Imidazole--a new ligand for metal affinity precipitation: precipitation of Kunitz soybean trypsin inhibitor using Cu(II)-loaded copolymers of 1-vinylimidazole with N-vinylcaprolactam or N-isopropylacrylamide

Kunitz soybean trypsin inhibitor (STI) was specifically coprecipitated during precipitation of Cu(II)-loaded copolymers induced by increase in temperature and ionic strength. The copolymers used consisted of 1-vinylimidazole and N-vinylcaprolactam or N-isopropylacrylamide. The elution of STI was ach...

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Veröffentlicht in:	Applied biochemistry and biotechnology 1997, Vol.68 (1/2), p.121-133
Hauptverfasser:	Galaev, I.Y, Kumar, A, Agarwal, R, Gupta, M.N, Mattiasson, B
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry Biological and medical sciences Biological Sciences Biotechnology Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Others Various methods and equipments
Online-Zugang:	Volltext
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