Morphological and proteomic characterization of midgut of the malaria vector Anopheles albimanus at early time after a blood feeding
The midgut of anopheline mosquito is the entry of Plasmodium, the causative agent of malaria.When the mosquito feeds on parasite infected host, Plasmodium parasites reach the midgut and must confront digestive enzymes, the innate immune response and go across the peritrophic matrix (PM), a thick ext...
Gespeichert in:
Veröffentlicht in: | Journal of proteomics 2014-12, Vol.111, p.100-112 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 112 |
---|---|
container_issue | |
container_start_page | 100 |
container_title | Journal of proteomics |
container_volume | 111 |
creator | Cázares-Raga, F.E. Chávez-Munguía, B. González-Calixto, C. Ochoa-Franco, A.P. Gawinowicz, M.A. Rodríguez, M.H. Hernández-Hernández, F.C. |
description | The midgut of anopheline mosquito is the entry of Plasmodium, the causative agent of malaria.When the mosquito feeds on parasite infected host, Plasmodium parasites reach the midgut and must confront digestive enzymes, the innate immune response and go across the peritrophic matrix (PM), a thick extracellular sheath secreted by the mosquito midgut epithelial cells. Then, to continue its development, the parasite must reach the salivary glands to achieve transmission to a vertebrate host.
We report here the morphological and biochemical descriptions of the midgut changes after a blood meal in Anopheles albimanus. Before blood feeding, midgut epithelial cells contained numerous electrondense vesicles distributed in the central to apical side. These vesicles were secreted to the luminal side of the midgut after a blood meal. At early times after blood ingest, the PM is formed near microvilli as a granulous amorphous material and after it consolidates forming a highly organized fibrillar structure, constituted by layers of electrondense and electronlucent regions. Proteomic comparative analysis of sugar and blood fed midguts showed several molecules that modify their abundance after blood intake; these include innate immunity, cytoskeletal, stress response, signaling, and digestive, detoxifying and metabolism enzymes.
Biological significance
In the midgut of mosquitoes during bloodfeeding, many simultaneous processes occur, including digestion, innate immune activities, cytoskeleton modifications, construction of a peritrophic matrix and hormone production, between others. Mechanical forces are very intense during bloodfeeding and epithelial and muscular cells must resist the stress, modifying the actin cytoskeleton and coordinating intracellular responses by signaling. Microorganisms present in midgut contents reproduce and interact with epithelial cells triggering innate immune response. When infectious agents are present in the blood meal they must traverse the peritrophic matrix, an envelope formed from secretion products of epithelial cells, and evade the immune system in order to reach the epithelium and continue their journey towards salivary glands, in preparation for the transmission to the new hosts. During all these processes, proteins of mosquitoes are modified in order to deal with mechanical and biological challenges, and the aim of this work is to study these changes. This article is part of a Special Issue entitled: Proteomics, mass spectrometr |
doi_str_mv | 10.1016/j.jprot.2014.07.037 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1639991780</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1874391914003984</els_id><sourcerecordid>1639991780</sourcerecordid><originalsourceid>FETCH-LOGICAL-c392t-e65986082014c22631276a79318cf7156671d011fa696d86b926103850cfec9f3</originalsourceid><addsrcrecordid>eNp9kD9vHCEQxVGUKHZsf4JIEWWa3TDLHSyFC8ty_kiO0iQ14tjhjhO7nIG15NT54GZ9tstU84o3b-b9CPkIrAUG4su-3R9SLG3HYNUy2TIu35BT6KVopOTy7ZNeNVyBOiEfct4zJkAq-Z6cdGvgHazglPz7GdNhF0PcemsCNdNAl1CMo7fU7kwytmDyf03xcaLR0dEP27ksquyQjiaY5A29R1tioldTPOwwYKYmbPxoprmqQtGk8ECLH5EaV-OooZsQ40Ad4uCn7Tl550zIePE8z8ifrze_r783t7--_bi-um0sV11pUKxVL1i_FLZdJzh0UhipOPTWSVgLIWFgAM4IJYZebFQngPF-zaxDqxw_I5-PubXi3Yy56NFniyGYCeOcNQiulALZs2rlR6tNMeeETh9SLZQeNDC94Nd7_YRfL99oJnXFX7c-PR-YNyMOrzsvvKvh8mjAWvPeY9LZepxsxZAqQj1E_98Dj6R_mGo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1639991780</pqid></control><display><type>article</type><title>Morphological and proteomic characterization of midgut of the malaria vector Anopheles albimanus at early time after a blood feeding</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Cázares-Raga, F.E. ; Chávez-Munguía, B. ; González-Calixto, C. ; Ochoa-Franco, A.P. ; Gawinowicz, M.A. ; Rodríguez, M.H. ; Hernández-Hernández, F.C.</creator><creatorcontrib>Cázares-Raga, F.E. ; Chávez-Munguía, B. ; González-Calixto, C. ; Ochoa-Franco, A.P. ; Gawinowicz, M.A. ; Rodríguez, M.H. ; Hernández-Hernández, F.C.</creatorcontrib><description>The midgut of anopheline mosquito is the entry of Plasmodium, the causative agent of malaria.When the mosquito feeds on parasite infected host, Plasmodium parasites reach the midgut and must confront digestive enzymes, the innate immune response and go across the peritrophic matrix (PM), a thick extracellular sheath secreted by the mosquito midgut epithelial cells. Then, to continue its development, the parasite must reach the salivary glands to achieve transmission to a vertebrate host.
We report here the morphological and biochemical descriptions of the midgut changes after a blood meal in Anopheles albimanus. Before blood feeding, midgut epithelial cells contained numerous electrondense vesicles distributed in the central to apical side. These vesicles were secreted to the luminal side of the midgut after a blood meal. At early times after blood ingest, the PM is formed near microvilli as a granulous amorphous material and after it consolidates forming a highly organized fibrillar structure, constituted by layers of electrondense and electronlucent regions. Proteomic comparative analysis of sugar and blood fed midguts showed several molecules that modify their abundance after blood intake; these include innate immunity, cytoskeletal, stress response, signaling, and digestive, detoxifying and metabolism enzymes.
Biological significance
In the midgut of mosquitoes during bloodfeeding, many simultaneous processes occur, including digestion, innate immune activities, cytoskeleton modifications, construction of a peritrophic matrix and hormone production, between others. Mechanical forces are very intense during bloodfeeding and epithelial and muscular cells must resist the stress, modifying the actin cytoskeleton and coordinating intracellular responses by signaling. Microorganisms present in midgut contents reproduce and interact with epithelial cells triggering innate immune response. When infectious agents are present in the blood meal they must traverse the peritrophic matrix, an envelope formed from secretion products of epithelial cells, and evade the immune system in order to reach the epithelium and continue their journey towards salivary glands, in preparation for the transmission to the new hosts. During all these processes, proteins of mosquitoes are modified in order to deal with mechanical and biological challenges, and the aim of this work is to study these changes. This article is part of a Special Issue entitled: Proteomics, mass spectrometry and peptidomics, Cancun 2013. Guest Editors: César López-Camarillo, Victoria Pando-Robles and Bronwyn Jane Barkla.
[Display omitted]
•Mosquito midgut is formed by a monolayer epithelium supported by muscular strands.•Dense granules, present in resting midgut cells are emptied after blood feeding.•Dense granules contents are resynthesized 12 to 24h after blood feeding.•Blood feeding modifies midgut cytoskeleton proteins during mechanical stress.•Blood feeding modifies stress and immune proteins present in the midgut.</description><identifier>ISSN: 1874-3919</identifier><identifier>EISSN: 1876-7737</identifier><identifier>DOI: 10.1016/j.jprot.2014.07.037</identifier><identifier>PMID: 25132141</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Anopheles - metabolism ; Anopheles - parasitology ; Anopheles albimanus ; Anopheles mosquito ; Bloodfeeding ; Cytoskeleton - metabolism ; Digestive System - metabolism ; Electron microscopy ; Electrophoresis, Gel, Two-Dimensional ; Epithelial Cells - parasitology ; Female ; Host-Parasite Interactions ; Humans ; Immunity, Innate ; Insect Vectors - metabolism ; Insect Vectors - parasitology ; Mice ; Mice, Inbred BALB C ; Midgut ; Oxidative Stress ; Perithrophic matrix ; Plasmodium ; Plasmodium - metabolism ; Proteome ; Proteomics ; Serpins - chemistry ; Signal Transduction ; Spectrometry, Mass, Electrospray Ionization ; Tandem Mass Spectrometry ; Time Factors</subject><ispartof>Journal of proteomics, 2014-12, Vol.111, p.100-112</ispartof><rights>2014 Elsevier B.V.</rights><rights>Copyright © 2014 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-e65986082014c22631276a79318cf7156671d011fa696d86b926103850cfec9f3</citedby><cites>FETCH-LOGICAL-c392t-e65986082014c22631276a79318cf7156671d011fa696d86b926103850cfec9f3</cites><orcidid>0000-0002-7276-7513</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jprot.2014.07.037$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25132141$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cázares-Raga, F.E.</creatorcontrib><creatorcontrib>Chávez-Munguía, B.</creatorcontrib><creatorcontrib>González-Calixto, C.</creatorcontrib><creatorcontrib>Ochoa-Franco, A.P.</creatorcontrib><creatorcontrib>Gawinowicz, M.A.</creatorcontrib><creatorcontrib>Rodríguez, M.H.</creatorcontrib><creatorcontrib>Hernández-Hernández, F.C.</creatorcontrib><title>Morphological and proteomic characterization of midgut of the malaria vector Anopheles albimanus at early time after a blood feeding</title><title>Journal of proteomics</title><addtitle>J Proteomics</addtitle><description>The midgut of anopheline mosquito is the entry of Plasmodium, the causative agent of malaria.When the mosquito feeds on parasite infected host, Plasmodium parasites reach the midgut and must confront digestive enzymes, the innate immune response and go across the peritrophic matrix (PM), a thick extracellular sheath secreted by the mosquito midgut epithelial cells. Then, to continue its development, the parasite must reach the salivary glands to achieve transmission to a vertebrate host.
We report here the morphological and biochemical descriptions of the midgut changes after a blood meal in Anopheles albimanus. Before blood feeding, midgut epithelial cells contained numerous electrondense vesicles distributed in the central to apical side. These vesicles were secreted to the luminal side of the midgut after a blood meal. At early times after blood ingest, the PM is formed near microvilli as a granulous amorphous material and after it consolidates forming a highly organized fibrillar structure, constituted by layers of electrondense and electronlucent regions. Proteomic comparative analysis of sugar and blood fed midguts showed several molecules that modify their abundance after blood intake; these include innate immunity, cytoskeletal, stress response, signaling, and digestive, detoxifying and metabolism enzymes.
Biological significance
In the midgut of mosquitoes during bloodfeeding, many simultaneous processes occur, including digestion, innate immune activities, cytoskeleton modifications, construction of a peritrophic matrix and hormone production, between others. Mechanical forces are very intense during bloodfeeding and epithelial and muscular cells must resist the stress, modifying the actin cytoskeleton and coordinating intracellular responses by signaling. Microorganisms present in midgut contents reproduce and interact with epithelial cells triggering innate immune response. When infectious agents are present in the blood meal they must traverse the peritrophic matrix, an envelope formed from secretion products of epithelial cells, and evade the immune system in order to reach the epithelium and continue their journey towards salivary glands, in preparation for the transmission to the new hosts. During all these processes, proteins of mosquitoes are modified in order to deal with mechanical and biological challenges, and the aim of this work is to study these changes. This article is part of a Special Issue entitled: Proteomics, mass spectrometry and peptidomics, Cancun 2013. Guest Editors: César López-Camarillo, Victoria Pando-Robles and Bronwyn Jane Barkla.
[Display omitted]
•Mosquito midgut is formed by a monolayer epithelium supported by muscular strands.•Dense granules, present in resting midgut cells are emptied after blood feeding.•Dense granules contents are resynthesized 12 to 24h after blood feeding.•Blood feeding modifies midgut cytoskeleton proteins during mechanical stress.•Blood feeding modifies stress and immune proteins present in the midgut.</description><subject>Animals</subject><subject>Anopheles - metabolism</subject><subject>Anopheles - parasitology</subject><subject>Anopheles albimanus</subject><subject>Anopheles mosquito</subject><subject>Bloodfeeding</subject><subject>Cytoskeleton - metabolism</subject><subject>Digestive System - metabolism</subject><subject>Electron microscopy</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Epithelial Cells - parasitology</subject><subject>Female</subject><subject>Host-Parasite Interactions</subject><subject>Humans</subject><subject>Immunity, Innate</subject><subject>Insect Vectors - metabolism</subject><subject>Insect Vectors - parasitology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Midgut</subject><subject>Oxidative Stress</subject><subject>Perithrophic matrix</subject><subject>Plasmodium</subject><subject>Plasmodium - metabolism</subject><subject>Proteome</subject><subject>Proteomics</subject><subject>Serpins - chemistry</subject><subject>Signal Transduction</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>Tandem Mass Spectrometry</subject><subject>Time Factors</subject><issn>1874-3919</issn><issn>1876-7737</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD9vHCEQxVGUKHZsf4JIEWWa3TDLHSyFC8ty_kiO0iQ14tjhjhO7nIG15NT54GZ9tstU84o3b-b9CPkIrAUG4su-3R9SLG3HYNUy2TIu35BT6KVopOTy7ZNeNVyBOiEfct4zJkAq-Z6cdGvgHazglPz7GdNhF0PcemsCNdNAl1CMo7fU7kwytmDyf03xcaLR0dEP27ksquyQjiaY5A29R1tioldTPOwwYKYmbPxoprmqQtGk8ECLH5EaV-OooZsQ40Ad4uCn7Tl550zIePE8z8ifrze_r783t7--_bi-um0sV11pUKxVL1i_FLZdJzh0UhipOPTWSVgLIWFgAM4IJYZebFQngPF-zaxDqxw_I5-PubXi3Yy56NFniyGYCeOcNQiulALZs2rlR6tNMeeETh9SLZQeNDC94Nd7_YRfL99oJnXFX7c-PR-YNyMOrzsvvKvh8mjAWvPeY9LZepxsxZAqQj1E_98Dj6R_mGo</recordid><startdate>20141205</startdate><enddate>20141205</enddate><creator>Cázares-Raga, F.E.</creator><creator>Chávez-Munguía, B.</creator><creator>González-Calixto, C.</creator><creator>Ochoa-Franco, A.P.</creator><creator>Gawinowicz, M.A.</creator><creator>Rodríguez, M.H.</creator><creator>Hernández-Hernández, F.C.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>M7N</scope><orcidid>https://orcid.org/0000-0002-7276-7513</orcidid></search><sort><creationdate>20141205</creationdate><title>Morphological and proteomic characterization of midgut of the malaria vector Anopheles albimanus at early time after a blood feeding</title><author>Cázares-Raga, F.E. ; Chávez-Munguía, B. ; González-Calixto, C. ; Ochoa-Franco, A.P. ; Gawinowicz, M.A. ; Rodríguez, M.H. ; Hernández-Hernández, F.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-e65986082014c22631276a79318cf7156671d011fa696d86b926103850cfec9f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Anopheles - metabolism</topic><topic>Anopheles - parasitology</topic><topic>Anopheles albimanus</topic><topic>Anopheles mosquito</topic><topic>Bloodfeeding</topic><topic>Cytoskeleton - metabolism</topic><topic>Digestive System - metabolism</topic><topic>Electron microscopy</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Epithelial Cells - parasitology</topic><topic>Female</topic><topic>Host-Parasite Interactions</topic><topic>Humans</topic><topic>Immunity, Innate</topic><topic>Insect Vectors - metabolism</topic><topic>Insect Vectors - parasitology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Midgut</topic><topic>Oxidative Stress</topic><topic>Perithrophic matrix</topic><topic>Plasmodium</topic><topic>Plasmodium - metabolism</topic><topic>Proteome</topic><topic>Proteomics</topic><topic>Serpins - chemistry</topic><topic>Signal Transduction</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><topic>Tandem Mass Spectrometry</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cázares-Raga, F.E.</creatorcontrib><creatorcontrib>Chávez-Munguía, B.</creatorcontrib><creatorcontrib>González-Calixto, C.</creatorcontrib><creatorcontrib>Ochoa-Franco, A.P.</creatorcontrib><creatorcontrib>Gawinowicz, M.A.</creatorcontrib><creatorcontrib>Rodríguez, M.H.</creatorcontrib><creatorcontrib>Hernández-Hernández, F.C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Journal of proteomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cázares-Raga, F.E.</au><au>Chávez-Munguía, B.</au><au>González-Calixto, C.</au><au>Ochoa-Franco, A.P.</au><au>Gawinowicz, M.A.</au><au>Rodríguez, M.H.</au><au>Hernández-Hernández, F.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Morphological and proteomic characterization of midgut of the malaria vector Anopheles albimanus at early time after a blood feeding</atitle><jtitle>Journal of proteomics</jtitle><addtitle>J Proteomics</addtitle><date>2014-12-05</date><risdate>2014</risdate><volume>111</volume><spage>100</spage><epage>112</epage><pages>100-112</pages><issn>1874-3919</issn><eissn>1876-7737</eissn><abstract>The midgut of anopheline mosquito is the entry of Plasmodium, the causative agent of malaria.When the mosquito feeds on parasite infected host, Plasmodium parasites reach the midgut and must confront digestive enzymes, the innate immune response and go across the peritrophic matrix (PM), a thick extracellular sheath secreted by the mosquito midgut epithelial cells. Then, to continue its development, the parasite must reach the salivary glands to achieve transmission to a vertebrate host.
We report here the morphological and biochemical descriptions of the midgut changes after a blood meal in Anopheles albimanus. Before blood feeding, midgut epithelial cells contained numerous electrondense vesicles distributed in the central to apical side. These vesicles were secreted to the luminal side of the midgut after a blood meal. At early times after blood ingest, the PM is formed near microvilli as a granulous amorphous material and after it consolidates forming a highly organized fibrillar structure, constituted by layers of electrondense and electronlucent regions. Proteomic comparative analysis of sugar and blood fed midguts showed several molecules that modify their abundance after blood intake; these include innate immunity, cytoskeletal, stress response, signaling, and digestive, detoxifying and metabolism enzymes.
Biological significance
In the midgut of mosquitoes during bloodfeeding, many simultaneous processes occur, including digestion, innate immune activities, cytoskeleton modifications, construction of a peritrophic matrix and hormone production, between others. Mechanical forces are very intense during bloodfeeding and epithelial and muscular cells must resist the stress, modifying the actin cytoskeleton and coordinating intracellular responses by signaling. Microorganisms present in midgut contents reproduce and interact with epithelial cells triggering innate immune response. When infectious agents are present in the blood meal they must traverse the peritrophic matrix, an envelope formed from secretion products of epithelial cells, and evade the immune system in order to reach the epithelium and continue their journey towards salivary glands, in preparation for the transmission to the new hosts. During all these processes, proteins of mosquitoes are modified in order to deal with mechanical and biological challenges, and the aim of this work is to study these changes. This article is part of a Special Issue entitled: Proteomics, mass spectrometry and peptidomics, Cancun 2013. Guest Editors: César López-Camarillo, Victoria Pando-Robles and Bronwyn Jane Barkla.
[Display omitted]
•Mosquito midgut is formed by a monolayer epithelium supported by muscular strands.•Dense granules, present in resting midgut cells are emptied after blood feeding.•Dense granules contents are resynthesized 12 to 24h after blood feeding.•Blood feeding modifies midgut cytoskeleton proteins during mechanical stress.•Blood feeding modifies stress and immune proteins present in the midgut.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>25132141</pmid><doi>10.1016/j.jprot.2014.07.037</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-7276-7513</orcidid></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1874-3919 |
ispartof | Journal of proteomics, 2014-12, Vol.111, p.100-112 |
issn | 1874-3919 1876-7737 |
language | eng |
recordid | cdi_proquest_miscellaneous_1639991780 |
source | MEDLINE; Access via ScienceDirect (Elsevier) |
subjects | Animals Anopheles - metabolism Anopheles - parasitology Anopheles albimanus Anopheles mosquito Bloodfeeding Cytoskeleton - metabolism Digestive System - metabolism Electron microscopy Electrophoresis, Gel, Two-Dimensional Epithelial Cells - parasitology Female Host-Parasite Interactions Humans Immunity, Innate Insect Vectors - metabolism Insect Vectors - parasitology Mice Mice, Inbred BALB C Midgut Oxidative Stress Perithrophic matrix Plasmodium Plasmodium - metabolism Proteome Proteomics Serpins - chemistry Signal Transduction Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry Time Factors |
title | Morphological and proteomic characterization of midgut of the malaria vector Anopheles albimanus at early time after a blood feeding |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T00%3A07%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Morphological%20and%20proteomic%20characterization%20of%20midgut%20of%20the%20malaria%20vector%20Anopheles%20albimanus%20at%20early%20time%20after%20a%20blood%20feeding&rft.jtitle=Journal%20of%20proteomics&rft.au=C%C3%A1zares-Raga,%20F.E.&rft.date=2014-12-05&rft.volume=111&rft.spage=100&rft.epage=112&rft.pages=100-112&rft.issn=1874-3919&rft.eissn=1876-7737&rft_id=info:doi/10.1016/j.jprot.2014.07.037&rft_dat=%3Cproquest_cross%3E1639991780%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1639991780&rft_id=info:pmid/25132141&rft_els_id=S1874391914003984&rfr_iscdi=true |