Pallidifloside D, a saponin glycoside constituent from Smilax riparia, resist to hyperuricemia based on URAT1 and GLUT9 in hyperuricemic mice
The roots and rhizomes of Smilax riparia (SR), called “Niu-Wei-Cai” in traditional Chinese medicine (TCM), are believed to be effective in treating hyperuricemia and gout symptoms. This study was designed to isolate a saponin glycoside named pallidifloside D from the total saponins of Smilax riparia...
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| Veröffentlicht in: | Journal of ethnopharmacology 2014-11, Vol.157, p.201-205 |
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| description | The roots and rhizomes of Smilax riparia (SR), called “Niu-Wei-Cai” in traditional Chinese medicine (TCM), are believed to be effective in treating hyperuricemia and gout symptoms. This study was designed to isolate a saponin glycoside named pallidifloside D from the total saponins of Smilax riparia and to examine its effect in reducing serum uric acid levels in a hyperuricemic mouse model induced by potassium oxonate.
We examined the effects of pallidifloside D treated with 5, 10 and 20mg/kg on serum uric acid levels (SUA), Serum creatinine (SCr) and blood urea nitrogen (BUN) levels in a hyperuricemic mouse. A colorimetric method was used to evaluate the effects of pallidifloside D on the XOD activities, and Western Blotting analysis were carried out to observe protein levels of mURAT1, mGLUT9 and mOTA1 in hyperuricemic mice after treatment with pallidifloside D.
The levels of serum uric acid levels (SUA) were suppressed significantly with dose-dependence by pallidifloside D treated with 5, 10 and 20mg/kg (p |
| doi_str_mv | 10.1016/j.jep.2014.09.034 |
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We examined the effects of pallidifloside D treated with 5, 10 and 20mg/kg on serum uric acid levels (SUA), Serum creatinine (SCr) and blood urea nitrogen (BUN) levels in a hyperuricemic mouse. A colorimetric method was used to evaluate the effects of pallidifloside D on the XOD activities, and Western Blotting analysis were carried out to observe protein levels of mURAT1, mGLUT9 and mOTA1 in hyperuricemic mice after treatment with pallidifloside D.
The levels of serum uric acid levels (SUA) were suppressed significantly with dose-dependence by pallidifloside D treated with 5, 10 and 20mg/kg (p<0.05, p<0.01 and p<0.01 respectively). Pallidifloside D could down-regulate the expression levels of renal mURAT1 protein in hyperuricemic mice in a dose-dependent manner (p<0.05, p<0.01, and p<0.001 respectively), and the protein levels of mGLUT9 could be down-regulated with dose-dependence (p<0.05 and p<0.01 respectively) by pallidifloside D at the dose of 10 and 20mg/kg.
These results suggest that pallidifloside D possesses a potent uricosuric effect in hyperuricemic mice through decreasing renal mURAT1 and GLUT9, which contribute to the enhancement of uric acid excretion and attenuate hyperuricemia-induced renal dysfunction.
[Display omitted]]]></description><identifier>ISSN: 0378-8741</identifier><identifier>EISSN: 1872-7573</identifier><identifier>DOI: 10.1016/j.jep.2014.09.034</identifier><identifier>PMID: 25267580</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Animals ; Disease Models, Animal ; Dose-Response Relationship, Drug ; Down-Regulation - drug effects ; Glucose Transport Proteins, Facilitative - genetics ; GLUT9 ; Hyperuricemia ; Hyperuricemia - drug therapy ; Male ; Medicine, Chinese Traditional ; Mice ; Organic Anion Transporters - genetics ; Oxonic Acid - toxicity ; Pallidifloside D ; Plant Roots ; Rhizome ; Saponin glycoside ; Saponins - administration & dosage ; Saponins - isolation & purification ; Saponins - pharmacology ; Smilax - chemistry ; Smilax riparia ; URAT1 ; Uric Acid - blood</subject><ispartof>Journal of ethnopharmacology, 2014-11, Vol.157, p.201-205</ispartof><rights>2014 Elsevier Ireland Ltd</rights><rights>Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-706dec66e23391bc56a11cef98071c613f06f6161ec09642ca08a2d69ecae51e3</citedby><cites>FETCH-LOGICAL-c353t-706dec66e23391bc56a11cef98071c613f06f6161ec09642ca08a2d69ecae51e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jep.2014.09.034$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25267580$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Xiao-Hui</creatorcontrib><creatorcontrib>Ruan, Jin-Lan</creatorcontrib><creatorcontrib>Zhang, Jun</creatorcontrib><creatorcontrib>Wang, Shu-Qing</creatorcontrib><creatorcontrib>Zhang, Yan-Wen</creatorcontrib><title>Pallidifloside D, a saponin glycoside constituent from Smilax riparia, resist to hyperuricemia based on URAT1 and GLUT9 in hyperuricemic mice</title><title>Journal of ethnopharmacology</title><addtitle>J Ethnopharmacol</addtitle><description><![CDATA[The roots and rhizomes of Smilax riparia (SR), called “Niu-Wei-Cai” in traditional Chinese medicine (TCM), are believed to be effective in treating hyperuricemia and gout symptoms. This study was designed to isolate a saponin glycoside named pallidifloside D from the total saponins of Smilax riparia and to examine its effect in reducing serum uric acid levels in a hyperuricemic mouse model induced by potassium oxonate.
We examined the effects of pallidifloside D treated with 5, 10 and 20mg/kg on serum uric acid levels (SUA), Serum creatinine (SCr) and blood urea nitrogen (BUN) levels in a hyperuricemic mouse. A colorimetric method was used to evaluate the effects of pallidifloside D on the XOD activities, and Western Blotting analysis were carried out to observe protein levels of mURAT1, mGLUT9 and mOTA1 in hyperuricemic mice after treatment with pallidifloside D.
The levels of serum uric acid levels (SUA) were suppressed significantly with dose-dependence by pallidifloside D treated with 5, 10 and 20mg/kg (p<0.05, p<0.01 and p<0.01 respectively). Pallidifloside D could down-regulate the expression levels of renal mURAT1 protein in hyperuricemic mice in a dose-dependent manner (p<0.05, p<0.01, and p<0.001 respectively), and the protein levels of mGLUT9 could be down-regulated with dose-dependence (p<0.05 and p<0.01 respectively) by pallidifloside D at the dose of 10 and 20mg/kg.
These results suggest that pallidifloside D possesses a potent uricosuric effect in hyperuricemic mice through decreasing renal mURAT1 and GLUT9, which contribute to the enhancement of uric acid excretion and attenuate hyperuricemia-induced renal dysfunction.
[Display omitted]]]></description><subject>Animals</subject><subject>Disease Models, Animal</subject><subject>Dose-Response Relationship, Drug</subject><subject>Down-Regulation - drug effects</subject><subject>Glucose Transport Proteins, Facilitative - genetics</subject><subject>GLUT9</subject><subject>Hyperuricemia</subject><subject>Hyperuricemia - drug therapy</subject><subject>Male</subject><subject>Medicine, Chinese Traditional</subject><subject>Mice</subject><subject>Organic Anion Transporters - genetics</subject><subject>Oxonic Acid - toxicity</subject><subject>Pallidifloside D</subject><subject>Plant Roots</subject><subject>Rhizome</subject><subject>Saponin glycoside</subject><subject>Saponins - administration & dosage</subject><subject>Saponins - isolation & purification</subject><subject>Saponins - pharmacology</subject><subject>Smilax - chemistry</subject><subject>Smilax riparia</subject><subject>URAT1</subject><subject>Uric Acid - blood</subject><issn>0378-8741</issn><issn>1872-7573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcGO1DAQRC0EYoeFD-CCfOSwCd1xYifitFpgQRoJBDNny2N3wKMkDnaCmI_gn_FqFsSJQ6ul1quSqoux5wglAspXx_JIc1kB1iV0JYj6Adtgq6pCNUo8ZBsQqi1aVeMFe5LSEQAU1vCYXVRNJVXTwob9-mSGwTvfDyF5R_zNFTc8mTlMfuJfh5M9n22Y0uKXlaaF9zGM_MvoB_OTRz-b6M0Vj5R8WvgS-LfTTHGN3tLoDT-YRI6Hie8_X--Qm8nx2-1-1_Fs_y9peR56yh71Zkj07H5fsv27t7ub98X24-2Hm-ttYUUjlkKBdGSlpEqIDg-2kQbRUt-1OaCVKHqQvUSJZKGTdWUNtKZysiNrqEESl-zl2XeO4ftKadGjT5aGwUwU1qRRirpStULMKJ5RG0NKkXo9Rz-aeNII-q4FfdS5BX3XgoZO5xay5sW9_XoYyf1V_Hl7Bl6fAcohf3iKOllPkyXnI9lFu-D_Y_8bwOeZCg</recordid><startdate>20141118</startdate><enddate>20141118</enddate><creator>Wu, Xiao-Hui</creator><creator>Ruan, Jin-Lan</creator><creator>Zhang, Jun</creator><creator>Wang, Shu-Qing</creator><creator>Zhang, Yan-Wen</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20141118</creationdate><title>Pallidifloside D, a saponin glycoside constituent from Smilax riparia, resist to hyperuricemia based on URAT1 and GLUT9 in hyperuricemic mice</title><author>Wu, Xiao-Hui ; Ruan, Jin-Lan ; Zhang, Jun ; Wang, Shu-Qing ; Zhang, Yan-Wen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-706dec66e23391bc56a11cef98071c613f06f6161ec09642ca08a2d69ecae51e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Disease Models, Animal</topic><topic>Dose-Response Relationship, Drug</topic><topic>Down-Regulation - drug effects</topic><topic>Glucose Transport Proteins, Facilitative - genetics</topic><topic>GLUT9</topic><topic>Hyperuricemia</topic><topic>Hyperuricemia - drug therapy</topic><topic>Male</topic><topic>Medicine, Chinese Traditional</topic><topic>Mice</topic><topic>Organic Anion Transporters - genetics</topic><topic>Oxonic Acid - toxicity</topic><topic>Pallidifloside D</topic><topic>Plant Roots</topic><topic>Rhizome</topic><topic>Saponin glycoside</topic><topic>Saponins - administration & dosage</topic><topic>Saponins - isolation & purification</topic><topic>Saponins - pharmacology</topic><topic>Smilax - chemistry</topic><topic>Smilax riparia</topic><topic>URAT1</topic><topic>Uric Acid - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Xiao-Hui</creatorcontrib><creatorcontrib>Ruan, Jin-Lan</creatorcontrib><creatorcontrib>Zhang, Jun</creatorcontrib><creatorcontrib>Wang, Shu-Qing</creatorcontrib><creatorcontrib>Zhang, Yan-Wen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of ethnopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Xiao-Hui</au><au>Ruan, Jin-Lan</au><au>Zhang, Jun</au><au>Wang, Shu-Qing</au><au>Zhang, Yan-Wen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pallidifloside D, a saponin glycoside constituent from Smilax riparia, resist to hyperuricemia based on URAT1 and GLUT9 in hyperuricemic mice</atitle><jtitle>Journal of ethnopharmacology</jtitle><addtitle>J Ethnopharmacol</addtitle><date>2014-11-18</date><risdate>2014</risdate><volume>157</volume><spage>201</spage><epage>205</epage><pages>201-205</pages><issn>0378-8741</issn><eissn>1872-7573</eissn><abstract><![CDATA[The roots and rhizomes of Smilax riparia (SR), called “Niu-Wei-Cai” in traditional Chinese medicine (TCM), are believed to be effective in treating hyperuricemia and gout symptoms. This study was designed to isolate a saponin glycoside named pallidifloside D from the total saponins of Smilax riparia and to examine its effect in reducing serum uric acid levels in a hyperuricemic mouse model induced by potassium oxonate.
We examined the effects of pallidifloside D treated with 5, 10 and 20mg/kg on serum uric acid levels (SUA), Serum creatinine (SCr) and blood urea nitrogen (BUN) levels in a hyperuricemic mouse. A colorimetric method was used to evaluate the effects of pallidifloside D on the XOD activities, and Western Blotting analysis were carried out to observe protein levels of mURAT1, mGLUT9 and mOTA1 in hyperuricemic mice after treatment with pallidifloside D.
The levels of serum uric acid levels (SUA) were suppressed significantly with dose-dependence by pallidifloside D treated with 5, 10 and 20mg/kg (p<0.05, p<0.01 and p<0.01 respectively). Pallidifloside D could down-regulate the expression levels of renal mURAT1 protein in hyperuricemic mice in a dose-dependent manner (p<0.05, p<0.01, and p<0.001 respectively), and the protein levels of mGLUT9 could be down-regulated with dose-dependence (p<0.05 and p<0.01 respectively) by pallidifloside D at the dose of 10 and 20mg/kg.
These results suggest that pallidifloside D possesses a potent uricosuric effect in hyperuricemic mice through decreasing renal mURAT1 and GLUT9, which contribute to the enhancement of uric acid excretion and attenuate hyperuricemia-induced renal dysfunction.
[Display omitted]]]></abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>25267580</pmid><doi>10.1016/j.jep.2014.09.034</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-8741 |
| ispartof | Journal of ethnopharmacology, 2014-11, Vol.157, p.201-205 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Disease Models, Animal Dose-Response Relationship, Drug Down-Regulation - drug effects Glucose Transport Proteins, Facilitative - genetics GLUT9 Hyperuricemia Hyperuricemia - drug therapy Male Medicine, Chinese Traditional Mice Organic Anion Transporters - genetics Oxonic Acid - toxicity Pallidifloside D Plant Roots Rhizome Saponin glycoside Saponins - administration & dosage Saponins - isolation & purification Saponins - pharmacology Smilax - chemistry Smilax riparia URAT1 Uric Acid - blood |
| title | Pallidifloside D, a saponin glycoside constituent from Smilax riparia, resist to hyperuricemia based on URAT1 and GLUT9 in hyperuricemic mice |
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