Analysis of genetically modified organisms by pyrosequencing on a portable photodiode-based bioluminescence sequencer
•A portable bioluminescence analyzer for detecting the DNA sequence was developed.•The DNA sequences of eight genes of GMO were successfully detected.•The corresponding limit of detection (LOD) was 0.01% with 35 PCR cycles.•The results indicate the method is suitable for the field test of GMO analys...
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| Veröffentlicht in: | Food chemistry 2014-07, Vol.154, p.78-83 |
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| creator | Song, Qinxin Wei, Guijiang Zhou, Guohua |
| description | •A portable bioluminescence analyzer for detecting the DNA sequence was developed.•The DNA sequences of eight genes of GMO were successfully detected.•The corresponding limit of detection (LOD) was 0.01% with 35 PCR cycles.•The results indicate the method is suitable for the field test of GMO analysis.
A portable bioluminescence analyser for detecting the DNA sequence of genetically modified organisms (GMOs) was developed by using a photodiode (PD) array. Pyrosequencing on eight genes (zSSIIb, Bt11 and Bt176 gene of genetically modified maize; Lectin, 35S-CTP4, CP4EPSPS, CaMV35S promoter and NOS terminator of the genetically modified Roundup ready soya) was successfully detected with this instrument. The corresponding limit of detection (LOD) was 0.01% with 35 PCR cycles. The maize and soya available from three different provenances in China were detected. The results indicate that pyrosequencing using the small size of the detector is a simple, inexpensive, and reliable way in a farm/field test of GMO analysis. |
| doi_str_mv | 10.1016/j.foodchem.2014.01.001 |
| format | Article |
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A portable bioluminescence analyser for detecting the DNA sequence of genetically modified organisms (GMOs) was developed by using a photodiode (PD) array. Pyrosequencing on eight genes (zSSIIb, Bt11 and Bt176 gene of genetically modified maize; Lectin, 35S-CTP4, CP4EPSPS, CaMV35S promoter and NOS terminator of the genetically modified Roundup ready soya) was successfully detected with this instrument. The corresponding limit of detection (LOD) was 0.01% with 35 PCR cycles. The maize and soya available from three different provenances in China were detected. The results indicate that pyrosequencing using the small size of the detector is a simple, inexpensive, and reliable way in a farm/field test of GMO analysis.</description><identifier>ISSN: 0308-8146</identifier><identifier>EISSN: 1873-7072</identifier><identifier>DOI: 10.1016/j.foodchem.2014.01.001</identifier><identifier>PMID: 24518318</identifier><identifier>CODEN: FOCHDJ</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Biological and medical sciences ; Bt-11 and Bt-176 maize ; DNA, Plant - chemistry ; DNA, Plant - genetics ; Food toxicology ; Food, Genetically Modified ; Genetically modified organisms (GMO) ; Glycine max - chemistry ; Glycine max - genetics ; High-Throughput Nucleotide Sequencing - instrumentation ; High-Throughput Nucleotide Sequencing - methods ; Luminescence ; Medical sciences ; Plants, Genetically Modified - chemistry ; Plants, Genetically Modified - genetics ; Polymerase Chain Reaction ; Portable bioluminescence analyser ; Pyrosequencing ; Roundup ready soya ; Toxicology ; Zea mays ; Zea mays - chemistry ; Zea mays - genetics</subject><ispartof>Food chemistry, 2014-07, Vol.154, p.78-83</ispartof><rights>2014 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c497t-d9ac75dff630da241c9b10ea564fabbde810726b3075b97d0df9b425e601edb03</citedby><cites>FETCH-LOGICAL-c497t-d9ac75dff630da241c9b10ea564fabbde810726b3075b97d0df9b425e601edb03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.foodchem.2014.01.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28409698$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24518318$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Song, Qinxin</creatorcontrib><creatorcontrib>Wei, Guijiang</creatorcontrib><creatorcontrib>Zhou, Guohua</creatorcontrib><title>Analysis of genetically modified organisms by pyrosequencing on a portable photodiode-based bioluminescence sequencer</title><title>Food chemistry</title><addtitle>Food Chem</addtitle><description>•A portable bioluminescence analyzer for detecting the DNA sequence was developed.•The DNA sequences of eight genes of GMO were successfully detected.•The corresponding limit of detection (LOD) was 0.01% with 35 PCR cycles.•The results indicate the method is suitable for the field test of GMO analysis.
A portable bioluminescence analyser for detecting the DNA sequence of genetically modified organisms (GMOs) was developed by using a photodiode (PD) array. Pyrosequencing on eight genes (zSSIIb, Bt11 and Bt176 gene of genetically modified maize; Lectin, 35S-CTP4, CP4EPSPS, CaMV35S promoter and NOS terminator of the genetically modified Roundup ready soya) was successfully detected with this instrument. The corresponding limit of detection (LOD) was 0.01% with 35 PCR cycles. The maize and soya available from three different provenances in China were detected. The results indicate that pyrosequencing using the small size of the detector is a simple, inexpensive, and reliable way in a farm/field test of GMO analysis.</description><subject>Biological and medical sciences</subject><subject>Bt-11 and Bt-176 maize</subject><subject>DNA, Plant - chemistry</subject><subject>DNA, Plant - genetics</subject><subject>Food toxicology</subject><subject>Food, Genetically Modified</subject><subject>Genetically modified organisms (GMO)</subject><subject>Glycine max - chemistry</subject><subject>Glycine max - genetics</subject><subject>High-Throughput Nucleotide Sequencing - instrumentation</subject><subject>High-Throughput Nucleotide Sequencing - methods</subject><subject>Luminescence</subject><subject>Medical sciences</subject><subject>Plants, Genetically Modified - chemistry</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Polymerase Chain Reaction</subject><subject>Portable bioluminescence analyser</subject><subject>Pyrosequencing</subject><subject>Roundup ready soya</subject><subject>Toxicology</subject><subject>Zea mays</subject><subject>Zea mays - chemistry</subject><subject>Zea mays - genetics</subject><issn>0308-8146</issn><issn>1873-7072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuO1DAQRS0EYpqGXxh5g8QmTTlxEnvHaMRLGokNrC0_yj1uJXGwE6T8PW51Dyxn5c05VeV7CbllcGDAuo-ng4_R2UccDzUwfgB2AGAvyI6Jvql66OuXZAcNiEow3t2QNzmfAKCw4jW5qXnLRMPEjqx3kx62HDKNnh5xwiVYPQwbHaMLPqCjMR31FPKYqdnovKWY8feKkw3TkcaJajrHtGgzIJ0f41Ks6LAyOhfVhDisY5gw2yIgvZqY3pJXXg8Z313fPfn15fPP-2_Vw4-v3-_vHirLZb9UTmrbt877rgGna86sNAxQtx332hiHgpWPdqaBvjWyd-C8NLxusQOGzkCzJx8uc-cUy-q8qDGUW4ZBTxjXrFjXSClk3YvnUS4l47Usyp50F9SWMHJCr-YURp02xUCd21En9dSOOrejgKnSThFvrztWM6L7pz3VUYD3V0DnUoNPuuSc_3OCg-zkmft04bCE9ydgUtmGc7IuJLSLcjE8d8tfjx-z3g</recordid><startdate>20140701</startdate><enddate>20140701</enddate><creator>Song, Qinxin</creator><creator>Wei, Guijiang</creator><creator>Zhou, Guohua</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20140701</creationdate><title>Analysis of genetically modified organisms by pyrosequencing on a portable photodiode-based bioluminescence sequencer</title><author>Song, Qinxin ; Wei, Guijiang ; Zhou, Guohua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c497t-d9ac75dff630da241c9b10ea564fabbde810726b3075b97d0df9b425e601edb03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Biological and medical sciences</topic><topic>Bt-11 and Bt-176 maize</topic><topic>DNA, Plant - chemistry</topic><topic>DNA, Plant - genetics</topic><topic>Food toxicology</topic><topic>Food, Genetically Modified</topic><topic>Genetically modified organisms (GMO)</topic><topic>Glycine max - chemistry</topic><topic>Glycine max - genetics</topic><topic>High-Throughput Nucleotide Sequencing - instrumentation</topic><topic>High-Throughput Nucleotide Sequencing - methods</topic><topic>Luminescence</topic><topic>Medical sciences</topic><topic>Plants, Genetically Modified - chemistry</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Polymerase Chain Reaction</topic><topic>Portable bioluminescence analyser</topic><topic>Pyrosequencing</topic><topic>Roundup ready soya</topic><topic>Toxicology</topic><topic>Zea mays</topic><topic>Zea mays - chemistry</topic><topic>Zea mays - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Song, Qinxin</creatorcontrib><creatorcontrib>Wei, Guijiang</creatorcontrib><creatorcontrib>Zhou, Guohua</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Song, Qinxin</au><au>Wei, Guijiang</au><au>Zhou, Guohua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of genetically modified organisms by pyrosequencing on a portable photodiode-based bioluminescence sequencer</atitle><jtitle>Food chemistry</jtitle><addtitle>Food Chem</addtitle><date>2014-07-01</date><risdate>2014</risdate><volume>154</volume><spage>78</spage><epage>83</epage><pages>78-83</pages><issn>0308-8146</issn><eissn>1873-7072</eissn><coden>FOCHDJ</coden><abstract>•A portable bioluminescence analyzer for detecting the DNA sequence was developed.•The DNA sequences of eight genes of GMO were successfully detected.•The corresponding limit of detection (LOD) was 0.01% with 35 PCR cycles.•The results indicate the method is suitable for the field test of GMO analysis.
A portable bioluminescence analyser for detecting the DNA sequence of genetically modified organisms (GMOs) was developed by using a photodiode (PD) array. Pyrosequencing on eight genes (zSSIIb, Bt11 and Bt176 gene of genetically modified maize; Lectin, 35S-CTP4, CP4EPSPS, CaMV35S promoter and NOS terminator of the genetically modified Roundup ready soya) was successfully detected with this instrument. The corresponding limit of detection (LOD) was 0.01% with 35 PCR cycles. The maize and soya available from three different provenances in China were detected. The results indicate that pyrosequencing using the small size of the detector is a simple, inexpensive, and reliable way in a farm/field test of GMO analysis.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>24518318</pmid><doi>10.1016/j.foodchem.2014.01.001</doi><tpages>6</tpages></addata></record> |
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| subjects | Biological and medical sciences Bt-11 and Bt-176 maize DNA, Plant - chemistry DNA, Plant - genetics Food toxicology Food, Genetically Modified Genetically modified organisms (GMO) Glycine max - chemistry Glycine max - genetics High-Throughput Nucleotide Sequencing - instrumentation High-Throughput Nucleotide Sequencing - methods Luminescence Medical sciences Plants, Genetically Modified - chemistry Plants, Genetically Modified - genetics Polymerase Chain Reaction Portable bioluminescence analyser Pyrosequencing Roundup ready soya Toxicology Zea mays Zea mays - chemistry Zea mays - genetics |
| title | Analysis of genetically modified organisms by pyrosequencing on a portable photodiode-based bioluminescence sequencer |
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