Identification and cloning of a mobile transposon from Aspergillus niger var. awamori
Aspergillus niger var. awamori contains multiple copies of a transposable element, Vader. This element was detected as a 437-bp insertion in four independently isolated spontaneous mutants of the niaD (nitrate reductase) gene. The Vader element is present in approximately 15 copies in both A. niger...
Gespeichert in:
| Veröffentlicht in: | Current genetics 1996-04, Vol.29 (5), p.468-473 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 473 |
|---|---|
| container_issue | 5 |
| container_start_page | 468 |
| container_title | Current genetics |
| container_volume | 29 |
| creator | Amutan, M Nyyssoenen, E Stubbs, J Diaz-Torres, M Dunn-Coleman, N |
| description | Aspergillus niger var. awamori contains multiple copies of a transposable element, Vader. This element was detected as a 437-bp insertion in four independently isolated spontaneous mutants of the niaD (nitrate reductase) gene. The Vader element is present in approximately 15 copies in both A. niger var. awamori and A. niger. A single copy of Vader was detected from only one of the two laboratory strains of A. nidulans which were also examined. Insertion of the Vader element into the niaD gene of A. niger var. awamori caused a 2-bp duplication (TA) of the target sequence. The Vader element is flanked by a 44-bp inverted repeat. The genetic stabilities of the inserted Vader elements at niaD were examined by studying reversion frequencies resulting in colonies able to grow on nitrate as a sole nitrogen source. Mutants niaD392 and niaD436 reverted at a frequency of 9x10(-3) and 4x10(-2), respectively. Two of the mutants, niaD587 and niaD410, reverted at a lower frequency of 6x10(-4). |
| doi_str_mv | 10.1007/BF02221516 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_16372644</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>16372644</sourcerecordid><originalsourceid>FETCH-LOGICAL-f260t-95059efbca4a4399f386de1fbd007ec546c7f1cb9387926dfb447b46831e4a3a3</originalsourceid><addsrcrecordid>eNotkDtPwzAYRS0EKqWwsIM8saX4FT_GUlqoVImFztGXxI6MkrjYCYh_T6R2usM5utK9CN1TsqSEqOeXLWGM0ZzKCzSngrOMGM0v0ZxQxTJNNL9GNyl9EUKZNmqGZlqyXDA1R4ddbfvBO1_B4EOPoa9x1Ybe9w0ODgPuQulbi4cIfTqGNCkuhg6v0tHGxrftmHDvGxvxD8Qlhl_oQvS36MpBm-zdORfosN18rt-z_cfbbr3aZ45JMmQmJ7mxrqxAgODGOK5lbakr62mVrXIhK-VoVRqulWGydqUQqhRSc2oFcOAL9HTqPcbwPdo0FJ1PlW1b6G0YU0ElV0wKMYkPZ3EsO1sXx-g7iH_F-YeJP564g1BAE30qXjfUGEWI1pJq_g8vuGep</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16372644</pqid></control><display><type>article</type><title>Identification and cloning of a mobile transposon from Aspergillus niger var. awamori</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Amutan, M ; Nyyssoenen, E ; Stubbs, J ; Diaz-Torres, M ; Dunn-Coleman, N</creator><creatorcontrib>Amutan, M ; Nyyssoenen, E ; Stubbs, J ; Diaz-Torres, M ; Dunn-Coleman, N ; Genencor International, San Francisco (USA). Microbiology Dept</creatorcontrib><description>Aspergillus niger var. awamori contains multiple copies of a transposable element, Vader. This element was detected as a 437-bp insertion in four independently isolated spontaneous mutants of the niaD (nitrate reductase) gene. The Vader element is present in approximately 15 copies in both A. niger var. awamori and A. niger. A single copy of Vader was detected from only one of the two laboratory strains of A. nidulans which were also examined. Insertion of the Vader element into the niaD gene of A. niger var. awamori caused a 2-bp duplication (TA) of the target sequence. The Vader element is flanked by a 44-bp inverted repeat. The genetic stabilities of the inserted Vader elements at niaD were examined by studying reversion frequencies resulting in colonies able to grow on nitrate as a sole nitrogen source. Mutants niaD392 and niaD436 reverted at a frequency of 9x10(-3) and 4x10(-2), respectively. Two of the mutants, niaD587 and niaD410, reverted at a lower frequency of 6x10(-4).</description><identifier>ISSN: 0172-8083</identifier><identifier>EISSN: 1432-0983</identifier><identifier>DOI: 10.1007/BF02221516</identifier><identifier>PMID: 8625427</identifier><language>eng</language><publisher>United States</publisher><subject>aspergillus niger ; Aspergillus niger - genetics ; Base Sequence ; Blotting, Southern ; clonacion molecular ; clonage moleculaire ; DNA Transposable Elements ; gene ; Gene Dosage ; genes ; molecular cloning ; Molecular Sequence Data ; mutacion ; Mutagenesis ; mutation ; Nitrate Reductase ; Nitrate Reductases - analysis ; Nitrate Reductases - genetics ; nitrato reductasa ; Polymerase Chain Reaction ; Selection, Genetic ; Sequence Analysis, DNA ; Species Specificity</subject><ispartof>Current genetics, 1996-04, Vol.29 (5), p.468-473</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8625427$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Amutan, M</creatorcontrib><creatorcontrib>Nyyssoenen, E</creatorcontrib><creatorcontrib>Stubbs, J</creatorcontrib><creatorcontrib>Diaz-Torres, M</creatorcontrib><creatorcontrib>Dunn-Coleman, N</creatorcontrib><creatorcontrib>Genencor International, San Francisco (USA). Microbiology Dept</creatorcontrib><title>Identification and cloning of a mobile transposon from Aspergillus niger var. awamori</title><title>Current genetics</title><addtitle>Curr Genet</addtitle><description>Aspergillus niger var. awamori contains multiple copies of a transposable element, Vader. This element was detected as a 437-bp insertion in four independently isolated spontaneous mutants of the niaD (nitrate reductase) gene. The Vader element is present in approximately 15 copies in both A. niger var. awamori and A. niger. A single copy of Vader was detected from only one of the two laboratory strains of A. nidulans which were also examined. Insertion of the Vader element into the niaD gene of A. niger var. awamori caused a 2-bp duplication (TA) of the target sequence. The Vader element is flanked by a 44-bp inverted repeat. The genetic stabilities of the inserted Vader elements at niaD were examined by studying reversion frequencies resulting in colonies able to grow on nitrate as a sole nitrogen source. Mutants niaD392 and niaD436 reverted at a frequency of 9x10(-3) and 4x10(-2), respectively. Two of the mutants, niaD587 and niaD410, reverted at a lower frequency of 6x10(-4).</description><subject>aspergillus niger</subject><subject>Aspergillus niger - genetics</subject><subject>Base Sequence</subject><subject>Blotting, Southern</subject><subject>clonacion molecular</subject><subject>clonage moleculaire</subject><subject>DNA Transposable Elements</subject><subject>gene</subject><subject>Gene Dosage</subject><subject>genes</subject><subject>molecular cloning</subject><subject>Molecular Sequence Data</subject><subject>mutacion</subject><subject>Mutagenesis</subject><subject>mutation</subject><subject>Nitrate Reductase</subject><subject>Nitrate Reductases - analysis</subject><subject>Nitrate Reductases - genetics</subject><subject>nitrato reductasa</subject><subject>Polymerase Chain Reaction</subject><subject>Selection, Genetic</subject><subject>Sequence Analysis, DNA</subject><subject>Species Specificity</subject><issn>0172-8083</issn><issn>1432-0983</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNotkDtPwzAYRS0EKqWwsIM8saX4FT_GUlqoVImFztGXxI6MkrjYCYh_T6R2usM5utK9CN1TsqSEqOeXLWGM0ZzKCzSngrOMGM0v0ZxQxTJNNL9GNyl9EUKZNmqGZlqyXDA1R4ddbfvBO1_B4EOPoa9x1Ybe9w0ODgPuQulbi4cIfTqGNCkuhg6v0tHGxrftmHDvGxvxD8Qlhl_oQvS36MpBm-zdORfosN18rt-z_cfbbr3aZ45JMmQmJ7mxrqxAgODGOK5lbakr62mVrXIhK-VoVRqulWGydqUQqhRSc2oFcOAL9HTqPcbwPdo0FJ1PlW1b6G0YU0ElV0wKMYkPZ3EsO1sXx-g7iH_F-YeJP564g1BAE30qXjfUGEWI1pJq_g8vuGep</recordid><startdate>19960401</startdate><enddate>19960401</enddate><creator>Amutan, M</creator><creator>Nyyssoenen, E</creator><creator>Stubbs, J</creator><creator>Diaz-Torres, M</creator><creator>Dunn-Coleman, N</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19960401</creationdate><title>Identification and cloning of a mobile transposon from Aspergillus niger var. awamori</title><author>Amutan, M ; Nyyssoenen, E ; Stubbs, J ; Diaz-Torres, M ; Dunn-Coleman, N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f260t-95059efbca4a4399f386de1fbd007ec546c7f1cb9387926dfb447b46831e4a3a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>aspergillus niger</topic><topic>Aspergillus niger - genetics</topic><topic>Base Sequence</topic><topic>Blotting, Southern</topic><topic>clonacion molecular</topic><topic>clonage moleculaire</topic><topic>DNA Transposable Elements</topic><topic>gene</topic><topic>Gene Dosage</topic><topic>genes</topic><topic>molecular cloning</topic><topic>Molecular Sequence Data</topic><topic>mutacion</topic><topic>Mutagenesis</topic><topic>mutation</topic><topic>Nitrate Reductase</topic><topic>Nitrate Reductases - analysis</topic><topic>Nitrate Reductases - genetics</topic><topic>nitrato reductasa</topic><topic>Polymerase Chain Reaction</topic><topic>Selection, Genetic</topic><topic>Sequence Analysis, DNA</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Amutan, M</creatorcontrib><creatorcontrib>Nyyssoenen, E</creatorcontrib><creatorcontrib>Stubbs, J</creatorcontrib><creatorcontrib>Diaz-Torres, M</creatorcontrib><creatorcontrib>Dunn-Coleman, N</creatorcontrib><creatorcontrib>Genencor International, San Francisco (USA). Microbiology Dept</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Current genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Amutan, M</au><au>Nyyssoenen, E</au><au>Stubbs, J</au><au>Diaz-Torres, M</au><au>Dunn-Coleman, N</au><aucorp>Genencor International, San Francisco (USA). Microbiology Dept</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and cloning of a mobile transposon from Aspergillus niger var. awamori</atitle><jtitle>Current genetics</jtitle><addtitle>Curr Genet</addtitle><date>1996-04-01</date><risdate>1996</risdate><volume>29</volume><issue>5</issue><spage>468</spage><epage>473</epage><pages>468-473</pages><issn>0172-8083</issn><eissn>1432-0983</eissn><abstract>Aspergillus niger var. awamori contains multiple copies of a transposable element, Vader. This element was detected as a 437-bp insertion in four independently isolated spontaneous mutants of the niaD (nitrate reductase) gene. The Vader element is present in approximately 15 copies in both A. niger var. awamori and A. niger. A single copy of Vader was detected from only one of the two laboratory strains of A. nidulans which were also examined. Insertion of the Vader element into the niaD gene of A. niger var. awamori caused a 2-bp duplication (TA) of the target sequence. The Vader element is flanked by a 44-bp inverted repeat. The genetic stabilities of the inserted Vader elements at niaD were examined by studying reversion frequencies resulting in colonies able to grow on nitrate as a sole nitrogen source. Mutants niaD392 and niaD436 reverted at a frequency of 9x10(-3) and 4x10(-2), respectively. Two of the mutants, niaD587 and niaD410, reverted at a lower frequency of 6x10(-4).</abstract><cop>United States</cop><pmid>8625427</pmid><doi>10.1007/BF02221516</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0172-8083 |
| ispartof | Current genetics, 1996-04, Vol.29 (5), p.468-473 |
| issn | 0172-8083 1432-0983 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16372644 |
| source | MEDLINE; SpringerLink Journals |
| subjects | aspergillus niger Aspergillus niger - genetics Base Sequence Blotting, Southern clonacion molecular clonage moleculaire DNA Transposable Elements gene Gene Dosage genes molecular cloning Molecular Sequence Data mutacion Mutagenesis mutation Nitrate Reductase Nitrate Reductases - analysis Nitrate Reductases - genetics nitrato reductasa Polymerase Chain Reaction Selection, Genetic Sequence Analysis, DNA Species Specificity |
| title | Identification and cloning of a mobile transposon from Aspergillus niger var. awamori |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T05%3A45%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20and%20cloning%20of%20a%20mobile%20transposon%20from%20Aspergillus%20niger%20var.%20awamori&rft.jtitle=Current%20genetics&rft.au=Amutan,%20M&rft.aucorp=Genencor%20International,%20San%20Francisco%20(USA).%20Microbiology%20Dept&rft.date=1996-04-01&rft.volume=29&rft.issue=5&rft.spage=468&rft.epage=473&rft.pages=468-473&rft.issn=0172-8083&rft.eissn=1432-0983&rft_id=info:doi/10.1007/BF02221516&rft_dat=%3Cproquest_pubme%3E16372644%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16372644&rft_id=info:pmid/8625427&rfr_iscdi=true |