Phenolic Compounds Involved in Grafting Incompatibility of Vitis spp: Development and Validation of an Analytical Method for their Quantification
INTRODUCTION: Graft incompatibility of Vitis spp is an unresolved worldwide problem with important economic consequences. Grafting comprises a complex set of morphological and physiological alterations, in which the phenolic compounds seem to be strongly involved. Therefore, a detailed analysis and...
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| Veröffentlicht in: | Phytochemical analysis 2015-01, Vol.26 (1), p.1-7 |
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| creator | Canas, Sara Assunção, Maria Brazão, João Zanol, Geni Eiras‐Dias, José Eduardo |
| description | INTRODUCTION: Graft incompatibility of Vitis spp is an unresolved worldwide problem with important economic consequences. Grafting comprises a complex set of morphological and physiological alterations, in which the phenolic compounds seem to be strongly involved. Therefore, a detailed analysis and recognition of structural phenolic compounds diversity in the two partners of a Vitis graft is of great importance to evaluate their role as markers of graft establishment. OBJECTIVE: To optimise a sample extraction method, and to develop and validate a high‐performance liquid chromatography (HPLC) method for the simultaneous determination of phenolic acids and flavonols in the graft union so as to understand their behaviour in the metabolism of the scion–rootstock system, using compatible and incompatible combinations of a Syrah cultivar and two rootstocks (R110 and SO4). METHODS: Sixty extracts of Vitis grafting tissues were prepared and analysed by HPLC for the qualitative and quantitative determination of their phenolic profile. RESULTS: Among the phenolic compounds identified in the samples, one benzoic acid (gallic acid), three cinnamic acids (caffeic acid, ferulic acid and sinapic acid) and two flavonols (catechin and epicatechin) are potentially suitable as markers of graft incompatibility. CONCLUSION: The method developed presents good performance and lends itself readily for application in routine analysis of the phenolic composition of Vitis grafting tissues to distinguish compatible and incompatible combinations in the graft callusing stage. Copyright © 2014 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/pca.2526 |
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Grafting comprises a complex set of morphological and physiological alterations, in which the phenolic compounds seem to be strongly involved. Therefore, a detailed analysis and recognition of structural phenolic compounds diversity in the two partners of a Vitis graft is of great importance to evaluate their role as markers of graft establishment. OBJECTIVE: To optimise a sample extraction method, and to develop and validate a high‐performance liquid chromatography (HPLC) method for the simultaneous determination of phenolic acids and flavonols in the graft union so as to understand their behaviour in the metabolism of the scion–rootstock system, using compatible and incompatible combinations of a Syrah cultivar and two rootstocks (R110 and SO4). METHODS: Sixty extracts of Vitis grafting tissues were prepared and analysed by HPLC for the qualitative and quantitative determination of their phenolic profile. RESULTS: Among the phenolic compounds identified in the samples, one benzoic acid (gallic acid), three cinnamic acids (caffeic acid, ferulic acid and sinapic acid) and two flavonols (catechin and epicatechin) are potentially suitable as markers of graft incompatibility. CONCLUSION: The method developed presents good performance and lends itself readily for application in routine analysis of the phenolic composition of Vitis grafting tissues to distinguish compatible and incompatible combinations in the graft callusing stage. Copyright © 2014 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0958-0344</identifier><identifier>EISSN: 1099-1565</identifier><identifier>DOI: 10.1002/pca.2526</identifier><identifier>PMID: 24888592</identifier><identifier>CODEN: PHANEL</identifier><language>eng</language><publisher>England: Wiley</publisher><subject>benzoic acid ; caffeic acid ; catechin ; Chromatography, High Pressure Liquid - methods ; Cultivars ; epicatechin ; ferulic acid ; flavonols ; gallic acid ; graft compatibility ; graft incompatibility ; high performance liquid chromatography ; HPLC method ; Liquid chromatography ; metabolism ; phenolic compounds ; Phenols ; Phenols - analysis ; Phenols - isolation & purification ; Plant Extracts - chemistry ; Plant Extracts - isolation & purification ; quantitative analysis ; rootstocks ; sample extraction method ; sinapic acid ; tissues ; Vitis ; Vitis - chemistry ; Vitis spp</subject><ispartof>Phytochemical analysis, 2015-01, Vol.26 (1), p.1-7</ispartof><rights>Copyright © 2014 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4446-d1c939217471c69329af4184242022ea7ba8e04cd2b89f27a91310c5a7226b573</citedby><cites>FETCH-LOGICAL-c4446-d1c939217471c69329af4184242022ea7ba8e04cd2b89f27a91310c5a7226b573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpca.2526$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpca.2526$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24888592$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Canas, Sara</creatorcontrib><creatorcontrib>Assunção, Maria</creatorcontrib><creatorcontrib>Brazão, João</creatorcontrib><creatorcontrib>Zanol, Geni</creatorcontrib><creatorcontrib>Eiras‐Dias, José Eduardo</creatorcontrib><title>Phenolic Compounds Involved in Grafting Incompatibility of Vitis spp: Development and Validation of an Analytical Method for their Quantification</title><title>Phytochemical analysis</title><addtitle>Phytochem. Anal</addtitle><description>INTRODUCTION: Graft incompatibility of Vitis spp is an unresolved worldwide problem with important economic consequences. Grafting comprises a complex set of morphological and physiological alterations, in which the phenolic compounds seem to be strongly involved. Therefore, a detailed analysis and recognition of structural phenolic compounds diversity in the two partners of a Vitis graft is of great importance to evaluate their role as markers of graft establishment. OBJECTIVE: To optimise a sample extraction method, and to develop and validate a high‐performance liquid chromatography (HPLC) method for the simultaneous determination of phenolic acids and flavonols in the graft union so as to understand their behaviour in the metabolism of the scion–rootstock system, using compatible and incompatible combinations of a Syrah cultivar and two rootstocks (R110 and SO4). METHODS: Sixty extracts of Vitis grafting tissues were prepared and analysed by HPLC for the qualitative and quantitative determination of their phenolic profile. RESULTS: Among the phenolic compounds identified in the samples, one benzoic acid (gallic acid), three cinnamic acids (caffeic acid, ferulic acid and sinapic acid) and two flavonols (catechin and epicatechin) are potentially suitable as markers of graft incompatibility. CONCLUSION: The method developed presents good performance and lends itself readily for application in routine analysis of the phenolic composition of Vitis grafting tissues to distinguish compatible and incompatible combinations in the graft callusing stage. Copyright © 2014 John Wiley & Sons, Ltd.</description><subject>benzoic acid</subject><subject>caffeic acid</subject><subject>catechin</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Cultivars</subject><subject>epicatechin</subject><subject>ferulic acid</subject><subject>flavonols</subject><subject>gallic acid</subject><subject>graft compatibility</subject><subject>graft incompatibility</subject><subject>high performance liquid chromatography</subject><subject>HPLC method</subject><subject>Liquid chromatography</subject><subject>metabolism</subject><subject>phenolic compounds</subject><subject>Phenols</subject><subject>Phenols - analysis</subject><subject>Phenols - isolation & purification</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - isolation & purification</subject><subject>quantitative analysis</subject><subject>rootstocks</subject><subject>sample extraction method</subject><subject>sinapic acid</subject><subject>tissues</subject><subject>Vitis</subject><subject>Vitis - chemistry</subject><subject>Vitis spp</subject><issn>0958-0344</issn><issn>1099-1565</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10VFv1SAYBuDGaNzZNPEXKIk33nQCpaV4d1ZnXTLnFt28JBxKd5gUKtCj52f4j6We40xMvCLhe3jDlzfLniF4jCDEr0cpjnGJqwfZAkHGclRW5cNsAVlZ57Ag5CA7DOEOwjRj1ePsAJO6rkuGF9nPy7WyzmgJGjeMbrJdAGd248xGdUBb0HrRR21v06VMQES90kbHLXA9uNFRBxDG8Q14qzbKuHFQNgJhO3AjjO4SdnaGwoKlFWYbtRQGfFBx7TrQOw_iWmkPriZho-7TcH7wJHvUCxPU0_15lF2_O_3cvM_PP7ZnzfI8l4SQKu-QZAXDiBKKZMUKzERPUE0wwRBjJehK1AoS2eFVzXpMBUMFgrIUFONqVdLiKHu1yx29-zapEPmgg1TGCKvcFDiqihJCUsMq0Zf_0Ds3-bTRb0Xmb2D0N1B6F4JXPR-9HoTfcgT5XBNPNfG5pkSf7wOn1aC6e_inlwTyHfiujdr-N4hfNst94N7rENWPey_8V17Rgpb8y0XLr5oT2tZtwS-Sf7HzvXBc3Hod-PUnDFHaGDGCKS5-Ac1Ts8c</recordid><startdate>201501</startdate><enddate>201501</enddate><creator>Canas, Sara</creator><creator>Assunção, Maria</creator><creator>Brazão, João</creator><creator>Zanol, Geni</creator><creator>Eiras‐Dias, José Eduardo</creator><general>Wiley</general><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201501</creationdate><title>Phenolic Compounds Involved in Grafting Incompatibility of Vitis spp: Development and Validation of an Analytical Method for their Quantification</title><author>Canas, Sara ; Assunção, Maria ; Brazão, João ; Zanol, Geni ; Eiras‐Dias, José Eduardo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4446-d1c939217471c69329af4184242022ea7ba8e04cd2b89f27a91310c5a7226b573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>benzoic acid</topic><topic>caffeic acid</topic><topic>catechin</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Cultivars</topic><topic>epicatechin</topic><topic>ferulic acid</topic><topic>flavonols</topic><topic>gallic acid</topic><topic>graft compatibility</topic><topic>graft incompatibility</topic><topic>high performance liquid chromatography</topic><topic>HPLC method</topic><topic>Liquid chromatography</topic><topic>metabolism</topic><topic>phenolic compounds</topic><topic>Phenols</topic><topic>Phenols - analysis</topic><topic>Phenols - isolation & purification</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - isolation & purification</topic><topic>quantitative analysis</topic><topic>rootstocks</topic><topic>sample extraction method</topic><topic>sinapic acid</topic><topic>tissues</topic><topic>Vitis</topic><topic>Vitis - chemistry</topic><topic>Vitis spp</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Canas, Sara</creatorcontrib><creatorcontrib>Assunção, Maria</creatorcontrib><creatorcontrib>Brazão, João</creatorcontrib><creatorcontrib>Zanol, Geni</creatorcontrib><creatorcontrib>Eiras‐Dias, José Eduardo</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Phytochemical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Canas, Sara</au><au>Assunção, Maria</au><au>Brazão, João</au><au>Zanol, Geni</au><au>Eiras‐Dias, José Eduardo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phenolic Compounds Involved in Grafting Incompatibility of Vitis spp: Development and Validation of an Analytical Method for their Quantification</atitle><jtitle>Phytochemical analysis</jtitle><addtitle>Phytochem. Anal</addtitle><date>2015-01</date><risdate>2015</risdate><volume>26</volume><issue>1</issue><spage>1</spage><epage>7</epage><pages>1-7</pages><issn>0958-0344</issn><eissn>1099-1565</eissn><coden>PHANEL</coden><abstract>INTRODUCTION: Graft incompatibility of Vitis spp is an unresolved worldwide problem with important economic consequences. Grafting comprises a complex set of morphological and physiological alterations, in which the phenolic compounds seem to be strongly involved. Therefore, a detailed analysis and recognition of structural phenolic compounds diversity in the two partners of a Vitis graft is of great importance to evaluate their role as markers of graft establishment. OBJECTIVE: To optimise a sample extraction method, and to develop and validate a high‐performance liquid chromatography (HPLC) method for the simultaneous determination of phenolic acids and flavonols in the graft union so as to understand their behaviour in the metabolism of the scion–rootstock system, using compatible and incompatible combinations of a Syrah cultivar and two rootstocks (R110 and SO4). METHODS: Sixty extracts of Vitis grafting tissues were prepared and analysed by HPLC for the qualitative and quantitative determination of their phenolic profile. RESULTS: Among the phenolic compounds identified in the samples, one benzoic acid (gallic acid), three cinnamic acids (caffeic acid, ferulic acid and sinapic acid) and two flavonols (catechin and epicatechin) are potentially suitable as markers of graft incompatibility. CONCLUSION: The method developed presents good performance and lends itself readily for application in routine analysis of the phenolic composition of Vitis grafting tissues to distinguish compatible and incompatible combinations in the graft callusing stage. Copyright © 2014 John Wiley & Sons, Ltd.</abstract><cop>England</cop><pub>Wiley</pub><pmid>24888592</pmid><doi>10.1002/pca.2526</doi><tpages>7</tpages></addata></record> |
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| subjects | benzoic acid caffeic acid catechin Chromatography, High Pressure Liquid - methods Cultivars epicatechin ferulic acid flavonols gallic acid graft compatibility graft incompatibility high performance liquid chromatography HPLC method Liquid chromatography metabolism phenolic compounds Phenols Phenols - analysis Phenols - isolation & purification Plant Extracts - chemistry Plant Extracts - isolation & purification quantitative analysis rootstocks sample extraction method sinapic acid tissues Vitis Vitis - chemistry Vitis spp |
| title | Phenolic Compounds Involved in Grafting Incompatibility of Vitis spp: Development and Validation of an Analytical Method for their Quantification |
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