Flavobacterium odoratum lipase: Isolation and characterization

An extracellular lipase from Flavobacterium odoratum was purified 14-fold with 18% recovery by three-phase partitioning, anion-exchange chromatography, and hydroxylapatite chromatography. The purified enzyme had a monomeric molecular mass of approximately 32,500. The enzyme is fairly thermostable an...

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Veröffentlicht in:	Enzyme and microbial technology 1997-07, Vol.21 (1), p.52-58
Hauptverfasser:	Labuschagne, Rachelle B., van Tonder, André, Litthauer, Derek
Format:	Artikel
Sprache:	eng
Schlagworte:	bacterial Biological and medical sciences Biotechnology Enzyme engineering Flavobacterium odoratum Fundamental and applied biological sciences. Psychology Improved methods for extraction and purification of enzymes lipase Methods. Procedures. Technologies
Online-Zugang:	Volltext
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