An antisense chalcone synthase cDNA leads to novel colour patterns in lisianthus (Eustoma grandiflorum) flowers
Three cultivars of lisianthus (Eustoma grandiflorum (Grise.)) were transformed with a homologous antisense CHS cDNA via Agrobacterium-mediated transformation. Over 50% of the transgenics derived from the purple flowering lines exhibited an altered flower colour pattern ranging from small streaks of...
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| Veröffentlicht in: | Molecular breeding 1998-01, Vol.4 (1), p.59-66 |
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| creator | Deroles, S.C. (New Zealand Inst. for Crop and Food Research Ltd., Levin (New Zealand). Levin Research Centre) Bradley, J.M Schwinn, K.E Markham, K.R Bloor, S Manson, D.G Davies, K.M |
| description | Three cultivars of lisianthus (Eustoma grandiflorum (Grise.)) were transformed with a homologous antisense CHS cDNA via Agrobacterium-mediated transformation. Over 50% of the transgenics derived from the purple flowering lines exhibited an altered flower colour pattern ranging from small streaks of white on the wild-type purple background through to completely white flowers. A significant portion of the transgenic lines showed unstable phenotypes. Northern and biochemical analysis showed that the altered flower patterns were associated with a loss of CHS gene transcript and a corresponding loss of CHS enzyme activity. In the white flowering line the level of total flavonoids was reduced to ca. 2.0% of the wild-type level. Some of the transgenic plants also exhibited alterations in flower form such as the formation of frilled petal tips and reduced flower opening. Several of the new patterned lines are being evaluated for stability and possible commercial release. |
| doi_str_mv | 10.1023/A:1009621903402 |
| format | Article |
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(New Zealand Inst. for Crop and Food Research Ltd., Levin (New Zealand). Levin Research Centre) ; Bradley, J.M ; Schwinn, K.E ; Markham, K.R ; Bloor, S ; Manson, D.G ; Davies, K.M</creator><creatorcontrib>Deroles, S.C. (New Zealand Inst. for Crop and Food Research Ltd., Levin (New Zealand). Levin Research Centre) ; Bradley, J.M ; Schwinn, K.E ; Markham, K.R ; Bloor, S ; Manson, D.G ; Davies, K.M</creatorcontrib><description>Three cultivars of lisianthus (Eustoma grandiflorum (Grise.)) were transformed with a homologous antisense CHS cDNA via Agrobacterium-mediated transformation. Over 50% of the transgenics derived from the purple flowering lines exhibited an altered flower colour pattern ranging from small streaks of white on the wild-type purple background through to completely white flowers. A significant portion of the transgenic lines showed unstable phenotypes. Northern and biochemical analysis showed that the altered flower patterns were associated with a loss of CHS gene transcript and a corresponding loss of CHS enzyme activity. In the white flowering line the level of total flavonoids was reduced to ca. 2.0% of the wild-type level. Some of the transgenic plants also exhibited alterations in flower form such as the formation of frilled petal tips and reduced flower opening. Several of the new patterned lines are being evaluated for stability and possible commercial release.</description><identifier>ISSN: 1380-3743</identifier><identifier>EISSN: 1572-9788</identifier><identifier>DOI: 10.1023/A:1009621903402</identifier><language>eng</language><publisher>Dordrecht: Springer Nature B.V</publisher><subject>ADN ; AGROBACTERIUM ; Antisense DNA ; Biochemical analysis ; CALCONA ; CHALCONE ; Chalcone synthase ; CHALCONES ; CHS gene ; COLOR ; COLOUR ; COULEUR ; Cultivars ; DNA ; Enzymatic activity ; Enzyme activity ; EUSTOMA GRANDIFLORUM ; Flavonoids ; FLEUR ; FLORES ; Flowering ; FLOWERS ; GENE TRANSFER ; GENETIC TRANSFORMATION ; Homology ; Molecular biology ; Phenotypes ; Plant biology ; PLANTAS TRANSGENICAS ; PLANTE TRANSGENIQUE ; Plants (botany) ; Stability analysis ; Transcription ; TRANSFERENCIA DE GENES ; TRANSFERT DE GENE ; TRANSFORMACION GENETICA ; TRANSFORMATION GENETIQUE ; TRANSGENIC PLANTS</subject><ispartof>Molecular breeding, 1998-01, Vol.4 (1), p.59-66</ispartof><rights>Molecular Breeding is a copyright of Springer, (1998). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c280t-9e88be7acb2f9922289a6b457ee1e7d140ef404c32876b3f0b2b4619adc2efc83</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Deroles, S.C. (New Zealand Inst. for Crop and Food Research Ltd., Levin (New Zealand). 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Northern and biochemical analysis showed that the altered flower patterns were associated with a loss of CHS gene transcript and a corresponding loss of CHS enzyme activity. In the white flowering line the level of total flavonoids was reduced to ca. 2.0% of the wild-type level. Some of the transgenic plants also exhibited alterations in flower form such as the formation of frilled petal tips and reduced flower opening. Several of the new patterned lines are being evaluated for stability and possible commercial release.</description><subject>ADN</subject><subject>AGROBACTERIUM</subject><subject>Antisense DNA</subject><subject>Biochemical analysis</subject><subject>CALCONA</subject><subject>CHALCONE</subject><subject>Chalcone synthase</subject><subject>CHALCONES</subject><subject>CHS gene</subject><subject>COLOR</subject><subject>COLOUR</subject><subject>COULEUR</subject><subject>Cultivars</subject><subject>DNA</subject><subject>Enzymatic activity</subject><subject>Enzyme activity</subject><subject>EUSTOMA GRANDIFLORUM</subject><subject>Flavonoids</subject><subject>FLEUR</subject><subject>FLORES</subject><subject>Flowering</subject><subject>FLOWERS</subject><subject>GENE TRANSFER</subject><subject>GENETIC TRANSFORMATION</subject><subject>Homology</subject><subject>Molecular biology</subject><subject>Phenotypes</subject><subject>Plant biology</subject><subject>PLANTAS TRANSGENICAS</subject><subject>PLANTE TRANSGENIQUE</subject><subject>Plants (botany)</subject><subject>Stability analysis</subject><subject>Transcription</subject><subject>TRANSFERENCIA DE GENES</subject><subject>TRANSFERT DE GENE</subject><subject>TRANSFORMACION GENETICA</subject><subject>TRANSFORMATION GENETIQUE</subject><subject>TRANSGENIC PLANTS</subject><issn>1380-3743</issn><issn>1572-9788</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpdkM1LxDAUxIsouK6ePQkBQfRQzVebxFtZ1w9Y1oueS5q-7nbJJmuTKv73RtaTp3kMP4Y3k2XnBN8STNlddU8wViUlCjOO6UE2IYWguRJSHqabSZwzwdlxdhLCBmMsVFlOMl85pF3sA7gAyKy1Nd4BCt8urvWv87CskAXdBhQ9cv4TLDLe-nFAOx0jDC6g3iHbhz7FrMeArudjiH6r0WrQru0764dxe4OSfsEQTrOjTtsAZ386zd4f52-z53zx-vQyqxa5oRLHXIGUDQhtGtopRSmVSpcNLwQAAdESjqHjmBtGpSgb1uGGNrwkSreGQmckm2ZX-9zd4D9GCLHe9sGAtdqBH0NNSiYoZTyBl__ATSrn0m81pYXiqiiFStTFnuq0r_Vq6EO9XBClJMZpVMV-AKiecwU</recordid><startdate>19980101</startdate><enddate>19980101</enddate><creator>Deroles, S.C. 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(New Zealand Inst. for Crop and Food Research Ltd., Levin (New Zealand). 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(New Zealand Inst. for Crop and Food Research Ltd., Levin (New Zealand). Levin Research Centre)</au><au>Bradley, J.M</au><au>Schwinn, K.E</au><au>Markham, K.R</au><au>Bloor, S</au><au>Manson, D.G</au><au>Davies, K.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An antisense chalcone synthase cDNA leads to novel colour patterns in lisianthus (Eustoma grandiflorum) flowers</atitle><jtitle>Molecular breeding</jtitle><date>1998-01-01</date><risdate>1998</risdate><volume>4</volume><issue>1</issue><spage>59</spage><epage>66</epage><pages>59-66</pages><issn>1380-3743</issn><eissn>1572-9788</eissn><abstract>Three cultivars of lisianthus (Eustoma grandiflorum (Grise.)) were transformed with a homologous antisense CHS cDNA via Agrobacterium-mediated transformation. Over 50% of the transgenics derived from the purple flowering lines exhibited an altered flower colour pattern ranging from small streaks of white on the wild-type purple background through to completely white flowers. A significant portion of the transgenic lines showed unstable phenotypes. Northern and biochemical analysis showed that the altered flower patterns were associated with a loss of CHS gene transcript and a corresponding loss of CHS enzyme activity. In the white flowering line the level of total flavonoids was reduced to ca. 2.0% of the wild-type level. Some of the transgenic plants also exhibited alterations in flower form such as the formation of frilled petal tips and reduced flower opening. Several of the new patterned lines are being evaluated for stability and possible commercial release.</abstract><cop>Dordrecht</cop><pub>Springer Nature B.V</pub><doi>10.1023/A:1009621903402</doi><tpages>8</tpages></addata></record> |
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| subjects | ADN AGROBACTERIUM Antisense DNA Biochemical analysis CALCONA CHALCONE Chalcone synthase CHALCONES CHS gene COLOR COLOUR COULEUR Cultivars DNA Enzymatic activity Enzyme activity EUSTOMA GRANDIFLORUM Flavonoids FLEUR FLORES Flowering FLOWERS GENE TRANSFER GENETIC TRANSFORMATION Homology Molecular biology Phenotypes Plant biology PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE Plants (botany) Stability analysis Transcription TRANSFERENCIA DE GENES TRANSFERT DE GENE TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE TRANSGENIC PLANTS |
| title | An antisense chalcone synthase cDNA leads to novel colour patterns in lisianthus (Eustoma grandiflorum) flowers |
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