Tandem DctD‐binding sites of the Rhizobium meliloti dctA upstream activating sequence are essential for optimal function despite a 50‐ to 100‐fold difference in affinity for DctD

Summary The Rhizobium melilot genes dctB and dctD positively regulate the expression of dctA, which encodes a C4‐dicarboxylate transport protein. Here we characterize an element (UAS) located upstream of dctA that has tandem binding sites for the dctD gene product (DctD). At relatively low concentra...

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Veröffentlicht in:	Molecular microbiology 1992-12, Vol.6 (23), p.3479-3492
Hauptverfasser:	Ledebur, H., Nixon, B. T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriology Base Sequence Biological and medical sciences Carrier Proteins - genetics Dicarboxylic Acid Transporters DNA-Binding Proteins - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial Genetics Microbiology Molecular Sequence Data Mutagenesis, Site-Directed Oligodeoxyribonucleotides - chemistry Promoter Regions, Genetic Repetitive Sequences, Nucleic Acid Rhizobium meliloti Sigma Factor - metabolism Sinorhizobium meliloti - genetics Transcription Factors Transcription, Genetic
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