Evidence for the presence of N-acetylaspartylglutamate in cultured oligodendrocytes and LPS activated microglia
The levels of N-acetylaspartylglutamate (NAAG) were determined by HPLC in untreated or lipopolysaccharide (LPS) activated pure astrocyte, oligodendrocyte, and microglial cultures derived from developing rat brain. Oligodendrocyte cultures expressed 1.52±0.12 nmol/ μg protein of NAAG, whereas astrocy...
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| Veröffentlicht in: | Brain research 1998-05, Vol.794 (1), p.143-145 |
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| creator | Passani, L Elkabes, S Coyle, J.T |
| description | The levels of
N-acetylaspartylglutamate (NAAG) were determined by HPLC in untreated or lipopolysaccharide (LPS) activated pure astrocyte, oligodendrocyte, and microglial cultures derived from developing rat brain. Oligodendrocyte cultures expressed 1.52±0.12 nmol/
μg protein of NAAG, whereas astrocyte cultures (0.04±0.08 nmol/
μg protein) and untreated microglial cultures (0.05±0.09 nmol/
μg protein) contained only trace amounts of the dipeptide. After stimulation of microglial cultures for 24 h with LPS, NAAG levels increased significantly to 0.37±0.12 SD nmol/
μg protein. NAAG levels in astrocyte and oligodendrocyte cultures remained unchanged after LPS treatment. The findings indicate that NAAG is localized to specific glial cell types. Further our results suggest that NAAG biosynthesis is induced in microglia, activated by specific stimuli. |
| doi_str_mv | 10.1016/S0006-8993(98)00308-4 |
| format | Article |
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N-acetylaspartylglutamate (NAAG) were determined by HPLC in untreated or lipopolysaccharide (LPS) activated pure astrocyte, oligodendrocyte, and microglial cultures derived from developing rat brain. Oligodendrocyte cultures expressed 1.52±0.12 nmol/
μg protein of NAAG, whereas astrocyte cultures (0.04±0.08 nmol/
μg protein) and untreated microglial cultures (0.05±0.09 nmol/
μg protein) contained only trace amounts of the dipeptide. After stimulation of microglial cultures for 24 h with LPS, NAAG levels increased significantly to 0.37±0.12 SD nmol/
μg protein. NAAG levels in astrocyte and oligodendrocyte cultures remained unchanged after LPS treatment. The findings indicate that NAAG is localized to specific glial cell types. Further our results suggest that NAAG biosynthesis is induced in microglia, activated by specific stimuli.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/S0006-8993(98)00308-4</identifier><identifier>PMID: 9630582</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Alzheimer's disease ; Animals ; Astrocytes - chemistry ; Astrocytes - drug effects ; Biological and medical sciences ; Cells, Cultured ; Chromatography, High Pressure Liquid ; Dipeptides - analysis ; Excitatory amino acid ; Fundamental and applied biological sciences. Psychology ; Huntington's disease ; Isolated neuron and nerve. Neuroglia ; Lipopolysaccharides - pharmacology ; Male ; Microglia ; Microglia - chemistry ; Microglia - drug effects ; N-acetylaspartylglutamate ; Neuropeptides - analysis ; Oligodendrocyte ; Oligodendroglia - chemistry ; Peptide ; Rats ; Rats, Sprague-Dawley ; Vertebrates: nervous system and sense organs</subject><ispartof>Brain research, 1998-05, Vol.794 (1), p.143-145</ispartof><rights>1998 Elsevier Science B.V.</rights><rights>1998 INIST-CNRS</rights><rights>Copyright 1998 Elsevier Science B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-264bd29f09924f041256f2355311556bcc94083fb3151f342b07662300e553be3</citedby><cites>FETCH-LOGICAL-c420t-264bd29f09924f041256f2355311556bcc94083fb3151f342b07662300e553be3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0006-8993(98)00308-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2311429$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9630582$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Passani, L</creatorcontrib><creatorcontrib>Elkabes, S</creatorcontrib><creatorcontrib>Coyle, J.T</creatorcontrib><title>Evidence for the presence of N-acetylaspartylglutamate in cultured oligodendrocytes and LPS activated microglia</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>The levels of
N-acetylaspartylglutamate (NAAG) were determined by HPLC in untreated or lipopolysaccharide (LPS) activated pure astrocyte, oligodendrocyte, and microglial cultures derived from developing rat brain. Oligodendrocyte cultures expressed 1.52±0.12 nmol/
μg protein of NAAG, whereas astrocyte cultures (0.04±0.08 nmol/
μg protein) and untreated microglial cultures (0.05±0.09 nmol/
μg protein) contained only trace amounts of the dipeptide. After stimulation of microglial cultures for 24 h with LPS, NAAG levels increased significantly to 0.37±0.12 SD nmol/
μg protein. NAAG levels in astrocyte and oligodendrocyte cultures remained unchanged after LPS treatment. The findings indicate that NAAG is localized to specific glial cell types. Further our results suggest that NAAG biosynthesis is induced in microglia, activated by specific stimuli.</description><subject>Alzheimer's disease</subject><subject>Animals</subject><subject>Astrocytes - chemistry</subject><subject>Astrocytes - drug effects</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Dipeptides - analysis</subject><subject>Excitatory amino acid</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Huntington's disease</subject><subject>Isolated neuron and nerve. Neuroglia</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Male</subject><subject>Microglia</subject><subject>Microglia - chemistry</subject><subject>Microglia - drug effects</subject><subject>N-acetylaspartylglutamate</subject><subject>Neuropeptides - analysis</subject><subject>Oligodendrocyte</subject><subject>Oligodendroglia - chemistry</subject><subject>Peptide</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEuLFDEQgIMo67j6ExZyENFDa-XR2c5JlmV9wKDC6jmk05Ux0t0Zk_TA_HszD-bqqaiqryqVj5AbBu8ZMPXhEQBU02kt3uruHYCArpFPyIp1t7xRXMJTsrogz8mLnP_UVAgNV-RKKwFtx1ckPuzCgLND6mOi5TfSbcJ8LERPvzXWYdmPNm9tqnEzLsVOtiANM3XLWJaEA41j2MS6ZEjR7QtmaueBrn88UutK2FV6oFNwKW7GYF-SZ96OGV-d4zX59enh5_2XZv3989f7u3XjJIfScCX7gWsPWnPpQTLeKs9F2wrG2lb1zmkJnfC9YC3zQvIebpXiAgAr06O4Jm9Oe7cp_l0wFzOF7HAc7YxxyYYpCbxjXQXbE1gPzDmhN9sUJpv2hoE5iDZH0eZg0ejOHEUbWeduzg8s_YTDZepstvZfn_s2Ozv6ZGcX8gXj9SOS64p9PGFYZewCJpNdOOgfQkJXzBDDfw75B049mfg</recordid><startdate>19980525</startdate><enddate>19980525</enddate><creator>Passani, L</creator><creator>Elkabes, S</creator><creator>Coyle, J.T</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>19980525</creationdate><title>Evidence for the presence of N-acetylaspartylglutamate in cultured oligodendrocytes and LPS activated microglia</title><author>Passani, L ; Elkabes, S ; Coyle, J.T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-264bd29f09924f041256f2355311556bcc94083fb3151f342b07662300e553be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Alzheimer's disease</topic><topic>Animals</topic><topic>Astrocytes - chemistry</topic><topic>Astrocytes - drug effects</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Dipeptides - analysis</topic><topic>Excitatory amino acid</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Huntington's disease</topic><topic>Isolated neuron and nerve. Neuroglia</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Male</topic><topic>Microglia</topic><topic>Microglia - chemistry</topic><topic>Microglia - drug effects</topic><topic>N-acetylaspartylglutamate</topic><topic>Neuropeptides - analysis</topic><topic>Oligodendrocyte</topic><topic>Oligodendroglia - chemistry</topic><topic>Peptide</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Passani, L</creatorcontrib><creatorcontrib>Elkabes, S</creatorcontrib><creatorcontrib>Coyle, J.T</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Passani, L</au><au>Elkabes, S</au><au>Coyle, J.T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence for the presence of N-acetylaspartylglutamate in cultured oligodendrocytes and LPS activated microglia</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1998-05-25</date><risdate>1998</risdate><volume>794</volume><issue>1</issue><spage>143</spage><epage>145</epage><pages>143-145</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>The levels of
N-acetylaspartylglutamate (NAAG) were determined by HPLC in untreated or lipopolysaccharide (LPS) activated pure astrocyte, oligodendrocyte, and microglial cultures derived from developing rat brain. Oligodendrocyte cultures expressed 1.52±0.12 nmol/
μg protein of NAAG, whereas astrocyte cultures (0.04±0.08 nmol/
μg protein) and untreated microglial cultures (0.05±0.09 nmol/
μg protein) contained only trace amounts of the dipeptide. After stimulation of microglial cultures for 24 h with LPS, NAAG levels increased significantly to 0.37±0.12 SD nmol/
μg protein. NAAG levels in astrocyte and oligodendrocyte cultures remained unchanged after LPS treatment. The findings indicate that NAAG is localized to specific glial cell types. Further our results suggest that NAAG biosynthesis is induced in microglia, activated by specific stimuli.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>9630582</pmid><doi>10.1016/S0006-8993(98)00308-4</doi><tpages>3</tpages></addata></record> |
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| subjects | Alzheimer's disease Animals Astrocytes - chemistry Astrocytes - drug effects Biological and medical sciences Cells, Cultured Chromatography, High Pressure Liquid Dipeptides - analysis Excitatory amino acid Fundamental and applied biological sciences. Psychology Huntington's disease Isolated neuron and nerve. Neuroglia Lipopolysaccharides - pharmacology Male Microglia Microglia - chemistry Microglia - drug effects N-acetylaspartylglutamate Neuropeptides - analysis Oligodendrocyte Oligodendroglia - chemistry Peptide Rats Rats, Sprague-Dawley Vertebrates: nervous system and sense organs |
| title | Evidence for the presence of N-acetylaspartylglutamate in cultured oligodendrocytes and LPS activated microglia |
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