Purification and characterization of phytase from Bacillus subtilis (natto) N-77

An extracellular phytase from Bacillus subtilis (natto) N-77 was purified 322-fold to homogeneity with the specific activity of 8.7 units per mg protein by ultrafiltration, and a combination of Sephadex G-100 and DEAE-Sepharose CL-6B column chromatographies. The molecular weight of the purified enzy...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1992, Vol.56 (8), p.1266-1269
1. Verfasser:	Shimizu, M. (National Federation of Agricultural Cooperative Associations, Sakura, Chiba (Japan). Inst. of Animal Health)
Format:	Artikel
Sprache:	eng
Schlagworte:	ACIDE PHYTIQUE ACIDO FITICO BACILLUS SUBTILIS Biological and medical sciences biosynthesis Biotechnology characterization CHROMATOGRAPHIE EN COLONNE COLUMN CHROMATOGRAPHY CROMATOGRAFIA DE COLUMNA ELECTROFORESIS ELECTROPHORESE ELECTROPHORESIS ENZIMAS ENZYME Enzyme engineering ENZYMES extracellular enzymes Fundamental and applied biological sciences. Psychology Improved methods for extraction and purification of enzymes INHIBICION INHIBITION inhibitors isoelectric points METAL METALES METALS Methods. Procedures. Technologies MOLECULAR WEIGHT PESO MOLECULAR phytase PHYTIC ACID POIDS MOLECULAIRE PROTEINAS PROTEINE PROTEINS PURIFICACION PURIFICATION
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