Exploration of overloaded cation exchange chromatography for monoclonal antibody purification

Cation exchange chromatography using conventional resins, having either diffusive or perfusive flow paths, operated in bind-elute mode has been commonly employed in monoclonal antibody (MAb) purification processes. In this study, the performance of diffusive and perfusive cation exchange resins (SP-...

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Veröffentlicht in:	Journal of Chromatography A 2011-09, Vol.1218 (39), p.6943-6952
Hauptverfasser:	Liu, Hui F., McCooey, Beth, Duarte, Tiago, Myers, Deanna E., Hudson, Terry, Amanullah, Ashraf, van Reis, Robert, Kelley, Brian D.
Format:	Artikel
Sprache:	eng
Schlagworte:	affinity chromatography Analytical, structural and metabolic biochemistry Animals anion exchange chromatography Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - isolation & purification Antibodies, Monoclonal - metabolism binding capacity Biological and medical sciences Cation exchange cation exchange capacity cation exchange chromatography Cation Exchange Resins cell culture CHO Cells Chromatography Chromatography, Affinity - methods Chromatography, Ion Exchange - methods Conventional and convective chromatography matrices Cricetinae Cricetulus DNA DNA - chemistry DNA - isolation & purification Dynamic binding capacity Fundamental and applied biological sciences. Psychology General pharmacology gentamicin Glycoproteins High molecular weight species Humans Immunoglobulin G - chemistry Immunoglobulin G - isolation & purification Immunoglobulin G - metabolism manufacturing Medical sciences Membranes, Artificial Molecular Weight monoclonal antibodies Monoclonal antibody purification Overloaded chromatography Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Protein Binding Proteins Proteins - chemistry Proteins - isolation & purification purification methods Staphylococcal Protein A - chemistry
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