Expression of phosphorylated cyclic AMP response element-binding protein in melanin-concentrating hormone neurons and orexin neurons in male and female rats during ad-libitum feeding
Using phosphorylated cyclic AMP response element-binding protein (pCREB) as a marker of neural activity, we previously suggested that orexin neurons and melanin-concentrating hormone (MCH) neurons play distinct roles in feeding behavior. In the present study, we examined the expression of pCREB duri...
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Veröffentlicht in: | Neuroreport 2014-07, Vol.25 (10), p.766-770 |
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description | Using phosphorylated cyclic AMP response element-binding protein (pCREB) as a marker of neural activity, we previously suggested that orexin neurons and melanin-concentrating hormone (MCH) neurons play distinct roles in feeding behavior. In the present study, we examined the expression of pCREB during ad-libitum feeding; previously, only fasted animals were examined. MCH neurons, but not orexin neurons, expressed pCREB during spontaneous food intake. The induction of pCREB expression did not differ by sex, but attenuation seemed to occur faster in females than in males. On the basis of the results of the present study, we speculate that MCH neurons respond to nutrition-related feeding, but the feeding-related activity of orexin was not evident unless hunger was accompanied by stress, such as the stress caused by the absence of food in the case of fasting. Therefore, the desire to eat under normal conditions does not drive orexin neurons, but it does drive MCH neurons. We tested this hypothesis by examining the effects of consuming glucose or saccharin, a nonmetabolized sweetener, in fasted male and female rats. Glucose and saccharin were equally effective in reducing pCREB expression in the orexin neurons of female rats. In MCH neurons, glucose attenuated the expression of pCREB, but saccharin had no effect, irrespective of sex. Taken together, the results indicate that MCH and orexin peptides play physiologically distinct roles in feeding behavior. |
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In the present study, we examined the expression of pCREB during ad-libitum feeding; previously, only fasted animals were examined. MCH neurons, but not orexin neurons, expressed pCREB during spontaneous food intake. The induction of pCREB expression did not differ by sex, but attenuation seemed to occur faster in females than in males. On the basis of the results of the present study, we speculate that MCH neurons respond to nutrition-related feeding, but the feeding-related activity of orexin was not evident unless hunger was accompanied by stress, such as the stress caused by the absence of food in the case of fasting. Therefore, the desire to eat under normal conditions does not drive orexin neurons, but it does drive MCH neurons. We tested this hypothesis by examining the effects of consuming glucose or saccharin, a nonmetabolized sweetener, in fasted male and female rats. Glucose and saccharin were equally effective in reducing pCREB expression in the orexin neurons of female rats. In MCH neurons, glucose attenuated the expression of pCREB, but saccharin had no effect, irrespective of sex. Taken together, the results indicate that MCH and orexin peptides play physiologically distinct roles in feeding behavior.</description><identifier>ISSN: 0959-4965</identifier><identifier>EISSN: 1473-558X</identifier><identifier>DOI: 10.1097/WNR.0000000000000172</identifier><identifier>PMID: 24780894</identifier><language>eng</language><publisher>Hagerstown, MD: Wolters Kluwer Health | Lippincott Williams & Wilkins</publisher><subject>Animals ; Biological and medical sciences ; Cyclic AMP Response Element-Binding Protein - metabolism ; Feeding Behavior - physiology ; Female ; Fundamental and applied biological sciences. Psychology ; Hypothalamic Hormones - metabolism ; Intracellular Signaling Peptides and Proteins - metabolism ; Male ; Melanins - metabolism ; Neurons - metabolism ; Neuropeptides - metabolism ; Orexins ; Phosphorylation ; Pituitary Hormones - metabolism ; Rats ; Rats, Wistar ; Sex Factors ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroreport, 2014-07, Vol.25 (10), p.766-770</ispartof><rights>2014 Wolters Kluwer Health | Lippincott Williams & Wilkins</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4852-3444d6f572cbebce3b19ba51ae66eadbdddbfcb9e4dbfb1ce817e42f0aed87f13</citedby><cites>FETCH-LOGICAL-c4852-3444d6f572cbebce3b19ba51ae66eadbdddbfcb9e4dbfb1ce817e42f0aed87f13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28583628$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24780894$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fukushima, Atsushi</creatorcontrib><creatorcontrib>Hagiwara, Hiroko</creatorcontrib><creatorcontrib>Yoshioka, Nozomu</creatorcontrib><creatorcontrib>Kimura, Fukuko</creatorcontrib><creatorcontrib>Akema, Tatsuo</creatorcontrib><creatorcontrib>Funabashi, Toshiya</creatorcontrib><title>Expression of phosphorylated cyclic AMP response element-binding protein in melanin-concentrating hormone neurons and orexin neurons in male and female rats during ad-libitum feeding</title><title>Neuroreport</title><addtitle>Neuroreport</addtitle><description>Using phosphorylated cyclic AMP response element-binding protein (pCREB) as a marker of neural activity, we previously suggested that orexin neurons and melanin-concentrating hormone (MCH) neurons play distinct roles in feeding behavior. In the present study, we examined the expression of pCREB during ad-libitum feeding; previously, only fasted animals were examined. MCH neurons, but not orexin neurons, expressed pCREB during spontaneous food intake. The induction of pCREB expression did not differ by sex, but attenuation seemed to occur faster in females than in males. On the basis of the results of the present study, we speculate that MCH neurons respond to nutrition-related feeding, but the feeding-related activity of orexin was not evident unless hunger was accompanied by stress, such as the stress caused by the absence of food in the case of fasting. Therefore, the desire to eat under normal conditions does not drive orexin neurons, but it does drive MCH neurons. We tested this hypothesis by examining the effects of consuming glucose or saccharin, a nonmetabolized sweetener, in fasted male and female rats. Glucose and saccharin were equally effective in reducing pCREB expression in the orexin neurons of female rats. In MCH neurons, glucose attenuated the expression of pCREB, but saccharin had no effect, irrespective of sex. Taken together, the results indicate that MCH and orexin peptides play physiologically distinct roles in feeding behavior.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cyclic AMP Response Element-Binding Protein - metabolism</subject><subject>Feeding Behavior - physiology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hypothalamic Hormones - metabolism</subject><subject>Intracellular Signaling Peptides and Proteins - metabolism</subject><subject>Male</subject><subject>Melanins - metabolism</subject><subject>Neurons - metabolism</subject><subject>Neuropeptides - metabolism</subject><subject>Orexins</subject><subject>Phosphorylation</subject><subject>Pituitary Hormones - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Sex Factors</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0959-4965</issn><issn>1473-558X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1TAQhi0EoofCGyDkDRKbFDuxE2dZVeUilYsQCHaRL2OOwbFTO1F7Xoznw-k5BcQCLFtjeb7_H48GoceUnFDSd88_v_1wQv5ctKvvoA1lXVNxLr7cRRvS875ifcuP0IOcvxWmJ1TcR0c16wQRPdugH-fXU4KcXQw4WjxtYy4n7bycwWC9095pfPrmPS7QFEMGDB5GCHOlXDAufMVTijO4gMsewcvgQqVj0AVJcl6BYjfGADjAkooDlsHgmOC6CG6fVq30cJOycHMt4ozNklYHaSrvlJuXsWRhrfoQ3bPSZ3h0iMfo04vzj2evqot3L1-fnV5UmgleVw1jzLSWd7VWoDQ0ivZKciqhbUEaZYxRVqseWImKahC0A1ZbIsGIztLmGD3b-5YuLxfI8zC6rMGXPiEueaBtwwkjraj_j_KGt3XbEVZQtkd1ijknsMOU3CjTbqBkWIc7lOEOfw-3yJ4cKixqBPNLdDvNAjw9ADJr6W2SQbv8mxNcNG0tCif23FX0M6T83S9XkIYtSD9v__2Hn9COxE0</recordid><startdate>20140709</startdate><enddate>20140709</enddate><creator>Fukushima, Atsushi</creator><creator>Hagiwara, Hiroko</creator><creator>Yoshioka, Nozomu</creator><creator>Kimura, Fukuko</creator><creator>Akema, Tatsuo</creator><creator>Funabashi, Toshiya</creator><general>Wolters Kluwer Health | Lippincott Williams & Wilkins</general><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope></search><sort><creationdate>20140709</creationdate><title>Expression of phosphorylated cyclic AMP response element-binding protein in melanin-concentrating hormone neurons and orexin neurons in male and female rats during ad-libitum feeding</title><author>Fukushima, Atsushi ; Hagiwara, Hiroko ; Yoshioka, Nozomu ; Kimura, Fukuko ; Akema, Tatsuo ; Funabashi, Toshiya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4852-3444d6f572cbebce3b19ba51ae66eadbdddbfcb9e4dbfb1ce817e42f0aed87f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cyclic AMP Response Element-Binding Protein - metabolism</topic><topic>Feeding Behavior - physiology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hypothalamic Hormones - metabolism</topic><topic>Intracellular Signaling Peptides and Proteins - metabolism</topic><topic>Male</topic><topic>Melanins - metabolism</topic><topic>Neurons - metabolism</topic><topic>Neuropeptides - metabolism</topic><topic>Orexins</topic><topic>Phosphorylation</topic><topic>Pituitary Hormones - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Sex Factors</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fukushima, Atsushi</creatorcontrib><creatorcontrib>Hagiwara, Hiroko</creatorcontrib><creatorcontrib>Yoshioka, Nozomu</creatorcontrib><creatorcontrib>Kimura, Fukuko</creatorcontrib><creatorcontrib>Akema, Tatsuo</creatorcontrib><creatorcontrib>Funabashi, Toshiya</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><jtitle>Neuroreport</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fukushima, Atsushi</au><au>Hagiwara, Hiroko</au><au>Yoshioka, Nozomu</au><au>Kimura, Fukuko</au><au>Akema, Tatsuo</au><au>Funabashi, Toshiya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of phosphorylated cyclic AMP response element-binding protein in melanin-concentrating hormone neurons and orexin neurons in male and female rats during ad-libitum feeding</atitle><jtitle>Neuroreport</jtitle><addtitle>Neuroreport</addtitle><date>2014-07-09</date><risdate>2014</risdate><volume>25</volume><issue>10</issue><spage>766</spage><epage>770</epage><pages>766-770</pages><issn>0959-4965</issn><eissn>1473-558X</eissn><abstract>Using phosphorylated cyclic AMP response element-binding protein (pCREB) as a marker of neural activity, we previously suggested that orexin neurons and melanin-concentrating hormone (MCH) neurons play distinct roles in feeding behavior. In the present study, we examined the expression of pCREB during ad-libitum feeding; previously, only fasted animals were examined. MCH neurons, but not orexin neurons, expressed pCREB during spontaneous food intake. The induction of pCREB expression did not differ by sex, but attenuation seemed to occur faster in females than in males. On the basis of the results of the present study, we speculate that MCH neurons respond to nutrition-related feeding, but the feeding-related activity of orexin was not evident unless hunger was accompanied by stress, such as the stress caused by the absence of food in the case of fasting. Therefore, the desire to eat under normal conditions does not drive orexin neurons, but it does drive MCH neurons. We tested this hypothesis by examining the effects of consuming glucose or saccharin, a nonmetabolized sweetener, in fasted male and female rats. Glucose and saccharin were equally effective in reducing pCREB expression in the orexin neurons of female rats. In MCH neurons, glucose attenuated the expression of pCREB, but saccharin had no effect, irrespective of sex. Taken together, the results indicate that MCH and orexin peptides play physiologically distinct roles in feeding behavior.</abstract><cop>Hagerstown, MD</cop><pub>Wolters Kluwer Health | Lippincott Williams & Wilkins</pub><pmid>24780894</pmid><doi>10.1097/WNR.0000000000000172</doi><tpages>5</tpages></addata></record> |
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subjects | Animals Biological and medical sciences Cyclic AMP Response Element-Binding Protein - metabolism Feeding Behavior - physiology Female Fundamental and applied biological sciences. Psychology Hypothalamic Hormones - metabolism Intracellular Signaling Peptides and Proteins - metabolism Male Melanins - metabolism Neurons - metabolism Neuropeptides - metabolism Orexins Phosphorylation Pituitary Hormones - metabolism Rats Rats, Wistar Sex Factors Vertebrates: nervous system and sense organs |
title | Expression of phosphorylated cyclic AMP response element-binding protein in melanin-concentrating hormone neurons and orexin neurons in male and female rats during ad-libitum feeding |
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