Absorption and emission spectroscopic characterization of photo-dynamics of photoactivated adenylyl cyclase mutant bPAC-Y7F of Beggiatoa sp

•Mutation changes singlet BLUF domain photo-cycle to triplet based photo-dynamics.•Three conformations of SUMO-bPAC-Y7F with different photo-dynamics are observed.•First fraction follows triplet based flavin semiquinone photo-cycle dynamics.•Second fraction forms irreversible flavin hydroquinone cha...

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Veröffentlicht in:	Journal of photochemistry and photobiology. B, Biology Biology, 2014-11, Vol.140, p.182-193
Hauptverfasser:	Penzkofer, Alfons, Stierl, Manuela, Mathes, Tilo, Hegemann, Peter
Format:	Artikel
Sprache:	eng
Schlagworte:	Absorption, Physicochemical Adenylyl Cyclases - chemistry Adenylyl Cyclases - genetics Adenylyl Cyclases - metabolism Amino Acid Sequence Anionic flavin hydoquinone charge transfer complex formation Beggiatoa - enzymology BLUF domain Dinitrocresols - metabolism Enzyme Activation - radiation effects Flavin-C4a-adduct photo-cycle Light Models, Molecular Molecular Sequence Data Mutation Photoactivated cyclase bPAC Protein Structure, Tertiary Soil bacterium Beggiatoa sp Spectrum Analysis Temperature Triplet based flavin semiquinone photo-cycle
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