ortho-toluidine blood protein adducts: HPLC analysis with fluorescence detection after a single dose in the adult male rat
Hemoglobin (Hb) and albumin (Alb) adducts of the suspect human carcinogen ortho-toluidine (OT) were quantified in blood samples collected from rats after a single i.p. injection. Mild alkaline hydrolysis of Hb adducted with [ 14C]OT followed by extraction with ethyl acetate resulted in recovery of 6...
Gespeichert in:
| Veröffentlicht in: | Fundamental and applied toxicology 1992-05, Vol.18 (4), p.522-531 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 531 |
|---|---|
| container_issue | 4 |
| container_start_page | 522 |
| container_title | Fundamental and applied toxicology |
| container_volume | 18 |
| creator | Cheever, Kenneth L. DeBord, D.Gayle Swearengin, Terri F. Booth-Jones, Angela D. |
| description | Hemoglobin (Hb) and albumin (Alb) adducts of the suspect human carcinogen
ortho-toluidine (OT) were quantified in blood samples collected from rats after a single i.p. injection. Mild alkaline hydrolysis of Hb adducted with [
14C]OT followed by extraction with ethyl acetate resulted in recovery of 63% of the bound radioactivity. HPLC analysis revealed a single radiolabeled peak which was identified as OT by GC-MS. In subsequent experiments Hb and Alb adduct levels were determined by HPLC analysis of this cleavage product using fluorescence detection. 4-Ethylaniline was used as internal standard. The detection limit for OT was approximately 450 pg/injection or 5 pmol/mg Hb. Mean adduct levels for Hb increased rapidly over the first 4 hr with the highest (ng/mg Hb ± SD) 3.7 ± 0.5 detected 24 hr after OT administration at 50 mg/kg body wt. In contrast, adduct levels for pooled Alb samples increased from 0.7 ng/mg Alb at 2 hr to 2.5 ng/mg Alb at 4 hr, but were not detectable 24 hr after dosing. Hb adducts showed a linear relationship for OT doses of 10, 20, 40, 50, and 100 mg/kg body wt. The Hb adduct
t
1
2
(11 days) was determined after a single 100 mg/kg OT dose. Hb adduct levels were quantifiable (1.3 ± 0.2 ng/mg Hb) by HPLC/fluorescence 28 days after 100 mg/kg OT. Although Hb and Alb adducts differ in stability, a ratio of such OT adducts may be useful in long-term industrial biomonitoring for evaluation of OT exposure. |
| doi_str_mv | 10.1016/0272-0590(92)90111-T |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_16363938</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>027205909290111T</els_id><sourcerecordid>16363938</sourcerecordid><originalsourceid>FETCH-LOGICAL-e292t-c5fce7d5eecbf86ebadba9150066cfef8bd507a5be83302f6945b0b3afabd8353</originalsourceid><addsrcrecordid>eNo9UctqHDEQFCbBXj_-wAadgn2YRI_V7CgHg1niByzEh_VZ6NHyKmhHjqRJcL7e2njxqaGruuiqQuickq-U0P4bYQvWESHJpWRXklBKu_UBmlEiRdcPnH1Csw_KETou5RdpJDEnh-iQCtbzfj5D_1Kum9TVFKfgwgjYxJQcfsmpQhixdm6ytXzH94-rJdajjq8lFPw31A32cUoZioXRAnZQwdaQ2omvkLHGJYzPsQGpAG5KdQNNbYoVb3VbZ11P0WevY4Gz_TxBT7c_1sv7bvXz7mF5s-qASVY7K7yFhRMA1vihB6Od0ZIKQvreevCDcYIstDAwcE6Y7-VcGGK49tq4gQt-gr686zZTvycoVW1D-zpGPUKaiqItCS750IgXe-JktuDUSw5bnV_VPqyGX7_j0L79EyCrYsPOvQu5mVcuBUWJ2pWjdsmrXfJKMvW_HLXmb4hFg2Y</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16363938</pqid></control><display><type>article</type><title>ortho-toluidine blood protein adducts: HPLC analysis with fluorescence detection after a single dose in the adult male rat</title><source>MEDLINE</source><source>Oxford Journals A-Z Collection</source><source>Alma/SFX Local Collection</source><creator>Cheever, Kenneth L. ; DeBord, D.Gayle ; Swearengin, Terri F. ; Booth-Jones, Angela D.</creator><creatorcontrib>Cheever, Kenneth L. ; DeBord, D.Gayle ; Swearengin, Terri F. ; Booth-Jones, Angela D.</creatorcontrib><description>Hemoglobin (Hb) and albumin (Alb) adducts of the suspect human carcinogen
ortho-toluidine (OT) were quantified in blood samples collected from rats after a single i.p. injection. Mild alkaline hydrolysis of Hb adducted with [
14C]OT followed by extraction with ethyl acetate resulted in recovery of 63% of the bound radioactivity. HPLC analysis revealed a single radiolabeled peak which was identified as OT by GC-MS. In subsequent experiments Hb and Alb adduct levels were determined by HPLC analysis of this cleavage product using fluorescence detection. 4-Ethylaniline was used as internal standard. The detection limit for OT was approximately 450 pg/injection or 5 pmol/mg Hb. Mean adduct levels for Hb increased rapidly over the first 4 hr with the highest (ng/mg Hb ± SD) 3.7 ± 0.5 detected 24 hr after OT administration at 50 mg/kg body wt. In contrast, adduct levels for pooled Alb samples increased from 0.7 ng/mg Alb at 2 hr to 2.5 ng/mg Alb at 4 hr, but were not detectable 24 hr after dosing. Hb adducts showed a linear relationship for OT doses of 10, 20, 40, 50, and 100 mg/kg body wt. The Hb adduct
t
1
2
(11 days) was determined after a single 100 mg/kg OT dose. Hb adduct levels were quantifiable (1.3 ± 0.2 ng/mg Hb) by HPLC/fluorescence 28 days after 100 mg/kg OT. Although Hb and Alb adducts differ in stability, a ratio of such OT adducts may be useful in long-term industrial biomonitoring for evaluation of OT exposure.</description><identifier>ISSN: 0272-0590</identifier><identifier>EISSN: 1095-6832</identifier><identifier>DOI: 10.1016/0272-0590(92)90111-T</identifier><identifier>PMID: 1526364</identifier><language>eng</language><publisher>United States: Elsevier Science (USA)</publisher><subject>Animals ; Chromatography, High Pressure Liquid ; Chromatography, Liquid ; Chromatography, Paper ; Chromatography, Thin Layer ; Drug Administration Routes ; Fluorescence ; Gas Chromatography-Mass Spectrometry ; Hemoglobins - isolation & purification ; Hemoglobins - metabolism ; Male ; Rats ; Rats, Inbred Strains ; Serum Albumin - isolation & purification ; Serum Albumin - metabolism ; Toluidines - blood</subject><ispartof>Fundamental and applied toxicology, 1992-05, Vol.18 (4), p.522-531</ispartof><rights>1992</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27915,27916</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1526364$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cheever, Kenneth L.</creatorcontrib><creatorcontrib>DeBord, D.Gayle</creatorcontrib><creatorcontrib>Swearengin, Terri F.</creatorcontrib><creatorcontrib>Booth-Jones, Angela D.</creatorcontrib><title>ortho-toluidine blood protein adducts: HPLC analysis with fluorescence detection after a single dose in the adult male rat</title><title>Fundamental and applied toxicology</title><addtitle>Fundam Appl Toxicol</addtitle><description>Hemoglobin (Hb) and albumin (Alb) adducts of the suspect human carcinogen
ortho-toluidine (OT) were quantified in blood samples collected from rats after a single i.p. injection. Mild alkaline hydrolysis of Hb adducted with [
14C]OT followed by extraction with ethyl acetate resulted in recovery of 63% of the bound radioactivity. HPLC analysis revealed a single radiolabeled peak which was identified as OT by GC-MS. In subsequent experiments Hb and Alb adduct levels were determined by HPLC analysis of this cleavage product using fluorescence detection. 4-Ethylaniline was used as internal standard. The detection limit for OT was approximately 450 pg/injection or 5 pmol/mg Hb. Mean adduct levels for Hb increased rapidly over the first 4 hr with the highest (ng/mg Hb ± SD) 3.7 ± 0.5 detected 24 hr after OT administration at 50 mg/kg body wt. In contrast, adduct levels for pooled Alb samples increased from 0.7 ng/mg Alb at 2 hr to 2.5 ng/mg Alb at 4 hr, but were not detectable 24 hr after dosing. Hb adducts showed a linear relationship for OT doses of 10, 20, 40, 50, and 100 mg/kg body wt. The Hb adduct
t
1
2
(11 days) was determined after a single 100 mg/kg OT dose. Hb adduct levels were quantifiable (1.3 ± 0.2 ng/mg Hb) by HPLC/fluorescence 28 days after 100 mg/kg OT. Although Hb and Alb adducts differ in stability, a ratio of such OT adducts may be useful in long-term industrial biomonitoring for evaluation of OT exposure.</description><subject>Animals</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Chromatography, Liquid</subject><subject>Chromatography, Paper</subject><subject>Chromatography, Thin Layer</subject><subject>Drug Administration Routes</subject><subject>Fluorescence</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Hemoglobins - isolation & purification</subject><subject>Hemoglobins - metabolism</subject><subject>Male</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Serum Albumin - isolation & purification</subject><subject>Serum Albumin - metabolism</subject><subject>Toluidines - blood</subject><issn>0272-0590</issn><issn>1095-6832</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9UctqHDEQFCbBXj_-wAadgn2YRI_V7CgHg1niByzEh_VZ6NHyKmhHjqRJcL7e2njxqaGruuiqQuickq-U0P4bYQvWESHJpWRXklBKu_UBmlEiRdcPnH1Csw_KETou5RdpJDEnh-iQCtbzfj5D_1Kum9TVFKfgwgjYxJQcfsmpQhixdm6ytXzH94-rJdajjq8lFPw31A32cUoZioXRAnZQwdaQ2omvkLHGJYzPsQGpAG5KdQNNbYoVb3VbZ11P0WevY4Gz_TxBT7c_1sv7bvXz7mF5s-qASVY7K7yFhRMA1vihB6Od0ZIKQvreevCDcYIstDAwcE6Y7-VcGGK49tq4gQt-gr686zZTvycoVW1D-zpGPUKaiqItCS750IgXe-JktuDUSw5bnV_VPqyGX7_j0L79EyCrYsPOvQu5mVcuBUWJ2pWjdsmrXfJKMvW_HLXmb4hFg2Y</recordid><startdate>19920501</startdate><enddate>19920501</enddate><creator>Cheever, Kenneth L.</creator><creator>DeBord, D.Gayle</creator><creator>Swearengin, Terri F.</creator><creator>Booth-Jones, Angela D.</creator><general>Elsevier Science (USA)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>19920501</creationdate><title>ortho-toluidine blood protein adducts: HPLC analysis with fluorescence detection after a single dose in the adult male rat</title><author>Cheever, Kenneth L. ; DeBord, D.Gayle ; Swearengin, Terri F. ; Booth-Jones, Angela D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e292t-c5fce7d5eecbf86ebadba9150066cfef8bd507a5be83302f6945b0b3afabd8353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Chromatography, Liquid</topic><topic>Chromatography, Paper</topic><topic>Chromatography, Thin Layer</topic><topic>Drug Administration Routes</topic><topic>Fluorescence</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Hemoglobins - isolation & purification</topic><topic>Hemoglobins - metabolism</topic><topic>Male</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Serum Albumin - isolation & purification</topic><topic>Serum Albumin - metabolism</topic><topic>Toluidines - blood</topic><toplevel>online_resources</toplevel><creatorcontrib>Cheever, Kenneth L.</creatorcontrib><creatorcontrib>DeBord, D.Gayle</creatorcontrib><creatorcontrib>Swearengin, Terri F.</creatorcontrib><creatorcontrib>Booth-Jones, Angela D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Fundamental and applied toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheever, Kenneth L.</au><au>DeBord, D.Gayle</au><au>Swearengin, Terri F.</au><au>Booth-Jones, Angela D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ortho-toluidine blood protein adducts: HPLC analysis with fluorescence detection after a single dose in the adult male rat</atitle><jtitle>Fundamental and applied toxicology</jtitle><addtitle>Fundam Appl Toxicol</addtitle><date>1992-05-01</date><risdate>1992</risdate><volume>18</volume><issue>4</issue><spage>522</spage><epage>531</epage><pages>522-531</pages><issn>0272-0590</issn><eissn>1095-6832</eissn><abstract>Hemoglobin (Hb) and albumin (Alb) adducts of the suspect human carcinogen
ortho-toluidine (OT) were quantified in blood samples collected from rats after a single i.p. injection. Mild alkaline hydrolysis of Hb adducted with [
14C]OT followed by extraction with ethyl acetate resulted in recovery of 63% of the bound radioactivity. HPLC analysis revealed a single radiolabeled peak which was identified as OT by GC-MS. In subsequent experiments Hb and Alb adduct levels were determined by HPLC analysis of this cleavage product using fluorescence detection. 4-Ethylaniline was used as internal standard. The detection limit for OT was approximately 450 pg/injection or 5 pmol/mg Hb. Mean adduct levels for Hb increased rapidly over the first 4 hr with the highest (ng/mg Hb ± SD) 3.7 ± 0.5 detected 24 hr after OT administration at 50 mg/kg body wt. In contrast, adduct levels for pooled Alb samples increased from 0.7 ng/mg Alb at 2 hr to 2.5 ng/mg Alb at 4 hr, but were not detectable 24 hr after dosing. Hb adducts showed a linear relationship for OT doses of 10, 20, 40, 50, and 100 mg/kg body wt. The Hb adduct
t
1
2
(11 days) was determined after a single 100 mg/kg OT dose. Hb adduct levels were quantifiable (1.3 ± 0.2 ng/mg Hb) by HPLC/fluorescence 28 days after 100 mg/kg OT. Although Hb and Alb adducts differ in stability, a ratio of such OT adducts may be useful in long-term industrial biomonitoring for evaluation of OT exposure.</abstract><cop>United States</cop><pub>Elsevier Science (USA)</pub><pmid>1526364</pmid><doi>10.1016/0272-0590(92)90111-T</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0272-0590 |
| ispartof | Fundamental and applied toxicology, 1992-05, Vol.18 (4), p.522-531 |
| issn | 0272-0590 1095-6832 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16363938 |
| source | MEDLINE; Oxford Journals A-Z Collection; Alma/SFX Local Collection |
| subjects | Animals Chromatography, High Pressure Liquid Chromatography, Liquid Chromatography, Paper Chromatography, Thin Layer Drug Administration Routes Fluorescence Gas Chromatography-Mass Spectrometry Hemoglobins - isolation & purification Hemoglobins - metabolism Male Rats Rats, Inbred Strains Serum Albumin - isolation & purification Serum Albumin - metabolism Toluidines - blood |
| title | ortho-toluidine blood protein adducts: HPLC analysis with fluorescence detection after a single dose in the adult male rat |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T17%3A52%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=ortho-toluidine%20blood%20protein%20adducts:%20HPLC%20analysis%20with%20fluorescence%20detection%20after%20a%20single%20dose%20in%20the%20adult%20male%20rat&rft.jtitle=Fundamental%20and%20applied%20toxicology&rft.au=Cheever,%20Kenneth%20L.&rft.date=1992-05-01&rft.volume=18&rft.issue=4&rft.spage=522&rft.epage=531&rft.pages=522-531&rft.issn=0272-0590&rft.eissn=1095-6832&rft_id=info:doi/10.1016/0272-0590(92)90111-T&rft_dat=%3Cproquest_pubme%3E16363938%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16363938&rft_id=info:pmid/1526364&rft_els_id=027205909290111T&rfr_iscdi=true |