Molecular analysis of an essential gene upstream of rpoN in Rhizobium NGR234
Rhizobium sp. NGR234 is a broad-host range strain. The rpoN gene of this organism encodes a sigma factor which is a primary co-regulator of endosymbiosis [1]. We characterized the locus upstream of rpoN, and identified a contiguous open reading frame, here termed ORF1. DNA sequence analysis of this...
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| Veröffentlicht in: | FEMS microbiology letters 1991-08, Vol.82 (2), p.195-201 |
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| creator | Slooten, J.C. van Stanley, J |
| description | Rhizobium sp. NGR234 is a broad-host range strain. The rpoN gene of this organism encodes a sigma factor which is a primary co-regulator of endosymbiosis [1]. We characterized the locus upstream of rpoN, and identified a contiguous open reading frame, here termed ORF1. DNA sequence analysis of this ORF showed that it encoded a polypeptide highly conserved with a corresponding ORF of Rhizobium meliloti. The gene product contained two ATP/GTP binding pockets. Codon usage in the ORF and the nitrogenase operon nifKDH of NGR234 was similar. Although we used a non-transposable cassette flanked by appropriate sized DNA fragments, we were unable to isolate site-directed mutants in the ORF, whose ATP/GTP binding protein product is thus probably of essential biological function. ORF1 and rpoN exhibited conserved linkage among diverse rhizobia, and in Azotobacter vinelandii. Intragenomic and interspecific homology studies confirmed directly that ORF1 (NGR234) belonged to a large family of ATP-binding protein genes. |
| doi_str_mv | 10.1111/j.1574-6968.1991.tb04864.x |
| format | Article |
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NGR234 is a broad-host range strain. The rpoN gene of this organism encodes a sigma factor which is a primary co-regulator of endosymbiosis [1]. We characterized the locus upstream of rpoN, and identified a contiguous open reading frame, here termed ORF1. DNA sequence analysis of this ORF showed that it encoded a polypeptide highly conserved with a corresponding ORF of Rhizobium meliloti. The gene product contained two ATP/GTP binding pockets. Codon usage in the ORF and the nitrogenase operon nifKDH of NGR234 was similar. Although we used a non-transposable cassette flanked by appropriate sized DNA fragments, we were unable to isolate site-directed mutants in the ORF, whose ATP/GTP binding protein product is thus probably of essential biological function. ORF1 and rpoN exhibited conserved linkage among diverse rhizobia, and in Azotobacter vinelandii. Intragenomic and interspecific homology studies confirmed directly that ORF1 (NGR234) belonged to a large family of ATP-binding protein genes.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1991.tb04864.x</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>amino acid sequences ; Bacteriology ; Biological and medical sciences ; Conserved ; Diazotroph ; DNA sequence ; Fundamental and applied biological sciences. Psychology ; genes ; genetic code ; genetic techniques and protocols ; Genetics ; loci ; Microbiology ; molecular genetics ; molecular sequence data ; nucleotide sequences ; open reading frames ; ORF1 ; Rhizobium ; Rhizobium meliloti ; sequence homology</subject><ispartof>FEMS microbiology letters, 1991-08, Vol.82 (2), p.195-201</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2475-e929b19736b6495bc3fb1dd6fb2ecaa36def26c5079e44f8a316f204f6fab68a3</citedby><cites>FETCH-LOGICAL-c2475-e929b19736b6495bc3fb1dd6fb2ecaa36def26c5079e44f8a316f204f6fab68a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.1991.tb04864.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.1991.tb04864.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5157172$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Slooten, J.C. van</creatorcontrib><creatorcontrib>Stanley, J</creatorcontrib><title>Molecular analysis of an essential gene upstream of rpoN in Rhizobium NGR234</title><title>FEMS microbiology letters</title><description>Rhizobium sp. NGR234 is a broad-host range strain. The rpoN gene of this organism encodes a sigma factor which is a primary co-regulator of endosymbiosis [1]. We characterized the locus upstream of rpoN, and identified a contiguous open reading frame, here termed ORF1. DNA sequence analysis of this ORF showed that it encoded a polypeptide highly conserved with a corresponding ORF of Rhizobium meliloti. The gene product contained two ATP/GTP binding pockets. Codon usage in the ORF and the nitrogenase operon nifKDH of NGR234 was similar. Although we used a non-transposable cassette flanked by appropriate sized DNA fragments, we were unable to isolate site-directed mutants in the ORF, whose ATP/GTP binding protein product is thus probably of essential biological function. ORF1 and rpoN exhibited conserved linkage among diverse rhizobia, and in Azotobacter vinelandii. Intragenomic and interspecific homology studies confirmed directly that ORF1 (NGR234) belonged to a large family of ATP-binding protein genes.</description><subject>amino acid sequences</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Conserved</subject><subject>Diazotroph</subject><subject>DNA sequence</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>genetic code</subject><subject>genetic techniques and protocols</subject><subject>Genetics</subject><subject>loci</subject><subject>Microbiology</subject><subject>molecular genetics</subject><subject>molecular sequence data</subject><subject>nucleotide sequences</subject><subject>open reading frames</subject><subject>ORF1</subject><subject>Rhizobium</subject><subject>Rhizobium meliloti</subject><subject>sequence homology</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNqVkFtLAzEQhYMoWC-_wUXEt11z2-zGB0HEG7QKXp7DbJpoSrpbky5af71ZWvruvMwMc-Yc-BA6JbggqS5mBSkrngsp6oJISYplg3ktePGzg0bb0y4aYVbVOcGy2kcHMc4wxpxiMULjSeeN7j2EDFrwq-hi1tk0ZyZG0y4d-OzDtCbrF3EZDMyHa1h0T5lrs5dP99s1rp9nT_cvlPEjtGfBR3O86Yfo_e727eYhHz_fP95cj3NNeVXmRlLZEFkx0Qguy0Yz25DpVNiGGg3AxNRYKnSJK2k4tzUwIizF3AoLjUjrITpf-y5C99WbuFRzF7XxHlrT9VERwXjJuUjCy7VQhy7GYKxaBDeHsFIEqwGgmqmBkhooqQGg2gBUP-n5bJMCUYO3AVrt4tahTI-kokl2tZZ9O29W_whQd5MxkWUyOFkbWOgUfISU8f5KMWGYVJzjumZ_XcmOMw</recordid><startdate>199108</startdate><enddate>199108</enddate><creator>Slooten, J.C. van</creator><creator>Stanley, J</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>199108</creationdate><title>Molecular analysis of an essential gene upstream of rpoN in Rhizobium NGR234</title><author>Slooten, J.C. van ; Stanley, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2475-e929b19736b6495bc3fb1dd6fb2ecaa36def26c5079e44f8a316f204f6fab68a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>amino acid sequences</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Conserved</topic><topic>Diazotroph</topic><topic>DNA sequence</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>genetic code</topic><topic>genetic techniques and protocols</topic><topic>Genetics</topic><topic>loci</topic><topic>Microbiology</topic><topic>molecular genetics</topic><topic>molecular sequence data</topic><topic>nucleotide sequences</topic><topic>open reading frames</topic><topic>ORF1</topic><topic>Rhizobium</topic><topic>Rhizobium meliloti</topic><topic>sequence homology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Slooten, J.C. van</creatorcontrib><creatorcontrib>Stanley, J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Slooten, J.C. van</au><au>Stanley, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular analysis of an essential gene upstream of rpoN in Rhizobium NGR234</atitle><jtitle>FEMS microbiology letters</jtitle><date>1991-08</date><risdate>1991</risdate><volume>82</volume><issue>2</issue><spage>195</spage><epage>201</epage><pages>195-201</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Rhizobium sp. NGR234 is a broad-host range strain. The rpoN gene of this organism encodes a sigma factor which is a primary co-regulator of endosymbiosis [1]. We characterized the locus upstream of rpoN, and identified a contiguous open reading frame, here termed ORF1. DNA sequence analysis of this ORF showed that it encoded a polypeptide highly conserved with a corresponding ORF of Rhizobium meliloti. The gene product contained two ATP/GTP binding pockets. Codon usage in the ORF and the nitrogenase operon nifKDH of NGR234 was similar. Although we used a non-transposable cassette flanked by appropriate sized DNA fragments, we were unable to isolate site-directed mutants in the ORF, whose ATP/GTP binding protein product is thus probably of essential biological function. ORF1 and rpoN exhibited conserved linkage among diverse rhizobia, and in Azotobacter vinelandii. Intragenomic and interspecific homology studies confirmed directly that ORF1 (NGR234) belonged to a large family of ATP-binding protein genes.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1574-6968.1991.tb04864.x</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1991-08, Vol.82 (2), p.195-201 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16345446 |
| source | Wiley Journals; Oxford University Press Archive; Alma/SFX Local Collection |
| subjects | amino acid sequences Bacteriology Biological and medical sciences Conserved Diazotroph DNA sequence Fundamental and applied biological sciences. Psychology genes genetic code genetic techniques and protocols Genetics loci Microbiology molecular genetics molecular sequence data nucleotide sequences open reading frames ORF1 Rhizobium Rhizobium meliloti sequence homology |
| title | Molecular analysis of an essential gene upstream of rpoN in Rhizobium NGR234 |
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