Propionibacterium acnes Activates the NLRP3 Inflammasome in Human Sebocytes
Propionibacterium acne and sebaceous glands are considered to have an important role in the development of acne. Although information regarding the activation of innate immunity by P. acnes in the sebaceous gland is limited, different P. acnes phylotypes and a higher prevalence of follicular P. acne...
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| Veröffentlicht in: | Journal of investigative dermatology 2014-11, Vol.134 (11), p.2747-2756 |
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| creator | Li, Zheng Jun Choi, Dae Kyoung Sohn, Kyung Cheol Seo, Min Seok Lee, Hae Eul Lee, Young Seo, Young Joon Lee, Young Ho Shi, Ge Zouboulis, Christos C. Kim, Chang Deok Lee, Jeung Hoon Im, Myung |
| description | Propionibacterium acne and sebaceous glands are considered to have an important role in the development of acne. Although information regarding the activation of innate immunity by P. acnes in the sebaceous gland is limited, different P. acnes phylotypes and a higher prevalence of follicular P. acnes macrocolonies/biofilms in sebaceous follicles of skin biopsies from acne compared with control skin and occasionally single P. acnes clusters in single sebaceous glands have been detected. In this study, we investigated whether P. acnes activates the inflammasome in human sebaceous glands in vivo and in vitro. We found that IL-1β expression was upregulated in sebaceous glands of acne lesions. After stimulation of human sebocytes with P. acnes, the activation of caspase-1 and secretion of IL-1β were enhanced significantly. Moreover, knocking down the expression of NLRP3 abolished P. acnes-induced IL-1β production in sebocytes. The activation of the NLRP3 inflammasome by P. acnes was dependent on protease activity and reactive oxygen species generation. Finally, we found that NALP3-deficient mice display an impaired inflammatory response to P. acnes. These results suggest that human sebocytes are important immunocompetent cells that induce the NLRP3 inflammasome, and that P. acnes-induced IL-1β activation in sebaceous glands may have a role in combating skin infections and in acne pathogenesis. |
| doi_str_mv | 10.1038/jid.2014.221 |
| format | Article |
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Although information regarding the activation of innate immunity by P. acnes in the sebaceous gland is limited, different P. acnes phylotypes and a higher prevalence of follicular P. acnes macrocolonies/biofilms in sebaceous follicles of skin biopsies from acne compared with control skin and occasionally single P. acnes clusters in single sebaceous glands have been detected. In this study, we investigated whether P. acnes activates the inflammasome in human sebaceous glands in vivo and in vitro. We found that IL-1β expression was upregulated in sebaceous glands of acne lesions. After stimulation of human sebocytes with P. acnes, the activation of caspase-1 and secretion of IL-1β were enhanced significantly. Moreover, knocking down the expression of NLRP3 abolished P. acnes-induced IL-1β production in sebocytes. The activation of the NLRP3 inflammasome by P. acnes was dependent on protease activity and reactive oxygen species generation. Finally, we found that NALP3-deficient mice display an impaired inflammatory response to P. acnes. These results suggest that human sebocytes are important immunocompetent cells that induce the NLRP3 inflammasome, and that P. acnes-induced IL-1β activation in sebaceous glands may have a role in combating skin infections and in acne pathogenesis.</description><identifier>ISSN: 0022-202X</identifier><identifier>EISSN: 1523-1747</identifier><identifier>DOI: 10.1038/jid.2014.221</identifier><identifier>PMID: 24820890</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acne Vulgaris - immunology ; Acne Vulgaris - microbiology ; Adult ; Animals ; Carrier Proteins - metabolism ; Caspase 1 - metabolism ; Cells, Cultured ; Female ; Humans ; Inflammation ; Interleukin-1beta - metabolism ; Male ; Mice ; Mice, Inbred C57BL ; NLR Family, Pyrin Domain-Containing 3 Protein ; Peptide Hydrolases - metabolism ; Propionibacterium acnes ; Propionibacterium acnes - metabolism ; Reactive Oxygen Species ; Sebaceous Glands - cytology ; Sebaceous Glands - microbiology ; Skin - metabolism ; Skin - microbiology ; Up-Regulation ; Young Adult</subject><ispartof>Journal of investigative dermatology, 2014-11, Vol.134 (11), p.2747-2756</ispartof><rights>2014 The Society for Investigative Dermatology, Inc</rights><rights>Copyright Nature Publishing Group Nov 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c573t-b33b934b2d5e7db7a5f941111d950e87faa45e375396e21fd2ed7007fadc35d13</citedby><cites>FETCH-LOGICAL-c573t-b33b934b2d5e7db7a5f941111d950e87faa45e375396e21fd2ed7007fadc35d13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1612293897?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24820890$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Zheng Jun</creatorcontrib><creatorcontrib>Choi, Dae Kyoung</creatorcontrib><creatorcontrib>Sohn, Kyung Cheol</creatorcontrib><creatorcontrib>Seo, Min Seok</creatorcontrib><creatorcontrib>Lee, Hae Eul</creatorcontrib><creatorcontrib>Lee, Young</creatorcontrib><creatorcontrib>Seo, Young Joon</creatorcontrib><creatorcontrib>Lee, Young Ho</creatorcontrib><creatorcontrib>Shi, Ge</creatorcontrib><creatorcontrib>Zouboulis, Christos C.</creatorcontrib><creatorcontrib>Kim, Chang Deok</creatorcontrib><creatorcontrib>Lee, Jeung Hoon</creatorcontrib><creatorcontrib>Im, Myung</creatorcontrib><title>Propionibacterium acnes Activates the NLRP3 Inflammasome in Human Sebocytes</title><title>Journal of investigative dermatology</title><addtitle>J Invest Dermatol</addtitle><description>Propionibacterium acne and sebaceous glands are considered to have an important role in the development of acne. Although information regarding the activation of innate immunity by P. acnes in the sebaceous gland is limited, different P. acnes phylotypes and a higher prevalence of follicular P. acnes macrocolonies/biofilms in sebaceous follicles of skin biopsies from acne compared with control skin and occasionally single P. acnes clusters in single sebaceous glands have been detected. In this study, we investigated whether P. acnes activates the inflammasome in human sebaceous glands in vivo and in vitro. We found that IL-1β expression was upregulated in sebaceous glands of acne lesions. After stimulation of human sebocytes with P. acnes, the activation of caspase-1 and secretion of IL-1β were enhanced significantly. Moreover, knocking down the expression of NLRP3 abolished P. acnes-induced IL-1β production in sebocytes. The activation of the NLRP3 inflammasome by P. acnes was dependent on protease activity and reactive oxygen species generation. Finally, we found that NALP3-deficient mice display an impaired inflammatory response to P. acnes. These results suggest that human sebocytes are important immunocompetent cells that induce the NLRP3 inflammasome, and that P. acnes-induced IL-1β activation in sebaceous glands may have a role in combating skin infections and in acne pathogenesis.</description><subject>Acne Vulgaris - immunology</subject><subject>Acne Vulgaris - microbiology</subject><subject>Adult</subject><subject>Animals</subject><subject>Carrier Proteins - metabolism</subject><subject>Caspase 1 - metabolism</subject><subject>Cells, Cultured</subject><subject>Female</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Interleukin-1beta - metabolism</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>NLR Family, Pyrin Domain-Containing 3 Protein</subject><subject>Peptide Hydrolases - metabolism</subject><subject>Propionibacterium acnes</subject><subject>Propionibacterium acnes - metabolism</subject><subject>Reactive Oxygen Species</subject><subject>Sebaceous Glands - cytology</subject><subject>Sebaceous Glands - microbiology</subject><subject>Skin - metabolism</subject><subject>Skin - microbiology</subject><subject>Up-Regulation</subject><subject>Young Adult</subject><issn>0022-202X</issn><issn>1523-1747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqN0c1LwzAYBvAgipsfN89S8OLBznw0TXscQ504dPgB3kKavMWMtZlJK_jfm7HpQTyYS0L48cD7PgidEDwimBWXC2tGFJNsRCnZQUPCKUuJyMQuGmJMaUoxfR2ggxAWGJM848U-GtCsoLgo8RDdzb1bWdfaSukOvO2bROkWQjLWnf1QXXx1b5Dczx7nLLlt66VqGhVcA4ltk2nfqDZ5gsrpzyiP0F6tlgGOt_cherm-ep5M09nDze1kPEs1F6xLK8aqkmUVNRyEqYTidZmReEzJMRSiVirjwARnZQ6U1IaCERjHf6MZN4QdovNN7sq79x5CJxsbNCyXqgXXB0lyxjHDXOT_oISVnOZinXr2iy5c79s4yFpRWrKiFFFdbJT2LgQPtVx52yj_KQmW6z5k7EOu-5Cxj8hPt6F91YD5wd8FRJBvAMSFfVjwMmgLrQZjPehOGmf_Tv4CQ_uWcw</recordid><startdate>20141101</startdate><enddate>20141101</enddate><creator>Li, Zheng Jun</creator><creator>Choi, Dae Kyoung</creator><creator>Sohn, Kyung Cheol</creator><creator>Seo, Min Seok</creator><creator>Lee, Hae Eul</creator><creator>Lee, Young</creator><creator>Seo, Young Joon</creator><creator>Lee, Young Ho</creator><creator>Shi, Ge</creator><creator>Zouboulis, Christos C.</creator><creator>Kim, Chang Deok</creator><creator>Lee, Jeung Hoon</creator><creator>Im, Myung</creator><general>Elsevier Inc</general><general>Elsevier Limited</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20141101</creationdate><title>Propionibacterium acnes Activates the NLRP3 Inflammasome in Human Sebocytes</title><author>Li, Zheng Jun ; Choi, Dae Kyoung ; Sohn, Kyung Cheol ; Seo, Min Seok ; Lee, Hae Eul ; Lee, Young ; Seo, Young Joon ; Lee, Young Ho ; Shi, Ge ; Zouboulis, Christos C. ; Kim, Chang Deok ; Lee, Jeung Hoon ; Im, Myung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c573t-b33b934b2d5e7db7a5f941111d950e87faa45e375396e21fd2ed7007fadc35d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Acne Vulgaris - 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Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Journal of investigative dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Zheng Jun</au><au>Choi, Dae Kyoung</au><au>Sohn, Kyung Cheol</au><au>Seo, Min Seok</au><au>Lee, Hae Eul</au><au>Lee, Young</au><au>Seo, Young Joon</au><au>Lee, Young Ho</au><au>Shi, Ge</au><au>Zouboulis, Christos C.</au><au>Kim, Chang Deok</au><au>Lee, Jeung Hoon</au><au>Im, Myung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Propionibacterium acnes Activates the NLRP3 Inflammasome in Human Sebocytes</atitle><jtitle>Journal of investigative dermatology</jtitle><addtitle>J Invest Dermatol</addtitle><date>2014-11-01</date><risdate>2014</risdate><volume>134</volume><issue>11</issue><spage>2747</spage><epage>2756</epage><pages>2747-2756</pages><issn>0022-202X</issn><eissn>1523-1747</eissn><abstract>Propionibacterium acne and sebaceous glands are considered to have an important role in the development of acne. Although information regarding the activation of innate immunity by P. acnes in the sebaceous gland is limited, different P. acnes phylotypes and a higher prevalence of follicular P. acnes macrocolonies/biofilms in sebaceous follicles of skin biopsies from acne compared with control skin and occasionally single P. acnes clusters in single sebaceous glands have been detected. In this study, we investigated whether P. acnes activates the inflammasome in human sebaceous glands in vivo and in vitro. We found that IL-1β expression was upregulated in sebaceous glands of acne lesions. After stimulation of human sebocytes with P. acnes, the activation of caspase-1 and secretion of IL-1β were enhanced significantly. Moreover, knocking down the expression of NLRP3 abolished P. acnes-induced IL-1β production in sebocytes. The activation of the NLRP3 inflammasome by P. acnes was dependent on protease activity and reactive oxygen species generation. Finally, we found that NALP3-deficient mice display an impaired inflammatory response to P. acnes. These results suggest that human sebocytes are important immunocompetent cells that induce the NLRP3 inflammasome, and that P. acnes-induced IL-1β activation in sebaceous glands may have a role in combating skin infections and in acne pathogenesis.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>24820890</pmid><doi>10.1038/jid.2014.221</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acne Vulgaris - immunology Acne Vulgaris - microbiology Adult Animals Carrier Proteins - metabolism Caspase 1 - metabolism Cells, Cultured Female Humans Inflammation Interleukin-1beta - metabolism Male Mice Mice, Inbred C57BL NLR Family, Pyrin Domain-Containing 3 Protein Peptide Hydrolases - metabolism Propionibacterium acnes Propionibacterium acnes - metabolism Reactive Oxygen Species Sebaceous Glands - cytology Sebaceous Glands - microbiology Skin - metabolism Skin - microbiology Up-Regulation Young Adult |
| title | Propionibacterium acnes Activates the NLRP3 Inflammasome in Human Sebocytes |
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