Direct common gram-negative bacterial identification from positive blood culture bottles by SELDI-TOF MS

A protein database was constructed and validated with identification rate over 90% for the 4 most common Gram-negative bacteria on agar plates. By protein masses comparison, 120 bacteria of the 4 species from blood culture bottles were identified. The concordance was high (Kappa=0.906) between our m...

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Veröffentlicht in:	Journal of microbiological methods 2014-10, Vol.105, p.116-120
Hauptverfasser:	Xiao, Daiwen, Yang, Yongchang, Jiang, Wei, Zhang, Hangfeng, Liu, Hua, Yu, Hua, Xie, Chunbao, Zhong, Min, Chen, Liang, Huang, Wenfang
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteremia - diagnosis Bacteremia - microbiology Bacteria Bacteriological Techniques - methods Bloodstream infection Gram-Negative Bacteria - chemistry Gram-Negative Bacteria - classification Gram-Negative Bacteria - isolation & purification Gram-Negative Bacterial Infections - diagnosis Gram-Negative Bacterial Infections - microbiology Humans Identification Protein database SELDI-TOF MS Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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