Increased trophoblast expression of NFAT5/TonEBP in pre-eclamptic placentas and hyperosmolar-treated BeWo cells
Abstract Objectives To assess the concentrations of inositol and sorbitol, and determine the expression of related osmolyte factors [nuclear factor of activated T cells 5, also known as tonicity responsive binding protein (NFAT5/TonEBP); sodium myo-inositol transporter (SLC5A3); and aldose reductase...
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| Veröffentlicht in: | European journal of obstetrics & gynecology and reproductive biology 2014-12, Vol.183, p.37-43 |
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| container_title | European journal of obstetrics & gynecology and reproductive biology |
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| creator | Lee, G.T Price, M.D Mejia, C.A Galan, H.L Arroyo, J.A |
| description | Abstract Objectives To assess the concentrations of inositol and sorbitol, and determine the expression of related osmolyte factors [nuclear factor of activated T cells 5, also known as tonicity responsive binding protein (NFAT5/TonEBP); sodium myo-inositol transporter (SLC5A3); and aldose reductase] in placentas of pre-eclamptic (PE) patients and trophoblast BeWo cells subjected to hypertonic stress in vitro. Study design Control and PE placentas were collected. BeWo cells were cultured and subjected to a hyperosmolar solution for 4 h. Western blot analysis was performed on NFAT5, SLC5A3, aldose reductase and ERK proteins. High-performance liquid chromatography was used to determine the levels of inositol and sorbitol in cell lysates. Results Compared with control placentas, PE placentas showed higher levels of inositol and NFAT5, and lower levels of SLC5A3. Treated BeWo cells showed higher levels of inositol, sorbitol, NFAT5 total protein, SLC5A3 and aldose reductase, and increased ERK activation compared with control BeWo cells. Conclusions Hyperosmolar conditions increase the expression of NFAT5 in PE placentas and BeWo cells, and may account for the increased osmolyte levels. NFAT5 may accomplish this through aldose reductase and SLC5A3 in trophoblast cells. |
| doi_str_mv | 10.1016/j.ejogrb.2014.10.002 |
| format | Article |
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Study design Control and PE placentas were collected. BeWo cells were cultured and subjected to a hyperosmolar solution for 4 h. Western blot analysis was performed on NFAT5, SLC5A3, aldose reductase and ERK proteins. High-performance liquid chromatography was used to determine the levels of inositol and sorbitol in cell lysates. Results Compared with control placentas, PE placentas showed higher levels of inositol and NFAT5, and lower levels of SLC5A3. Treated BeWo cells showed higher levels of inositol, sorbitol, NFAT5 total protein, SLC5A3 and aldose reductase, and increased ERK activation compared with control BeWo cells. Conclusions Hyperosmolar conditions increase the expression of NFAT5 in PE placentas and BeWo cells, and may account for the increased osmolyte levels. NFAT5 may accomplish this through aldose reductase and SLC5A3 in trophoblast cells.</description><identifier>ISSN: 0301-2115</identifier><identifier>EISSN: 1872-7654</identifier><identifier>DOI: 10.1016/j.ejogrb.2014.10.002</identifier><identifier>PMID: 25461350</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Aldehyde Reductase - metabolism ; Case-Control Studies ; Cells, Cultured ; Choriocarcinoma - metabolism ; Choriocarcinoma - pathology ; Extracellular Signal-Regulated MAP Kinases - metabolism ; Female ; Heat-Shock Proteins - metabolism ; Humans ; In Vitro Techniques ; Inositol ; Inositol - metabolism ; NFAT5/TonEBP ; Obstetrics and Gynecology ; Osmolar Concentration ; Placenta - drug effects ; Placenta - metabolism ; Placenta - pathology ; Pre-eclampsia ; Pre-Eclampsia - metabolism ; Pre-Eclampsia - pathology ; Pregnancy ; Saline Solution, Hypertonic - pharmacology ; Sorbitol ; Sorbitol - metabolism ; Symporters - metabolism ; Transcription Factors - metabolism ; Trophoblast ; Trophoblasts - drug effects ; Trophoblasts - metabolism ; Trophoblasts - pathology ; Uterine Neoplasms - metabolism ; Uterine Neoplasms - pathology</subject><ispartof>European journal of obstetrics & gynecology and reproductive biology, 2014-12, Vol.183, p.37-43</ispartof><rights>Elsevier Ireland Ltd</rights><rights>2014 Elsevier Ireland Ltd</rights><rights>Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-19826c80825827e2da1e3a7c1cd172ec937bdebe97b376d3b6a3fa849bde4dfa3</citedby><cites>FETCH-LOGICAL-c417t-19826c80825827e2da1e3a7c1cd172ec937bdebe97b376d3b6a3fa849bde4dfa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ejogrb.2014.10.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25461350$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, G.T</creatorcontrib><creatorcontrib>Price, M.D</creatorcontrib><creatorcontrib>Mejia, C.A</creatorcontrib><creatorcontrib>Galan, H.L</creatorcontrib><creatorcontrib>Arroyo, J.A</creatorcontrib><title>Increased trophoblast expression of NFAT5/TonEBP in pre-eclamptic placentas and hyperosmolar-treated BeWo cells</title><title>European journal of obstetrics & gynecology and reproductive biology</title><addtitle>Eur J Obstet Gynecol Reprod Biol</addtitle><description>Abstract Objectives To assess the concentrations of inositol and sorbitol, and determine the expression of related osmolyte factors [nuclear factor of activated T cells 5, also known as tonicity responsive binding protein (NFAT5/TonEBP); sodium myo-inositol transporter (SLC5A3); and aldose reductase] in placentas of pre-eclamptic (PE) patients and trophoblast BeWo cells subjected to hypertonic stress in vitro. Study design Control and PE placentas were collected. BeWo cells were cultured and subjected to a hyperosmolar solution for 4 h. Western blot analysis was performed on NFAT5, SLC5A3, aldose reductase and ERK proteins. High-performance liquid chromatography was used to determine the levels of inositol and sorbitol in cell lysates. Results Compared with control placentas, PE placentas showed higher levels of inositol and NFAT5, and lower levels of SLC5A3. Treated BeWo cells showed higher levels of inositol, sorbitol, NFAT5 total protein, SLC5A3 and aldose reductase, and increased ERK activation compared with control BeWo cells. Conclusions Hyperosmolar conditions increase the expression of NFAT5 in PE placentas and BeWo cells, and may account for the increased osmolyte levels. NFAT5 may accomplish this through aldose reductase and SLC5A3 in trophoblast cells.</description><subject>Aldehyde Reductase - metabolism</subject><subject>Case-Control Studies</subject><subject>Cells, Cultured</subject><subject>Choriocarcinoma - metabolism</subject><subject>Choriocarcinoma - pathology</subject><subject>Extracellular Signal-Regulated MAP Kinases - metabolism</subject><subject>Female</subject><subject>Heat-Shock Proteins - metabolism</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Inositol</subject><subject>Inositol - metabolism</subject><subject>NFAT5/TonEBP</subject><subject>Obstetrics and Gynecology</subject><subject>Osmolar Concentration</subject><subject>Placenta - drug effects</subject><subject>Placenta - metabolism</subject><subject>Placenta - pathology</subject><subject>Pre-eclampsia</subject><subject>Pre-Eclampsia - metabolism</subject><subject>Pre-Eclampsia - pathology</subject><subject>Pregnancy</subject><subject>Saline Solution, Hypertonic - pharmacology</subject><subject>Sorbitol</subject><subject>Sorbitol - metabolism</subject><subject>Symporters - metabolism</subject><subject>Transcription Factors - metabolism</subject><subject>Trophoblast</subject><subject>Trophoblasts - drug effects</subject><subject>Trophoblasts - metabolism</subject><subject>Trophoblasts - pathology</subject><subject>Uterine Neoplasms - metabolism</subject><subject>Uterine Neoplasms - pathology</subject><issn>0301-2115</issn><issn>1872-7654</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1vFDEMhiMEotvCP0AoRy6zjZP5vCC1VUsrVYDEIo5RJvHQDDPJkGRR99-T0RYOXMglkv36tf2YkDfAtsCgPh-3OPrvod9yBmUObRnjz8gG2oYXTV2Vz8mGCQYFB6hOyGmMI8tPiO4lOeFVWYOo2Ib4O6cDqoiGpuCXB99PKiaKj0vAGK131A_0483FrjrfeXd9-ZlaR3OuQD2peUlW02VSGl1SkSpn6MNhweDj7CcVipStU7a-xG-eapym-Iq8GNQU8fXTf0a-3lzvrm6L-08f7q4u7gtdQpMK6Fpe65a1vGp5g9woQKEaDdpAw1F3oukN9tg1vWhqI_paiUG1ZZejpRmUOCPvjr5L8D_3GJOcbVwnUA79Pkqo8_qZhoAsLY9SneeOAQe5BDurcJDA5IpajvKIWq6o12hGncvePnXY9zOav0V_2GbB-6MA856_LAYZtUWn0diAOknj7f86_GugJ-usVtMPPGAc_T64zFCCjFwy-WU993ptKBmreM3Eb86jp1c</recordid><startdate>20141201</startdate><enddate>20141201</enddate><creator>Lee, G.T</creator><creator>Price, M.D</creator><creator>Mejia, C.A</creator><creator>Galan, H.L</creator><creator>Arroyo, J.A</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20141201</creationdate><title>Increased trophoblast expression of NFAT5/TonEBP in pre-eclamptic placentas and hyperosmolar-treated BeWo cells</title><author>Lee, G.T ; Price, M.D ; Mejia, C.A ; Galan, H.L ; Arroyo, J.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-19826c80825827e2da1e3a7c1cd172ec937bdebe97b376d3b6a3fa849bde4dfa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Aldehyde Reductase - metabolism</topic><topic>Case-Control Studies</topic><topic>Cells, Cultured</topic><topic>Choriocarcinoma - metabolism</topic><topic>Choriocarcinoma - pathology</topic><topic>Extracellular Signal-Regulated MAP Kinases - metabolism</topic><topic>Female</topic><topic>Heat-Shock Proteins - metabolism</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Inositol</topic><topic>Inositol - metabolism</topic><topic>NFAT5/TonEBP</topic><topic>Obstetrics and Gynecology</topic><topic>Osmolar Concentration</topic><topic>Placenta - drug effects</topic><topic>Placenta - metabolism</topic><topic>Placenta - pathology</topic><topic>Pre-eclampsia</topic><topic>Pre-Eclampsia - metabolism</topic><topic>Pre-Eclampsia - pathology</topic><topic>Pregnancy</topic><topic>Saline Solution, Hypertonic - pharmacology</topic><topic>Sorbitol</topic><topic>Sorbitol - metabolism</topic><topic>Symporters - metabolism</topic><topic>Transcription Factors - metabolism</topic><topic>Trophoblast</topic><topic>Trophoblasts - drug effects</topic><topic>Trophoblasts - metabolism</topic><topic>Trophoblasts - pathology</topic><topic>Uterine Neoplasms - metabolism</topic><topic>Uterine Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, G.T</creatorcontrib><creatorcontrib>Price, M.D</creatorcontrib><creatorcontrib>Mejia, C.A</creatorcontrib><creatorcontrib>Galan, H.L</creatorcontrib><creatorcontrib>Arroyo, J.A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of obstetrics & gynecology and reproductive biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, G.T</au><au>Price, M.D</au><au>Mejia, C.A</au><au>Galan, H.L</au><au>Arroyo, J.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased trophoblast expression of NFAT5/TonEBP in pre-eclamptic placentas and hyperosmolar-treated BeWo cells</atitle><jtitle>European journal of obstetrics & gynecology and reproductive biology</jtitle><addtitle>Eur J Obstet Gynecol Reprod Biol</addtitle><date>2014-12-01</date><risdate>2014</risdate><volume>183</volume><spage>37</spage><epage>43</epage><pages>37-43</pages><issn>0301-2115</issn><eissn>1872-7654</eissn><abstract>Abstract Objectives To assess the concentrations of inositol and sorbitol, and determine the expression of related osmolyte factors [nuclear factor of activated T cells 5, also known as tonicity responsive binding protein (NFAT5/TonEBP); sodium myo-inositol transporter (SLC5A3); and aldose reductase] in placentas of pre-eclamptic (PE) patients and trophoblast BeWo cells subjected to hypertonic stress in vitro. Study design Control and PE placentas were collected. BeWo cells were cultured and subjected to a hyperosmolar solution for 4 h. Western blot analysis was performed on NFAT5, SLC5A3, aldose reductase and ERK proteins. High-performance liquid chromatography was used to determine the levels of inositol and sorbitol in cell lysates. Results Compared with control placentas, PE placentas showed higher levels of inositol and NFAT5, and lower levels of SLC5A3. Treated BeWo cells showed higher levels of inositol, sorbitol, NFAT5 total protein, SLC5A3 and aldose reductase, and increased ERK activation compared with control BeWo cells. Conclusions Hyperosmolar conditions increase the expression of NFAT5 in PE placentas and BeWo cells, and may account for the increased osmolyte levels. NFAT5 may accomplish this through aldose reductase and SLC5A3 in trophoblast cells.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>25461350</pmid><doi>10.1016/j.ejogrb.2014.10.002</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0301-2115 |
| ispartof | European journal of obstetrics & gynecology and reproductive biology, 2014-12, Vol.183, p.37-43 |
| issn | 0301-2115 1872-7654 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Aldehyde Reductase - metabolism Case-Control Studies Cells, Cultured Choriocarcinoma - metabolism Choriocarcinoma - pathology Extracellular Signal-Regulated MAP Kinases - metabolism Female Heat-Shock Proteins - metabolism Humans In Vitro Techniques Inositol Inositol - metabolism NFAT5/TonEBP Obstetrics and Gynecology Osmolar Concentration Placenta - drug effects Placenta - metabolism Placenta - pathology Pre-eclampsia Pre-Eclampsia - metabolism Pre-Eclampsia - pathology Pregnancy Saline Solution, Hypertonic - pharmacology Sorbitol Sorbitol - metabolism Symporters - metabolism Transcription Factors - metabolism Trophoblast Trophoblasts - drug effects Trophoblasts - metabolism Trophoblasts - pathology Uterine Neoplasms - metabolism Uterine Neoplasms - pathology |
| title | Increased trophoblast expression of NFAT5/TonEBP in pre-eclamptic placentas and hyperosmolar-treated BeWo cells |
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