Multiparameter analyses of three-dimensionally cultured tumor spheroids based on respiratory activity and comprehensive gene expression profiles

Multicellular spheroids of human breast cancer cells (MCF-7) formed with two different three-dimensional (3D) culture methods were evaluated in detail on the basis of respiratory activity and high-throughput gene expression analysis. The spheroids formed with poly(dimethylsiloxane) (PDMS) microwell...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Analytical biochemistry 2013-08, Vol.439 (2), p.187-193
Hauptverfasser:	Zhou, Yuanshu, Arai, Toshiharu, Horiguchi, Yoshiko, Ino, Kosuke, Matsue, Tomokazu, Shiku, Hitoshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Breast Neoplasms - metabolism Cell Culture Techniques - methods Cell Line, Tumor Energy Metabolism Female Gene Expression Regulation, Neoplastic - physiology High-throughput qPCR Humans Multicellular tumor spheroids Oxygen Consumption - physiology PDMS microwell array Protein Array Analysis - methods Respiratory activity Transcriptome - physiology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!