Determining tissue origin of circulating epithelial cells (CEC) in patients with differentiated thyroid cancer by real-time PCR using thyroid mRNA probes
Abstract The aim of this study is to determine whether circulating epithelial cells (CEC) detected in patients with differentiated thyroid cancer (DTC) stem from the thyroid gland. CEC have been described to increase in patients with progressive cancer disease and thus have been used as a marker of...
Gespeichert in:
| Veröffentlicht in: | Cancer letters 2015-01, Vol.356 (2), p.491-495 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 495 |
|---|---|
| container_issue | 2 |
| container_start_page | 491 |
| container_title | Cancer letters |
| container_volume | 356 |
| creator | Sorg, Sarah Pachmann, Katharina Brede-Hekimian, Katya Freesmeyer, Martin Winkens, Thomas |
| description | Abstract The aim of this study is to determine whether circulating epithelial cells (CEC) detected in patients with differentiated thyroid cancer (DTC) stem from the thyroid gland. CEC have been described to increase in patients with progressive cancer disease and thus have been used as a marker of tumour cell dissemination. CEC were selected from venous blood samples of five DTC patients and analysis of thyroid-specific mRNA (i.e. Tg, TSH-R, TPO, NIS) was performed on a single cell level. 16/48 cells were positive for at least three different thyroid-mRNA transcripts, predominantly found in patients with detectable serum thyroglobulin. In conclusion, evidence was found that in patients with detectable serum thyroglobulin, most of the CECs originate from the thyroid gland. However, further investigations including a larger sample size are needed to validate the clinical impact of this method. |
| doi_str_mv | 10.1016/j.canlet.2014.09.046 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1634722578</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S0304383514005874</els_id><sourcerecordid>3515913431</sourcerecordid><originalsourceid>FETCH-LOGICAL-c445t-e1cfd072b2f2de151415aaa64346ac011a305ebc4cc9d13cbb5bfb2df76eb6223</originalsourceid><addsrcrecordid>eNqFkstu1DAUhi0EokPhDRCyxKYsEnzNZYNUhXKRKkAF1pbtnLQenGRqO6B5FN4WhxlA6oaVZZ3vXP8foaeUlJTQ6uW2tHrykEpGqChJWxJR3UMb2tSsqNuG3EcbwokoeMPlCXoU45YQIkUtH6ITJnOE12yDfr6GBGF0k5uucXIxLoDn4K7dhOcBWxfs4nVag7Bz6Qa80x5b8D7is-6ie4EzuMsATCniH5nAvRsGCPnvdIIep5t9mF2P87AWAjZ7HED7IrkR8KfuCi_xd-cjNV59OMe7MBuIj9GDQfsIT47vKfr65uJL9664_Pj2fXd-WVghZCqA2qEnNTNsYD1QSQWVWutKcFFpSyjVnEgwVljb9pRbY6QZDOuHugJTMcZP0dmhbm57u0BManRx3VBPMC9R0YqLmjFZNxl9fgfdzkuY8nSZYm3byqpaKXGgbJhjDDCoXXCjDntFiVqlU1t1kE6t0inSqixdTnt2LL6YEfq_SX-0ysCrAwD5Gt8dBBVtvruF3gWwSfWz-1-HuwWsz8Jb7b_BHuK_XVRkiqjPq31W91CRjdPUgv8CJKvDEQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1629995668</pqid></control><display><type>article</type><title>Determining tissue origin of circulating epithelial cells (CEC) in patients with differentiated thyroid cancer by real-time PCR using thyroid mRNA probes</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Sorg, Sarah ; Pachmann, Katharina ; Brede-Hekimian, Katya ; Freesmeyer, Martin ; Winkens, Thomas</creator><creatorcontrib>Sorg, Sarah ; Pachmann, Katharina ; Brede-Hekimian, Katya ; Freesmeyer, Martin ; Winkens, Thomas</creatorcontrib><description>Abstract The aim of this study is to determine whether circulating epithelial cells (CEC) detected in patients with differentiated thyroid cancer (DTC) stem from the thyroid gland. CEC have been described to increase in patients with progressive cancer disease and thus have been used as a marker of tumour cell dissemination. CEC were selected from venous blood samples of five DTC patients and analysis of thyroid-specific mRNA (i.e. Tg, TSH-R, TPO, NIS) was performed on a single cell level. 16/48 cells were positive for at least three different thyroid-mRNA transcripts, predominantly found in patients with detectable serum thyroglobulin. In conclusion, evidence was found that in patients with detectable serum thyroglobulin, most of the CECs originate from the thyroid gland. However, further investigations including a larger sample size are needed to validate the clinical impact of this method.</description><identifier>ISSN: 0304-3835</identifier><identifier>EISSN: 1872-7980</identifier><identifier>DOI: 10.1016/j.canlet.2014.09.046</identifier><identifier>PMID: 25304372</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Antigens, Neoplasm - genetics ; Antigens, Neoplasm - metabolism ; Autoantigens - genetics ; Autoantigens - metabolism ; Binding sites ; Biomarkers ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Cell Adhesion Molecules - genetics ; Cell Adhesion Molecules - metabolism ; Cell Differentiation ; Circulating tumour cells ; CTC ; EpCAM ; Epithelial Cell Adhesion Molecule ; Epithelial Cells - metabolism ; Epithelial Cells - pathology ; Hematology, Oncology and Palliative Medicine ; Hormones ; Humans ; Iodide Peroxidase - genetics ; Iodide Peroxidase - metabolism ; Iron-Binding Proteins - genetics ; Iron-Binding Proteins - metabolism ; Medical prognosis ; Metastasis ; mRNA ; Neoplastic Cells, Circulating - metabolism ; Neoplastic Cells, Circulating - pathology ; Patients ; Real-Time Polymerase Chain Reaction ; Receptors, Thyrotropin - genetics ; Receptors, Thyrotropin - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; Studies ; Symporters - genetics ; Symporters - metabolism ; Thyroid cancer ; Thyroid Neoplasms - genetics ; Thyroid Neoplasms - pathology ; TSH-R ; Tumor Cells, Cultured</subject><ispartof>Cancer letters, 2015-01, Vol.356 (2), p.491-495</ispartof><rights>Elsevier Ireland Ltd</rights><rights>2014 Elsevier Ireland Ltd</rights><rights>Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.</rights><rights>Copyright Elsevier Limited Jan 28, 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-e1cfd072b2f2de151415aaa64346ac011a305ebc4cc9d13cbb5bfb2df76eb6223</citedby><cites>FETCH-LOGICAL-c445t-e1cfd072b2f2de151415aaa64346ac011a305ebc4cc9d13cbb5bfb2df76eb6223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.canlet.2014.09.046$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25304372$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sorg, Sarah</creatorcontrib><creatorcontrib>Pachmann, Katharina</creatorcontrib><creatorcontrib>Brede-Hekimian, Katya</creatorcontrib><creatorcontrib>Freesmeyer, Martin</creatorcontrib><creatorcontrib>Winkens, Thomas</creatorcontrib><title>Determining tissue origin of circulating epithelial cells (CEC) in patients with differentiated thyroid cancer by real-time PCR using thyroid mRNA probes</title><title>Cancer letters</title><addtitle>Cancer Lett</addtitle><description>Abstract The aim of this study is to determine whether circulating epithelial cells (CEC) detected in patients with differentiated thyroid cancer (DTC) stem from the thyroid gland. CEC have been described to increase in patients with progressive cancer disease and thus have been used as a marker of tumour cell dissemination. CEC were selected from venous blood samples of five DTC patients and analysis of thyroid-specific mRNA (i.e. Tg, TSH-R, TPO, NIS) was performed on a single cell level. 16/48 cells were positive for at least three different thyroid-mRNA transcripts, predominantly found in patients with detectable serum thyroglobulin. In conclusion, evidence was found that in patients with detectable serum thyroglobulin, most of the CECs originate from the thyroid gland. However, further investigations including a larger sample size are needed to validate the clinical impact of this method.</description><subject>Antigens, Neoplasm - genetics</subject><subject>Antigens, Neoplasm - metabolism</subject><subject>Autoantigens - genetics</subject><subject>Autoantigens - metabolism</subject><subject>Binding sites</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Cell Adhesion Molecules - genetics</subject><subject>Cell Adhesion Molecules - metabolism</subject><subject>Cell Differentiation</subject><subject>Circulating tumour cells</subject><subject>CTC</subject><subject>EpCAM</subject><subject>Epithelial Cell Adhesion Molecule</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelial Cells - pathology</subject><subject>Hematology, Oncology and Palliative Medicine</subject><subject>Hormones</subject><subject>Humans</subject><subject>Iodide Peroxidase - genetics</subject><subject>Iodide Peroxidase - metabolism</subject><subject>Iron-Binding Proteins - genetics</subject><subject>Iron-Binding Proteins - metabolism</subject><subject>Medical prognosis</subject><subject>Metastasis</subject><subject>mRNA</subject><subject>Neoplastic Cells, Circulating - metabolism</subject><subject>Neoplastic Cells, Circulating - pathology</subject><subject>Patients</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Receptors, Thyrotropin - genetics</subject><subject>Receptors, Thyrotropin - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>Studies</subject><subject>Symporters - genetics</subject><subject>Symporters - metabolism</subject><subject>Thyroid cancer</subject><subject>Thyroid Neoplasms - genetics</subject><subject>Thyroid Neoplasms - pathology</subject><subject>TSH-R</subject><subject>Tumor Cells, Cultured</subject><issn>0304-3835</issn><issn>1872-7980</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkstu1DAUhi0EokPhDRCyxKYsEnzNZYNUhXKRKkAF1pbtnLQenGRqO6B5FN4WhxlA6oaVZZ3vXP8foaeUlJTQ6uW2tHrykEpGqChJWxJR3UMb2tSsqNuG3EcbwokoeMPlCXoU45YQIkUtH6ITJnOE12yDfr6GBGF0k5uucXIxLoDn4K7dhOcBWxfs4nVag7Bz6Qa80x5b8D7is-6ie4EzuMsATCniH5nAvRsGCPnvdIIep5t9mF2P87AWAjZ7HED7IrkR8KfuCi_xd-cjNV59OMe7MBuIj9GDQfsIT47vKfr65uJL9664_Pj2fXd-WVghZCqA2qEnNTNsYD1QSQWVWutKcFFpSyjVnEgwVljb9pRbY6QZDOuHugJTMcZP0dmhbm57u0BManRx3VBPMC9R0YqLmjFZNxl9fgfdzkuY8nSZYm3byqpaKXGgbJhjDDCoXXCjDntFiVqlU1t1kE6t0inSqixdTnt2LL6YEfq_SX-0ysCrAwD5Gt8dBBVtvruF3gWwSfWz-1-HuwWsz8Jb7b_BHuK_XVRkiqjPq31W91CRjdPUgv8CJKvDEQ</recordid><startdate>20150128</startdate><enddate>20150128</enddate><creator>Sorg, Sarah</creator><creator>Pachmann, Katharina</creator><creator>Brede-Hekimian, Katya</creator><creator>Freesmeyer, Martin</creator><creator>Winkens, Thomas</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>20150128</creationdate><title>Determining tissue origin of circulating epithelial cells (CEC) in patients with differentiated thyroid cancer by real-time PCR using thyroid mRNA probes</title><author>Sorg, Sarah ; Pachmann, Katharina ; Brede-Hekimian, Katya ; Freesmeyer, Martin ; Winkens, Thomas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-e1cfd072b2f2de151415aaa64346ac011a305ebc4cc9d13cbb5bfb2df76eb6223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Antigens, Neoplasm - genetics</topic><topic>Antigens, Neoplasm - metabolism</topic><topic>Autoantigens - genetics</topic><topic>Autoantigens - metabolism</topic><topic>Binding sites</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Cell Adhesion Molecules - genetics</topic><topic>Cell Adhesion Molecules - metabolism</topic><topic>Cell Differentiation</topic><topic>Circulating tumour cells</topic><topic>CTC</topic><topic>EpCAM</topic><topic>Epithelial Cell Adhesion Molecule</topic><topic>Epithelial Cells - metabolism</topic><topic>Epithelial Cells - pathology</topic><topic>Hematology, Oncology and Palliative Medicine</topic><topic>Hormones</topic><topic>Humans</topic><topic>Iodide Peroxidase - genetics</topic><topic>Iodide Peroxidase - metabolism</topic><topic>Iron-Binding Proteins - genetics</topic><topic>Iron-Binding Proteins - metabolism</topic><topic>Medical prognosis</topic><topic>Metastasis</topic><topic>mRNA</topic><topic>Neoplastic Cells, Circulating - metabolism</topic><topic>Neoplastic Cells, Circulating - pathology</topic><topic>Patients</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Receptors, Thyrotropin - genetics</topic><topic>Receptors, Thyrotropin - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>Studies</topic><topic>Symporters - genetics</topic><topic>Symporters - metabolism</topic><topic>Thyroid cancer</topic><topic>Thyroid Neoplasms - genetics</topic><topic>Thyroid Neoplasms - pathology</topic><topic>TSH-R</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sorg, Sarah</creatorcontrib><creatorcontrib>Pachmann, Katharina</creatorcontrib><creatorcontrib>Brede-Hekimian, Katya</creatorcontrib><creatorcontrib>Freesmeyer, Martin</creatorcontrib><creatorcontrib>Winkens, Thomas</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sorg, Sarah</au><au>Pachmann, Katharina</au><au>Brede-Hekimian, Katya</au><au>Freesmeyer, Martin</au><au>Winkens, Thomas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determining tissue origin of circulating epithelial cells (CEC) in patients with differentiated thyroid cancer by real-time PCR using thyroid mRNA probes</atitle><jtitle>Cancer letters</jtitle><addtitle>Cancer Lett</addtitle><date>2015-01-28</date><risdate>2015</risdate><volume>356</volume><issue>2</issue><spage>491</spage><epage>495</epage><pages>491-495</pages><issn>0304-3835</issn><eissn>1872-7980</eissn><abstract>Abstract The aim of this study is to determine whether circulating epithelial cells (CEC) detected in patients with differentiated thyroid cancer (DTC) stem from the thyroid gland. CEC have been described to increase in patients with progressive cancer disease and thus have been used as a marker of tumour cell dissemination. CEC were selected from venous blood samples of five DTC patients and analysis of thyroid-specific mRNA (i.e. Tg, TSH-R, TPO, NIS) was performed on a single cell level. 16/48 cells were positive for at least three different thyroid-mRNA transcripts, predominantly found in patients with detectable serum thyroglobulin. In conclusion, evidence was found that in patients with detectable serum thyroglobulin, most of the CECs originate from the thyroid gland. However, further investigations including a larger sample size are needed to validate the clinical impact of this method.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>25304372</pmid><doi>10.1016/j.canlet.2014.09.046</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-3835 |
| ispartof | Cancer letters, 2015-01, Vol.356 (2), p.491-495 |
| issn | 0304-3835 1872-7980 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1634722578 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Antigens, Neoplasm - genetics Antigens, Neoplasm - metabolism Autoantigens - genetics Autoantigens - metabolism Binding sites Biomarkers Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Cell Adhesion Molecules - genetics Cell Adhesion Molecules - metabolism Cell Differentiation Circulating tumour cells CTC EpCAM Epithelial Cell Adhesion Molecule Epithelial Cells - metabolism Epithelial Cells - pathology Hematology, Oncology and Palliative Medicine Hormones Humans Iodide Peroxidase - genetics Iodide Peroxidase - metabolism Iron-Binding Proteins - genetics Iron-Binding Proteins - metabolism Medical prognosis Metastasis mRNA Neoplastic Cells, Circulating - metabolism Neoplastic Cells, Circulating - pathology Patients Real-Time Polymerase Chain Reaction Receptors, Thyrotropin - genetics Receptors, Thyrotropin - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics Studies Symporters - genetics Symporters - metabolism Thyroid cancer Thyroid Neoplasms - genetics Thyroid Neoplasms - pathology TSH-R Tumor Cells, Cultured |
| title | Determining tissue origin of circulating epithelial cells (CEC) in patients with differentiated thyroid cancer by real-time PCR using thyroid mRNA probes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T13%3A59%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Determining%20tissue%20origin%20of%20circulating%20epithelial%20cells%20(CEC)%20in%20patients%20with%20differentiated%20thyroid%20cancer%20by%20real-time%20PCR%20using%20thyroid%20mRNA%20probes&rft.jtitle=Cancer%20letters&rft.au=Sorg,%20Sarah&rft.date=2015-01-28&rft.volume=356&rft.issue=2&rft.spage=491&rft.epage=495&rft.pages=491-495&rft.issn=0304-3835&rft.eissn=1872-7980&rft_id=info:doi/10.1016/j.canlet.2014.09.046&rft_dat=%3Cproquest_cross%3E3515913431%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1629995668&rft_id=info:pmid/25304372&rft_els_id=1_s2_0_S0304383514005874&rfr_iscdi=true |