Development and Evaluation of an ELISA to Measure Antibody Responses to Both the Nucleocapsid and Spike Proteins of Canine Coronavirus
A rapid and reproducible enzyme linked immunosorbent assay (ELISA) was developed for detection of canine coronavirus (CCV) specific antibodies directed to both the nucleocapsid (NC) and the spike (S) proteins. The coating antigen, a methanol-treated, S-protein enriched preparation, was produced by s...
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| Veröffentlicht in: | Journal of immunoassay (Monticello, N.Y.) N.Y.), 1998-02, Vol.19 (1), p.1-22 |
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| container_title | Journal of immunoassay (Monticello, N.Y.) |
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| creator | Palmer-Densmore, Melissa L. Johnson, Anthony F. Sabara, Marta I. J. |
| description | A rapid and reproducible enzyme linked immunosorbent assay (ELISA) was developed for detection of canine coronavirus (CCV) specific antibodies directed to both the nucleocapsid (NC) and the spike (S) proteins. The coating antigen, a methanol-treated, S-protein enriched preparation, was produced by subjecting infected cells to Triton X-114 detergent followed by phase separation. The sensitivity of this assay was determined by following the course of infection in dogs experimentally infected with CCV. The specificity of the antibody response was determined by Western blot analysis and supported the increased magnitude of the ELISA response and the presence of serum neutralizing (SN) antibody. Due to the sensitivity and specificity of the IgG response detected by this assay it can be used to determine both virus exposure and vaccine efficacy. |
| doi_str_mv | 10.1080/01971529808005468 |
| format | Article |
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Due to the sensitivity and specificity of the IgG response detected by this assay it can be used to determine both virus exposure and vaccine efficacy.</description><subject>Animals</subject><subject>Antibodies, Viral - immunology</subject><subject>Antibody Formation</subject><subject>Antibody Specificity</subject><subject>Antigens, Viral - immunology</subject><subject>Blotting, Western</subject><subject>CCV</subject><subject>Coating antigen preparation</subject><subject>Coronavirus Infections - immunology</subject><subject>Coronavirus, Canine - immunology</subject><subject>Dogs</subject><subject>Enzyme immunoassay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Evaluation Studies as Topic</subject><subject>Immunoglobulin G - analysis</subject><subject>Methanol</subject><subject>Neutralization Tests</subject><subject>Nucleocapsid Proteins - immunology</subject><subject>Sensitivity and Specificity</subject><subject>Time Factors</subject><subject>Triton X-114</subject><subject>Viral Proteins - immunology</subject><issn>0197-1522</issn><issn>2332-4090</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS0EKqPSB2CB5BW7UP_FdiQ2w3QolYYfUVhHjnOjGhI72M7AvADPTcKM2FSod2Ndn3M-Xekg9JySV5RocklopWjJKj0vpBRSP0IrxjkrBKnIY7Ra9GI2sKfoIqVvZJ5KEKHlGTqrSk4k0Sv0-wr20IdxAJ-x8S3e7k0_meyCx6Gbf_B2d3O7xjng92DSFAGvfXZNaA_4M6Qx-ARpUd-EfIfzHeAPk-0hWDMm1_4l3o7uO-BPMWRwPi3UjfHOA96EGLzZuzilZ-hJZ_oEF6f3HH19u_2yeVfsPl7fbNa7wgqmc6FaCS0TjHSqaRhvtaKaa2AV5dwyqUVjmLSaEWY5YWVpAEqjpFQgOgEN5-fo5ZE7xvBjgpTrwSULfW88hCnVqlKyoqJ80EglL4kQC5EejTaGlCJ09RjdYOKhpqReeqrv9TRnXpzgUzNA-y9xamXW1VF3vgtxMD9D7Ns6m0MfYheNty7dp9b5V56Trx9M8v8f9gfejLJy</recordid><startdate>199802</startdate><enddate>199802</enddate><creator>Palmer-Densmore, Melissa L.</creator><creator>Johnson, Anthony F.</creator><creator>Sabara, Marta I. 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J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and Evaluation of an ELISA to Measure Antibody Responses to Both the Nucleocapsid and Spike Proteins of Canine Coronavirus</atitle><jtitle>Journal of immunoassay (Monticello, N.Y.)</jtitle><addtitle>J Immunoassay</addtitle><date>1998-02</date><risdate>1998</risdate><volume>19</volume><issue>1</issue><spage>1</spage><epage>22</epage><pages>1-22</pages><issn>0197-1522</issn><eissn>2332-4090</eissn><abstract>A rapid and reproducible enzyme linked immunosorbent assay (ELISA) was developed for detection of canine coronavirus (CCV) specific antibodies directed to both the nucleocapsid (NC) and the spike (S) proteins. The coating antigen, a methanol-treated, S-protein enriched preparation, was produced by subjecting infected cells to Triton X-114 detergent followed by phase separation. The sensitivity of this assay was determined by following the course of infection in dogs experimentally infected with CCV. The specificity of the antibody response was determined by Western blot analysis and supported the increased magnitude of the ELISA response and the presence of serum neutralizing (SN) antibody. Due to the sensitivity and specificity of the IgG response detected by this assay it can be used to determine both virus exposure and vaccine efficacy.</abstract><cop>United States</cop><pub>Taylor & Francis Group</pub><pmid>9530608</pmid><doi>10.1080/01971529808005468</doi><tpages>22</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0197-1522 |
| ispartof | Journal of immunoassay (Monticello, N.Y.), 1998-02, Vol.19 (1), p.1-22 |
| issn | 0197-1522 2332-4090 |
| language | eng |
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| source | Taylor & Francis Online; MEDLINE |
| subjects | Animals Antibodies, Viral - immunology Antibody Formation Antibody Specificity Antigens, Viral - immunology Blotting, Western CCV Coating antigen preparation Coronavirus Infections - immunology Coronavirus, Canine - immunology Dogs Enzyme immunoassay Enzyme-Linked Immunosorbent Assay - methods Evaluation Studies as Topic Immunoglobulin G - analysis Methanol Neutralization Tests Nucleocapsid Proteins - immunology Sensitivity and Specificity Time Factors Triton X-114 Viral Proteins - immunology |
| title | Development and Evaluation of an ELISA to Measure Antibody Responses to Both the Nucleocapsid and Spike Proteins of Canine Coronavirus |
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