Mutants of human insulin-like growth factor II (IGF II) : expression and characterization of truncated IGF II and of two naturally occurring variants

Insulin-like growth factor II (IGF II) and four structural analogs, constructed by site-directed mutagenesis, were expressed as protein A fusion proteins in Escherichia coli BL21pLysS cells, cleaved with cyanogen bromide and purified by affinity chromatography and HPLC. Two mutants (Ser29 substitute...

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Veröffentlicht in:	European journal of biochemistry 1992, Vol.205 (2), p.483-490
Hauptverfasser:	LÜTHI, C, ROTH, B. V, HUMBEL, R. E
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical, structural and metabolic biochemistry Animals Base Sequence Binding, Competitive biochemical characteristics Biological and medical sciences Carrier Proteins - metabolism Cell Line Cloning, Molecular DNA - genetics DNA Replication - drug effects Fundamental and applied biological sciences. Psychology Humans Insulin-Like Growth Factor Binding Proteins insulin-like growth factor II Insulin-Like Growth Factor II - genetics Insulin-Like Growth Factor II - metabolism Insulin-Like Growth Factor II - pharmacology Intermediary metabolites. Miscellaneous Kinetics man Mannosephosphates - metabolism Molecular Sequence Data Mutagenesis, Site-Directed Oligodeoxyribonucleotides Other biological molecules Receptor, IGF Type 2 Receptors, Cell Surface - metabolism Recombinant Proteins - metabolism Recombinant Proteins - pharmacology Restriction Mapping serine site-directed mutagenesis Thymidine - metabolism
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