOtPCeLj-jZdo_TBbl4to9TxfltNVpHias4gp0DpjCU8kqwsBgmSU5nFS1JmWRSYKmWrNmJBchW_KmkuVipiloIASVceX6O4wNhz9MYDvq23jFYRzW7CDr2jKScxyWvCA8gOqnPXega4612yF21eUVD-Cq6PgkPLqIDi03f5uGOQW6mPTn9EA5AdgZ01w5N_NsANXbUCYfvP_7G_yi4ug</recordid><startdate>201501</startdate><enddate>201501</enddate><creator>Hecht, Nils</creator><creator>Marushima, Aiki</creator><creator>Nieminen, Melina</creator><creator>Kremenetskaia, Irina</creator><creator>von Degenfeld, Georges</creator><creator>Woitzik, Johannes</creator><creator>Vajkoczy, Peter</creator><general>American Heart Association, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201501</creationdate><title>Myoblast-Mediated Gene Therapy Improves Functional Collateralization in Chronic Cerebral Hypoperfusion</title><author>Hecht, Nils ; Marushima, Aiki ; Nieminen, Melina ; Kremenetskaia, Irina ; von Degenfeld, Georges ; Woitzik, Johannes ; Vajkoczy, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4682-2ceff72545b2d9aea07118359d7fb97a9b6ff22ab4c524bd4bc6a326ece10cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Brain Ischemia</topic><topic>Cerebral Revascularization</topic><topic>Cerebrovascular Circulation - genetics</topic><topic>Cerebrovascular Disorders</topic><topic>Chronic Disease</topic><topic>Collateral Circulation - genetics</topic><topic>Genetic Therapy - methods</topic><topic>Genetic Vectors</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Myoblasts</topic><topic>Neovascularization, Physiologic - genetics</topic><topic>Vascular Endothelial Growth Factor A - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hecht, Nils</creatorcontrib><creatorcontrib>Marushima, Aiki</creatorcontrib><creatorcontrib>Nieminen, Melina</creatorcontrib><creatorcontrib>Kremenetskaia, Irina</creatorcontrib><creatorcontrib>von Degenfeld, Georges</creatorcontrib><creatorcontrib>Woitzik, Johannes</creatorcontrib><creatorcontrib>Vajkoczy, Peter</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Stroke (1970)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hecht, Nils</au><au>Marushima, Aiki</au><au>Nieminen, Melina</au><au>Kremenetskaia, Irina</au><au>von Degenfeld, Georges</au><au>Woitzik, Johannes</au><au>Vajkoczy, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Myoblast-Mediated Gene Therapy Improves Functional Collateralization in Chronic Cerebral Hypoperfusion</atitle><jtitle>Stroke (1970)</jtitle><addtitle>Stroke</addtitle><date>2015-01</date><risdate>2015</risdate><volume>46</volume><issue>1</issue><spage>203</spage><epage>211</epage><pages>203-211</pages><issn>0039-2499</issn><eissn>1524-4628</eissn><abstract>BACKGROUND AND PURPOSE—Direct extracranial–intracranial bypass surgery for treatment of cerebral hemodynamic compromise remains hindered by complications but alternative simple and safe indirect revascularization procedures, such as an encephalomyosynangiosis (EMS), lack hemodynamic efficiency. Here, the myoblast-mediated transfer of angiogenic genes presents an approach for induction of therapeutic collateralization. In this study, we tested the effect of myoblast-mediated delivery of vascular endothelial growth factor-A (VEGF) to the muscle/brain interface of an EMS in a model of chronic cerebral hypoperfusion.
METHODS—Permanent unilateral internal carotid artery-occlusion was performed in adult C57/BL6 mice with or without (no EMS) surgical grafting of an EMS followed by implantation of monoclonal mouse myoblasts expressing either VEGF164 or an empty vector (EV). Cerebral hemodynamic impairment, transpial collateralization, angiogenesis, mural cell investment, microvascular permeability, and cortical infarction after ipsilateral stroke were assessed by real-time laser speckle blood flow imaging, 2- and 3-dimensional immunofluorescence and MRI.
RESULTS—VEGF-expressing myoblasts improved hemodynamic rescue by day 14 (no EMS 37±21%, EV 42±9%, VEGF 48±12%; P<0.05 for VEGF versus no EMS and versus EV), together with the EMS take rate (VEGF 60%, EV 18.2%; P<0.05) and angiogenesis of mature cortical microvessels below the EMS (P<0.05 for VEGF versus EV). Importantly, functional and morphological results were paralleled by a 25% reduction of cortical infarction after experimental stroke on the side of the EMS.
CONCLUSIONS—Myoblast-mediated VEGF supplementation at the target site of an EMS could help overcome the clinical dilemma of poor surgical revascularization results and provide protection from ischemic stroke.</abstract><cop>United States</cop><pub>American Heart Association, Inc</pub><pmid>25388423</pmid><doi>10.1161/STROKEAHA.114.006712</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Brain Ischemia Cerebral Revascularization Cerebrovascular Circulation - genetics Cerebrovascular Disorders Chronic Disease Collateral Circulation - genetics Genetic Therapy - methods Genetic Vectors Mice Mice, Inbred C57BL Myoblasts Neovascularization, Physiologic - genetics Vascular Endothelial Growth Factor A - genetics |
| title | Myoblast-Mediated Gene Therapy Improves Functional Collateralization in Chronic Cerebral Hypoperfusion |
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