Histochemical localization of non-sulphated and sulphated polysaccharides of the red alga Hypnea musciformis
Specific chemical staining was used for the localization of polysaccharides in plant sections. Light microscope studies using toluidine blue staining reactions indicate that the most highly sulphated polysaccharides are probably K-carrageenan, the most highly sulphated compounds are found in the ext...
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Veröffentlicht in: | Journal of phycology 1992-01, Vol.28 |
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description | Specific chemical staining was used for the localization of polysaccharides in plant sections. Light microscope studies using toluidine blue staining reactions indicate that the most highly sulphated polysaccharides are probably K-carrageenan, the most highly sulphated compounds are found in the extra-cellular matrix and also in the intercellular regions of the sub-cortical and medullary cells. Tetraspores and carpospores had no methacromasia. The PAS reaction for polysaccharides is negative for the different regions of the cell wall. PAS reaction results in an intense staining of the carpospores and tetraspores, indicating the presence of the floridean starch grains. |
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Light microscope studies using toluidine blue staining reactions indicate that the most highly sulphated polysaccharides are probably K-carrageenan, the most highly sulphated compounds are found in the extra-cellular matrix and also in the intercellular regions of the sub-cortical and medullary cells. Tetraspores and carpospores had no methacromasia. The PAS reaction for polysaccharides is negative for the different regions of the cell wall. PAS reaction results in an intense staining of the carpospores and tetraspores, indicating the presence of the floridean starch grains.</description><identifier>ISSN: 0022-3646</identifier><language>eng</language><subject>Hypnea musciformis ; Marine</subject><ispartof>Journal of phycology, 1992-01, Vol.28</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Bouzon, Z L</creatorcontrib><creatorcontrib>Moraes, N</creatorcontrib><title>Histochemical localization of non-sulphated and sulphated polysaccharides of the red alga Hypnea musciformis</title><title>Journal of phycology</title><description>Specific chemical staining was used for the localization of polysaccharides in plant sections. Light microscope studies using toluidine blue staining reactions indicate that the most highly sulphated polysaccharides are probably K-carrageenan, the most highly sulphated compounds are found in the extra-cellular matrix and also in the intercellular regions of the sub-cortical and medullary cells. Tetraspores and carpospores had no methacromasia. The PAS reaction for polysaccharides is negative for the different regions of the cell wall. PAS reaction results in an intense staining of the carpospores and tetraspores, indicating the presence of the floridean starch grains.</description><subject>Hypnea musciformis</subject><subject>Marine</subject><issn>0022-3646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNqNyrsKwkAQheEtFIyXd9jKLrC5GO1FyQPYy7CZmJXJTsxsivj0JiDY2pyfA99CRcakaZwVebFSa5GnMeZYHJJIUekksG2wdRZIE0_r3hAce8219uxjGahrIGClwVf69zqmUcDaBnpXocw8NKj7GdIDdDl2HkG3g1hXc9862aplDSS4-3aj9tfL7VzGXc-vASXcJ2SRCDzyIPekyPIkPSXZ3_ADndZM7Q</recordid><startdate>19920101</startdate><enddate>19920101</enddate><creator>Bouzon, Z L</creator><creator>Moraes, N</creator><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H97</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope></search><sort><creationdate>19920101</creationdate><title>Histochemical localization of non-sulphated and sulphated polysaccharides of the red alga Hypnea musciformis</title><author>Bouzon, Z L ; Moraes, N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_163412813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Hypnea musciformis</topic><topic>Marine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bouzon, Z L</creatorcontrib><creatorcontrib>Moraes, N</creatorcontrib><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of phycology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bouzon, Z L</au><au>Moraes, N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Histochemical localization of non-sulphated and sulphated polysaccharides of the red alga Hypnea musciformis</atitle><jtitle>Journal of phycology</jtitle><date>1992-01-01</date><risdate>1992</risdate><volume>28</volume><issn>0022-3646</issn><abstract>Specific chemical staining was used for the localization of polysaccharides in plant sections. Light microscope studies using toluidine blue staining reactions indicate that the most highly sulphated polysaccharides are probably K-carrageenan, the most highly sulphated compounds are found in the extra-cellular matrix and also in the intercellular regions of the sub-cortical and medullary cells. Tetraspores and carpospores had no methacromasia. The PAS reaction for polysaccharides is negative for the different regions of the cell wall. PAS reaction results in an intense staining of the carpospores and tetraspores, indicating the presence of the floridean starch grains.</abstract></addata></record> |
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subjects | Hypnea musciformis Marine |
title | Histochemical localization of non-sulphated and sulphated polysaccharides of the red alga Hypnea musciformis |
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