Integrated Solid-Phase Extraction–Capillary Liquid Chromatography (speLC) Interfaced to ESI–MS/MS for Fast Characterization and Quantification of Protein and Proteomes

The high peptide sequencing speed provided by modern hybrid tandem mass spectrometers enables the utilization of fast liquid chromatographic (LC) separation techniques. We present a robust solid-phase extraction/capillary LC system (speLC) for 5–10 min separation of semicomplex peptide mixtures prio...

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Veröffentlicht in:	Journal of proteome research 2014-12, Vol.13 (12), p.6169-6175
Hauptverfasser:	Falkenby, Lasse Gaarde, Such-Sanmartín, Gerard, Larsen, Martin R, Vorm, Ole, Bache, Nicolai, Jensen, Ole N
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Blood Proteins - metabolism Chromatography, Liquid - instrumentation Chromatography, Liquid - methods HeLa Cells Humans Peptides - metabolism Proteome - isolation & purification Proteome - metabolism Proteomics - methods Reproducibility of Results Solid Phase Extraction - methods Spectrometry, Mass, Electrospray Ionization - instrumentation Spectrometry, Mass, Electrospray Ionization - methods Tandem Mass Spectrometry - instrumentation Tandem Mass Spectrometry - methods
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