Molecular cloning and characterization of the major endothelin receptor subtype in porcine cerebellum

Molecular cloning and characterization of the major endothelin receptor subtype in porcine cerebellum

Endothelin receptors (ETRs) display subtype heterogeneity and are widely distributed throughout the tissues of the periphery and central nervous system. In order to gain further insight into the potential molecular differences of ETRs, we initiated molecular cloning of ETR genes by screening for the...

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Veröffentlicht in:Molecular pharmacology 1992-01, Vol.41 (2), p.465-473
Hauptverfasser: Elshourbagy, NA, Lee, JA, Korman, DR, Nuthalaganti, P, Sylvester, DR, Dilella, A G, Sutiphong, JA, Kumar, C S
Format: Artikel
Sprache:eng
Schlagworte:
amino acid sequence
cDNA
cerebellum
characterization
endothelin
genes
isoforms
membrane proteins
nucleotide sequence
pigs
prediction
receptors
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Zusammenfassung:Endothelin receptors (ETRs) display subtype heterogeneity and are widely distributed throughout the tissues of the periphery and central nervous system. In order to gain further insight into the potential molecular differences of ETRs, we initiated molecular cloning of ETR genes by screening for the appearance of super(125)I-ET-1 binding activity in COS cells transfected with pools of a porcine cerebellum cDNA expression library. The data demonstrate that the pPCETR 1.1 encodes the major ETR subtype in the porcine cerebellum.
ISSN:0026-895X