Transposon mutagenesis in Staphylococcus epidermidis using the Enterococcus faecalis transposon Tn917
: We transformed a clinical Staphylococcus epidermidis isolate with the Enterococcus faecalis transposon Tn917‐carrying plasmid pTV1. Loss of plasmid replication was observed at 47°C. Tn917 transposes efficiently and apparently randomly. The transposition frequency could be stimulated by erythromyci...
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| Veröffentlicht in: | FEMS microbiology letters 1991-08, Vol.82 (2), p.215-218 |
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| container_title | FEMS microbiology letters |
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| creator | Grueter, Ludwig Koenig, Oliver Laufs, Rainer |
| description | : We transformed a clinical Staphylococcus epidermidis isolate with the Enterococcus faecalis transposon Tn917‐carrying plasmid pTV1. Loss of plasmid replication was observed at 47°C. Tn917 transposes efficiently and apparently randomly. The transposition frequency could be stimulated by erythromycin. Transposon mutagenesis in S. epidermidis provides a means for genetic study of the various virulence factors of this pathogen. |
| doi_str_mv | 10.1111/j.1574-6968.1991.tb04867.x |
| format | Article |
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Psychology</subject><subject>Genetics</subject><subject>Microbiology</subject><subject>Staphylococcus epidermidis</subject><subject>Tn917</subject><subject>Transposon mutagenesis</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNpFkF1LwzAUhoMoOKf_oYh415o0X82NIGNTYeKF8zqkabpltGltWtz-_VI257kJh_fh5OUB4B7BBIV52iaIchIzwbIECYGSPockYzzZXYDJOboEE4h5FiMo-DW48X4LISQpZBNgVp1yvm1846J66NXaOOOtj6yLvnrVbvZVoxutBx-Z1hamq20R0sFbt476jYnmrjfdH1Iqo1UV8v7_6MoJxG_BVakqb-5O7xR8L-ar2Vu8_Hx9n70s4zalmMahO8KlwoRiQRghaa5LxhGFeWoUKTAudfiCFgWFihKI04yGneWsRFRzKPAUPB7vtl3zMxjfy9p6bapKOdMMXiKGCaMpCuDDCVQ-VC5DX229bDtbq24viRCcZiP2fMR-bWX25xhBOdqXWzkqlqNiOdqXJ_tyJxcfyxRRfADuSn1w</recordid><startdate>199108</startdate><enddate>199108</enddate><creator>Grueter, Ludwig</creator><creator>Koenig, Oliver</creator><creator>Laufs, Rainer</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>199108</creationdate><title>Transposon mutagenesis in Staphylococcus epidermidis using the Enterococcus faecalis transposon Tn917</title><author>Grueter, Ludwig ; Koenig, Oliver ; Laufs, Rainer</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p2535-b0413fa3453946442bcf67150b2ea4d33fcfae5dd50a5403285fae6b6f15c7093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Enterococcus faecalis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>Microbiology</topic><topic>Staphylococcus epidermidis</topic><topic>Tn917</topic><topic>Transposon mutagenesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grueter, Ludwig</creatorcontrib><creatorcontrib>Koenig, Oliver</creatorcontrib><creatorcontrib>Laufs, Rainer</creatorcontrib><collection>Pascal-Francis</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grueter, Ludwig</au><au>Koenig, Oliver</au><au>Laufs, Rainer</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transposon mutagenesis in Staphylococcus epidermidis using the Enterococcus faecalis transposon Tn917</atitle><jtitle>FEMS microbiology letters</jtitle><date>1991-08</date><risdate>1991</risdate><volume>82</volume><issue>2</issue><spage>215</spage><epage>218</epage><pages>215-218</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>: We transformed a clinical Staphylococcus epidermidis isolate with the Enterococcus faecalis transposon Tn917‐carrying plasmid pTV1. 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| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1991-08, Vol.82 (2), p.215-218 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16346521 |
| source | Oxford University Press Journals Digital Archive legacy; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Bacteriology Biological and medical sciences Enterococcus faecalis Fundamental and applied biological sciences. Psychology Genetics Microbiology Staphylococcus epidermidis Tn917 Transposon mutagenesis |
| title | Transposon mutagenesis in Staphylococcus epidermidis using the Enterococcus faecalis transposon Tn917 |
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