Multiparameter analyses of three-dimensionally cultured tumor spheroids based on respiratory activity and comprehensive gene expression profiles
Multicellular spheroids of human breast cancer cells (MCF-7) formed with two different three-dimensional (3D) culture methods were evaluated in detail on the basis of respiratory activity and high-throughput gene expression analysis. The spheroids formed with poly(dimethylsiloxane) (PDMS) microwell...
Gespeichert in:
| Veröffentlicht in: | Analytical biochemistry 2013-08, Vol.439 (2), p.187-193 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 193 |
|---|---|
| container_issue | 2 |
| container_start_page | 187 |
| container_title | Analytical biochemistry |
| container_volume | 439 |
| creator | Zhou, Yuanshu Arai, Toshiharu Horiguchi, Yoshiko Ino, Kosuke Matsue, Tomokazu Shiku, Hitoshi |
| description | Multicellular spheroids of human breast cancer cells (MCF-7) formed with two different three-dimensional (3D) culture methods were evaluated in detail on the basis of respiratory activity and high-throughput gene expression analysis. The spheroids formed with poly(dimethylsiloxane) (PDMS) microwell arrays indicated significant restriction of the spheroid size, whereas their respiratory activity was 2-fold greater than that formed with the hanging drop culture method. Fluidigm BioMark dynamic array was used for comprehensive and quantitative real-time polymerase chain reaction (qRT–PCR) analysis on the samples whose respiratory activity had been measured. Genes involved in cellular senescence and glucose metabolism indicated significantly higher values for the PDMS microwell culture method than for the hanging drop culture method (P |
| doi_str_mv | 10.1016/j.ab.2013.04.020 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1639992794</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0003269713001942</els_id><sourcerecordid>1400400812</sourcerecordid><originalsourceid>FETCH-LOGICAL-c383t-a8febb50b984a448f572a5853f858c2d0d8b2f4a12069e3270eb2d59f61fce2f3</originalsourceid><addsrcrecordid>eNqFkcGOFCEQhonRuLOrd0-Go5ceC-juAW9ms-oma7zomdBQOEy6mxboifMWPrJMZvVmTEiKVH31kfAT8orBlgHr3x62ZthyYGIL7RY4PCEbBqpvQIB6SjYAIBreq90Vuc75AMBY2_XPyRUXPZeCsw359XkdS1hMMhMWTNTMZjxlzDR6WvYJsXFhwjmHWAfjidqKrwkdLesUE83LHlMMLtPB5NqNM02Yl5BMielEjS3hGEq9zI7aOC0J92fZEel3nJHiz9rJZzldUvRhxPyCPPNmzPjysd6Qbx_uvt5-ah6-fLy_ff_QWCFFaYz0OAwdDEq2pm2l73bcdLITXnbScgdODty3hnHoFQq-Axy465TvmbfIvbghby7e-vCPFXPRU8gWx9HMGNesWS-UUnyn2v-jLUA9kvGKwgW1Keac0Oslhcmkk2agz5HpgzaDPkemodU1srry-tG-DhO6vwt_MqrAuwuA9TuOAZPONuBs0YWEtmgXw7_tvwFJYqoW</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1400400812</pqid></control><display><type>article</type><title>Multiparameter analyses of three-dimensionally cultured tumor spheroids based on respiratory activity and comprehensive gene expression profiles</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Zhou, Yuanshu ; Arai, Toshiharu ; Horiguchi, Yoshiko ; Ino, Kosuke ; Matsue, Tomokazu ; Shiku, Hitoshi</creator><creatorcontrib>Zhou, Yuanshu ; Arai, Toshiharu ; Horiguchi, Yoshiko ; Ino, Kosuke ; Matsue, Tomokazu ; Shiku, Hitoshi</creatorcontrib><description>Multicellular spheroids of human breast cancer cells (MCF-7) formed with two different three-dimensional (3D) culture methods were evaluated in detail on the basis of respiratory activity and high-throughput gene expression analysis. The spheroids formed with poly(dimethylsiloxane) (PDMS) microwell arrays indicated significant restriction of the spheroid size, whereas their respiratory activity was 2-fold greater than that formed with the hanging drop culture method. Fluidigm BioMark dynamic array was used for comprehensive and quantitative real-time polymerase chain reaction (qRT–PCR) analysis on the samples whose respiratory activity had been measured. Genes involved in cellular senescence and glucose metabolism indicated significantly higher values for the PDMS microwell culture method than for the hanging drop culture method (P<0.05). Interestingly, samples formed with the PDMS microwell culture method showed stronger responses for glycolysis than those formed with the hanging drop method. These results illustrate the power of multiparameter analysis to characterize multicellular spheroids cultured in different microenvironments even if they have the same morphology.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/j.ab.2013.04.020</identifier><identifier>PMID: 23628321</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Breast Neoplasms - metabolism ; Cell Culture Techniques - methods ; Cell Line, Tumor ; Energy Metabolism ; Female ; Gene Expression Regulation, Neoplastic - physiology ; High-throughput qPCR ; Humans ; Multicellular tumor spheroids ; Oxygen Consumption - physiology ; PDMS microwell array ; Protein Array Analysis - methods ; Respiratory activity ; Transcriptome - physiology</subject><ispartof>Analytical biochemistry, 2013-08, Vol.439 (2), p.187-193</ispartof><rights>2013 Elsevier Inc.</rights><rights>Copyright © 2013 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-a8febb50b984a448f572a5853f858c2d0d8b2f4a12069e3270eb2d59f61fce2f3</citedby><cites>FETCH-LOGICAL-c383t-a8febb50b984a448f572a5853f858c2d0d8b2f4a12069e3270eb2d59f61fce2f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ab.2013.04.020$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3541,27915,27916,45986</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23628321$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Yuanshu</creatorcontrib><creatorcontrib>Arai, Toshiharu</creatorcontrib><creatorcontrib>Horiguchi, Yoshiko</creatorcontrib><creatorcontrib>Ino, Kosuke</creatorcontrib><creatorcontrib>Matsue, Tomokazu</creatorcontrib><creatorcontrib>Shiku, Hitoshi</creatorcontrib><title>Multiparameter analyses of three-dimensionally cultured tumor spheroids based on respiratory activity and comprehensive gene expression profiles</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>Multicellular spheroids of human breast cancer cells (MCF-7) formed with two different three-dimensional (3D) culture methods were evaluated in detail on the basis of respiratory activity and high-throughput gene expression analysis. The spheroids formed with poly(dimethylsiloxane) (PDMS) microwell arrays indicated significant restriction of the spheroid size, whereas their respiratory activity was 2-fold greater than that formed with the hanging drop culture method. Fluidigm BioMark dynamic array was used for comprehensive and quantitative real-time polymerase chain reaction (qRT–PCR) analysis on the samples whose respiratory activity had been measured. Genes involved in cellular senescence and glucose metabolism indicated significantly higher values for the PDMS microwell culture method than for the hanging drop culture method (P<0.05). Interestingly, samples formed with the PDMS microwell culture method showed stronger responses for glycolysis than those formed with the hanging drop method. These results illustrate the power of multiparameter analysis to characterize multicellular spheroids cultured in different microenvironments even if they have the same morphology.</description><subject>Breast Neoplasms - metabolism</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Line, Tumor</subject><subject>Energy Metabolism</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic - physiology</subject><subject>High-throughput qPCR</subject><subject>Humans</subject><subject>Multicellular tumor spheroids</subject><subject>Oxygen Consumption - physiology</subject><subject>PDMS microwell array</subject><subject>Protein Array Analysis - methods</subject><subject>Respiratory activity</subject><subject>Transcriptome - physiology</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcGOFCEQhonRuLOrd0-Go5ceC-juAW9ms-oma7zomdBQOEy6mxboifMWPrJMZvVmTEiKVH31kfAT8orBlgHr3x62ZthyYGIL7RY4PCEbBqpvQIB6SjYAIBreq90Vuc75AMBY2_XPyRUXPZeCsw359XkdS1hMMhMWTNTMZjxlzDR6WvYJsXFhwjmHWAfjidqKrwkdLesUE83LHlMMLtPB5NqNM02Yl5BMielEjS3hGEq9zI7aOC0J92fZEel3nJHiz9rJZzldUvRhxPyCPPNmzPjysd6Qbx_uvt5-ah6-fLy_ff_QWCFFaYz0OAwdDEq2pm2l73bcdLITXnbScgdODty3hnHoFQq-Axy465TvmbfIvbghby7e-vCPFXPRU8gWx9HMGNesWS-UUnyn2v-jLUA9kvGKwgW1Keac0Oslhcmkk2agz5HpgzaDPkemodU1srry-tG-DhO6vwt_MqrAuwuA9TuOAZPONuBs0YWEtmgXw7_tvwFJYqoW</recordid><startdate>20130815</startdate><enddate>20130815</enddate><creator>Zhou, Yuanshu</creator><creator>Arai, Toshiharu</creator><creator>Horiguchi, Yoshiko</creator><creator>Ino, Kosuke</creator><creator>Matsue, Tomokazu</creator><creator>Shiku, Hitoshi</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20130815</creationdate><title>Multiparameter analyses of three-dimensionally cultured tumor spheroids based on respiratory activity and comprehensive gene expression profiles</title><author>Zhou, Yuanshu ; Arai, Toshiharu ; Horiguchi, Yoshiko ; Ino, Kosuke ; Matsue, Tomokazu ; Shiku, Hitoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-a8febb50b984a448f572a5853f858c2d0d8b2f4a12069e3270eb2d59f61fce2f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Breast Neoplasms - metabolism</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Line, Tumor</topic><topic>Energy Metabolism</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic - physiology</topic><topic>High-throughput qPCR</topic><topic>Humans</topic><topic>Multicellular tumor spheroids</topic><topic>Oxygen Consumption - physiology</topic><topic>PDMS microwell array</topic><topic>Protein Array Analysis - methods</topic><topic>Respiratory activity</topic><topic>Transcriptome - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Yuanshu</creatorcontrib><creatorcontrib>Arai, Toshiharu</creatorcontrib><creatorcontrib>Horiguchi, Yoshiko</creatorcontrib><creatorcontrib>Ino, Kosuke</creatorcontrib><creatorcontrib>Matsue, Tomokazu</creatorcontrib><creatorcontrib>Shiku, Hitoshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Yuanshu</au><au>Arai, Toshiharu</au><au>Horiguchi, Yoshiko</au><au>Ino, Kosuke</au><au>Matsue, Tomokazu</au><au>Shiku, Hitoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multiparameter analyses of three-dimensionally cultured tumor spheroids based on respiratory activity and comprehensive gene expression profiles</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>2013-08-15</date><risdate>2013</risdate><volume>439</volume><issue>2</issue><spage>187</spage><epage>193</epage><pages>187-193</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>Multicellular spheroids of human breast cancer cells (MCF-7) formed with two different three-dimensional (3D) culture methods were evaluated in detail on the basis of respiratory activity and high-throughput gene expression analysis. The spheroids formed with poly(dimethylsiloxane) (PDMS) microwell arrays indicated significant restriction of the spheroid size, whereas their respiratory activity was 2-fold greater than that formed with the hanging drop culture method. Fluidigm BioMark dynamic array was used for comprehensive and quantitative real-time polymerase chain reaction (qRT–PCR) analysis on the samples whose respiratory activity had been measured. Genes involved in cellular senescence and glucose metabolism indicated significantly higher values for the PDMS microwell culture method than for the hanging drop culture method (P<0.05). Interestingly, samples formed with the PDMS microwell culture method showed stronger responses for glycolysis than those formed with the hanging drop method. These results illustrate the power of multiparameter analysis to characterize multicellular spheroids cultured in different microenvironments even if they have the same morphology.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>23628321</pmid><doi>10.1016/j.ab.2013.04.020</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 2013-08, Vol.439 (2), p.187-193 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1639992794 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Breast Neoplasms - metabolism Cell Culture Techniques - methods Cell Line, Tumor Energy Metabolism Female Gene Expression Regulation, Neoplastic - physiology High-throughput qPCR Humans Multicellular tumor spheroids Oxygen Consumption - physiology PDMS microwell array Protein Array Analysis - methods Respiratory activity Transcriptome - physiology |
| title | Multiparameter analyses of three-dimensionally cultured tumor spheroids based on respiratory activity and comprehensive gene expression profiles |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T19%3A58%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Multiparameter%20analyses%20of%20three-dimensionally%20cultured%20tumor%20spheroids%20based%20on%20respiratory%20activity%20and%20comprehensive%20gene%20expression%20profiles&rft.jtitle=Analytical%20biochemistry&rft.au=Zhou,%20Yuanshu&rft.date=2013-08-15&rft.volume=439&rft.issue=2&rft.spage=187&rft.epage=193&rft.pages=187-193&rft.issn=0003-2697&rft.eissn=1096-0309&rft_id=info:doi/10.1016/j.ab.2013.04.020&rft_dat=%3Cproquest_cross%3E1400400812%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1400400812&rft_id=info:pmid/23628321&rft_els_id=S0003269713001942&rfr_iscdi=true |