Simple solution for a complex problem: Proanthocyanidins, galloyl glucoses and ellagitannins fit on a single calibration curve in high performance-gel permeation chromatography
This study was undertaken to explore gel permeation chromatography (GPC) for estimating molecular weights of proanthocyanidin fractions isolated from sainfoin ( Onobrychis viciifolia). The results were compared with data obtained by thiolytic degradation of the same fractions. Polystyrene, polyethyl...
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| Veröffentlicht in: | Journal of Chromatography A 2011-10, Vol.1218 (43), p.7804-7812 |
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| creator | Stringano, Elisabetta Gea, An Salminen, Juha-Pekka Mueller-Harvey, Irene |
| description | This study was undertaken to explore gel permeation chromatography (GPC) for estimating molecular weights of proanthocyanidin fractions isolated from sainfoin (
Onobrychis viciifolia). The results were compared with data obtained by thiolytic degradation of the same fractions. Polystyrene, polyethylene glycol and polymethyl methacrylate standards were not suitable for estimating the molecular weights of underivatized proanthocyanidins. Therefore, a novel HPLC–GPC method was developed based on two serially connected PolarGel-L columns using DMF that contained 5% water, 1% acetic acid and 0.15
M LiBr at 0.7
ml/min and 50
°C. This yielded a single calibration curve for galloyl glucoses (trigalloyl glucose, pentagalloyl glucose), ellagitannins (pedunculagin, vescalagin, punicalagin, oenothein B, gemin A), proanthocyanidins (procyanidin B2, cinnamtannin B1), and several other polyphenols (catechin, epicatechin gallate, epicallocatechin gallate, amentoflavone). These GPC predicted molecular weights represented a considerable advance over previously reported HPLC–GPC methods for underivatized proanthocyanidins. |
| doi_str_mv | 10.1016/j.chroma.2011.08.082 |
| format | Article |
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Onobrychis viciifolia). The results were compared with data obtained by thiolytic degradation of the same fractions. Polystyrene, polyethylene glycol and polymethyl methacrylate standards were not suitable for estimating the molecular weights of underivatized proanthocyanidins. Therefore, a novel HPLC–GPC method was developed based on two serially connected PolarGel-L columns using DMF that contained 5% water, 1% acetic acid and 0.15
M LiBr at 0.7
ml/min and 50
°C. This yielded a single calibration curve for galloyl glucoses (trigalloyl glucose, pentagalloyl glucose), ellagitannins (pedunculagin, vescalagin, punicalagin, oenothein B, gemin A), proanthocyanidins (procyanidin B2, cinnamtannin B1), and several other polyphenols (catechin, epicatechin gallate, epicallocatechin gallate, amentoflavone). These GPC predicted molecular weights represented a considerable advance over previously reported HPLC–GPC methods for underivatized proanthocyanidins.</description><identifier>ISSN: 0021-9673</identifier><identifier>EISSN: 1873-3778</identifier><identifier>DOI: 10.1016/j.chroma.2011.08.082</identifier><identifier>PMID: 21930278</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>acetic acid ; Analytical chemistry ; Biological and medical sciences ; Calibration ; Calibration curve ; catechin ; Chemical constitution ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Chromatography, Gel - methods ; Chromatography, Gel - standards ; Chromatography, High Pressure Liquid ; epicatechin ; Exact sciences and technology ; Fabaceae - chemistry ; Fundamental and applied biological sciences. Psychology ; gel chromatography ; glucose ; GPC ; Hydrolyzable Tannins - analysis ; Hydrolyzable Tannins - chemistry ; Linear Models ; Molecular Weight ; Onobrychis viciifolia ; Other chromatographic methods ; Plant physiology and development ; polyethylene glycol ; polymethylmetacrylate ; Polyphenols - analysis ; Polyphenols - chemistry ; polystyrenes ; Proanthocyanidins ; Proanthocyanidins - analysis ; Proanthocyanidins - chemistry ; procyanidins ; Reference Standards ; Reproducibility of Results ; Tannins ; Thiolytic degradation</subject><ispartof>Journal of Chromatography A, 2011-10, Vol.1218 (43), p.7804-7812</ispartof><rights>2011 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c514t-1c0c4df8ebaae4ca3cc7e34e40fd990b2a0356084049bfd2a5ea91eb2c38320e3</citedby><cites>FETCH-LOGICAL-c514t-1c0c4df8ebaae4ca3cc7e34e40fd990b2a0356084049bfd2a5ea91eb2c38320e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.chroma.2011.08.082$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24604018$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21930278$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stringano, Elisabetta</creatorcontrib><creatorcontrib>Gea, An</creatorcontrib><creatorcontrib>Salminen, Juha-Pekka</creatorcontrib><creatorcontrib>Mueller-Harvey, Irene</creatorcontrib><title>Simple solution for a complex problem: Proanthocyanidins, galloyl glucoses and ellagitannins fit on a single calibration curve in high performance-gel permeation chromatography</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr A</addtitle><description>This study was undertaken to explore gel permeation chromatography (GPC) for estimating molecular weights of proanthocyanidin fractions isolated from sainfoin (
Onobrychis viciifolia). The results were compared with data obtained by thiolytic degradation of the same fractions. Polystyrene, polyethylene glycol and polymethyl methacrylate standards were not suitable for estimating the molecular weights of underivatized proanthocyanidins. Therefore, a novel HPLC–GPC method was developed based on two serially connected PolarGel-L columns using DMF that contained 5% water, 1% acetic acid and 0.15
M LiBr at 0.7
ml/min and 50
°C. This yielded a single calibration curve for galloyl glucoses (trigalloyl glucose, pentagalloyl glucose), ellagitannins (pedunculagin, vescalagin, punicalagin, oenothein B, gemin A), proanthocyanidins (procyanidin B2, cinnamtannin B1), and several other polyphenols (catechin, epicatechin gallate, epicallocatechin gallate, amentoflavone). These GPC predicted molecular weights represented a considerable advance over previously reported HPLC–GPC methods for underivatized proanthocyanidins.</description><subject>acetic acid</subject><subject>Analytical chemistry</subject><subject>Biological and medical sciences</subject><subject>Calibration</subject><subject>Calibration curve</subject><subject>catechin</subject><subject>Chemical constitution</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Chromatography, Gel - methods</subject><subject>Chromatography, Gel - standards</subject><subject>Chromatography, High Pressure Liquid</subject><subject>epicatechin</subject><subject>Exact sciences and technology</subject><subject>Fabaceae - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gel chromatography</subject><subject>glucose</subject><subject>GPC</subject><subject>Hydrolyzable Tannins - analysis</subject><subject>Hydrolyzable Tannins - chemistry</subject><subject>Linear Models</subject><subject>Molecular Weight</subject><subject>Onobrychis viciifolia</subject><subject>Other chromatographic methods</subject><subject>Plant physiology and development</subject><subject>polyethylene glycol</subject><subject>polymethylmetacrylate</subject><subject>Polyphenols - analysis</subject><subject>Polyphenols - chemistry</subject><subject>polystyrenes</subject><subject>Proanthocyanidins</subject><subject>Proanthocyanidins - analysis</subject><subject>Proanthocyanidins - chemistry</subject><subject>procyanidins</subject><subject>Reference Standards</subject><subject>Reproducibility of Results</subject><subject>Tannins</subject><subject>Thiolytic degradation</subject><issn>0021-9673</issn><issn>1873-3778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kd-L1DAQx4so3nr6H4jmRbgHu06SdtvegyCHv-BA4bznME2n3Sxpsibt4f5X9yea3q76JgyETD4z38x3suwlhzUHvnm3W-tt8COuBXC-hjqFeJSteF3JXFZV_ThbAQieN5tKnmXPYtwB8Aoq8TQ7E7yRIKp6ld3fmHFviUVv58l4x3ofGDLtl-wvtg--tTResu_Bo5u2Xh_Qmc64-JYNaK0_WDbYWftIkaHrGFmLg5nQucSw3kws9UQWjRuSikZr2oAPQnoOd8SMY1szbNmeQlIe0WnKB7LLfaQT-DDm5IeA--3hefakRxvpxek8z24_ffxx9SW__vb569WH61yXvJhyrkEXXV9Ti0iFRql1RbKgAvquaaAVCLLcQF1A0bR9J7AkbDi1QstaCiB5nl0c-yYLfs4UJzWaqJfxHPk5Kr6RJQgohEhocUR18DEG6tU-mBHDQXFQy67UTh2HUMuuFNQplrJXJ4W5Han7W_RnOQl4cwIwJuf6kNwx8R9XbKAAvnCvj1yPXuEQEnN7k5RKWF55uRDvjwQlx-4MBRW1oeR1ZwLpSXXe_P-vvwGIA8LC</recordid><startdate>20111028</startdate><enddate>20111028</enddate><creator>Stringano, Elisabetta</creator><creator>Gea, An</creator><creator>Salminen, Juha-Pekka</creator><creator>Mueller-Harvey, Irene</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope></search><sort><creationdate>20111028</creationdate><title>Simple solution for a complex problem: Proanthocyanidins, galloyl glucoses and ellagitannins fit on a single calibration curve in high performance-gel permeation chromatography</title><author>Stringano, Elisabetta ; Gea, An ; Salminen, Juha-Pekka ; Mueller-Harvey, Irene</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c514t-1c0c4df8ebaae4ca3cc7e34e40fd990b2a0356084049bfd2a5ea91eb2c38320e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>acetic acid</topic><topic>Analytical chemistry</topic><topic>Biological and medical sciences</topic><topic>Calibration</topic><topic>Calibration curve</topic><topic>catechin</topic><topic>Chemical constitution</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Chromatography, Gel - methods</topic><topic>Chromatography, Gel - standards</topic><topic>Chromatography, High Pressure Liquid</topic><topic>epicatechin</topic><topic>Exact sciences and technology</topic><topic>Fabaceae - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gel chromatography</topic><topic>glucose</topic><topic>GPC</topic><topic>Hydrolyzable Tannins - analysis</topic><topic>Hydrolyzable Tannins - chemistry</topic><topic>Linear Models</topic><topic>Molecular Weight</topic><topic>Onobrychis viciifolia</topic><topic>Other chromatographic methods</topic><topic>Plant physiology and development</topic><topic>polyethylene glycol</topic><topic>polymethylmetacrylate</topic><topic>Polyphenols - analysis</topic><topic>Polyphenols - chemistry</topic><topic>polystyrenes</topic><topic>Proanthocyanidins</topic><topic>Proanthocyanidins - analysis</topic><topic>Proanthocyanidins - chemistry</topic><topic>procyanidins</topic><topic>Reference Standards</topic><topic>Reproducibility of Results</topic><topic>Tannins</topic><topic>Thiolytic degradation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stringano, Elisabetta</creatorcontrib><creatorcontrib>Gea, An</creatorcontrib><creatorcontrib>Salminen, Juha-Pekka</creatorcontrib><creatorcontrib>Mueller-Harvey, Irene</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stringano, Elisabetta</au><au>Gea, An</au><au>Salminen, Juha-Pekka</au><au>Mueller-Harvey, Irene</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simple solution for a complex problem: Proanthocyanidins, galloyl glucoses and ellagitannins fit on a single calibration curve in high performance-gel permeation chromatography</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>2011-10-28</date><risdate>2011</risdate><volume>1218</volume><issue>43</issue><spage>7804</spage><epage>7812</epage><pages>7804-7812</pages><issn>0021-9673</issn><eissn>1873-3778</eissn><coden>JOCRAM</coden><abstract>This study was undertaken to explore gel permeation chromatography (GPC) for estimating molecular weights of proanthocyanidin fractions isolated from sainfoin (
Onobrychis viciifolia). The results were compared with data obtained by thiolytic degradation of the same fractions. Polystyrene, polyethylene glycol and polymethyl methacrylate standards were not suitable for estimating the molecular weights of underivatized proanthocyanidins. Therefore, a novel HPLC–GPC method was developed based on two serially connected PolarGel-L columns using DMF that contained 5% water, 1% acetic acid and 0.15
M LiBr at 0.7
ml/min and 50
°C. This yielded a single calibration curve for galloyl glucoses (trigalloyl glucose, pentagalloyl glucose), ellagitannins (pedunculagin, vescalagin, punicalagin, oenothein B, gemin A), proanthocyanidins (procyanidin B2, cinnamtannin B1), and several other polyphenols (catechin, epicatechin gallate, epicallocatechin gallate, amentoflavone). These GPC predicted molecular weights represented a considerable advance over previously reported HPLC–GPC methods for underivatized proanthocyanidins.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>21930278</pmid><doi>10.1016/j.chroma.2011.08.082</doi><tpages>9</tpages></addata></record> |
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| subjects | acetic acid Analytical chemistry Biological and medical sciences Calibration Calibration curve catechin Chemical constitution Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography, Gel - methods Chromatography, Gel - standards Chromatography, High Pressure Liquid epicatechin Exact sciences and technology Fabaceae - chemistry Fundamental and applied biological sciences. Psychology gel chromatography glucose GPC Hydrolyzable Tannins - analysis Hydrolyzable Tannins - chemistry Linear Models Molecular Weight Onobrychis viciifolia Other chromatographic methods Plant physiology and development polyethylene glycol polymethylmetacrylate Polyphenols - analysis Polyphenols - chemistry polystyrenes Proanthocyanidins Proanthocyanidins - analysis Proanthocyanidins - chemistry procyanidins Reference Standards Reproducibility of Results Tannins Thiolytic degradation |
| title | Simple solution for a complex problem: Proanthocyanidins, galloyl glucoses and ellagitannins fit on a single calibration curve in high performance-gel permeation chromatography |
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