Analytical and preparative separation of PEGylated lysozyme for the characterization of chromatography media

The effect of PEGylation on cation exchange chromatography was studied with poly(ethylene glycol) of different chain lengths (5 kDa, 10 kDa and 30 kDa) using lysozyme as a model system. A stable binding via reduction of a Schiff base was formed during random PEGylation on lysine residues with methox...

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Veröffentlicht in:	Journal of Chromatography A 2010-01, Vol.1217 (2), p.209-215
Hauptverfasser:	Moosmann, Anna, Christel, Jessica, Boettinger, Heiner, Mueller, Egbert
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical separation Cation exchange chromatography Cation Exchange Resins - chemistry Chromatography, Gel Chromatography, Ion Exchange - methods Dynamic binding capacity Electrophoresis, Polyacrylamide Gel Hydrogen-Ion Concentration Lysozyme Muramidase - chemistry Muramidase - isolation & purification PEG PEGylation Polyethylene Glycols - chemistry Polyethylene Glycols - isolation & purification Preparative separation Protein Binding Selectivity Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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