Properties of endogenous voltage-dependent sodium currents in Xenopus laevis oocytes
Endogenous voltage-dependent sodium currents were recorded using standard 2-microelectrode techniques in Xenopus laevis oocytes. Maximal inward current occurred at −10 mV with an average amplitude of − 279 ± 7 nA and steady-state inactivation was half-maximal at a voltage of −38 ± 0.5 mV. Currents w...
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| Veröffentlicht in: | Journal of neuroscience methods 1992-07, Vol.43 (2), p.189-193 |
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| container_title | Journal of neuroscience methods |
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| creator | Krafte, Douglas S. Volberg, Walter A. |
| description | Endogenous voltage-dependent sodium currents were recorded using standard 2-microelectrode techniques in
Xenopus laevis oocytes. Maximal inward current occurred at −10 mV with an average amplitude of − 279 ± 7 nA and steady-state inactivation was half-maximal at a voltage of −38 ± 0.5 mV. Currents were blocked by low concentrations of tetrodotoxin (TTX) with an IC
50 value of 6 nM. These properties make the endogenous sodium current in
Xenopus oocytes similar to sodium currents expressed following injection of mammalian brain RNA. While endogenous sodium channels have the potential to complicate analysis when using the oocyte expression system, they are only present at significant levels in rare batches of oocytes (< 5%). Our results do stress the need, however, to reproduce results from exogenous expression studies across several batches of oocytes from different donors. |
| doi_str_mv | 10.1016/0165-0270(92)90028-C |
| format | Article |
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Xenopus laevis oocytes. Maximal inward current occurred at −10 mV with an average amplitude of − 279 ± 7 nA and steady-state inactivation was half-maximal at a voltage of −38 ± 0.5 mV. Currents were blocked by low concentrations of tetrodotoxin (TTX) with an IC
50 value of 6 nM. These properties make the endogenous sodium current in
Xenopus oocytes similar to sodium currents expressed following injection of mammalian brain RNA. While endogenous sodium channels have the potential to complicate analysis when using the oocyte expression system, they are only present at significant levels in rare batches of oocytes (< 5%). Our results do stress the need, however, to reproduce results from exogenous expression studies across several batches of oocytes from different donors.</description><identifier>ISSN: 0165-0270</identifier><identifier>EISSN: 1872-678X</identifier><identifier>DOI: 10.1016/0165-0270(92)90028-C</identifier><identifier>PMID: 1383646</identifier><identifier>CODEN: JNMEDT</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Electrophysiology ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Mammalian female genital system ; Microelectrodes ; Morphology. Physiology ; Na channel ; Oocyte ; Oocytes - metabolism ; RNA - pharmacology ; Sodium Channels - drug effects ; Tetrodotoxin ; Tetrodotoxin - pharmacology ; Vertebrates: reproduction ; Xenopus laevis</subject><ispartof>Journal of neuroscience methods, 1992-07, Vol.43 (2), p.189-193</ispartof><rights>1992</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-3c874728658f3278c9b857f7ff8a4929107d1e4a96143f721eb318d6700030f93</citedby><cites>FETCH-LOGICAL-c417t-3c874728658f3278c9b857f7ff8a4929107d1e4a96143f721eb318d6700030f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/016502709290028C$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4392563$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1383646$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Krafte, Douglas S.</creatorcontrib><creatorcontrib>Volberg, Walter A.</creatorcontrib><title>Properties of endogenous voltage-dependent sodium currents in Xenopus laevis oocytes</title><title>Journal of neuroscience methods</title><addtitle>J Neurosci Methods</addtitle><description>Endogenous voltage-dependent sodium currents were recorded using standard 2-microelectrode techniques in
Xenopus laevis oocytes. Maximal inward current occurred at −10 mV with an average amplitude of − 279 ± 7 nA and steady-state inactivation was half-maximal at a voltage of −38 ± 0.5 mV. Currents were blocked by low concentrations of tetrodotoxin (TTX) with an IC
50 value of 6 nM. These properties make the endogenous sodium current in
Xenopus oocytes similar to sodium currents expressed following injection of mammalian brain RNA. While endogenous sodium channels have the potential to complicate analysis when using the oocyte expression system, they are only present at significant levels in rare batches of oocytes (< 5%). Our results do stress the need, however, to reproduce results from exogenous expression studies across several batches of oocytes from different donors.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Electrophysiology</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Mammalian female genital system</subject><subject>Microelectrodes</subject><subject>Morphology. Physiology</subject><subject>Na channel</subject><subject>Oocyte</subject><subject>Oocytes - metabolism</subject><subject>RNA - pharmacology</subject><subject>Sodium Channels - drug effects</subject><subject>Tetrodotoxin</subject><subject>Tetrodotoxin - pharmacology</subject><subject>Vertebrates: reproduction</subject><subject>Xenopus laevis</subject><issn>0165-0270</issn><issn>1872-678X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LKzEUhoMo2qv-A4VZiNy7GM1X87ERpPgFgi4U3IU0cyKR6WRMZgr996a26O4uQkjOc97kPAidEHxBMBGXZU1rTCX-q-k_jTFV9WwHTYiStBZSve2iyQ9ygP7k_IEx5hqLfbRPmGKCiwl6eU6xhzQEyFX0FXRNfIcujrlaxnaw71A30Jdb6IYqxyaMi8qNKZVjrkJXvRW2L3BrYRlKQnSrAfIR2vO2zXC83Q_R6-3Ny-y-fny6e5hdP9aOEznUzCnJJVViqjyjUjk9V1PppffKck01wbIhwK0WhDMvKYE5I6oRsszBsNfsEJ1vcvsUP0fIg1mE7KBtbQdlBEMEUwIrVkC-AV2KOSfwpk9hYdPKEGzWMs3alFmbMpqab5lmVtpOt_njfAHNb9PGXqmfbes2O9v6ZDsX8g_GmaZTsX79aoNBcbEMkEx2AToHTUjgBtPE8P9_fAFARpAA</recordid><startdate>19920701</startdate><enddate>19920701</enddate><creator>Krafte, Douglas S.</creator><creator>Volberg, Walter A.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>19920701</creationdate><title>Properties of endogenous voltage-dependent sodium currents in Xenopus laevis oocytes</title><author>Krafte, Douglas S. ; Volberg, Walter A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-3c874728658f3278c9b857f7ff8a4929107d1e4a96143f721eb318d6700030f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Electrophysiology</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Mammalian female genital system</topic><topic>Microelectrodes</topic><topic>Morphology. Physiology</topic><topic>Na channel</topic><topic>Oocyte</topic><topic>Oocytes - metabolism</topic><topic>RNA - pharmacology</topic><topic>Sodium Channels - drug effects</topic><topic>Tetrodotoxin</topic><topic>Tetrodotoxin - pharmacology</topic><topic>Vertebrates: reproduction</topic><topic>Xenopus laevis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Krafte, Douglas S.</creatorcontrib><creatorcontrib>Volberg, Walter A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Journal of neuroscience methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krafte, Douglas S.</au><au>Volberg, Walter A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Properties of endogenous voltage-dependent sodium currents in Xenopus laevis oocytes</atitle><jtitle>Journal of neuroscience methods</jtitle><addtitle>J Neurosci Methods</addtitle><date>1992-07-01</date><risdate>1992</risdate><volume>43</volume><issue>2</issue><spage>189</spage><epage>193</epage><pages>189-193</pages><issn>0165-0270</issn><eissn>1872-678X</eissn><coden>JNMEDT</coden><abstract>Endogenous voltage-dependent sodium currents were recorded using standard 2-microelectrode techniques in
Xenopus laevis oocytes. Maximal inward current occurred at −10 mV with an average amplitude of − 279 ± 7 nA and steady-state inactivation was half-maximal at a voltage of −38 ± 0.5 mV. Currents were blocked by low concentrations of tetrodotoxin (TTX) with an IC
50 value of 6 nM. These properties make the endogenous sodium current in
Xenopus oocytes similar to sodium currents expressed following injection of mammalian brain RNA. While endogenous sodium channels have the potential to complicate analysis when using the oocyte expression system, they are only present at significant levels in rare batches of oocytes (< 5%). Our results do stress the need, however, to reproduce results from exogenous expression studies across several batches of oocytes from different donors.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>1383646</pmid><doi>10.1016/0165-0270(92)90028-C</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Electrophysiology Freshwater Fundamental and applied biological sciences. Psychology Mammalian female genital system Microelectrodes Morphology. Physiology Na channel Oocyte Oocytes - metabolism RNA - pharmacology Sodium Channels - drug effects Tetrodotoxin Tetrodotoxin - pharmacology Vertebrates: reproduction Xenopus laevis |
| title | Properties of endogenous voltage-dependent sodium currents in Xenopus laevis oocytes |
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