MicroRNA-145 directly targets the insulin-like growth factor receptor I in human bladder cancer cells
•miR-145 levels are decreased in human bladder cancer cells.•miR-145 expression affects IGF-IR levels and IGF-I-induced cell viability.•miR-145 promotes apoptosis and suppresses migration in T24 cells.•Silencing of IGF-IR increases cell apoptosis and inhibits proliferation and migration.•miR-145 fun...
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| Veröffentlicht in: | FEBS letters 2014-08, Vol.588 (17), p.3180-3185 |
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| creator | Zhu, Zhaowei Xu, Tianyuan Wang, Li Wang, Xianjin Zhong, Shan Xu, Chen Shen, Zhoujun |
| description | •miR-145 levels are decreased in human bladder cancer cells.•miR-145 expression affects IGF-IR levels and IGF-I-induced cell viability.•miR-145 promotes apoptosis and suppresses migration in T24 cells.•Silencing of IGF-IR increases cell apoptosis and inhibits proliferation and migration.•miR-145 functions as a tumour suppressor through repression of oncogenic IGF-IR.
The insulin-like growth factor receptor I (IGF-IR) is a proto-oncogene with potent mitogenic and antiapoptotic activities. It has been reported that expression of IGF-IR is up-regulated in bladder cancer. Here, we assessed whether microRNA-145 (miR-145) regulates IGF-IR expression in bladder cancer. In our study, miR-145 was shown to directly target IGF-IR 3′-untranslated region (UTR) in human bladder cancer cells. Small interfering RNA (siRNA)- and miR-145-mediated IGF-IR knockdown experiments revealed that miR-145 promotes cell apoptosis, and suppresses cell proliferation and migration through suppression of IGF-IR expression. Taken together, our data suggest that miR-145 may inhibit bladder cancer initiation by affecting IGF-IR signaling. |
| doi_str_mv | 10.1016/j.febslet.2014.06.059 |
| format | Article |
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The insulin-like growth factor receptor I (IGF-IR) is a proto-oncogene with potent mitogenic and antiapoptotic activities. It has been reported that expression of IGF-IR is up-regulated in bladder cancer. Here, we assessed whether microRNA-145 (miR-145) regulates IGF-IR expression in bladder cancer. In our study, miR-145 was shown to directly target IGF-IR 3′-untranslated region (UTR) in human bladder cancer cells. Small interfering RNA (siRNA)- and miR-145-mediated IGF-IR knockdown experiments revealed that miR-145 promotes cell apoptosis, and suppresses cell proliferation and migration through suppression of IGF-IR expression. Taken together, our data suggest that miR-145 may inhibit bladder cancer initiation by affecting IGF-IR signaling.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2014.06.059</identifier><identifier>PMID: 24999188</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>3' Untranslated Regions - genetics ; Apoptosis ; Apoptosis - genetics ; Base Sequence ; Bladder cancer ; Cell Line, Tumor ; Cell Movement - genetics ; Cell Proliferation ; Gene Expression Regulation, Neoplastic ; Gene Knockdown Techniques ; Gene Silencing ; Humans ; IGF-IR ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Migration ; miR-145 ; Proliferation ; Receptor, IGF Type 1 - deficiency ; Receptor, IGF Type 1 - genetics ; RNA, Small Interfering - genetics ; Urinary Bladder Neoplasms - genetics ; Urinary Bladder Neoplasms - pathology</subject><ispartof>FEBS letters, 2014-08, Vol.588 (17), p.3180-3185</ispartof><rights>2014</rights><rights>FEBS Letters 588 (2014) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>Copyright © 2014. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6099-da41b114ec44ee012a15cfa78fc2192acc46b0218f0b5a81b8df201197e57f223</citedby><cites>FETCH-LOGICAL-c6099-da41b114ec44ee012a15cfa78fc2192acc46b0218f0b5a81b8df201197e57f223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2014.06.059$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.febslet.2014.06.059$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>315,781,785,1418,1434,3551,27929,27930,45579,45580,46000,46414,46838</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24999188$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Zhaowei</creatorcontrib><creatorcontrib>Xu, Tianyuan</creatorcontrib><creatorcontrib>Wang, Li</creatorcontrib><creatorcontrib>Wang, Xianjin</creatorcontrib><creatorcontrib>Zhong, Shan</creatorcontrib><creatorcontrib>Xu, Chen</creatorcontrib><creatorcontrib>Shen, Zhoujun</creatorcontrib><title>MicroRNA-145 directly targets the insulin-like growth factor receptor I in human bladder cancer cells</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>•miR-145 levels are decreased in human bladder cancer cells.•miR-145 expression affects IGF-IR levels and IGF-I-induced cell viability.•miR-145 promotes apoptosis and suppresses migration in T24 cells.•Silencing of IGF-IR increases cell apoptosis and inhibits proliferation and migration.•miR-145 functions as a tumour suppressor through repression of oncogenic IGF-IR.
The insulin-like growth factor receptor I (IGF-IR) is a proto-oncogene with potent mitogenic and antiapoptotic activities. It has been reported that expression of IGF-IR is up-regulated in bladder cancer. Here, we assessed whether microRNA-145 (miR-145) regulates IGF-IR expression in bladder cancer. In our study, miR-145 was shown to directly target IGF-IR 3′-untranslated region (UTR) in human bladder cancer cells. Small interfering RNA (siRNA)- and miR-145-mediated IGF-IR knockdown experiments revealed that miR-145 promotes cell apoptosis, and suppresses cell proliferation and migration through suppression of IGF-IR expression. Taken together, our data suggest that miR-145 may inhibit bladder cancer initiation by affecting IGF-IR signaling.</description><subject>3' Untranslated Regions - genetics</subject><subject>Apoptosis</subject><subject>Apoptosis - genetics</subject><subject>Base Sequence</subject><subject>Bladder cancer</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - genetics</subject><subject>Cell Proliferation</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Gene Knockdown Techniques</subject><subject>Gene Silencing</subject><subject>Humans</subject><subject>IGF-IR</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Migration</subject><subject>miR-145</subject><subject>Proliferation</subject><subject>Receptor, IGF Type 1 - deficiency</subject><subject>Receptor, IGF Type 1 - genetics</subject><subject>RNA, Small Interfering - genetics</subject><subject>Urinary Bladder Neoplasms - genetics</subject><subject>Urinary Bladder Neoplasms - pathology</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9v1DAQxS0EokvhI4B85JLgcezEPqFStbRSAYk_Z8txJl0v3mSxE6r99jjKwrWcxpbfvBm_HyGvgZXAoH63K3tsU8Cp5AxEyeqSSf2EbEA1VVGJWj0lG5ZfCtno6oy8SGnH8l2Bfk7OuNBag1Ibgp-8i-PXzxcFCEk7H9FN4UgnG-9xSnTaIvVDmoMfiuB_Ir2P48O0pb110xhpVuNhOdxmFd3OezvQNtiuw0idHdxSMIT0kjzrbUj46lTPyY_rq--XN8Xdl4-3lxd3hauZ1kVnBbQAAp0QiAy4Bel626jecdDcOifqlnFQPWulVdCqrs-_B92gbHrOq3PydvU9xPHXjGkye5-WDeyA45wM1JVkUDXQPC6VUgiptVpc5SrNSaUUsTeH6Pc2Hg0ws8AwO3OCYRYYhtUmw8h9b04j5naP3b-uv-lnwc0qePABj__naq6vPvBvC9kFLAjGZPbLVu9XK8zx_vYYTXIeM4EVqelG_8i2fwDvz7Jw</recordid><startdate>20140825</startdate><enddate>20140825</enddate><creator>Zhu, Zhaowei</creator><creator>Xu, Tianyuan</creator><creator>Wang, Li</creator><creator>Wang, Xianjin</creator><creator>Zhong, Shan</creator><creator>Xu, Chen</creator><creator>Shen, Zhoujun</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope></search><sort><creationdate>20140825</creationdate><title>MicroRNA-145 directly targets the insulin-like growth factor receptor I in human bladder cancer cells</title><author>Zhu, Zhaowei ; Xu, Tianyuan ; Wang, Li ; Wang, Xianjin ; Zhong, Shan ; Xu, Chen ; Shen, Zhoujun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6099-da41b114ec44ee012a15cfa78fc2192acc46b0218f0b5a81b8df201197e57f223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>3' Untranslated Regions - genetics</topic><topic>Apoptosis</topic><topic>Apoptosis - genetics</topic><topic>Base Sequence</topic><topic>Bladder cancer</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement - genetics</topic><topic>Cell Proliferation</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Gene Knockdown Techniques</topic><topic>Gene Silencing</topic><topic>Humans</topic><topic>IGF-IR</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Migration</topic><topic>miR-145</topic><topic>Proliferation</topic><topic>Receptor, IGF Type 1 - deficiency</topic><topic>Receptor, IGF Type 1 - genetics</topic><topic>RNA, Small Interfering - genetics</topic><topic>Urinary Bladder Neoplasms - genetics</topic><topic>Urinary Bladder Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Zhaowei</creatorcontrib><creatorcontrib>Xu, Tianyuan</creatorcontrib><creatorcontrib>Wang, Li</creatorcontrib><creatorcontrib>Wang, Xianjin</creatorcontrib><creatorcontrib>Zhong, Shan</creatorcontrib><creatorcontrib>Xu, Chen</creatorcontrib><creatorcontrib>Shen, Zhoujun</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Zhaowei</au><au>Xu, Tianyuan</au><au>Wang, Li</au><au>Wang, Xianjin</au><au>Zhong, Shan</au><au>Xu, Chen</au><au>Shen, Zhoujun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MicroRNA-145 directly targets the insulin-like growth factor receptor I in human bladder cancer cells</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2014-08-25</date><risdate>2014</risdate><volume>588</volume><issue>17</issue><spage>3180</spage><epage>3185</epage><pages>3180-3185</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>•miR-145 levels are decreased in human bladder cancer cells.•miR-145 expression affects IGF-IR levels and IGF-I-induced cell viability.•miR-145 promotes apoptosis and suppresses migration in T24 cells.•Silencing of IGF-IR increases cell apoptosis and inhibits proliferation and migration.•miR-145 functions as a tumour suppressor through repression of oncogenic IGF-IR.
The insulin-like growth factor receptor I (IGF-IR) is a proto-oncogene with potent mitogenic and antiapoptotic activities. It has been reported that expression of IGF-IR is up-regulated in bladder cancer. Here, we assessed whether microRNA-145 (miR-145) regulates IGF-IR expression in bladder cancer. In our study, miR-145 was shown to directly target IGF-IR 3′-untranslated region (UTR) in human bladder cancer cells. Small interfering RNA (siRNA)- and miR-145-mediated IGF-IR knockdown experiments revealed that miR-145 promotes cell apoptosis, and suppresses cell proliferation and migration through suppression of IGF-IR expression. Taken together, our data suggest that miR-145 may inhibit bladder cancer initiation by affecting IGF-IR signaling.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>24999188</pmid><doi>10.1016/j.febslet.2014.06.059</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 3' Untranslated Regions - genetics Apoptosis Apoptosis - genetics Base Sequence Bladder cancer Cell Line, Tumor Cell Movement - genetics Cell Proliferation Gene Expression Regulation, Neoplastic Gene Knockdown Techniques Gene Silencing Humans IGF-IR MicroRNAs - genetics MicroRNAs - metabolism Migration miR-145 Proliferation Receptor, IGF Type 1 - deficiency Receptor, IGF Type 1 - genetics RNA, Small Interfering - genetics Urinary Bladder Neoplasms - genetics Urinary Bladder Neoplasms - pathology |
| title | MicroRNA-145 directly targets the insulin-like growth factor receptor I in human bladder cancer cells |
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