Interferon alpha-inducible protein 27 promotes epithelial–mesenchymal transition and induces ovarian tumorigenicity and stemness
Abstract Background Interferon alpha-inducible protein 27 (IFI27) is an interferon alpha-inducible protein, which was found to be upregulated in some cancers, such as breast cancer, squamous cell carcinoma, hepatocellular carcinoma, and serous ovarian carcinoma. However, the role of IFI27 in ovarian...
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| Veröffentlicht in: | The Journal of surgical research 2015-01, Vol.193 (1), p.255-264 |
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| creator | Li, Shuqin, MD Xie, Yan, MD Zhang, Wei, MD Gao, Junfeng, MD Wang, Man, MD Zheng, Guoxuan, MD Yin, Xing, MD Xia, Hongping, MD, PhD Tao, Xiang, MD, PhD |
| description | Abstract Background Interferon alpha-inducible protein 27 (IFI27) is an interferon alpha-inducible protein, which was found to be upregulated in some cancers, such as breast cancer, squamous cell carcinoma, hepatocellular carcinoma, and serous ovarian carcinoma. However, the role of IFI27 in ovarian cancer remains to be elucidated. This study was designed to investigate the role of IFI27 in ovarian cancer tumorigenicity. Materials and methods The expression of IFI27 was examined in ovarian cancer tissues and cell lines by real time quantitative reverse transcription polymerase chain reaction and immunohistochemistry. The cell migration and invasion was investigated by wound healing and transwell invasion assay. The epithelial–mesenchymal transition markers were detected by Western blotting and the stemness was evaluated by sphere formation. The tumor growth was examined in the athymic mice model. Results We found that IFI27 is overexpressed in ovarian cancer and associated with patients' survival. Interestingly, we further observed that the expression of IFI27 was associated with the expression of mesenchymal marker vimentin in ovarian cancer. Overexpression of IFI27 induces epithelial–mesenchymal transition and promotes epithelial ovarian cancer cells migration and invasion, tumorigenicity, stemness, and drug resistance. Moreover, overexpression of IFI27 is associated with loss of miR-502 in ovarian cancer. Reexpression of miR-502 inhibits IFI27-induced tumorigenicity, migration, and drug resistance. Conclusions These data suggested that IFI27 may be a potential target for developing novel diagnosis strategies and therapeutic interventions. |
| doi_str_mv | 10.1016/j.jss.2014.06.055 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1637555678</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0022480414006337</els_id><sourcerecordid>1637555678</sourcerecordid><originalsourceid>FETCH-LOGICAL-c544t-aed8e2d8321ebfd93258b4a38386d519537129f6bd59310bcc00d580b025f3693</originalsourceid><addsrcrecordid>eNp9kcGK1TAUhosozp3RB3AjXbppPUmatEUQZNBxYMCFug5pcupNTdNrkg7cnfgKvqFPYjp3dOHCVXLg-3843ymKZwRqAkS8nOopxpoCaWoQNXD-oNgR6HnViZY9LHYAlFZNB81ZcR7jBHnuW_a4OKOcABMN7Iof1z5hGDEsvlTusFeV9WbVdnBYHsKS0PqSttt3zkMs8WDTHp1V7tf3nzNG9Hp_nJUrU1A-2mS3Hm_Ku5bML7cqWOXLtM5LsF_QW23T8Q6JCWePMT4pHo3KRXx6_14Un9-9_XT5vrr5cHV9-eam0rxpUqXQdEhNxyjBYTQ9o7wbGsU61gnDSc9ZS2g_isHwnhEYtAYwvIMBKB-Z6NlF8eLUm5f5tmJMcrZRo3PK47JGSQRrOeei7TJKTqgOS4wBR3kIdlbhKAnITb2cZFYvN_UShMzqc-b5ff06zGj-Jv64zsCrE4B5yVuLQUZtsz80NqBO0iz2v_Wv_0lrZ7NN5b7iEeO0rMFne5LISCXIj9vtt9OTBkAw1rLfbm6s5w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1637555678</pqid></control><display><type>article</type><title>Interferon alpha-inducible protein 27 promotes epithelial–mesenchymal transition and induces ovarian tumorigenicity and stemness</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Li, Shuqin, MD ; Xie, Yan, MD ; Zhang, Wei, MD ; Gao, Junfeng, MD ; Wang, Man, MD ; Zheng, Guoxuan, MD ; Yin, Xing, MD ; Xia, Hongping, MD, PhD ; Tao, Xiang, MD, PhD</creator><creatorcontrib>Li, Shuqin, MD ; Xie, Yan, MD ; Zhang, Wei, MD ; Gao, Junfeng, MD ; Wang, Man, MD ; Zheng, Guoxuan, MD ; Yin, Xing, MD ; Xia, Hongping, MD, PhD ; Tao, Xiang, MD, PhD</creatorcontrib><description>Abstract Background Interferon alpha-inducible protein 27 (IFI27) is an interferon alpha-inducible protein, which was found to be upregulated in some cancers, such as breast cancer, squamous cell carcinoma, hepatocellular carcinoma, and serous ovarian carcinoma. However, the role of IFI27 in ovarian cancer remains to be elucidated. This study was designed to investigate the role of IFI27 in ovarian cancer tumorigenicity. Materials and methods The expression of IFI27 was examined in ovarian cancer tissues and cell lines by real time quantitative reverse transcription polymerase chain reaction and immunohistochemistry. The cell migration and invasion was investigated by wound healing and transwell invasion assay. The epithelial–mesenchymal transition markers were detected by Western blotting and the stemness was evaluated by sphere formation. The tumor growth was examined in the athymic mice model. Results We found that IFI27 is overexpressed in ovarian cancer and associated with patients' survival. Interestingly, we further observed that the expression of IFI27 was associated with the expression of mesenchymal marker vimentin in ovarian cancer. Overexpression of IFI27 induces epithelial–mesenchymal transition and promotes epithelial ovarian cancer cells migration and invasion, tumorigenicity, stemness, and drug resistance. Moreover, overexpression of IFI27 is associated with loss of miR-502 in ovarian cancer. Reexpression of miR-502 inhibits IFI27-induced tumorigenicity, migration, and drug resistance. Conclusions These data suggested that IFI27 may be a potential target for developing novel diagnosis strategies and therapeutic interventions.</description><identifier>ISSN: 0022-4804</identifier><identifier>EISSN: 1095-8673</identifier><identifier>DOI: 10.1016/j.jss.2014.06.055</identifier><identifier>PMID: 25103640</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Biomarkers, Tumor - metabolism ; Carcinogenesis - genetics ; Carcinogenesis - metabolism ; Cell Line, Tumor ; Cell Movement - physiology ; Drug Resistance, Neoplasm ; Epithelial-Mesenchymal Transition - physiology ; Epithelial–mesenchymal transition ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; IFI27 ; Interferon-alpha - metabolism ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Metastasis ; Mice, Nude ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miR-502 ; Neoplasm Invasiveness ; Neoplasm Transplantation ; Ovarian cancer ; Ovarian Neoplasms - pathology ; Surgery ; Vimentin - metabolism</subject><ispartof>The Journal of surgical research, 2015-01, Vol.193 (1), p.255-264</ispartof><rights>Elsevier Inc.</rights><rights>2015 Elsevier Inc.</rights><rights>Copyright © 2015 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c544t-aed8e2d8321ebfd93258b4a38386d519537129f6bd59310bcc00d580b025f3693</citedby><cites>FETCH-LOGICAL-c544t-aed8e2d8321ebfd93258b4a38386d519537129f6bd59310bcc00d580b025f3693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jss.2014.06.055$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25103640$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Shuqin, MD</creatorcontrib><creatorcontrib>Xie, Yan, MD</creatorcontrib><creatorcontrib>Zhang, Wei, MD</creatorcontrib><creatorcontrib>Gao, Junfeng, MD</creatorcontrib><creatorcontrib>Wang, Man, MD</creatorcontrib><creatorcontrib>Zheng, Guoxuan, MD</creatorcontrib><creatorcontrib>Yin, Xing, MD</creatorcontrib><creatorcontrib>Xia, Hongping, MD, PhD</creatorcontrib><creatorcontrib>Tao, Xiang, MD, PhD</creatorcontrib><title>Interferon alpha-inducible protein 27 promotes epithelial–mesenchymal transition and induces ovarian tumorigenicity and stemness</title><title>The Journal of surgical research</title><addtitle>J Surg Res</addtitle><description>Abstract Background Interferon alpha-inducible protein 27 (IFI27) is an interferon alpha-inducible protein, which was found to be upregulated in some cancers, such as breast cancer, squamous cell carcinoma, hepatocellular carcinoma, and serous ovarian carcinoma. However, the role of IFI27 in ovarian cancer remains to be elucidated. This study was designed to investigate the role of IFI27 in ovarian cancer tumorigenicity. Materials and methods The expression of IFI27 was examined in ovarian cancer tissues and cell lines by real time quantitative reverse transcription polymerase chain reaction and immunohistochemistry. The cell migration and invasion was investigated by wound healing and transwell invasion assay. The epithelial–mesenchymal transition markers were detected by Western blotting and the stemness was evaluated by sphere formation. The tumor growth was examined in the athymic mice model. Results We found that IFI27 is overexpressed in ovarian cancer and associated with patients' survival. Interestingly, we further observed that the expression of IFI27 was associated with the expression of mesenchymal marker vimentin in ovarian cancer. Overexpression of IFI27 induces epithelial–mesenchymal transition and promotes epithelial ovarian cancer cells migration and invasion, tumorigenicity, stemness, and drug resistance. Moreover, overexpression of IFI27 is associated with loss of miR-502 in ovarian cancer. Reexpression of miR-502 inhibits IFI27-induced tumorigenicity, migration, and drug resistance. Conclusions These data suggested that IFI27 may be a potential target for developing novel diagnosis strategies and therapeutic interventions.</description><subject>Animals</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Carcinogenesis - genetics</subject><subject>Carcinogenesis - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - physiology</subject><subject>Drug Resistance, Neoplasm</subject><subject>Epithelial-Mesenchymal Transition - physiology</subject><subject>Epithelial–mesenchymal transition</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>IFI27</subject><subject>Interferon-alpha - metabolism</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Metastasis</subject><subject>Mice, Nude</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miR-502</subject><subject>Neoplasm Invasiveness</subject><subject>Neoplasm Transplantation</subject><subject>Ovarian cancer</subject><subject>Ovarian Neoplasms - pathology</subject><subject>Surgery</subject><subject>Vimentin - metabolism</subject><issn>0022-4804</issn><issn>1095-8673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcGK1TAUhosozp3RB3AjXbppPUmatEUQZNBxYMCFug5pcupNTdNrkg7cnfgKvqFPYjp3dOHCVXLg-3843ymKZwRqAkS8nOopxpoCaWoQNXD-oNgR6HnViZY9LHYAlFZNB81ZcR7jBHnuW_a4OKOcABMN7Iof1z5hGDEsvlTusFeV9WbVdnBYHsKS0PqSttt3zkMs8WDTHp1V7tf3nzNG9Hp_nJUrU1A-2mS3Hm_Ku5bML7cqWOXLtM5LsF_QW23T8Q6JCWePMT4pHo3KRXx6_14Un9-9_XT5vrr5cHV9-eam0rxpUqXQdEhNxyjBYTQ9o7wbGsU61gnDSc9ZS2g_isHwnhEYtAYwvIMBKB-Z6NlF8eLUm5f5tmJMcrZRo3PK47JGSQRrOeei7TJKTqgOS4wBR3kIdlbhKAnITb2cZFYvN_UShMzqc-b5ff06zGj-Jv64zsCrE4B5yVuLQUZtsz80NqBO0iz2v_Wv_0lrZ7NN5b7iEeO0rMFne5LISCXIj9vtt9OTBkAw1rLfbm6s5w</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Li, Shuqin, MD</creator><creator>Xie, Yan, MD</creator><creator>Zhang, Wei, MD</creator><creator>Gao, Junfeng, MD</creator><creator>Wang, Man, MD</creator><creator>Zheng, Guoxuan, MD</creator><creator>Yin, Xing, MD</creator><creator>Xia, Hongping, MD, PhD</creator><creator>Tao, Xiang, MD, PhD</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150101</creationdate><title>Interferon alpha-inducible protein 27 promotes epithelial–mesenchymal transition and induces ovarian tumorigenicity and stemness</title><author>Li, Shuqin, MD ; Xie, Yan, MD ; Zhang, Wei, MD ; Gao, Junfeng, MD ; Wang, Man, MD ; Zheng, Guoxuan, MD ; Yin, Xing, MD ; Xia, Hongping, MD, PhD ; Tao, Xiang, MD, PhD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c544t-aed8e2d8321ebfd93258b4a38386d519537129f6bd59310bcc00d580b025f3693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Carcinogenesis - genetics</topic><topic>Carcinogenesis - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement - physiology</topic><topic>Drug Resistance, Neoplasm</topic><topic>Epithelial-Mesenchymal Transition - physiology</topic><topic>Epithelial–mesenchymal transition</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>IFI27</topic><topic>Interferon-alpha - metabolism</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - metabolism</topic><topic>Metastasis</topic><topic>Mice, Nude</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>miR-502</topic><topic>Neoplasm Invasiveness</topic><topic>Neoplasm Transplantation</topic><topic>Ovarian cancer</topic><topic>Ovarian Neoplasms - pathology</topic><topic>Surgery</topic><topic>Vimentin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Shuqin, MD</creatorcontrib><creatorcontrib>Xie, Yan, MD</creatorcontrib><creatorcontrib>Zhang, Wei, MD</creatorcontrib><creatorcontrib>Gao, Junfeng, MD</creatorcontrib><creatorcontrib>Wang, Man, MD</creatorcontrib><creatorcontrib>Zheng, Guoxuan, MD</creatorcontrib><creatorcontrib>Yin, Xing, MD</creatorcontrib><creatorcontrib>Xia, Hongping, MD, PhD</creatorcontrib><creatorcontrib>Tao, Xiang, MD, PhD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of surgical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Shuqin, MD</au><au>Xie, Yan, MD</au><au>Zhang, Wei, MD</au><au>Gao, Junfeng, MD</au><au>Wang, Man, MD</au><au>Zheng, Guoxuan, MD</au><au>Yin, Xing, MD</au><au>Xia, Hongping, MD, PhD</au><au>Tao, Xiang, MD, PhD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interferon alpha-inducible protein 27 promotes epithelial–mesenchymal transition and induces ovarian tumorigenicity and stemness</atitle><jtitle>The Journal of surgical research</jtitle><addtitle>J Surg Res</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>193</volume><issue>1</issue><spage>255</spage><epage>264</epage><pages>255-264</pages><issn>0022-4804</issn><eissn>1095-8673</eissn><abstract>Abstract Background Interferon alpha-inducible protein 27 (IFI27) is an interferon alpha-inducible protein, which was found to be upregulated in some cancers, such as breast cancer, squamous cell carcinoma, hepatocellular carcinoma, and serous ovarian carcinoma. However, the role of IFI27 in ovarian cancer remains to be elucidated. This study was designed to investigate the role of IFI27 in ovarian cancer tumorigenicity. Materials and methods The expression of IFI27 was examined in ovarian cancer tissues and cell lines by real time quantitative reverse transcription polymerase chain reaction and immunohistochemistry. The cell migration and invasion was investigated by wound healing and transwell invasion assay. The epithelial–mesenchymal transition markers were detected by Western blotting and the stemness was evaluated by sphere formation. The tumor growth was examined in the athymic mice model. Results We found that IFI27 is overexpressed in ovarian cancer and associated with patients' survival. Interestingly, we further observed that the expression of IFI27 was associated with the expression of mesenchymal marker vimentin in ovarian cancer. Overexpression of IFI27 induces epithelial–mesenchymal transition and promotes epithelial ovarian cancer cells migration and invasion, tumorigenicity, stemness, and drug resistance. Moreover, overexpression of IFI27 is associated with loss of miR-502 in ovarian cancer. Reexpression of miR-502 inhibits IFI27-induced tumorigenicity, migration, and drug resistance. Conclusions These data suggested that IFI27 may be a potential target for developing novel diagnosis strategies and therapeutic interventions.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>25103640</pmid><doi>10.1016/j.jss.2014.06.055</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Biomarkers, Tumor - metabolism Carcinogenesis - genetics Carcinogenesis - metabolism Cell Line, Tumor Cell Movement - physiology Drug Resistance, Neoplasm Epithelial-Mesenchymal Transition - physiology Epithelial–mesenchymal transition Female Gene Expression Regulation, Neoplastic Humans IFI27 Interferon-alpha - metabolism Membrane Proteins - genetics Membrane Proteins - metabolism Metastasis Mice, Nude MicroRNAs - genetics MicroRNAs - metabolism miR-502 Neoplasm Invasiveness Neoplasm Transplantation Ovarian cancer Ovarian Neoplasms - pathology Surgery Vimentin - metabolism |
| title | Interferon alpha-inducible protein 27 promotes epithelial–mesenchymal transition and induces ovarian tumorigenicity and stemness |
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