Impaired DNA Repair as Assessed by the “Comet” Assay in Patients with Thyroid Tumors After a History of Radiation Therapy: A Preliminary Study
Purpose: Patients with a history of head and neck irradiation in childhood are at risk to develop thyroid tumors. The aim of this study was to determine if an impairement of DNA strand breaks repair could account for this observation. Methods and Materials: Circulating unstimulated lymphocytes of a...
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| Veröffentlicht in: | International journal of radiation oncology, biology, physics biology, physics, 1998-03, Vol.40 (5), p.1019-1026 |
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| container_title | International journal of radiation oncology, biology, physics |
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| creator | Leprat, Frederic Alapetite, Claire Rosselli, Filippo Ridet, Agnès Schlumberger, Martin Sarasin, Alain Suarez, Horacio G. Moustacchi, Ethel |
| description | Purpose: Patients with a history of head and neck irradiation in childhood are at risk to develop thyroid tumors. The aim of this study was to determine if an impairement of DNA strand breaks repair could account for this observation.
Methods and Materials: Circulating unstimulated lymphocytes of a group of 13 patients who developed thyroid tumors after radiotherapy were submitted to the alkaline single-cell gel electrophoresis assay (SCGE or “comet” assay) after
in vitro exposure to 2 and 5 Gy of γ-rays. A control group of 8 healthy donors and 2 cases with a history of neck irradiation who did not develop a thyroid tumor were also analysed. The immediate response was compared to that observed after 15, 30, and 60 min of postexposure incubation periods.
Results: Induction of DNA strand breaks is a dose–dependent process. The SCGE assay parameters did not differ significantly between patients and controls immediately (
t = 0) after irradiation at the two doses used. As compared to healthy donors, a slower kinetics of repair was found in the patients. The proportion of residual damage at 60 min postirradiation was significantly (
p < 0.01) higher in patients than in controls, at both doses analysed. Flow cytometric analysis of apoptosis and p53 protein status studied before and after irradiation showed no apparent relationship with the repair capacity.
Conclusion: This preliminary study suggests that a subgroup of patients who develop thyroid tumors after a history of irradiation are partially defective in the late restitution of
in vitro radiation-induced DNA strand breaks. This deficiency could be a predisposing factor to radiation-associated thyroid tumorigenesis. Detection of susceptible individuals using the simple and rapid comet assay, especially children receiving radiotherapeutic treatment, may allow a preventive surveillance for radiation-associated epithelial thyroid tumor development. |
| doi_str_mv | 10.1016/S0360-3016(97)00914-0 |
| format | Article |
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Methods and Materials: Circulating unstimulated lymphocytes of a group of 13 patients who developed thyroid tumors after radiotherapy were submitted to the alkaline single-cell gel electrophoresis assay (SCGE or “comet” assay) after
in vitro exposure to 2 and 5 Gy of γ-rays. A control group of 8 healthy donors and 2 cases with a history of neck irradiation who did not develop a thyroid tumor were also analysed. The immediate response was compared to that observed after 15, 30, and 60 min of postexposure incubation periods.
Results: Induction of DNA strand breaks is a dose–dependent process. The SCGE assay parameters did not differ significantly between patients and controls immediately (
t = 0) after irradiation at the two doses used. As compared to healthy donors, a slower kinetics of repair was found in the patients. The proportion of residual damage at 60 min postirradiation was significantly (
p < 0.01) higher in patients than in controls, at both doses analysed. Flow cytometric analysis of apoptosis and p53 protein status studied before and after irradiation showed no apparent relationship with the repair capacity.
Conclusion: This preliminary study suggests that a subgroup of patients who develop thyroid tumors after a history of irradiation are partially defective in the late restitution of
in vitro radiation-induced DNA strand breaks. This deficiency could be a predisposing factor to radiation-associated thyroid tumorigenesis. Detection of susceptible individuals using the simple and rapid comet assay, especially children receiving radiotherapeutic treatment, may allow a preventive surveillance for radiation-associated epithelial thyroid tumor development.</description><identifier>ISSN: 0360-3016</identifier><identifier>EISSN: 1879-355X</identifier><identifier>DOI: 10.1016/S0360-3016(97)00914-0</identifier><identifier>PMID: 9539555</identifier><identifier>CODEN: IOBPD3</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adult ; Apoptosis ; Biological and medical sciences ; Blood leukocytes ; Carcinogenesis, carcinogens and anticarcinogens ; DNA - radiation effects ; DNA Damage ; DNA Repair ; Electrophoresis - methods ; Endocrinopathies ; Female ; Humans ; Male ; Malignant tumors ; Medical sciences ; Middle Aged ; Neoplasms, Radiation-Induced - genetics ; Physical agents ; Radiation-induced ; Single cell gel electrophoresis assay ; Thyroid cancer ; Thyroid Gland - radiation effects ; Thyroid Neoplasms - genetics ; Thyroid. Thyroid axis (diseases) ; Tumors</subject><ispartof>International journal of radiation oncology, biology, physics, 1998-03, Vol.40 (5), p.1019-1026</ispartof><rights>1998 Elsevier Science Inc.</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-b1516da8df4ca8a09c3e99465f3f11b70364061c0fb79b1cc397c41dec3203ae3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0360301697009140$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2214361$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9539555$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Leprat, Frederic</creatorcontrib><creatorcontrib>Alapetite, Claire</creatorcontrib><creatorcontrib>Rosselli, Filippo</creatorcontrib><creatorcontrib>Ridet, Agnès</creatorcontrib><creatorcontrib>Schlumberger, Martin</creatorcontrib><creatorcontrib>Sarasin, Alain</creatorcontrib><creatorcontrib>Suarez, Horacio G.</creatorcontrib><creatorcontrib>Moustacchi, Ethel</creatorcontrib><title>Impaired DNA Repair as Assessed by the “Comet” Assay in Patients with Thyroid Tumors After a History of Radiation Therapy: A Preliminary Study</title><title>International journal of radiation oncology, biology, physics</title><addtitle>Int J Radiat Oncol Biol Phys</addtitle><description>Purpose: Patients with a history of head and neck irradiation in childhood are at risk to develop thyroid tumors. The aim of this study was to determine if an impairement of DNA strand breaks repair could account for this observation.
Methods and Materials: Circulating unstimulated lymphocytes of a group of 13 patients who developed thyroid tumors after radiotherapy were submitted to the alkaline single-cell gel electrophoresis assay (SCGE or “comet” assay) after
in vitro exposure to 2 and 5 Gy of γ-rays. A control group of 8 healthy donors and 2 cases with a history of neck irradiation who did not develop a thyroid tumor were also analysed. The immediate response was compared to that observed after 15, 30, and 60 min of postexposure incubation periods.
Results: Induction of DNA strand breaks is a dose–dependent process. The SCGE assay parameters did not differ significantly between patients and controls immediately (
t = 0) after irradiation at the two doses used. As compared to healthy donors, a slower kinetics of repair was found in the patients. The proportion of residual damage at 60 min postirradiation was significantly (
p < 0.01) higher in patients than in controls, at both doses analysed. Flow cytometric analysis of apoptosis and p53 protein status studied before and after irradiation showed no apparent relationship with the repair capacity.
Conclusion: This preliminary study suggests that a subgroup of patients who develop thyroid tumors after a history of irradiation are partially defective in the late restitution of
in vitro radiation-induced DNA strand breaks. This deficiency could be a predisposing factor to radiation-associated thyroid tumorigenesis. Detection of susceptible individuals using the simple and rapid comet assay, especially children receiving radiotherapeutic treatment, may allow a preventive surveillance for radiation-associated epithelial thyroid tumor development.</description><subject>Adult</subject><subject>Apoptosis</subject><subject>Biological and medical sciences</subject><subject>Blood leukocytes</subject><subject>Carcinogenesis, carcinogens and anticarcinogens</subject><subject>DNA - radiation effects</subject><subject>DNA Damage</subject><subject>DNA Repair</subject><subject>Electrophoresis - methods</subject><subject>Endocrinopathies</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Malignant tumors</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Neoplasms, Radiation-Induced - genetics</subject><subject>Physical agents</subject><subject>Radiation-induced</subject><subject>Single cell gel electrophoresis assay</subject><subject>Thyroid cancer</subject><subject>Thyroid Gland - radiation effects</subject><subject>Thyroid Neoplasms - genetics</subject><subject>Thyroid. Thyroid axis (diseases)</subject><subject>Tumors</subject><issn>0360-3016</issn><issn>1879-355X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd9qFDEUxoModa0-QiEXInoxmkwmMxtvZFntHyha2hW8C5nkhI3MTNYko8xdn8Hr9uX6JGa7y94KgSSc3_ly8n0InVDynhJaf7ghrCYFy8e3onlHiKBVQZ6gGZ03omCc_3iKZgfkOXoR409CCKVNdYSOBGeCcz5Dfy_6jXIBDP78dYGvYXvBKuJFjJCXwe2E0xrww-3d0veQHm7vtzU1YTfgK5UcDCniPy6t8Wo9Be8MXo29D1nBJshS-NzF5MOEvcXXyrjc4ofMQlCb6SNe4KsAnevdoDJzk0YzvUTPrOoivNrvx-j76ZfV8ry4_HZ2sVxcFroqSSpaymlt1NzYSqu5IkIzEKKquWWW0rbJf69ITTWxbSNaqjUTja6oAc1KwhSwY_Rmp7sJ_tcIMcneRQ1dpwbwY5S0ZkLMS55BvgN18DEGsHITXJ_nlZTIbRbyMQu5NVqKRj5mIUnuO9k_MLY9mEPX3vxcf72vq6hVZ4MatIsHrCxpxWqasU87DLIZvx0EGXW2XYPJuekkjXf_GeQfKI2oKg</recordid><startdate>19980315</startdate><enddate>19980315</enddate><creator>Leprat, Frederic</creator><creator>Alapetite, Claire</creator><creator>Rosselli, Filippo</creator><creator>Ridet, Agnès</creator><creator>Schlumberger, Martin</creator><creator>Sarasin, Alain</creator><creator>Suarez, Horacio G.</creator><creator>Moustacchi, Ethel</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>19980315</creationdate><title>Impaired DNA Repair as Assessed by the “Comet” Assay in Patients with Thyroid Tumors After a History of Radiation Therapy: A Preliminary Study</title><author>Leprat, Frederic ; Alapetite, Claire ; Rosselli, Filippo ; Ridet, Agnès ; Schlumberger, Martin ; Sarasin, Alain ; Suarez, Horacio G. ; Moustacchi, Ethel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-b1516da8df4ca8a09c3e99465f3f11b70364061c0fb79b1cc397c41dec3203ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Adult</topic><topic>Apoptosis</topic><topic>Biological and medical sciences</topic><topic>Blood leukocytes</topic><topic>Carcinogenesis, carcinogens and anticarcinogens</topic><topic>DNA - radiation effects</topic><topic>DNA Damage</topic><topic>DNA Repair</topic><topic>Electrophoresis - methods</topic><topic>Endocrinopathies</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Malignant tumors</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Neoplasms, Radiation-Induced - genetics</topic><topic>Physical agents</topic><topic>Radiation-induced</topic><topic>Single cell gel electrophoresis assay</topic><topic>Thyroid cancer</topic><topic>Thyroid Gland - radiation effects</topic><topic>Thyroid Neoplasms - genetics</topic><topic>Thyroid. Thyroid axis (diseases)</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Leprat, Frederic</creatorcontrib><creatorcontrib>Alapetite, Claire</creatorcontrib><creatorcontrib>Rosselli, Filippo</creatorcontrib><creatorcontrib>Ridet, Agnès</creatorcontrib><creatorcontrib>Schlumberger, Martin</creatorcontrib><creatorcontrib>Sarasin, Alain</creatorcontrib><creatorcontrib>Suarez, Horacio G.</creatorcontrib><creatorcontrib>Moustacchi, Ethel</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>International journal of radiation oncology, biology, physics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leprat, Frederic</au><au>Alapetite, Claire</au><au>Rosselli, Filippo</au><au>Ridet, Agnès</au><au>Schlumberger, Martin</au><au>Sarasin, Alain</au><au>Suarez, Horacio G.</au><au>Moustacchi, Ethel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impaired DNA Repair as Assessed by the “Comet” Assay in Patients with Thyroid Tumors After a History of Radiation Therapy: A Preliminary Study</atitle><jtitle>International journal of radiation oncology, biology, physics</jtitle><addtitle>Int J Radiat Oncol Biol Phys</addtitle><date>1998-03-15</date><risdate>1998</risdate><volume>40</volume><issue>5</issue><spage>1019</spage><epage>1026</epage><pages>1019-1026</pages><issn>0360-3016</issn><eissn>1879-355X</eissn><coden>IOBPD3</coden><abstract>Purpose: Patients with a history of head and neck irradiation in childhood are at risk to develop thyroid tumors. The aim of this study was to determine if an impairement of DNA strand breaks repair could account for this observation.
Methods and Materials: Circulating unstimulated lymphocytes of a group of 13 patients who developed thyroid tumors after radiotherapy were submitted to the alkaline single-cell gel electrophoresis assay (SCGE or “comet” assay) after
in vitro exposure to 2 and 5 Gy of γ-rays. A control group of 8 healthy donors and 2 cases with a history of neck irradiation who did not develop a thyroid tumor were also analysed. The immediate response was compared to that observed after 15, 30, and 60 min of postexposure incubation periods.
Results: Induction of DNA strand breaks is a dose–dependent process. The SCGE assay parameters did not differ significantly between patients and controls immediately (
t = 0) after irradiation at the two doses used. As compared to healthy donors, a slower kinetics of repair was found in the patients. The proportion of residual damage at 60 min postirradiation was significantly (
p < 0.01) higher in patients than in controls, at both doses analysed. Flow cytometric analysis of apoptosis and p53 protein status studied before and after irradiation showed no apparent relationship with the repair capacity.
Conclusion: This preliminary study suggests that a subgroup of patients who develop thyroid tumors after a history of irradiation are partially defective in the late restitution of
in vitro radiation-induced DNA strand breaks. This deficiency could be a predisposing factor to radiation-associated thyroid tumorigenesis. Detection of susceptible individuals using the simple and rapid comet assay, especially children receiving radiotherapeutic treatment, may allow a preventive surveillance for radiation-associated epithelial thyroid tumor development.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>9539555</pmid><doi>10.1016/S0360-3016(97)00914-0</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0360-3016 |
| ispartof | International journal of radiation oncology, biology, physics, 1998-03, Vol.40 (5), p.1019-1026 |
| issn | 0360-3016 1879-355X |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adult Apoptosis Biological and medical sciences Blood leukocytes Carcinogenesis, carcinogens and anticarcinogens DNA - radiation effects DNA Damage DNA Repair Electrophoresis - methods Endocrinopathies Female Humans Male Malignant tumors Medical sciences Middle Aged Neoplasms, Radiation-Induced - genetics Physical agents Radiation-induced Single cell gel electrophoresis assay Thyroid cancer Thyroid Gland - radiation effects Thyroid Neoplasms - genetics Thyroid. Thyroid axis (diseases) Tumors |
| title | Impaired DNA Repair as Assessed by the “Comet” Assay in Patients with Thyroid Tumors After a History of Radiation Therapy: A Preliminary Study |
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