The structure and activity of two cytochrome P450 sub(c21) proteins encoded in the ovine adrenal cortex
The steroidogenic enzyme cytochrome P450 sub(c21) (CYP21A1) is synthesized in the adrenal cortex and is essential for cortisol and aldosterone production. We have studied the structure and activity of ovine P450 sub(c21) proteins by analysis and expression of the corresponding cDNAs. Two P450 sub(c2...
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| Veröffentlicht in: | The Journal of biological chemistry 1992-01, Vol.267 (23), p.16212-16218 |
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| container_title | The Journal of biological chemistry |
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| creator | Crawford, R J Hammond, V E Connell, JMC Coghlan, J P |
| description | The steroidogenic enzyme cytochrome P450 sub(c21) (CYP21A1) is synthesized in the adrenal cortex and is essential for cortisol and aldosterone production. We have studied the structure and activity of ovine P450 sub(c21) proteins by analysis and expression of the corresponding cDNAs. Two P450 sub(c21) mRNAs (2.2 and 1.7 kilobases) were detected in ovine adrenal RNA and corresponded to two types of P450 sub(c21) cDNA clones that differed in their 3' region. One clone encoded a protein similar in structure to bovine, murine, and human P450 sub(c21) proteins. The other clone contained a 3' deletion of about 500 nucleotides and encoded a P450 sub(c21) protein that was truncated by 18 residues at the carboxyl terminus. The boundaries of this deletion suggested that an additional splicing event was responsible for the shortened mRNA sequence. |
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We have studied the structure and activity of ovine P450 sub(c21) proteins by analysis and expression of the corresponding cDNAs. Two P450 sub(c21) mRNAs (2.2 and 1.7 kilobases) were detected in ovine adrenal RNA and corresponded to two types of P450 sub(c21) cDNA clones that differed in their 3' region. One clone encoded a protein similar in structure to bovine, murine, and human P450 sub(c21) proteins. The other clone contained a 3' deletion of about 500 nucleotides and encoded a P450 sub(c21) protein that was truncated by 18 residues at the carboxyl terminus. The boundaries of this deletion suggested that an additional splicing event was responsible for the shortened mRNA sequence.</description><identifier>ISSN: 0021-9258</identifier><language>eng</language><subject>adrenal glands ; amino acid sequence ; cDNA ; cYP21A1 gene ; cytochrome P450 ; genes ; nucleotide sequence ; prediction ; sheep</subject><ispartof>The Journal of biological chemistry, 1992-01, Vol.267 (23), p.16212-16218</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Crawford, R J</creatorcontrib><creatorcontrib>Hammond, V E</creatorcontrib><creatorcontrib>Connell, JMC</creatorcontrib><creatorcontrib>Coghlan, J P</creatorcontrib><title>The structure and activity of two cytochrome P450 sub(c21) proteins encoded in the ovine adrenal cortex</title><title>The Journal of biological chemistry</title><description>The steroidogenic enzyme cytochrome P450 sub(c21) (CYP21A1) is synthesized in the adrenal cortex and is essential for cortisol and aldosterone production. We have studied the structure and activity of ovine P450 sub(c21) proteins by analysis and expression of the corresponding cDNAs. Two P450 sub(c21) mRNAs (2.2 and 1.7 kilobases) were detected in ovine adrenal RNA and corresponded to two types of P450 sub(c21) cDNA clones that differed in their 3' region. One clone encoded a protein similar in structure to bovine, murine, and human P450 sub(c21) proteins. The other clone contained a 3' deletion of about 500 nucleotides and encoded a P450 sub(c21) protein that was truncated by 18 residues at the carboxyl terminus. The boundaries of this deletion suggested that an additional splicing event was responsible for the shortened mRNA sequence.</description><subject>adrenal glands</subject><subject>amino acid sequence</subject><subject>cDNA</subject><subject>cYP21A1 gene</subject><subject>cytochrome P450</subject><subject>genes</subject><subject>nucleotide sequence</subject><subject>prediction</subject><subject>sheep</subject><issn>0021-9258</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNqNzDkOAjEMheEUILHewRWCAimZBTE1AlFS0KOQMRA0xBA7LLdnCg7Aa_7m6euovtaZmVdZueypAfNVtysq01fn_QWBJSYnKSLYUIN14p9ePkAnkBeB-wi5S6Qbwq4oNXA6Tl1mZnCPJOgDAwZHNdbgA0jL0dOHlqojBtuAoyj4HqnuyTaM41-HarJZ71fbeYs8ErIcbp4dNo0NSIkPZpHnldEm__v4BckOSQI</recordid><startdate>19920101</startdate><enddate>19920101</enddate><creator>Crawford, R J</creator><creator>Hammond, V E</creator><creator>Connell, JMC</creator><creator>Coghlan, J P</creator><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19920101</creationdate><title>The structure and activity of two cytochrome P450 sub(c21) proteins encoded in the ovine adrenal cortex</title><author>Crawford, R J ; Hammond, V E ; Connell, JMC ; Coghlan, J P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_163391013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>adrenal glands</topic><topic>amino acid sequence</topic><topic>cDNA</topic><topic>cYP21A1 gene</topic><topic>cytochrome P450</topic><topic>genes</topic><topic>nucleotide sequence</topic><topic>prediction</topic><topic>sheep</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Crawford, R J</creatorcontrib><creatorcontrib>Hammond, V E</creatorcontrib><creatorcontrib>Connell, JMC</creatorcontrib><creatorcontrib>Coghlan, J P</creatorcontrib><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Crawford, R J</au><au>Hammond, V E</au><au>Connell, JMC</au><au>Coghlan, J P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The structure and activity of two cytochrome P450 sub(c21) proteins encoded in the ovine adrenal cortex</atitle><jtitle>The Journal of biological chemistry</jtitle><date>1992-01-01</date><risdate>1992</risdate><volume>267</volume><issue>23</issue><spage>16212</spage><epage>16218</epage><pages>16212-16218</pages><issn>0021-9258</issn><abstract>The steroidogenic enzyme cytochrome P450 sub(c21) (CYP21A1) is synthesized in the adrenal cortex and is essential for cortisol and aldosterone production. We have studied the structure and activity of ovine P450 sub(c21) proteins by analysis and expression of the corresponding cDNAs. Two P450 sub(c21) mRNAs (2.2 and 1.7 kilobases) were detected in ovine adrenal RNA and corresponded to two types of P450 sub(c21) cDNA clones that differed in their 3' region. One clone encoded a protein similar in structure to bovine, murine, and human P450 sub(c21) proteins. The other clone contained a 3' deletion of about 500 nucleotides and encoded a P450 sub(c21) protein that was truncated by 18 residues at the carboxyl terminus. The boundaries of this deletion suggested that an additional splicing event was responsible for the shortened mRNA sequence.</abstract></addata></record> |
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| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1992-01, Vol.267 (23), p.16212-16218 |
| issn | 0021-9258 |
| language | eng |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | adrenal glands amino acid sequence cDNA cYP21A1 gene cytochrome P450 genes nucleotide sequence prediction sheep |
| title | The structure and activity of two cytochrome P450 sub(c21) proteins encoded in the ovine adrenal cortex |
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