Expression of phosphorylated cyclic AMP response element-binding protein in melanin-concentrating hormone neurons and orexin neurons in male and female rats during ad-libitum feeding

Using phosphorylated cyclic AMP response element-binding protein (pCREB) as a marker of neural activity, we previously suggested that orexin neurons and melanin-concentrating hormone (MCH) neurons play distinct roles in feeding behavior. In the present study, we examined the expression of pCREB duri...

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Veröffentlicht in:Neuroreport 2014-07, Vol.25 (10), p.766-770
Hauptverfasser: Fukushima, Atsushi, Hagiwara, Hiroko, Yoshioka, Nozomu, Kimura, Fukuko, Akema, Tatsuo, Funabashi, Toshiya
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container_issue 10
container_start_page 766
container_title Neuroreport
container_volume 25
creator Fukushima, Atsushi
Hagiwara, Hiroko
Yoshioka, Nozomu
Kimura, Fukuko
Akema, Tatsuo
Funabashi, Toshiya
description Using phosphorylated cyclic AMP response element-binding protein (pCREB) as a marker of neural activity, we previously suggested that orexin neurons and melanin-concentrating hormone (MCH) neurons play distinct roles in feeding behavior. In the present study, we examined the expression of pCREB during ad-libitum feeding; previously, only fasted animals were examined. MCH neurons, but not orexin neurons, expressed pCREB during spontaneous food intake. The induction of pCREB expression did not differ by sex, but attenuation seemed to occur faster in females than in males. On the basis of the results of the present study, we speculate that MCH neurons respond to nutrition-related feeding, but the feeding-related activity of orexin was not evident unless hunger was accompanied by stress, such as the stress caused by the absence of food in the case of fasting. Therefore, the desire to eat under normal conditions does not drive orexin neurons, but it does drive MCH neurons. We tested this hypothesis by examining the effects of consuming glucose or saccharin, a nonmetabolized sweetener, in fasted male and female rats. Glucose and saccharin were equally effective in reducing pCREB expression in the orexin neurons of female rats. In MCH neurons, glucose attenuated the expression of pCREB, but saccharin had no effect, irrespective of sex. Taken together, the results indicate that MCH and orexin peptides play physiologically distinct roles in feeding behavior.
doi_str_mv 10.1097/WNR.0000000000000172
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subjects Animals
Biological and medical sciences
Cyclic AMP Response Element-Binding Protein - metabolism
Feeding Behavior - physiology
Female
Fundamental and applied biological sciences. Psychology
Hypothalamic Hormones - metabolism
Intracellular Signaling Peptides and Proteins - metabolism
Male
Melanins - metabolism
Neurons - metabolism
Neuropeptides - metabolism
Orexins
Phosphorylation
Pituitary Hormones - metabolism
Rats
Rats, Wistar
Sex Factors
Vertebrates: nervous system and sense organs
title Expression of phosphorylated cyclic AMP response element-binding protein in melanin-concentrating hormone neurons and orexin neurons in male and female rats during ad-libitum feeding
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