Molecular phylogenetic profiling of gut-associated bacteria in larvae and adults of flesh flies
Flesh flies of the genus Sarcophaga (Diptera: Sarcophagidae) are carrion‐breeding, necrophagous insects important in medical and veterinary entomology as potential transmitters of pathogens to humans and animals. Our aim was to analyse the diversity of gut‐associated bacteria in wild‐caught larvae a...
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| Veröffentlicht in: | Medical and veterinary entomology 2014-12, Vol.28 (4), p.345-354 |
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| creator | GUPTA, A. K. RASTOGI, G. NAYDUCH, D. SAWANT, S. S. BHONDE, R. R. SHOUCHE, Y. S. |
| description | Flesh flies of the genus Sarcophaga (Diptera: Sarcophagidae) are carrion‐breeding, necrophagous insects important in medical and veterinary entomology as potential transmitters of pathogens to humans and animals. Our aim was to analyse the diversity of gut‐associated bacteria in wild‐caught larvae and adult flesh flies using culture‐dependent and culture‐independent methods. Analysis of 16S rRNA gene sequences from cultured isolates and clone libraries revealed bacteria affiliated to Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes in the guts of larval and adult flesh flies. Bacteria cultured from larval and adult flesh fly guts belonged to the genera Acinetobacter, Bacillus, Budvicia, Citrobacter, Dermacoccus, Enterococcus, Ignatzschineria, Lysinibacillus, Myroides, Pasteurella, Proteus, Providencia and Staphylococcus. Phylogenetic analysis showed clone sequences of the genera Aeromonas, Bacillus, Bradyrhizobium, Citrobacter, Clostridium, Corynebacterium, Ignatzschineria, Klebsiella, Pantoea, Propionibacterium, Proteus, Providencia, Serratia, Sporosarcina, Weissella and Wohlfahrtiimonas. Species of clinically significant genera such as Ignatzschineria and Wohlfahrtiimonas spp. were detected in both larvae and adult flesh flies. Sequence analysis of 16S rRNA gene libraries supported culture‐based results and revealed the presence of additional bacterial taxa. This study determined the diversity of gut microbiota in flesh flies, which will bolster the ability to assess microbiological risk associated with the presence of these flies. The present data thereby establish a platform for a much larger study. |
| doi_str_mv | 10.1111/mve.12054 |
| format | Article |
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K. ; RASTOGI, G. ; NAYDUCH, D. ; SAWANT, S. S. ; BHONDE, R. R. ; SHOUCHE, Y. S.</creator><creatorcontrib>GUPTA, A. K. ; RASTOGI, G. ; NAYDUCH, D. ; SAWANT, S. S. ; BHONDE, R. R. ; SHOUCHE, Y. S.</creatorcontrib><description>Flesh flies of the genus Sarcophaga (Diptera: Sarcophagidae) are carrion‐breeding, necrophagous insects important in medical and veterinary entomology as potential transmitters of pathogens to humans and animals. Our aim was to analyse the diversity of gut‐associated bacteria in wild‐caught larvae and adult flesh flies using culture‐dependent and culture‐independent methods. Analysis of 16S rRNA gene sequences from cultured isolates and clone libraries revealed bacteria affiliated to Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes in the guts of larval and adult flesh flies. Bacteria cultured from larval and adult flesh fly guts belonged to the genera Acinetobacter, Bacillus, Budvicia, Citrobacter, Dermacoccus, Enterococcus, Ignatzschineria, Lysinibacillus, Myroides, Pasteurella, Proteus, Providencia and Staphylococcus. Phylogenetic analysis showed clone sequences of the genera Aeromonas, Bacillus, Bradyrhizobium, Citrobacter, Clostridium, Corynebacterium, Ignatzschineria, Klebsiella, Pantoea, Propionibacterium, Proteus, Providencia, Serratia, Sporosarcina, Weissella and Wohlfahrtiimonas. Species of clinically significant genera such as Ignatzschineria and Wohlfahrtiimonas spp. were detected in both larvae and adult flesh flies. Sequence analysis of 16S rRNA gene libraries supported culture‐based results and revealed the presence of additional bacterial taxa. This study determined the diversity of gut microbiota in flesh flies, which will bolster the ability to assess microbiological risk associated with the presence of these flies. The present data thereby establish a platform for a much larger study.</description><identifier>ISSN: 0269-283X</identifier><identifier>EISSN: 1365-2915</identifier><identifier>DOI: 10.1111/mve.12054</identifier><identifier>PMID: 24805263</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>16S rRNA gene ; Acinetobacter ; Actinobacteria ; Aeromonas ; Animals ; Bacillus ; Bacteria ; Bacteria - genetics ; Bacteria - isolation & purification ; Bacterial diversity ; Bradyrhizobium ; Citrobacter ; Cloning ; Clostridium ; Corynebacterium ; Diptera ; Diptera - microbiology ; Enterococcus ; Entomology ; Firmicutes ; flesh fly ; Gastrointestinal Tract - microbiology ; Genes ; insect gut bacteria ; Klebsiella ; Larva - microbiology ; microbial community ; Pasteurella ; Phylogenetics ; Phylogeny ; Propionibacterium ; Proteobacteria ; Proteus ; Providencia ; Sarcophaga ; Sarcophagidae ; Serratia ; Sporosarcina ; Staphylococcus ; Weissella</subject><ispartof>Medical and veterinary entomology, 2014-12, Vol.28 (4), p.345-354</ispartof><rights>2014 The Royal Entomological Society</rights><rights>2014 The Royal Entomological Society.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4504-3d96287e371c48f54d22fc2b64460fc0eaf42d17b1181e8dbb3580eadbe54d4a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fmve.12054$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fmve.12054$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24805263$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GUPTA, A. K.</creatorcontrib><creatorcontrib>RASTOGI, G.</creatorcontrib><creatorcontrib>NAYDUCH, D.</creatorcontrib><creatorcontrib>SAWANT, S. S.</creatorcontrib><creatorcontrib>BHONDE, R. R.</creatorcontrib><creatorcontrib>SHOUCHE, Y. S.</creatorcontrib><title>Molecular phylogenetic profiling of gut-associated bacteria in larvae and adults of flesh flies</title><title>Medical and veterinary entomology</title><addtitle>Med Vet Entomol</addtitle><description>Flesh flies of the genus Sarcophaga (Diptera: Sarcophagidae) are carrion‐breeding, necrophagous insects important in medical and veterinary entomology as potential transmitters of pathogens to humans and animals. Our aim was to analyse the diversity of gut‐associated bacteria in wild‐caught larvae and adult flesh flies using culture‐dependent and culture‐independent methods. Analysis of 16S rRNA gene sequences from cultured isolates and clone libraries revealed bacteria affiliated to Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes in the guts of larval and adult flesh flies. Bacteria cultured from larval and adult flesh fly guts belonged to the genera Acinetobacter, Bacillus, Budvicia, Citrobacter, Dermacoccus, Enterococcus, Ignatzschineria, Lysinibacillus, Myroides, Pasteurella, Proteus, Providencia and Staphylococcus. Phylogenetic analysis showed clone sequences of the genera Aeromonas, Bacillus, Bradyrhizobium, Citrobacter, Clostridium, Corynebacterium, Ignatzschineria, Klebsiella, Pantoea, Propionibacterium, Proteus, Providencia, Serratia, Sporosarcina, Weissella and Wohlfahrtiimonas. Species of clinically significant genera such as Ignatzschineria and Wohlfahrtiimonas spp. were detected in both larvae and adult flesh flies. Sequence analysis of 16S rRNA gene libraries supported culture‐based results and revealed the presence of additional bacterial taxa. This study determined the diversity of gut microbiota in flesh flies, which will bolster the ability to assess microbiological risk associated with the presence of these flies. The present data thereby establish a platform for a much larger study.</description><subject>16S rRNA gene</subject><subject>Acinetobacter</subject><subject>Actinobacteria</subject><subject>Aeromonas</subject><subject>Animals</subject><subject>Bacillus</subject><subject>Bacteria</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>Bacterial diversity</subject><subject>Bradyrhizobium</subject><subject>Citrobacter</subject><subject>Cloning</subject><subject>Clostridium</subject><subject>Corynebacterium</subject><subject>Diptera</subject><subject>Diptera - microbiology</subject><subject>Enterococcus</subject><subject>Entomology</subject><subject>Firmicutes</subject><subject>flesh fly</subject><subject>Gastrointestinal Tract - microbiology</subject><subject>Genes</subject><subject>insect gut bacteria</subject><subject>Klebsiella</subject><subject>Larva - microbiology</subject><subject>microbial community</subject><subject>Pasteurella</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Propionibacterium</subject><subject>Proteobacteria</subject><subject>Proteus</subject><subject>Providencia</subject><subject>Sarcophaga</subject><subject>Sarcophagidae</subject><subject>Serratia</subject><subject>Sporosarcina</subject><subject>Staphylococcus</subject><subject>Weissella</subject><issn>0269-283X</issn><issn>1365-2915</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0U9vFCEYBnBiNHatHvwCZhIvepgWXmCGOZqmf0za6kFb44Uw8LKlsjMrzFT320u7bQ8mJnKAhPzeJ5CHkNeM7rGy9lc3uMeASvGELBhvZA0dk0_JgkLT1aD4tx3yIudrSlnbATwnOyAUldDwBdFnY0Q7R5Oq9dUmjksccAq2WqfRhxiGZTX6ajlPtcl5tMFM6Kre2AlTMFUYqjJ4Y7Ayg6uMm-OUb72PmK_KHjC_JM-8iRlf3Z-75OvR4ZeDk_r00_HHgw-ntRWSipq7rgHVIm-ZFcpL4QC8hb4RoqHeUjRegGNtz5hiqFzfc6nKreuxWGH4Lnm3zS0P_zljnvQqZIsxmgHHOWvWcElBKQn_QQFkJ8tAoW__otfjnIbykTtFgXWUF_V-q2wac07o9TqFlUkbzai-LUiXgvRdQcW-uU-c-xW6R_nQSAH7W_ArRNz8O0mfXRw-RNbbiZAn_P04YdIP3bS8lfry_Fhffj8_Orlgrf7M_wAwPKi7</recordid><startdate>201412</startdate><enddate>201412</enddate><creator>GUPTA, A. K.</creator><creator>RASTOGI, G.</creator><creator>NAYDUCH, D.</creator><creator>SAWANT, S. S.</creator><creator>BHONDE, R. R.</creator><creator>SHOUCHE, Y. S.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>C1K</scope></search><sort><creationdate>201412</creationdate><title>Molecular phylogenetic profiling of gut-associated bacteria in larvae and adults of flesh flies</title><author>GUPTA, A. K. ; RASTOGI, G. ; NAYDUCH, D. ; SAWANT, S. S. ; BHONDE, R. R. ; SHOUCHE, Y. 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K.</au><au>RASTOGI, G.</au><au>NAYDUCH, D.</au><au>SAWANT, S. S.</au><au>BHONDE, R. R.</au><au>SHOUCHE, Y. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular phylogenetic profiling of gut-associated bacteria in larvae and adults of flesh flies</atitle><jtitle>Medical and veterinary entomology</jtitle><addtitle>Med Vet Entomol</addtitle><date>2014-12</date><risdate>2014</risdate><volume>28</volume><issue>4</issue><spage>345</spage><epage>354</epage><pages>345-354</pages><issn>0269-283X</issn><eissn>1365-2915</eissn><abstract>Flesh flies of the genus Sarcophaga (Diptera: Sarcophagidae) are carrion‐breeding, necrophagous insects important in medical and veterinary entomology as potential transmitters of pathogens to humans and animals. Our aim was to analyse the diversity of gut‐associated bacteria in wild‐caught larvae and adult flesh flies using culture‐dependent and culture‐independent methods. Analysis of 16S rRNA gene sequences from cultured isolates and clone libraries revealed bacteria affiliated to Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes in the guts of larval and adult flesh flies. Bacteria cultured from larval and adult flesh fly guts belonged to the genera Acinetobacter, Bacillus, Budvicia, Citrobacter, Dermacoccus, Enterococcus, Ignatzschineria, Lysinibacillus, Myroides, Pasteurella, Proteus, Providencia and Staphylococcus. Phylogenetic analysis showed clone sequences of the genera Aeromonas, Bacillus, Bradyrhizobium, Citrobacter, Clostridium, Corynebacterium, Ignatzschineria, Klebsiella, Pantoea, Propionibacterium, Proteus, Providencia, Serratia, Sporosarcina, Weissella and Wohlfahrtiimonas. Species of clinically significant genera such as Ignatzschineria and Wohlfahrtiimonas spp. were detected in both larvae and adult flesh flies. Sequence analysis of 16S rRNA gene libraries supported culture‐based results and revealed the presence of additional bacterial taxa. This study determined the diversity of gut microbiota in flesh flies, which will bolster the ability to assess microbiological risk associated with the presence of these flies. The present data thereby establish a platform for a much larger study.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>24805263</pmid><doi>10.1111/mve.12054</doi><tpages>10</tpages></addata></record> |
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| ispartof | Medical and veterinary entomology, 2014-12, Vol.28 (4), p.345-354 |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE |
| subjects | 16S rRNA gene Acinetobacter Actinobacteria Aeromonas Animals Bacillus Bacteria Bacteria - genetics Bacteria - isolation & purification Bacterial diversity Bradyrhizobium Citrobacter Cloning Clostridium Corynebacterium Diptera Diptera - microbiology Enterococcus Entomology Firmicutes flesh fly Gastrointestinal Tract - microbiology Genes insect gut bacteria Klebsiella Larva - microbiology microbial community Pasteurella Phylogenetics Phylogeny Propionibacterium Proteobacteria Proteus Providencia Sarcophaga Sarcophagidae Serratia Sporosarcina Staphylococcus Weissella |
| title | Molecular phylogenetic profiling of gut-associated bacteria in larvae and adults of flesh flies |
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