Linezolid-resistant Staphylococcus haemolyticus and Staphylococcus hominis : single and double mutations at the domain V of 23S rRNA among isolates from a Rio de Janeiro hospital
Abstract In this work, the molecular and phenotypic antimicrobial resistance and clonal diversity of 10 linezolid-resistant Staphylococcus spp. isolates were investigated. The 7 Staphylococcus haemolyticus isolates presented Staphylococcal cassete chromosome mec (SCC mec ) V and belonged to the same...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 2014-12, Vol.80 (4), p.307-310 |
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| creator | Chamon, Raiane Cardoso Iorio, Natalia Lopes Pontes Cavalcante, Fernanda Sampaio Teodoro, Cristiane R.S de Oliveira, Ana Paula Chaves Maia, Fernanda dos Santos, Kátia Regina Netto |
| description | Abstract In this work, the molecular and phenotypic antimicrobial resistance and clonal diversity of 10 linezolid-resistant Staphylococcus spp. isolates were investigated. The 7 Staphylococcus haemolyticus isolates presented Staphylococcal cassete chromosome mec (SCC mec ) V and belonged to the same pulsed-field gel electrophoresis pulsotype. Their MICs for oxacillin, vancomycin, and linezolid were ≥256 μg/mL, 1–4 μg/mL, and 8–16 μg/mL, respectively. The 3 S. hominis presented MIC values 32 to >256 μg/mL, 2–4 μg/mL, and 12–24 μg/mL, and all carried the nontypeable SCC mec ( ccr 1 + mec A class) and belonged to 2 different genotypes. The cfr gene was not found, but the mutation G2603T was detected in S. haemolyticus and C2190T and G2603T in Staphylococcus hominis in 23S rRNA. This study demonstrates the spread of a linezolid-resistant S. haemolyticus genotype and, for the first time, describes the mutation C2190T among S. hominis isolates with a double mutation in Brazil. |
| doi_str_mv | 10.1016/j.diagmicrobio.2014.09.011 |
| format | Article |
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The 7 Staphylococcus haemolyticus isolates presented Staphylococcal cassete chromosome mec (SCC mec ) V and belonged to the same pulsed-field gel electrophoresis pulsotype. Their MICs for oxacillin, vancomycin, and linezolid were ≥256 μg/mL, 1–4 μg/mL, and 8–16 μg/mL, respectively. The 3 S. hominis presented MIC values 32 to >256 μg/mL, 2–4 μg/mL, and 12–24 μg/mL, and all carried the nontypeable SCC mec ( ccr 1 + mec A class) and belonged to 2 different genotypes. The cfr gene was not found, but the mutation G2603T was detected in S. haemolyticus and C2190T and G2603T in Staphylococcus hominis in 23S rRNA. This study demonstrates the spread of a linezolid-resistant S. haemolyticus genotype and, for the first time, describes the mutation C2190T among S. hominis isolates with a double mutation in Brazil.</description><identifier>ISSN: 0732-8893</identifier><identifier>EISSN: 1879-0070</identifier><identifier>DOI: 10.1016/j.diagmicrobio.2014.09.011</identifier><identifier>PMID: 25294302</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>23S rRNA gene mutation ; Acetamides - pharmacology ; Anti-Bacterial Agents - pharmacology ; Brazil ; Drug Resistance, Bacterial - drug effects ; Drug Resistance, Bacterial - genetics ; Electrophoresis, Gel, Pulsed-Field ; Hospitals ; Humans ; Infectious Disease ; Internal Medicine ; Linezolid ; Linezolid resistance ; Microbial Sensitivity Tests ; Mutation ; Oxazolidinones - pharmacology ; RNA, Ribosomal, 23S - genetics ; S. haemolyticus ; S. hominis ; Staphylococcal Infections - microbiology ; Staphylococcus haemolyticus - drug effects ; Staphylococcus haemolyticus - genetics ; Staphylococcus haemolyticus - isolation & purification ; Staphylococcus hominis - drug effects ; Staphylococcus hominis - genetics ; Staphylococcus hominis - isolation & purification</subject><ispartof>Diagnostic microbiology and infectious disease, 2014-12, Vol.80 (4), p.307-310</ispartof><rights>Elsevier Inc.</rights><rights>2014 Elsevier Inc.</rights><rights>Copyright © 2014 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c505t-f6fb65d18a78847713b41a6da7e4eee16d2be26aedca64f649ed639daa02cdb33</citedby><cites>FETCH-LOGICAL-c505t-f6fb65d18a78847713b41a6da7e4eee16d2be26aedca64f649ed639daa02cdb33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.diagmicrobio.2014.09.011$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25294302$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chamon, Raiane Cardoso</creatorcontrib><creatorcontrib>Iorio, Natalia Lopes Pontes</creatorcontrib><creatorcontrib>Cavalcante, Fernanda Sampaio</creatorcontrib><creatorcontrib>Teodoro, Cristiane R.S</creatorcontrib><creatorcontrib>de Oliveira, Ana Paula Chaves</creatorcontrib><creatorcontrib>Maia, Fernanda</creatorcontrib><creatorcontrib>dos Santos, Kátia Regina Netto</creatorcontrib><title>Linezolid-resistant Staphylococcus haemolyticus and Staphylococcus hominis : single and double mutations at the domain V of 23S rRNA among isolates from a Rio de Janeiro hospital</title><title>Diagnostic microbiology and infectious disease</title><addtitle>Diagn Microbiol Infect Dis</addtitle><description>Abstract In this work, the molecular and phenotypic antimicrobial resistance and clonal diversity of 10 linezolid-resistant Staphylococcus spp. isolates were investigated. The 7 Staphylococcus haemolyticus isolates presented Staphylococcal cassete chromosome mec (SCC mec ) V and belonged to the same pulsed-field gel electrophoresis pulsotype. Their MICs for oxacillin, vancomycin, and linezolid were ≥256 μg/mL, 1–4 μg/mL, and 8–16 μg/mL, respectively. The 3 S. hominis presented MIC values 32 to >256 μg/mL, 2–4 μg/mL, and 12–24 μg/mL, and all carried the nontypeable SCC mec ( ccr 1 + mec A class) and belonged to 2 different genotypes. The cfr gene was not found, but the mutation G2603T was detected in S. haemolyticus and C2190T and G2603T in Staphylococcus hominis in 23S rRNA. This study demonstrates the spread of a linezolid-resistant S. haemolyticus genotype and, for the first time, describes the mutation C2190T among S. hominis isolates with a double mutation in Brazil.</description><subject>23S rRNA gene mutation</subject><subject>Acetamides - pharmacology</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Brazil</subject><subject>Drug Resistance, Bacterial - drug effects</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Infectious Disease</subject><subject>Internal Medicine</subject><subject>Linezolid</subject><subject>Linezolid resistance</subject><subject>Microbial Sensitivity Tests</subject><subject>Mutation</subject><subject>Oxazolidinones - pharmacology</subject><subject>RNA, Ribosomal, 23S - genetics</subject><subject>S. haemolyticus</subject><subject>S. hominis</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcus haemolyticus - drug effects</subject><subject>Staphylococcus haemolyticus - genetics</subject><subject>Staphylococcus haemolyticus - isolation & purification</subject><subject>Staphylococcus hominis - drug effects</subject><subject>Staphylococcus hominis - genetics</subject><subject>Staphylococcus hominis - isolation & purification</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNksuOEzEQRVsIxISBX0AWKzYd_Oj0YxZIo-GtCKQJsLWq7erEwW1nbDdS-Cy-EDcZEIINK5ftW3VdPlUUTxhdMsrqZ_ulNrAdjQq-N37JKauWtFtSxu4UC9Y2XUlpQ-8WC9oIXrZtJ86KBzHuKWW8q-j94oyvciAoXxTf18bhN2-NLgNGExO4RDYJDruj9corNUWyAxy9PSYzb8Dpf-79aJyJ5IJE47YWf2q0n_ocjlOCZLzLiYmkHebzEYwjn4kfCBcbEq7fXxIYvdsSE72FhJEMwY8EyLXxRCN5Bw5N8NkmHkwC-7C4N4CN-Oh2PS8-vXr58epNuf7w-u3V5bpUK7pK5VAPfb3SrIWmbaumYaKvGNQaGqwQkdWa98hrQK2groa66lDXotMAlCvdC3FePD3VPQR_M2FMcjRRobX5PX6KktWi4k1H6Sy9OEkzkhgDDvIQzAjhKBmVMzO5l38ykzMzSTuZmeXkx7c-Uz-i_p36C1IWvDgJMHf71WCQURl0CrUJqJLU3vyfz_O_yiibuSmwX_CIce-n4PJ_SiYjl1Ru5umZh4dVucVGCPEDgK_IIg</recordid><startdate>20141201</startdate><enddate>20141201</enddate><creator>Chamon, Raiane Cardoso</creator><creator>Iorio, Natalia Lopes Pontes</creator><creator>Cavalcante, Fernanda Sampaio</creator><creator>Teodoro, Cristiane R.S</creator><creator>de Oliveira, Ana Paula Chaves</creator><creator>Maia, Fernanda</creator><creator>dos Santos, Kátia Regina Netto</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20141201</creationdate><title>Linezolid-resistant Staphylococcus haemolyticus and Staphylococcus hominis : single and double mutations at the domain V of 23S rRNA among isolates from a Rio de Janeiro hospital</title><author>Chamon, Raiane Cardoso ; Iorio, Natalia Lopes Pontes ; Cavalcante, Fernanda Sampaio ; Teodoro, Cristiane R.S ; de Oliveira, Ana Paula Chaves ; Maia, Fernanda ; dos Santos, Kátia Regina Netto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c505t-f6fb65d18a78847713b41a6da7e4eee16d2be26aedca64f649ed639daa02cdb33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>23S rRNA gene mutation</topic><topic>Acetamides - pharmacology</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Brazil</topic><topic>Drug Resistance, Bacterial - drug effects</topic><topic>Drug Resistance, Bacterial - genetics</topic><topic>Electrophoresis, Gel, Pulsed-Field</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Infectious Disease</topic><topic>Internal Medicine</topic><topic>Linezolid</topic><topic>Linezolid resistance</topic><topic>Microbial Sensitivity Tests</topic><topic>Mutation</topic><topic>Oxazolidinones - pharmacology</topic><topic>RNA, Ribosomal, 23S - genetics</topic><topic>S. haemolyticus</topic><topic>S. hominis</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcus haemolyticus - drug effects</topic><topic>Staphylococcus haemolyticus - genetics</topic><topic>Staphylococcus haemolyticus - isolation & purification</topic><topic>Staphylococcus hominis - drug effects</topic><topic>Staphylococcus hominis - genetics</topic><topic>Staphylococcus hominis - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chamon, Raiane Cardoso</creatorcontrib><creatorcontrib>Iorio, Natalia Lopes Pontes</creatorcontrib><creatorcontrib>Cavalcante, Fernanda Sampaio</creatorcontrib><creatorcontrib>Teodoro, Cristiane R.S</creatorcontrib><creatorcontrib>de Oliveira, Ana Paula Chaves</creatorcontrib><creatorcontrib>Maia, Fernanda</creatorcontrib><creatorcontrib>dos Santos, Kátia Regina Netto</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chamon, Raiane Cardoso</au><au>Iorio, Natalia Lopes Pontes</au><au>Cavalcante, Fernanda Sampaio</au><au>Teodoro, Cristiane R.S</au><au>de Oliveira, Ana Paula Chaves</au><au>Maia, Fernanda</au><au>dos Santos, Kátia Regina Netto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Linezolid-resistant Staphylococcus haemolyticus and Staphylococcus hominis : single and double mutations at the domain V of 23S rRNA among isolates from a Rio de Janeiro hospital</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>2014-12-01</date><risdate>2014</risdate><volume>80</volume><issue>4</issue><spage>307</spage><epage>310</epage><pages>307-310</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><abstract>Abstract In this work, the molecular and phenotypic antimicrobial resistance and clonal diversity of 10 linezolid-resistant Staphylococcus spp. isolates were investigated. The 7 Staphylococcus haemolyticus isolates presented Staphylococcal cassete chromosome mec (SCC mec ) V and belonged to the same pulsed-field gel electrophoresis pulsotype. Their MICs for oxacillin, vancomycin, and linezolid were ≥256 μg/mL, 1–4 μg/mL, and 8–16 μg/mL, respectively. The 3 S. hominis presented MIC values 32 to >256 μg/mL, 2–4 μg/mL, and 12–24 μg/mL, and all carried the nontypeable SCC mec ( ccr 1 + mec A class) and belonged to 2 different genotypes. The cfr gene was not found, but the mutation G2603T was detected in S. haemolyticus and C2190T and G2603T in Staphylococcus hominis in 23S rRNA. This study demonstrates the spread of a linezolid-resistant S. haemolyticus genotype and, for the first time, describes the mutation C2190T among S. hominis isolates with a double mutation in Brazil.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>25294302</pmid><doi>10.1016/j.diagmicrobio.2014.09.011</doi><tpages>4</tpages></addata></record> |
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| ispartof | Diagnostic microbiology and infectious disease, 2014-12, Vol.80 (4), p.307-310 |
| issn | 0732-8893 1879-0070 |
| language | eng |
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| subjects | 23S rRNA gene mutation Acetamides - pharmacology Anti-Bacterial Agents - pharmacology Brazil Drug Resistance, Bacterial - drug effects Drug Resistance, Bacterial - genetics Electrophoresis, Gel, Pulsed-Field Hospitals Humans Infectious Disease Internal Medicine Linezolid Linezolid resistance Microbial Sensitivity Tests Mutation Oxazolidinones - pharmacology RNA, Ribosomal, 23S - genetics S. haemolyticus S. hominis Staphylococcal Infections - microbiology Staphylococcus haemolyticus - drug effects Staphylococcus haemolyticus - genetics Staphylococcus haemolyticus - isolation & purification Staphylococcus hominis - drug effects Staphylococcus hominis - genetics Staphylococcus hominis - isolation & purification |
| title | Linezolid-resistant Staphylococcus haemolyticus and Staphylococcus hominis : single and double mutations at the domain V of 23S rRNA among isolates from a Rio de Janeiro hospital |
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