Rapid and efficient proteolysis through laser-assisted immobilized enzyme reactors

► The near-IR laser radiation can accelerate the proteolysis efficiency through IMER significantly. ► The whole process can be completed in 1 min and room temperature. ► Satisfying analysis of human serum sample using laser-assisted IMER proteolysis. In this report, laser radiation (808 nm) for the...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of Chromatography A 2011-11, Vol.1218 (47), p.8567-8571
Hauptverfasser:	Zhang, Peng, Gao, Mingxia, Zhu, Shaochun, Lei, Jie, Zhang, Xiangmin
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals beta-casein Biological and medical sciences Bioreactors Biotechnology Blood Proteins - analysis Blood Proteins - metabolism blood serum bovine serum albumin Chromatography, Ion Exchange Chromatography, Reverse-Phase cytochrome c digestion Enzymes, Immobilized - chemistry Enzymes, Immobilized - metabolism Fundamental and applied biological sciences. Psychology General aspects, investigation methods Humans Immobilized enzyme reactors immobilized enzymes Investigative techniques, diagnostic techniques (general aspects) Laser Lasers liquid chromatography Mass spectrometry matrix-assisted laser desorption-ionization mass spectrometry Medical sciences Methods. Procedures. Technologies Miscellaneous. Technology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Peptide Fragments - analysis Peptide Fragments - metabolism peptides polymerization Proteins Proteins - analysis Proteins - metabolism Proteolysis Proteome Proteomics - instrumentation Proteomics - methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Time Factors trypsin Trypsin - chemistry Trypsin - metabolism Various methods and equipments
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!