Polysaccharides immobilized in polypyrrole matrices are able to induce osteogenic differentiation in mouse mesenchymal stem cells
Bone marrow mesenchymal stem cells (MSCs) have attracted considerable interest due to their ability to differentiate and contribute to the regeneration of mesenchymal tissues. The present study illustrates that the proper immobilization of heparin (Hep) and hyaluronic acid (HA) into a polypyrrole (P...
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| Veröffentlicht in: | Journal of tissue engineering and regenerative medicine 2014-12, Vol.8 (12), p.989-999 |
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| creator | Serra Moreno, Judith Sabbieti, Maria Giovanna Agas, Dimitrios Marchetti, Luigi Panero, Stefania |
| description | Bone marrow mesenchymal stem cells (MSCs) have attracted considerable interest due to their ability to differentiate and contribute to the regeneration of mesenchymal tissues. The present study illustrates that the proper immobilization of heparin (Hep) and hyaluronic acid (HA) into a polypyrrole (PPy) matrix by electropolymerization results in an optimal interface for MSC differentiation towards osteoblast lineage. The obtained thin films showed good thermal stability, hydrophilicity and slow controlled polysaccharide release. The in vitro tests showed the main role of the interface chemical composition. Indeed, PPyHep and PPyHA thin films were able to induce osteogenic differentiation as determined by levels of specific early osteogenic markers (Runx2 and osterix) even in the absence of differentiating medium. Increased levels of ALP and Alizarin red staining, both indicating mineralization processes, confirmed the presence of mature osteoblasts. Copyright © 2012 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/term.1601 |
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The present study illustrates that the proper immobilization of heparin (Hep) and hyaluronic acid (HA) into a polypyrrole (PPy) matrix by electropolymerization results in an optimal interface for MSC differentiation towards osteoblast lineage. The obtained thin films showed good thermal stability, hydrophilicity and slow controlled polysaccharide release. The in vitro tests showed the main role of the interface chemical composition. Indeed, PPyHep and PPyHA thin films were able to induce osteogenic differentiation as determined by levels of specific early osteogenic markers (Runx2 and osterix) even in the absence of differentiating medium. Increased levels of ALP and Alizarin red staining, both indicating mineralization processes, confirmed the presence of mature osteoblasts. 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The present study illustrates that the proper immobilization of heparin (Hep) and hyaluronic acid (HA) into a polypyrrole (PPy) matrix by electropolymerization results in an optimal interface for MSC differentiation towards osteoblast lineage. The obtained thin films showed good thermal stability, hydrophilicity and slow controlled polysaccharide release. The in vitro tests showed the main role of the interface chemical composition. Indeed, PPyHep and PPyHA thin films were able to induce osteogenic differentiation as determined by levels of specific early osteogenic markers (Runx2 and osterix) even in the absence of differentiating medium. Increased levels of ALP and Alizarin red staining, both indicating mineralization processes, confirmed the presence of mature osteoblasts. Copyright © 2012 John Wiley & Sons, Ltd.</description><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>Blotting, Western</subject><subject>Bone and Bones - cytology</subject><subject>Bone and Bones - drug effects</subject><subject>Cell Differentiation - drug effects</subject><subject>differentiation</subject><subject>Fluorescent Antibody Technique</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - drug effects</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>MSC</subject><subject>Polymers - chemistry</subject><subject>polypyrrole</subject><subject>polysaccharide</subject><subject>Polysaccharides - chemistry</subject><subject>Polysaccharides - pharmacology</subject><subject>Pyrroles - chemistry</subject><subject>Regenerative medicine</subject><subject>release</subject><subject>Tissue engineering</subject><issn>1932-6254</issn><issn>1932-7005</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU9v1DAQxS0EoqVw4AsgS1y4pPX_jY9QlQWpLbAUcbQcZ0Jd4nhrJ4L0xjevo1166GlGM783epqH0GtKjikh7GSEFI6pIvQJOqSas2pFiHy67xWT4gC9yPmmDKWS_Dk6YEzrFZH1Ifr3NfZzts5d2-RbyNiHEBvf-ztosR_wtqy3c0qxBxzsmLwrjE2AbVMmYyxMOznAMY8Qf8HgHW5910GCYfR29HFYroQ45aKHDIO7noPtccEDdtD3-SV61tk-w6t9PUI_Pp5dnX6qzr-sP5--P6-c0JRWEjpLGGm4toJxLYhSltWgBbSd0jUTbctq2uiusR1XrtGOUq4VVU4J1TjKj9C73d1tircT5NEEnxcHdoBiz1DFBasl06Kgbx-hN3FKQ3FXKKYlIzVZqDd7amoCtGabfLBpNv-fW4CTHfDH9zA_7CkxS2pmSc0sqZmrs83F0hRFtVP48p-_Dwqbfhu14itpfl6uzYfN9_XlN70xmt8DmX6bTg</recordid><startdate>201412</startdate><enddate>201412</enddate><creator>Serra Moreno, Judith</creator><creator>Sabbieti, Maria Giovanna</creator><creator>Agas, Dimitrios</creator><creator>Marchetti, Luigi</creator><creator>Panero, Stefania</creator><general>Blackwell Publishing Ltd</general><general>Hindawi Limited</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201412</creationdate><title>Polysaccharides immobilized in polypyrrole matrices are able to induce osteogenic differentiation in mouse mesenchymal stem cells</title><author>Serra Moreno, Judith ; Sabbieti, Maria Giovanna ; Agas, Dimitrios ; Marchetti, Luigi ; Panero, Stefania</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4911-5efa020b39a42394066a28e94edf69824dd281b9fbaf36cb9c1139616c646bc13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Alkaline Phosphatase - metabolism</topic><topic>Animals</topic><topic>Blotting, Western</topic><topic>Bone and Bones - cytology</topic><topic>Bone and Bones - drug effects</topic><topic>Cell Differentiation - drug effects</topic><topic>differentiation</topic><topic>Fluorescent Antibody Technique</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - drug effects</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred ICR</topic><topic>MSC</topic><topic>Polymers - chemistry</topic><topic>polypyrrole</topic><topic>polysaccharide</topic><topic>Polysaccharides - chemistry</topic><topic>Polysaccharides - pharmacology</topic><topic>Pyrroles - chemistry</topic><topic>Regenerative medicine</topic><topic>release</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Serra Moreno, Judith</creatorcontrib><creatorcontrib>Sabbieti, Maria Giovanna</creatorcontrib><creatorcontrib>Agas, Dimitrios</creatorcontrib><creatorcontrib>Marchetti, Luigi</creatorcontrib><creatorcontrib>Panero, Stefania</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of tissue engineering and regenerative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Serra Moreno, Judith</au><au>Sabbieti, Maria Giovanna</au><au>Agas, Dimitrios</au><au>Marchetti, Luigi</au><au>Panero, Stefania</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polysaccharides immobilized in polypyrrole matrices are able to induce osteogenic differentiation in mouse mesenchymal stem cells</atitle><jtitle>Journal of tissue engineering and regenerative medicine</jtitle><addtitle>J Tissue Eng Regen Med</addtitle><date>2014-12</date><risdate>2014</risdate><volume>8</volume><issue>12</issue><spage>989</spage><epage>999</epage><pages>989-999</pages><issn>1932-6254</issn><eissn>1932-7005</eissn><abstract>Bone marrow mesenchymal stem cells (MSCs) have attracted considerable interest due to their ability to differentiate and contribute to the regeneration of mesenchymal tissues. The present study illustrates that the proper immobilization of heparin (Hep) and hyaluronic acid (HA) into a polypyrrole (PPy) matrix by electropolymerization results in an optimal interface for MSC differentiation towards osteoblast lineage. The obtained thin films showed good thermal stability, hydrophilicity and slow controlled polysaccharide release. The in vitro tests showed the main role of the interface chemical composition. Indeed, PPyHep and PPyHA thin films were able to induce osteogenic differentiation as determined by levels of specific early osteogenic markers (Runx2 and osterix) even in the absence of differentiating medium. Increased levels of ALP and Alizarin red staining, both indicating mineralization processes, confirmed the presence of mature osteoblasts. 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| subjects | Alkaline Phosphatase - metabolism Animals Blotting, Western Bone and Bones - cytology Bone and Bones - drug effects Cell Differentiation - drug effects differentiation Fluorescent Antibody Technique Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - drug effects Mesenchymal Stromal Cells - metabolism Mice Mice, Inbred ICR MSC Polymers - chemistry polypyrrole polysaccharide Polysaccharides - chemistry Polysaccharides - pharmacology Pyrroles - chemistry Regenerative medicine release Tissue engineering |
| title | Polysaccharides immobilized in polypyrrole matrices are able to induce osteogenic differentiation in mouse mesenchymal stem cells |
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