Optimization of pancreatic trypsin extraction in PEG/citrate aqueous two-phase systems

•Statistical design proved to be adequate for pancreatic trypsin extraction in ATPS.•The maximum compromise between trypsin purification factor and yield were obtained.•Pancreatic trypsin was obtained with a PF greater than 2 and a Y of 99.7%. Enzyme extraction using aqueous two-phase systems (ATPS)...

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Veröffentlicht in:	Protein expression and purification 2015-02, Vol.106, p.66-71
Hauptverfasser:	Pérez, Rocío L., Loureiro, Dana B., Nerli, Bibiana B., Tubio, Gisela
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Aqueous two-phase systems Biochemistry - methods Cattle Citrates - chemistry Extraction Hydrogen-Ion Concentration Pancreas - enzymology Pancreatic Extracts - metabolism Pancreatic trypsin Polyethylene Glycols - chemistry Regression Analysis Reproducibility of Results Statistic optimization Trypsin - isolation & purification Water
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