Shenfu Injection (参附注射液) suppresses inflammation by targeting haptoglobin and pentraxin 3 in rats with chronic ischemic heart failure
Objective To investigate the regulatory effects of Shenfu Injection (SFI, 参附注射液) on hemodynamic parameters and serum proteins in rats with post-infarction chronic heart failure (CHF). Methods Forty-five healthy Wistar rats were randomized into three groups: sham, heart failure (model) and SFI group....
Gespeichert in:
| Veröffentlicht in: | Chinese journal of integrative medicine 2015, Vol.21 (1), p.22-28 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 28 |
|---|---|
| container_issue | 1 |
| container_start_page | 22 |
| container_title | Chinese journal of integrative medicine |
| container_volume | 21 |
| creator | Zheng, Si-dao Wu, Hong-jin Yu, Shao-ping Ren, Jian-xun Duo, Wei-wei Ma, Zeng-chun Gao, Yue Wang, Sheng-qi Liu, Yu-na |
| description | Objective
To investigate the regulatory effects of Shenfu Injection (SFI, 参附注射液) on hemodynamic parameters and serum proteins in rats with post-infarction chronic heart failure (CHF).
Methods
Forty-five healthy Wistar rats were randomized into three groups: sham, heart failure (model) and SFI group. The CHF was induced by left coronary artery ligation. Seven days after the surgical operation, animals in the sham group and the model group received saline (6.2 mL/kg/d), while animals in the SFI group received SFI (6.2 mL/kg d) intraperitoneally. Four weeks later, cardiac hemodynamic parameters were measured via the carotid route. The expression of serum proteins was analyzed by two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS).
Results
Recording of hemodynamic parameters showed that left ventricular systolic pressure (LVSP), maximum rate of left ventricular pressure (+dp/dt
max
) rise, and maximum rate of left ventricular pressure (−dp/dt
max
) decrease, while the left ventricular end diastolic pressure (LVEDP) rose in the model group compared to those in the sham group (
P |
| doi_str_mv | 10.1007/s11655-013-1440-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1640327246</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1640327246</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2598-57238d381052974adf6280ef387f468585396eecca2354b4f3a64f47495f85e3</originalsourceid><addsrcrecordid>eNp9kb1uFDEUha0IRH7gAdIgl6GY4P_xlFEEJFIkCtJb3tnrHa9mPIPtUUgbpU_NG1BGQeKNEilvgcMGSiof637nyL4HoX1KDikh9ftEqZKyIpRXVAhS6S20Q5uGV0QQ9qJoVbOiqdxGuymtCZG1IvIV2mZcNIIzuYNuvnQQ3IxPwxra7MeAD-5vrh6_Xz_8_HF_e_3w6-4dTvM0RUgJEvbB9XYY7B9ycYmzjSvIPqxwZ6c8rvpx4QO2YYknCDnab-XGiwtHmxO-8LnDbRfH4FvsU9vBUEQHNmbsrO_nCK_RS2f7BG-ezz10_vHD-fFJdfb50-nx0VnVMtnoStaM6yXXlEjW1MIunWKagOO6dkJpqSVvFEDbWsalWAjHrRJO1KKRTkvge-hgEzvF8esMKZuhvAf63gYY52SoEoSzmglVULpB2zimFMGZKfrBxktDiXmqwWxqMKUG81SD0cXz9jl-Xgyw_Of4u_cCsA2QyiisIJr1OMdQfvyf1N8GPJkN</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1640327246</pqid></control><display><type>article</type><title>Shenfu Injection (参附注射液) suppresses inflammation by targeting haptoglobin and pentraxin 3 in rats with chronic ischemic heart failure</title><source>MEDLINE</source><source>SpringerLink Journals</source><source>Alma/SFX Local Collection</source><creator>Zheng, Si-dao ; Wu, Hong-jin ; Yu, Shao-ping ; Ren, Jian-xun ; Duo, Wei-wei ; Ma, Zeng-chun ; Gao, Yue ; Wang, Sheng-qi ; Liu, Yu-na</creator><creatorcontrib>Zheng, Si-dao ; Wu, Hong-jin ; Yu, Shao-ping ; Ren, Jian-xun ; Duo, Wei-wei ; Ma, Zeng-chun ; Gao, Yue ; Wang, Sheng-qi ; Liu, Yu-na</creatorcontrib><description>Objective
To investigate the regulatory effects of Shenfu Injection (SFI, 参附注射液) on hemodynamic parameters and serum proteins in rats with post-infarction chronic heart failure (CHF).
Methods
Forty-five healthy Wistar rats were randomized into three groups: sham, heart failure (model) and SFI group. The CHF was induced by left coronary artery ligation. Seven days after the surgical operation, animals in the sham group and the model group received saline (6.2 mL/kg/d), while animals in the SFI group received SFI (6.2 mL/kg d) intraperitoneally. Four weeks later, cardiac hemodynamic parameters were measured via the carotid route. The expression of serum proteins was analyzed by two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS).
Results
Recording of hemodynamic parameters showed that left ventricular systolic pressure (LVSP), maximum rate of left ventricular pressure (+dp/dt
max
) rise, and maximum rate of left ventricular pressure (−dp/dt
max
) decrease, while the left ventricular end diastolic pressure (LVEDP) rose in the model group compared to those in the sham group (
P
<0.05). The results of the MALDI-TOF MS indicated that haptoglobin (HP), pentraxin 3 (PTX3) and alpha-1-antitrypsin were up-regulated, while serum albumin and 40S ribosomal protein were down-regulated in the model group (
P
<0.05). Compared with the model group, LVSP, +dp/dt
max
and −dp/dt
max
were higher, while LVEDP was lower in the SFI group (
P
<0.05). Expression levels of HP and PTX3 were lower than in the model group (
P
<0.05).
Conclusion
SFI could improve hemodynamic function and decrease inflammatory reactions in the pathophysiology of CHF. The serum proteins HP and PTX3 could be potential biomarkers for chronic ischemic heart failure, and they could also be the serum protein targets of SFI.</description><identifier>ISSN: 1672-0415</identifier><identifier>EISSN: 1993-0402</identifier><identifier>DOI: 10.1007/s11655-013-1440-8</identifier><identifier>PMID: 23494325</identifier><language>eng</language><publisher>Heidelberg: Chinese Association of Traditional and Western Medicine</publisher><subject>Animals ; Blood Proteins - metabolism ; C-Reactive Protein - metabolism ; Chronic Disease ; Drugs, Chinese Herbal - therapeutic use ; Electrophoresis, Gel, Two-Dimensional ; Haptoglobins - metabolism ; Heart Failure - blood ; Heart Failure - complications ; Heart Failure - drug therapy ; Heart Failure - physiopathology ; Heart Function Tests ; Hemodynamics ; Hydrogen-Ion Concentration ; Imaging, Three-Dimensional ; Inflammation - complications ; Inflammation - drug therapy ; Male ; Medicine ; Medicine & Public Health ; Myocardial Ischemia - blood ; Myocardial Ischemia - complications ; Myocardial Ischemia - drug therapy ; Myocardial Ischemia - physiopathology ; Original Article ; Phytotherapy ; Proteome - metabolism ; Rats, Wistar ; Serum Amyloid P-Component - metabolism ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><ispartof>Chinese journal of integrative medicine, 2015, Vol.21 (1), p.22-28</ispartof><rights>Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2598-57238d381052974adf6280ef387f468585396eecca2354b4f3a64f47495f85e3</citedby><cites>FETCH-LOGICAL-c2598-57238d381052974adf6280ef387f468585396eecca2354b4f3a64f47495f85e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11655-013-1440-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11655-013-1440-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23494325$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zheng, Si-dao</creatorcontrib><creatorcontrib>Wu, Hong-jin</creatorcontrib><creatorcontrib>Yu, Shao-ping</creatorcontrib><creatorcontrib>Ren, Jian-xun</creatorcontrib><creatorcontrib>Duo, Wei-wei</creatorcontrib><creatorcontrib>Ma, Zeng-chun</creatorcontrib><creatorcontrib>Gao, Yue</creatorcontrib><creatorcontrib>Wang, Sheng-qi</creatorcontrib><creatorcontrib>Liu, Yu-na</creatorcontrib><title>Shenfu Injection (参附注射液) suppresses inflammation by targeting haptoglobin and pentraxin 3 in rats with chronic ischemic heart failure</title><title>Chinese journal of integrative medicine</title><addtitle>Chin. J. Integr. Med</addtitle><addtitle>Chin J Integr Med</addtitle><description>Objective
To investigate the regulatory effects of Shenfu Injection (SFI, 参附注射液) on hemodynamic parameters and serum proteins in rats with post-infarction chronic heart failure (CHF).
Methods
Forty-five healthy Wistar rats were randomized into three groups: sham, heart failure (model) and SFI group. The CHF was induced by left coronary artery ligation. Seven days after the surgical operation, animals in the sham group and the model group received saline (6.2 mL/kg/d), while animals in the SFI group received SFI (6.2 mL/kg d) intraperitoneally. Four weeks later, cardiac hemodynamic parameters were measured via the carotid route. The expression of serum proteins was analyzed by two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS).
Results
Recording of hemodynamic parameters showed that left ventricular systolic pressure (LVSP), maximum rate of left ventricular pressure (+dp/dt
max
) rise, and maximum rate of left ventricular pressure (−dp/dt
max
) decrease, while the left ventricular end diastolic pressure (LVEDP) rose in the model group compared to those in the sham group (
P
<0.05). The results of the MALDI-TOF MS indicated that haptoglobin (HP), pentraxin 3 (PTX3) and alpha-1-antitrypsin were up-regulated, while serum albumin and 40S ribosomal protein were down-regulated in the model group (
P
<0.05). Compared with the model group, LVSP, +dp/dt
max
and −dp/dt
max
were higher, while LVEDP was lower in the SFI group (
P
<0.05). Expression levels of HP and PTX3 were lower than in the model group (
P
<0.05).
Conclusion
SFI could improve hemodynamic function and decrease inflammatory reactions in the pathophysiology of CHF. The serum proteins HP and PTX3 could be potential biomarkers for chronic ischemic heart failure, and they could also be the serum protein targets of SFI.</description><subject>Animals</subject><subject>Blood Proteins - metabolism</subject><subject>C-Reactive Protein - metabolism</subject><subject>Chronic Disease</subject><subject>Drugs, Chinese Herbal - therapeutic use</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Haptoglobins - metabolism</subject><subject>Heart Failure - blood</subject><subject>Heart Failure - complications</subject><subject>Heart Failure - drug therapy</subject><subject>Heart Failure - physiopathology</subject><subject>Heart Function Tests</subject><subject>Hemodynamics</subject><subject>Hydrogen-Ion Concentration</subject><subject>Imaging, Three-Dimensional</subject><subject>Inflammation - complications</subject><subject>Inflammation - drug therapy</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Myocardial Ischemia - blood</subject><subject>Myocardial Ischemia - complications</subject><subject>Myocardial Ischemia - drug therapy</subject><subject>Myocardial Ischemia - physiopathology</subject><subject>Original Article</subject><subject>Phytotherapy</subject><subject>Proteome - metabolism</subject><subject>Rats, Wistar</subject><subject>Serum Amyloid P-Component - metabolism</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><issn>1672-0415</issn><issn>1993-0402</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kb1uFDEUha0IRH7gAdIgl6GY4P_xlFEEJFIkCtJb3tnrHa9mPIPtUUgbpU_NG1BGQeKNEilvgcMGSiof637nyL4HoX1KDikh9ftEqZKyIpRXVAhS6S20Q5uGV0QQ9qJoVbOiqdxGuymtCZG1IvIV2mZcNIIzuYNuvnQQ3IxPwxra7MeAD-5vrh6_Xz_8_HF_e_3w6-4dTvM0RUgJEvbB9XYY7B9ycYmzjSvIPqxwZ6c8rvpx4QO2YYknCDnab-XGiwtHmxO-8LnDbRfH4FvsU9vBUEQHNmbsrO_nCK_RS2f7BG-ezz10_vHD-fFJdfb50-nx0VnVMtnoStaM6yXXlEjW1MIunWKagOO6dkJpqSVvFEDbWsalWAjHrRJO1KKRTkvge-hgEzvF8esMKZuhvAf63gYY52SoEoSzmglVULpB2zimFMGZKfrBxktDiXmqwWxqMKUG81SD0cXz9jl-Xgyw_Of4u_cCsA2QyiisIJr1OMdQfvyf1N8GPJkN</recordid><startdate>2015</startdate><enddate>2015</enddate><creator>Zheng, Si-dao</creator><creator>Wu, Hong-jin</creator><creator>Yu, Shao-ping</creator><creator>Ren, Jian-xun</creator><creator>Duo, Wei-wei</creator><creator>Ma, Zeng-chun</creator><creator>Gao, Yue</creator><creator>Wang, Sheng-qi</creator><creator>Liu, Yu-na</creator><general>Chinese Association of Traditional and Western Medicine</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2015</creationdate><title>Shenfu Injection (参附注射液) suppresses inflammation by targeting haptoglobin and pentraxin 3 in rats with chronic ischemic heart failure</title><author>Zheng, Si-dao ; Wu, Hong-jin ; Yu, Shao-ping ; Ren, Jian-xun ; Duo, Wei-wei ; Ma, Zeng-chun ; Gao, Yue ; Wang, Sheng-qi ; Liu, Yu-na</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2598-57238d381052974adf6280ef387f468585396eecca2354b4f3a64f47495f85e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Blood Proteins - metabolism</topic><topic>C-Reactive Protein - metabolism</topic><topic>Chronic Disease</topic><topic>Drugs, Chinese Herbal - therapeutic use</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Haptoglobins - metabolism</topic><topic>Heart Failure - blood</topic><topic>Heart Failure - complications</topic><topic>Heart Failure - drug therapy</topic><topic>Heart Failure - physiopathology</topic><topic>Heart Function Tests</topic><topic>Hemodynamics</topic><topic>Hydrogen-Ion Concentration</topic><topic>Imaging, Three-Dimensional</topic><topic>Inflammation - complications</topic><topic>Inflammation - drug therapy</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Myocardial Ischemia - blood</topic><topic>Myocardial Ischemia - complications</topic><topic>Myocardial Ischemia - drug therapy</topic><topic>Myocardial Ischemia - physiopathology</topic><topic>Original Article</topic><topic>Phytotherapy</topic><topic>Proteome - metabolism</topic><topic>Rats, Wistar</topic><topic>Serum Amyloid P-Component - metabolism</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zheng, Si-dao</creatorcontrib><creatorcontrib>Wu, Hong-jin</creatorcontrib><creatorcontrib>Yu, Shao-ping</creatorcontrib><creatorcontrib>Ren, Jian-xun</creatorcontrib><creatorcontrib>Duo, Wei-wei</creatorcontrib><creatorcontrib>Ma, Zeng-chun</creatorcontrib><creatorcontrib>Gao, Yue</creatorcontrib><creatorcontrib>Wang, Sheng-qi</creatorcontrib><creatorcontrib>Liu, Yu-na</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Chinese journal of integrative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zheng, Si-dao</au><au>Wu, Hong-jin</au><au>Yu, Shao-ping</au><au>Ren, Jian-xun</au><au>Duo, Wei-wei</au><au>Ma, Zeng-chun</au><au>Gao, Yue</au><au>Wang, Sheng-qi</au><au>Liu, Yu-na</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Shenfu Injection (参附注射液) suppresses inflammation by targeting haptoglobin and pentraxin 3 in rats with chronic ischemic heart failure</atitle><jtitle>Chinese journal of integrative medicine</jtitle><stitle>Chin. J. Integr. Med</stitle><addtitle>Chin J Integr Med</addtitle><date>2015</date><risdate>2015</risdate><volume>21</volume><issue>1</issue><spage>22</spage><epage>28</epage><pages>22-28</pages><issn>1672-0415</issn><eissn>1993-0402</eissn><abstract>Objective
To investigate the regulatory effects of Shenfu Injection (SFI, 参附注射液) on hemodynamic parameters and serum proteins in rats with post-infarction chronic heart failure (CHF).
Methods
Forty-five healthy Wistar rats were randomized into three groups: sham, heart failure (model) and SFI group. The CHF was induced by left coronary artery ligation. Seven days after the surgical operation, animals in the sham group and the model group received saline (6.2 mL/kg/d), while animals in the SFI group received SFI (6.2 mL/kg d) intraperitoneally. Four weeks later, cardiac hemodynamic parameters were measured via the carotid route. The expression of serum proteins was analyzed by two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS).
Results
Recording of hemodynamic parameters showed that left ventricular systolic pressure (LVSP), maximum rate of left ventricular pressure (+dp/dt
max
) rise, and maximum rate of left ventricular pressure (−dp/dt
max
) decrease, while the left ventricular end diastolic pressure (LVEDP) rose in the model group compared to those in the sham group (
P
<0.05). The results of the MALDI-TOF MS indicated that haptoglobin (HP), pentraxin 3 (PTX3) and alpha-1-antitrypsin were up-regulated, while serum albumin and 40S ribosomal protein were down-regulated in the model group (
P
<0.05). Compared with the model group, LVSP, +dp/dt
max
and −dp/dt
max
were higher, while LVEDP was lower in the SFI group (
P
<0.05). Expression levels of HP and PTX3 were lower than in the model group (
P
<0.05).
Conclusion
SFI could improve hemodynamic function and decrease inflammatory reactions in the pathophysiology of CHF. The serum proteins HP and PTX3 could be potential biomarkers for chronic ischemic heart failure, and they could also be the serum protein targets of SFI.</abstract><cop>Heidelberg</cop><pub>Chinese Association of Traditional and Western Medicine</pub><pmid>23494325</pmid><doi>10.1007/s11655-013-1440-8</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1672-0415 |
| ispartof | Chinese journal of integrative medicine, 2015, Vol.21 (1), p.22-28 |
| issn | 1672-0415 1993-0402 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1640327246 |
| source | MEDLINE; SpringerLink Journals; Alma/SFX Local Collection |
| subjects | Animals Blood Proteins - metabolism C-Reactive Protein - metabolism Chronic Disease Drugs, Chinese Herbal - therapeutic use Electrophoresis, Gel, Two-Dimensional Haptoglobins - metabolism Heart Failure - blood Heart Failure - complications Heart Failure - drug therapy Heart Failure - physiopathology Heart Function Tests Hemodynamics Hydrogen-Ion Concentration Imaging, Three-Dimensional Inflammation - complications Inflammation - drug therapy Male Medicine Medicine & Public Health Myocardial Ischemia - blood Myocardial Ischemia - complications Myocardial Ischemia - drug therapy Myocardial Ischemia - physiopathology Original Article Phytotherapy Proteome - metabolism Rats, Wistar Serum Amyloid P-Component - metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| title | Shenfu Injection (参附注射液) suppresses inflammation by targeting haptoglobin and pentraxin 3 in rats with chronic ischemic heart failure |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T09%3A38%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Shenfu%20Injection%20(%E5%8F%82%E9%99%84%E6%B3%A8%E5%B0%84%E6%B6%B2)%20suppresses%20inflammation%20by%20targeting%20haptoglobin%20and%20pentraxin%203%20in%20rats%20with%20chronic%20ischemic%20heart%20failure&rft.jtitle=Chinese%20journal%20of%20integrative%20medicine&rft.au=Zheng,%20Si-dao&rft.date=2015&rft.volume=21&rft.issue=1&rft.spage=22&rft.epage=28&rft.pages=22-28&rft.issn=1672-0415&rft.eissn=1993-0402&rft_id=info:doi/10.1007/s11655-013-1440-8&rft_dat=%3Cproquest_cross%3E1640327246%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1640327246&rft_id=info:pmid/23494325&rfr_iscdi=true |