Bioluminescent measurement in single cardiomyocytes of sudden cytosolic ATP depletion coincident with rigor
The sequence of events that leads to irreversible injury of the ischaemic myocardium is poorly under-stood but it is axiomatic that lack of oxygen will impair regeneration of ATP. In the globally-ischaemic heart a contracture develops which is independent of raised cytoplasmic free Ca 2+ and which h...
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| Veröffentlicht in: | Journal of molecular and cellular cardiology 1992-03, Vol.24 (3), p.213-218 |
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| container_title | Journal of molecular and cellular cardiology |
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| creator | Bowers, Keith C Allshire, Ashley P Cobbold, Peter H |
| description | The sequence of events that leads to irreversible injury of the ischaemic myocardium is poorly under-stood but it is axiomatic that lack of oxygen will impair regeneration of ATP. In the globally-ischaemic heart a contracture develops which is independent of raised cytoplasmic free Ca
2+ and which has been attributed to activation of actomyosin by nucleotide-free actomyosin cross-bridges (‘rigor complexes’) which form at low ATP concentrations [1–3]. Single, metabolically-poisoned or anoxic cardiomyocytes show comparable behaviour, shortening before a significant rise in cytoplasmic free Ca
2+ occurs [4–7]. To explain the close temporal relation-ship that exists between cell shortening and the onset of the free Ca
2+ rise we have predicted [8, 9] that, during myocyte shortening, a precipitous fall in cytosolic ATP concentration occurs, the result of rigor-complexes activating myosin ATPase, which then perturbs ionic homeostasis. Here we show, by means of continuous measurements of cytosolic ATP using firefly luciferase microinjected into single, isolated cardiomyocytes, that cell shortening coincides with an abrupt fall in cytosolic ATP. |
| doi_str_mv | 10.1016/0022-2828(92)93159-H |
| format | Article |
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2+ occurs [4–7]. To explain the close temporal relation-ship that exists between cell shortening and the onset of the free Ca
2+ rise we have predicted [8, 9] that, during myocyte shortening, a precipitous fall in cytosolic ATP concentration occurs, the result of rigor-complexes activating myosin ATPase, which then perturbs ionic homeostasis. Here we show, by means of continuous measurements of cytosolic ATP using firefly luciferase microinjected into single, isolated cardiomyocytes, that cell shortening coincides with an abrupt fall in cytosolic ATP.</description><identifier>ISSN: 0022-2828</identifier><identifier>EISSN: 1095-8584</identifier><identifier>DOI: 10.1016/0022-2828(92)93159-H</identifier><identifier>PMID: 1625346</identifier><identifier>CODEN: JMCDAY</identifier><language>eng</language><publisher>Kent: Elsevier Ltd</publisher><subject>Adenosine Triphosphate - metabolism ; Animals ; ATP ; Biological and medical sciences ; cardiac muscle ; correlation ; Cytosol - metabolism ; Fundamental and applied biological sciences. Psychology ; Heart ; In Vitro Techniques ; levels ; Luciferases ; Luminescent Measurements ; Microinjections ; Myocardial Contraction - physiology ; myocardium ; Myocardium - cytology ; Myocardium - metabolism ; Rats ; Thermodynamics ; Vertebrates: cardiovascular system</subject><ispartof>Journal of molecular and cellular cardiology, 1992-03, Vol.24 (3), p.213-218</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-b60eed1c6163519094618be5cf2b53d5da31d235c7680dea544b5c2cc9d7a3a93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/002228289293159H$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5364548$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1625346$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bowers, Keith C</creatorcontrib><creatorcontrib>Allshire, Ashley P</creatorcontrib><creatorcontrib>Cobbold, Peter H</creatorcontrib><title>Bioluminescent measurement in single cardiomyocytes of sudden cytosolic ATP depletion coincident with rigor</title><title>Journal of molecular and cellular cardiology</title><addtitle>J Mol Cell Cardiol</addtitle><description>The sequence of events that leads to irreversible injury of the ischaemic myocardium is poorly under-stood but it is axiomatic that lack of oxygen will impair regeneration of ATP. In the globally-ischaemic heart a contracture develops which is independent of raised cytoplasmic free Ca
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2+ occurs [4–7]. To explain the close temporal relation-ship that exists between cell shortening and the onset of the free Ca
2+ rise we have predicted [8, 9] that, during myocyte shortening, a precipitous fall in cytosolic ATP concentration occurs, the result of rigor-complexes activating myosin ATPase, which then perturbs ionic homeostasis. Here we show, by means of continuous measurements of cytosolic ATP using firefly luciferase microinjected into single, isolated cardiomyocytes, that cell shortening coincides with an abrupt fall in cytosolic ATP.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Animals</subject><subject>ATP</subject><subject>Biological and medical sciences</subject><subject>cardiac muscle</subject><subject>correlation</subject><subject>Cytosol - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Heart</subject><subject>In Vitro Techniques</subject><subject>levels</subject><subject>Luciferases</subject><subject>Luminescent Measurements</subject><subject>Microinjections</subject><subject>Myocardial Contraction - physiology</subject><subject>myocardium</subject><subject>Myocardium - cytology</subject><subject>Myocardium - metabolism</subject><subject>Rats</subject><subject>Thermodynamics</subject><subject>Vertebrates: cardiovascular system</subject><issn>0022-2828</issn><issn>1095-8584</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQhi0EKtvCPwDJB4TKIeDvjS9IpQIWqRIcytly7EkZSOLFTkD773HYVblxsj3zzKvxQ8gzzl5zxs0bxoRoRCvaSyteWcm1bXYPyIYzq5tWt-oh2dwjj8l5Kd8ZY1ZJeUbOuBFaKrMhP95hGpYRJygBppmO4MuSYVzvONGC090ANPgcMY2HFA4zFJp6WpYYYaL1nUoaMNCr2y80wn6AGVOtJ5wCxjXlN87faMa7lJ-QR70fCjw9nRfk64f3t9e75ubzx0_XVzdNUHw7N51hAJEHw43U3NaVDW870KEXnZZRRy95FFKHrWlZBK-V6nQQIdi49dJbeUFeHnP3Of1coMxuxPq7YfATpKW4miu31UUF1REMOZWSoXf7jKPPB8eZWx27VaBbBTor3F_HblfHnp_yl26E-G_oKLX2X5z6vgQ_9NlXF-Ue09IordqKvT1iUF38QsiuBIQpQMQMYXYx4f_3-AN6wJoQ</recordid><startdate>19920301</startdate><enddate>19920301</enddate><creator>Bowers, Keith C</creator><creator>Allshire, Ashley P</creator><creator>Cobbold, Peter H</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope></search><sort><creationdate>19920301</creationdate><title>Bioluminescent measurement in single cardiomyocytes of sudden cytosolic ATP depletion coincident with rigor</title><author>Bowers, Keith C ; Allshire, Ashley P ; Cobbold, Peter H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-b60eed1c6163519094618be5cf2b53d5da31d235c7680dea544b5c2cc9d7a3a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Animals</topic><topic>ATP</topic><topic>Biological and medical sciences</topic><topic>cardiac muscle</topic><topic>correlation</topic><topic>Cytosol - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Heart</topic><topic>In Vitro Techniques</topic><topic>levels</topic><topic>Luciferases</topic><topic>Luminescent Measurements</topic><topic>Microinjections</topic><topic>Myocardial Contraction - physiology</topic><topic>myocardium</topic><topic>Myocardium - cytology</topic><topic>Myocardium - metabolism</topic><topic>Rats</topic><topic>Thermodynamics</topic><topic>Vertebrates: cardiovascular system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bowers, Keith C</creatorcontrib><creatorcontrib>Allshire, Ashley P</creatorcontrib><creatorcontrib>Cobbold, Peter H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of molecular and cellular cardiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bowers, Keith C</au><au>Allshire, Ashley P</au><au>Cobbold, Peter H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bioluminescent measurement in single cardiomyocytes of sudden cytosolic ATP depletion coincident with rigor</atitle><jtitle>Journal of molecular and cellular cardiology</jtitle><addtitle>J Mol Cell Cardiol</addtitle><date>1992-03-01</date><risdate>1992</risdate><volume>24</volume><issue>3</issue><spage>213</spage><epage>218</epage><pages>213-218</pages><issn>0022-2828</issn><eissn>1095-8584</eissn><coden>JMCDAY</coden><abstract>The sequence of events that leads to irreversible injury of the ischaemic myocardium is poorly under-stood but it is axiomatic that lack of oxygen will impair regeneration of ATP. In the globally-ischaemic heart a contracture develops which is independent of raised cytoplasmic free Ca
2+ and which has been attributed to activation of actomyosin by nucleotide-free actomyosin cross-bridges (‘rigor complexes’) which form at low ATP concentrations [1–3]. Single, metabolically-poisoned or anoxic cardiomyocytes show comparable behaviour, shortening before a significant rise in cytoplasmic free Ca
2+ occurs [4–7]. To explain the close temporal relation-ship that exists between cell shortening and the onset of the free Ca
2+ rise we have predicted [8, 9] that, during myocyte shortening, a precipitous fall in cytosolic ATP concentration occurs, the result of rigor-complexes activating myosin ATPase, which then perturbs ionic homeostasis. Here we show, by means of continuous measurements of cytosolic ATP using firefly luciferase microinjected into single, isolated cardiomyocytes, that cell shortening coincides with an abrupt fall in cytosolic ATP.</abstract><cop>Kent</cop><pub>Elsevier Ltd</pub><pmid>1625346</pmid><doi>10.1016/0022-2828(92)93159-H</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of molecular and cellular cardiology, 1992-03, Vol.24 (3), p.213-218 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adenosine Triphosphate - metabolism Animals ATP Biological and medical sciences cardiac muscle correlation Cytosol - metabolism Fundamental and applied biological sciences. Psychology Heart In Vitro Techniques levels Luciferases Luminescent Measurements Microinjections Myocardial Contraction - physiology myocardium Myocardium - cytology Myocardium - metabolism Rats Thermodynamics Vertebrates: cardiovascular system |
| title | Bioluminescent measurement in single cardiomyocytes of sudden cytosolic ATP depletion coincident with rigor |
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