Quantitative assessment of angiogenesis, perfused blood vessels and endothelial tip cells in the postnatal mouse brain

This protocol uses intravascular Evans blue labeling and Isolectin-B4 endothelial tip cell staining combined with antibody-based protein detection to monitor new blood vessel formation from tip cell selection to functional, perfused blood vessels. During development and in various diseases of the CN...

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Veröffentlicht in:	Nature protocols 2015-01, Vol.10 (1), p.53-74
Hauptverfasser:	Wälchli, Thomas, Mateos, José María, Weinman, Oliver, Babic, Daniela, Regli, Luca, Hoerstrup, Simon P, Gerhardt, Holger, Schwab, Martin E, Vogel, Johannes
Format:	Artikel
Sprache:	eng
Schlagworte:	631/136/2060/16 631/1647/334/1874/345 631/1647/664/1257 Analysis Analytical Chemistry Angiogenesis Animal experimentation Animals Antibodies Biological Techniques Blood vessels Brain Brain - blood supply Brain - cytology Cardiovascular research Cell density Computational Biology/Bioinformatics Endothelial Cells - metabolism Endothelium Evans Blue Filopodia Health aspects Immunofluorescence Laminin Lectins Life Sciences Methods Mice Microarrays Microscopy, Confocal Neovascularization Neovascularization, Physiologic - physiology Organic Chemistry Perfusion Perfusion (Physiology) Perfusion Imaging - methods Physiological aspects Proteins protocol Scanning microscopy
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